{"id":5927,"date":"2023-03-28T15:40:10","date_gmt":"2023-03-28T07:40:10","guid":{"rendered":"http:\/\/case.ntu.edu.tw\/scinarrator\/wordpress\/?p=5927"},"modified":"2023-06-17T10:52:41","modified_gmt":"2023-06-17T02:52:41","slug":"%e3%80%902022%e5%8f%b0%e7%a9%8d%e9%9b%bb%e7%9b%83%e9%9d%92%e5%b9%b4%e5%b0%ac%e7%a7%91%e5%ad%b8%e3%80%91%e3%80%8c%e7%a7%91%e6%99%ae%e6%9b%b8%e7%b1%8d%e5%b0%8e%e8%ae%80%e6%96%87%e7%ab%b6%e8%b3%bd-25","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/scinarrator\/?p=5927","title":{"rendered":"\u30102022\u53f0\u7a4d\u96fb\u76c3\u9752\u5e74\u5c2c\u79d1\u5b78\u3011\u300c\u79d1\u666e\u66f8\u7c4d\u5c0e\u8b80\u6587\u7af6\u8cfd\u300d\u512a\u9078\u5c0e\u8b80\u6587\uff08\u8521\u4f69\u747e\uff09"},"content":{"rendered":"<p><span style=\"color: #000000;\"><strong>\u64b0\u6587\uff1a\u8521\u4f69\u747e\uff5c\u5ef6\u5e73\u9ad8\u4e2d<\/strong><\/span><\/p>\n<p><strong><span style=\"color: #000000;\">\u57fa\u56e0\u7de8\u8f2f\u7684\u5947\u5e7b\u65c5\u7a0b\uff1a\u8b80\u300a\u7834\u89e3\u57fa\u56e0\u78bc\u7684\u4eba\u300b<\/span><\/strong><\/p>\n<p><span style=\"color: #000000;\">\u662f\u4ec0\u9ebc\u8b93\u4e00\u4f4d\u8072\u8b7d\u5353\u8457\u7684\u79d1\u5b78\u5bb6\u5403\u8db3\u82e6\u982d\uff0c\u60f3\u8981\u63a8\u52d5\u751f\u6b96\u7d30\u80de\u7684\u57fa\u56e0\u6539\u9020\uff1f\u300c\u7834\u89e3\u57fa\u56e0\u5bc6\u78bc\u7684\u4eba\u300d\u771f\u7684\u53ea\u9760\u8457\u4e00\u500b\u4eba\u7684\u52aa\u529b\u5c31\u9054\u6210\u4e86\u4ed6\u7684\u6210\u5c31\u55ce\uff1f\u5728\u64c1\u6709\u4e86\u53ef\u4ee5\u4ee5\u5de5\u7a0b\u624b\u6cd5\u9020\u4eba\u7684\u5de5\u5177\u5f8c\uff0c\u4eba\u5011\u662f\u5426\u61c9\u8a72\u626e\u6f14\u4e0a\u5e1d\uff0c\u70ba\u6211\u5011\u7684\u57fa\u56e0\u5eab\u5e36\u4f86\u6c38\u6046\u7684\u6539\u8b8a\uff1f\u79d1\u5b78\u5bb6\u5011\u4e00\u5834\u5834\u8db3\u4ee5\u6539\u8b8a\u4eba\u985e\u6b77\u53f2\u7684\u7af6\u722d\u3001\u80cc\u53db\u5373\u5408\u4f5c\u5728\u4f5c\u8005\u83ef\u7279\u2022\u827e\u85a9\u514b\u68ee\u7b46\u4e0b\u751f\u52d5\u4e0a\u6f14\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #000000;\">\u6709\u5225\u65bc\u4e00\u822c\u79d1\u666e\u8b80\u7269\u7684\u751f\u786c\u7121\u8da3\uff0c\u66fe\u4efb\u6642\u4ee3\u96dc\u8a8c\u7e3d\u7de8\u8f2f\u7684\u83ef\u7279\u2022\u827e\u85a9\u514b\u68ee\u4ee5\u5c0f\u8aaa\u822c\u7684\u624b\u6cd5\uff0c\u5a13\u5a13\u9053\u51fa\u57fa\u56e0\u7de8\u8f2f\u5de5\u5177\u2014\u2014CRISPR\/cas9 \u7684\u767c\u5c55\u6b77\u7a0b\u3002\u4ed6\u5f9e\u672c\u66f8\u7684\u4e3b\u89d2\u2014\u767c\u5c55 CRISPR\/cas9 \u4e0d\u53ef\u6216\u7f3a\u7684\u95dc\u9375\u4eba\u7269\u2014\u73cd\u59ae\u4f5b\u2022\u9053\u7d0d\u5728\u7ae5\u5e74\u8207\u300a\u96d9\u87ba\u65cb\u300b\u7684\u7f8e\u9e97\u9082\u9005\u4e2d\u5de7\u5999\u7684\u5f15\u51fa\u4e86\u57fa\u56e0\u8207\u6f14\u5316\u907a\u50b3\u7684\u6b77\u53f2\uff0c\u6beb\u7121\u9055\u548c\u7684\u70ba\u8b80\u8005\u88dc\u8db3\u4e86\u76f8\u95dc\u80cc\u666f\u77e5\u8b58\uff0c\u63a5\u8457\u5f15\u9818\u8b80\u8005\u8ddf\u96a8\u8457\u9053\u7d0d\u6210\u9577\u7684\u8173\u6b65\uff0c\u8dcc\u8dcc\u649e\u649e\u7684\u9032\u5165\u4e86\u63a2\u7d22\u57fa\u56e0\u7684\u5927\u9580\u3002\u5728\u6210\u70ba\u79d1\u5b78\u5bb6\u7684\u65c5\u7a0b\u4e2d\uff0c\u9053\u7d0d\u8d70\u7684\u4e26\u4e0d\u8f15\u9b06\uff0c\u4e00\u8def\u4e0a\u4ed6\u66fe\u56f0\u60d1\u4e5f\u66fe\u53d7\u5230\u8cea\u7591\uff0c\u7136\u800c\u4e5f\u6709\u8a31\u591a\u4eba\u5e6b\u52a9\u4ed6\u4e00\u4e00\u514b\u670d\u56f0\u96e3\uff0c\u4e26\u6210\u70ba RNA\u9818\u57df\u7684\u6b0a\u5a01\u3002\u800c\u5728\u4e00\u4f4d\u5c08\u9580\u7814\u7a76 CRISPR\/cas9 \u7684\u79d1\u5b78\u5bb6\u57c3\u99ac\u7d10\u57c3\u723e\u00b7\u590f\u5f6d\u8482\u8036\u7684\u9080\u8acb\u4e0b\uff0c\u8eab\u70ba RNA \u5c08\u5bb6\u7684\u9053\u7d0d\u8de8\u9818\u57df\u7684\u958b\u59cb\u7814\u7a76\u57fa\u56e0\u7de8\u8f2f\u7684\u7cfb\u7d71\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #000000;\">\u57fa\u56e0\u7de8\u8f2f\u5de5\u5177 CRISPR\/Cas9 \u6700\u521d\u5176\u5be6\u662f\u4e00\u5957\u7d30\u83cc\u53e4\u8001\u7684\u9632\u79a6\u7cfb\u7d71\uff0c\u5176\u529f\u80fd\u70ba\u5c0d\u6297\u4ed6\u5011\u7684\u5929\u6575\u566c\u83cc\u9ad4(\u8a73\u898b\u5716\u4e00)\uff0c\u7576\u6642\u7684\u79d1\u5b78\u5bb6\u5df2\u7d93\u767c\u73fe\u4e86\u6b64\u7cfb\u7d71\u5927\u90e8\u5206\u7684\u5206\u5b50\u53ca\u5176\u529f\u80fd\uff0c\u537b\u59cb\u7d42\u7121\u6cd5\u5728\u8a66\u7ba1\u4e2d\u57f7\u884c\u6b64\u6a5f\u5236\u3002\u590f\u5f6d\u8482\u8036\u3001\u9053\u7d0d\u548c\u5176\u4ed6\u79d1\u5b78\u5bb6\u5728\u722d\u5148\u6050\u5f8c\u5730\u60f3\u8981\u6210\u70ba\u7b2c\u4e00\u7d44\u6210\u529f\u5728\u8a66\u7ba1\u4e2d\u6210\u529f\u526a\u63a5\u7684\u79d1\u5b78\u5bb6\uff0c\u4ed6\u5011\u5229\u7528\u5404\u5f0f\u5404\u6a23\u7684\u65b9\u6cd5\u60f3\u8981\u627e\u51fa\u95dc\u9375\u7684\u5206\u5b50\uff0c\u5728\u8cbb\u4e86\u4e00\u756a\u5de5\u592b\u5f8c\uff0c\u590f\u5f6d\u8482\u8036\u8207\u9053\u7d0d\u767c\u73fe\u4e86\u9019\u500b\u95dc\u9375\u5206\u5b50 