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【臺灣大學】 99-B1-2 奈米磁顆粒在生醫治療之應用研究

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申請學校:臺灣大學

計畫編號:99-B1-2

計畫名稱:奈米磁顆粒在生醫治療之應用研究

領域別:物理-醫學

主持人:張富雄

系所:生物化學暨分子生物學研究所

奈米科技之生物醫學運用已是一個新興的研究領域。數種新穎性的奈米粒子,因其設計組合形式不同,能產生特殊物理化學性質,已可作為生物醫學之有效奈米探針,尤其在體外的功能檢測已有成功之應用。因此本計畫將運用奈米磁顆粒之磁熱轉換的特性,應用於醫學治療的可行性,該項研究涉及基礎物理及生醫知識技術的整合,可藉由本計畫之執行,加強醫學系學生從事跨領域之實驗研究經驗。首先,在奈米磁顆粒表面利用生物分子作功能性修飾,使奈米材料具有生物相容性,並同時具有導向遞送的能力。而在粒子表面修飾的方法中,讓脂質自發性地聚積於粒子表面,成為脂質膜包覆的形式為最快速有效的方式。本研究將使用奈米磁性氧化鐵,以化學共沉法合成,控制實驗pH值,可以得磁顆粒粒徑約為十至二十奈米之大小,隨著磁性標記奈米科技的製作經驗提升,將可製造出可調控粒徑大小的磁性奈米粒子,方能產生有效之磁熱效應。這些氧化鐵奈米磁顆粒再經過生物技術來製作表面修飾有生物分子的奈米粒子,例如將其表面修飾正價或PEG來增加水溶性,降低其在生體內的聚集和清除,並能減少其對細胞組織的毒性。這些奈米磁顆粒可經由生物探針(如抗體或配位體)的結合被癌細胞所吞噬,並藉由外界的磁場的交互作用在細胞或生物體內產生熱效應,以達到熱炙治療之功效。

關鍵字:奈米磁顆粒、表面修飾、熱炙治療

Abstract

The application of nanotechnology in biomedicine has been extensively developed in recent years. Various forms of nanoparticles with distinct properties can act as novel nanoprobes for diversely functional applications.? However, to design a nanomaterial with good biocompatibility for targeted imaging or therapy in living subject is still a challenging issue. With the increasing importance of magnetically labeled nanoparticles in clinical applications, the study on the synthesis of controllable sizes of magnetic nanoparticles may play a role in promoting accuracy of targeted therapy. In this proposal for medical students, iron oxide nanoparticles are going to be coat with the suitable lipid ingredients for solubility and tissue compatibility. To further facilitate targeted moieties in nanoparticles with iron oxide cores, we propose to use functional lipids (e.g. ligand- or antibody-linked lipids) that can be post-inserted into the magnetically lipid-coated nanoparticles.? The conjugation technology can be preformed by direct chemical modification or by a layer-by-layer coating method. It will be the first time for medical students to assemble such nanoparticles with adaptor proteins (such as streptavidin) for targeting. In the experiments, student can learn how to perform a complex experiment using component materials and stepwise procedure. They will learn some basic principles in biology, chemistry and physics. In summary, we proposed a lipid-coating method to encapsulate magnetic iron oxide nanoparticles.? It will serve as bimodal (i.e. optical and MRI) contrast agents, and also used for hyperthermia application.?

Key Words:magnetic nanoparticles、surface modification、hyperthermia?

奈米科技之生物醫學運用已是一個新興的研究領域。數種新穎性的奈米粒子,因其設計組合形式不同,能產生特殊物理化學性質,已可作為生物醫學之有效奈米探針,尤其在體外的功能檢測已有成功之應用。因此本計畫將運用奈米磁顆粒之磁熱轉換的特性,應用於醫學治療的可行性,該項研究涉及基礎物理及生醫知識技術的整合,可藉由本計畫之執行,加強醫學系學生從事跨領域之實驗研究經驗。首先,在奈米磁顆粒表面利用生物分子作功能性修飾,使奈米材料具有生物相容性,並同時具有導向遞送的能力。而在粒子表面修飾的方法中,讓脂質自發性地聚積於粒子表面,成為脂質膜包覆的形式為最快速有效的方式。本研究將使用奈米磁性氧化鐵以化學共沉法合成,控制實驗pH值,可以得磁顆粒粒徑約為十至二十奈米之大小隨著磁性標記奈米科技的製作經驗提升,將可製造出可調控粒徑大小的磁性奈米粒子,方能產生有效之磁熱效應。這些氧化鐵奈米磁顆粒再經過生物技術來製作表面修飾有生物分子的奈米粒子,例如將其表面修飾正價或PEG來增加水溶性,降低其在生體內的聚集和清除,並能減少其對細胞組織的毒性。這些奈米磁顆粒可經由生物探針(如抗體或配位體)的結合被癌細胞所吞噬,並藉由外界的磁場的交互作用在細胞或生物體內產生熱效應,以達到熱炙治療之功效。

關鍵字:奈米磁顆粒、表面修飾、熱炙治療

?

Abstract

The application of nanotechnology in biomedicine has been extensively developed in recent years. Various forms of nanoparticles with distinct properties can act as novel nanoprobes for diversely functional applications.? However, to design a nanomaterial with good biocompatibility for targeted imaging or therapy in living subject is still a challenging issue. With the increasing importance of magnetically labeled nanoparticles in clinical applications, the study on the synthesis of controllable sizes of magnetic nanoparticles may play a role in promoting accuracy of targeted therapy. In this proposal for medical students, iron oxide nanoparticles are going to be coat with the suitable lipid ingredients for solubility and tissue compatibility. To further facilitate targeted moieties in nanoparticles with iron oxide cores, we propose to use functional lipids (e.g. ligand- or antibody-linked lipids) that can be post-inserted into the magnetically lipid-coated nanoparticles.? The conjugation technology can be preformed by direct chemical modification or by a layer-by-layer coating method. It will be the first time for medical students to assemble such nanoparticles with adaptor proteins (such as streptavidin) for targeting. In the experiments, student can learn how to perform a complex experiment using component materials and stepwise procedure. They will learn some basic principles in biology, chemistry and physics. In summary, we proposed a lipid-coating method to encapsulate magnetic iron oxide nanoparticles.? It will serve as bimodal (i.e. optical and MRI) contrast agents, and also used for hyperthermia application.?

?

Key Wordsmagnetic nanoparticlessurface modificationhyperthermia

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