tracrRNA \u4e26\u5c07 tracrRNA \u4ee5\u53ca\u53e6\u5916\u4e00\u6bb5\u540c\u6a23\u91cd\u8981\u7684 crRNA \u5408\u4f75\u6210\u4e86\u55ae\u4e00\u56ae\u5c0e RNA(\u5f15\u5c0e RNA)\uff0c\u4f7f\u9019\u5957\u7d30\u83cc\u7684\u7cfb\u7d71\u6210\u70ba\u4ee5\u500b\u53ef\u4ee5\u7528\u4f86\u526a\u8f2f\u57fa\u56e0\u7684\u5de5\u5177\u3002\u7136\u800c\u5728\u8a66\u7ba1\u4e2d\u57f7\u884c\u57fa\u56e0\u7de8\u8f2f\u4e26\u4e0d\u662f\u7af6\u8cfd\u7684\u7d42\u9ede\uff0c\u79d1\u5b78\u5bb6\u5011\u5e0c\u671b\u80fd\u5920\u5c07\u9019\u500b\u6e90\u65bc\u7d30\u83cc\u7684\u7cfb\u7d71\u61c9\u7528\u65bc\u7de8\u8f2f\u52d5\u7269\u57fa\u56e0\uff0c\u751a\u81f3\u662f\u7de8\u8f2f\u4eba\u985e\u57fa\u56e0\u3002\u9019\u500b\u5049\u5927\u7684\u76ee\u6a19\u5438\u5f15\u4e86\u66f4\u591a\u7684\u79d1\u5b78\u5bb6\u6295\u5165\u9019\u6230\u6cc1\u6fc0\u70c8\u7684\u6230\u5834\uff0c\u4f7f\u5f97\u6574\u500b\u79d1\u5b78\u754c\u7030\u6f2b\u8457\u4e00\u80a1\u706b\u85e5\u5473\uff0c\u5728\u4e0b\u534a\u5834\u7684\u7af6\u8cfd\u4e2d\uff0c\u53e6\u4e00\u4f4d\u79d1\u5b78\u5bb6\u2014\u5f35\u5cf0\u812b\u7a4e\u800c\u51fa\uff0c\u7387\u5148\u767c\u8868\u8ad6\u6587\u8aaa\u660e\u5982\u4f55\u4fee\u6539\u9019\u5957\u7cfb\u7d71\u4f7f\u4e4b\u80fd\u61c9\u7528\u65bc\u54fa\u4e73\u985e\uff0c\u4e5f\u5c31\u662f\u5ba3\u793a\u4e86 CRISPR\/Cas9\uff08\u8a73\u898b\u5716\u4e8c\uff09\u7684\u8a95\u751f\u3002\u4e0d\u904e\u4ed6\u7684\u52dd\u5229\u5145\u6eff\u722d\u8b70\uff0c\u5305\u62ec\u9053\u7d0d\u5728\u5167\u7684\u8a31\u591a\u79d1\u5b78\u5bb6\u8cea\u7591\u5f35\u5cf0\u5728\u7af6\u8cfd\u904e\u7a0b\u4e2d\u6240\u4f7f\u7528\u7684\u624b\u6bb5\uff0c\u540c\u6a23\u5730\u4e5f\u6709\u4e00\u7fa4\u79d1\u5b78\u5bb6\u64c1\u8b77\u5f35\u5cf0\uff0c\u5169\u65b9\u59cb\u7d42\u5404\u6301\u5df1\u898b\uff0c\u4e92\u4e0d\u76f8\u8b93\u3002\u4f46\u4e0d\u53ef\u5426\u8a8d\u7684\uff0c\u5728\u6539\u826f\u7d30\u83cc\u9632\u79a6\u7cfb\u7d71\uff0c\u4fc3\u6210\u9019\u500b\u50f9\u683c\u4f4e<\/span><span style=\"color: #000000;\">\u5ec9\u3001\u5bb9\u6613\u64cd\u4f5c\u7684\u57fa\u56e0\u7de8\u8f2f\u5de5\u5177 CRISPR\/Cas9 \u8a95\u751f\u7684\u904e\u7a0b\u4e2d\uff0c\u5f35\u5cf0\u4e5f\u662f\u4e00\u5927\u529f\u81e3\u3002<\/span><\/p>\n<p><span style=\"color: #000000;\">CRISPR\/Cas9 \u7684\u767c\u660e\u662f\u4eba\u985e\u6587\u660e\u7684\u4e00\u5927\u9032\u6b65\uff0c\u6211\u5011\u9996\u5ea6\u64c1\u6709\u4e86\u53ef\u4ee5\u6539\u5beb\u81ea\u5df1\u57fa\u56e0\u7684\u80fd\u529b\uff0c\u4f46\u96a8\u4e4b\u800c\u4f86\u7684\u662f\u9053\u5fb7\u65b9\u9762\u7684\u722d\u8b70\uff0c\u79d1\u5b78\u5bb6\u5011\u5c0d\u65bc\u4eba\u985e\u662f\u5426\u80fd\u5920\u57fa\u56e0\u7de8\u8f2f\u6211\u5011\u7684\u751f\u6b96\u7d30\u80de\u5c55\u958b\u4e86\u6fc0\u70c8\u7684\u8faf\u8ad6\uff0c\u6211\u5011\u7a76\u7adf\u662f\u5426\u6709\u6b0a\u5229\u53ef\u4ee5\u6539\u5beb\u5168\u9ad4\u4eba\u985e\u7684\u57fa\u56e0\u5eab\uff1f\u7576\u4eba\u985e\u80fd\u5920\u82b1\u9322\u6539\u5beb\u5f8c\u4ee3\u57fa\u56e0\u6642\uff0c\u662f\u5426\u6703\u9020\u6210\u8ca7\u5bcc\u5dee\u8ddd\u7684\u64f4\u5927\u4e26\u662f\u793e\u6703\u5e95\u5c64\u518d\u4e5f\u7121\u6cd5\u7ffb\u8eab\uff1f\u96e3\u9053\u5929\u64c7\u6703\u662f\u4e00\u500b\u6bd4\u4eba\u64c7\u66f4\u597d\u7684\u6f14\u5316\u65b9\u6cd5\u55ce\uff1f \u6211\u5011\u82e5\u662f\u80fd\u5920\u5c07\u67d0\u4e9b\u75be\u75c5\u7684\u57fa\u56e0\u5f9e\u5168\u4eba\u985e\u57fa\u56e0\u5eab\u4e2d\u5254\u9664\uff0c\u4f55\u6a02\u800c\u4e0d\u70ba\u5462\uff1f CRISPR\/Cas9 \u7684\u8a95\u751f\u7a76\u7adf\u662f\u4eba\u985e\u7684\u798f\u7949\u9084\u662f\u5e36\u4f86\u707d\u96e3\u7684\u6e90\u982d\uff1f\u4e00\u4ef6\u610f\u5916\u7684\u4e8b\u6545\u767c\u751f\uff0c\u5c0d\u5168\u4e16\u754c\u62cb\u51fa\u4e86\u9707\u64bc\u5f48\uff0c\u8feb\u4f7f\u5168\u4e16\u754c\u6bcf\u500b\u570b\u5bb6\u5fc5\u9808\u7acb\u523b\u9762\u5c0d\u9019\u500b\u5169\u96e3\u7684\u554f\u984c\u4e26\u5236\u5b9a\u51fa\u6cd5\u5f8b\u56e0\u61c9\u3002\u79d1\u5e7b\u5c0f\u8aaa\u4e2d\u4ee4\u4eba\u5bb3\u6015\u7684\u60c5\u7bc0\u771f\u7684\u6703\u5728\u73fe\u5be6\u4e0a\u6f14\u55ce\uff1f\u9084\u662f\u9019\u4e00\u5207\u90fd\u53ea\u662f\u675e\u4eba\u6182\u5929\u5462\uff1f\u6b63\u5982\u540c\u9053\u7d0d\u6240\u8aaa\u7684\u6211\u5011\u5fc5\u9808\u627e\u51fa\u4e00\u689d\u300c\u8b39\u614e\u7684\u524d\u9032\u9053\u8def\u300d\uff0c\u4e0d\u80fd\u56e0\u70ba\u6050\u61fc\u800c\u7981\u6b62\u9019\u9805\u79d1\u6280\u7684\u767c\u5c55\uff0c\u7136\u800c\u6211\u5011\u4e5f\u4e0d\u80fd\u5728\u672a\u7d93\u751f\u601d\u719f\u616e\u7684\u72c0\u614b\u4e0b\u505a\u51fa\u83bd\u649e\u7684\u8209\u52d5\u3002\u300a\u7834\u89e3\u57fa\u56e0\u78bc\u7684\u4eba\u300b\u63d0\u4f9b\u8b80\u8005\u4e00\u5834\u79d1\u5b78\u8207\u4eba\u6027\u7684\u5947\u5e7b\u65c5\u7a0b\uff0c\u4eba\u985e\u63a2\u7d22\u672a\u77e5\u7684\u71b1\u60c5\u5f9e\u4f5c\u8005\u7b46\u5c16\u6e90\u6e90\u4e0d\u65b7\u6d41\u51fa\uff0c\u5f15\u9818\u8b80\u8005\u96a8\u8457\u66f8\u4e2d\u4eba\u7269\u3001\u60c5\u7bc0\u4e00\u9801\u9801\u95d6\u95dc\uff0c\u4e00\u540c\u5c0b\u627e\u672a\u77e5\u7684\u89e3\u7b54\uff01<\/span><\/p>\n<p><strong><span style=\"color: #000000;\">\u4f5c\u54c1\u8a55\u8a9e<\/span><\/strong><br \/>\n<span style=\"color: #000000;\">\u908f\u8f2f\u6e05\u695a\uff0c\u5c64\u6b21\u5206\u660e\uff0c\u78ba\u5be6\u638c\u63e1\u91cd\u9ede\uff0c\u53ef\u898b\u5c0d\u8a72\u66f8\u6709\u5145\u5206\u4e86\u89e3\uff0c\u4e14\u6558\u8ff0\u624b\u6cd5\u5177\u5438\u5f15\u529b\uff0c\u662f\u7d50\u69cb\u5b8c\u6574\u7684\u4f73\u4f5c\uff01<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #000000;\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone size-medium wp-image-5385\" src=\"https:\/\/case.ntu.edu.tw\/scinarrator\/wp-content\/uploads\/2023\/03\/\u7834\u89e3\u57fa\u56e0\u78bc\u7684\u4eba.jpg\" alt=\"\" width=\"300\" height=\"300\" \/><\/span><\/p>\n<p><span style=\"color: #000000;\">\u66f8\u540d\uff1a\u7834\u89e3\u57fa\u56e0\u78bc\u7684\u4eba\uff1a\u8afe\u8c9d\u723e\u734e\u5f97\u4e3b\u73cd\u59ae\u4f5b\uff0e\u9053\u7d0d\u3001\u57fa\u56e0\u7de8\u8f2f\uff0c\u4ee5\u53ca\u4eba\u985e\u7684\u672a\u4f86<\/span><br \/>\n<span style=\"color: #000000;\">\u4f5c\u8005\uff1a\u83ef\u7279\uff0e\u827e\u85a9\u514b\u68ee\uff08Walter Isaacson\uff09<\/span><br \/>\n<span style=\"color: #000000;\">\u8b6f\u8005\uff1a\u9ea5\u6167\u82ac<\/span><br \/>\n<span style=\"color: #000000;\">\u51fa\u7248\u793e\uff1a\u5546\u5468\u51fa\u7248<\/span><br \/>\n<span style=\"color: #000000;\">\u51fa\u7248\u5e74\uff1a2021<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u64b0\u6587\uff1a\u8521\u4f69\u747e\uff5c\u5ef6\u5e73\u9ad8\u4e2d \u57fa\u56e0\u7de8\u8f2f\u7684\u5947\u5e7b\u65c5\u7a0b\uff1a\u8b80\u300a\u7834\u89e3\u57fa\u56e0\u78bc\u7684 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