{"id":9297,"date":"2010-09-29T03:27:24","date_gmt":"2010-09-28T19:27:24","guid":{"rendered":"http:\/\/highscope.ch.ntu.edu.tw\/wordpress\/?p=9297"},"modified":"2021-10-06T16:31:32","modified_gmt":"2021-10-06T08:31:32","slug":"rna%e8%81%9a%e5%90%88%e9%85%b6%ef%bc%88rna-polymerase%ef%bc%89%ef%bc%88ii%ef%bc%89","status":"publish","type":"post","link":"http:\/\/localhost\/rna%e8%81%9a%e5%90%88%e9%85%b6%ef%bc%88rna-polymerase%ef%bc%89%ef%bc%88ii%ef%bc%89\/","title":{"rendered":"RNA\u805a\u5408\u9176\uff08RNA Polymerase\uff09\uff08II\uff09"},"content":{"rendered":"

RNA\u805a\u5408\u9176\uff08RNA Polymerase\uff09\uff08II\uff09<\/span><\/strong>
\n\u570b\u7acb\u65b0\u838a\u9ad8\u7d1a\u4e2d\u5b78\u9673\u5049\u6c11\u9000\u4f11\u6559\u5e2b\/\u570b\u7acb\u53f0\u7063\u5e2b\u7bc4\u5927\u5b78\u5316\u5b78\u7cfb\u8449\u540d\u5009\u6559\u6388\u8cac\u4efb\u7de8\u8f2f
\n<\/strong><\/span>
\nRNA\u805a\u5408\u9176\u7684\u4f5c\u7528<\/strong>
\n\u7d50\u5408\u8207\u8d77\u59cb\u671f<\/strong>
\n\u5728\u539f\u6838\u751f\u7269\u4e2d\uff0cRNA\u805a\u5408\u9176\u7684\u7d50\u5408\uff0c\u5305\u542b\u03b1\u6b21\u55ae\u5143\u8fa8\u8a8d\u51faDNA\u7684\u4e0a\u6e38\u55ae\u5143\uff08upstream element\uff09\uff0840\u81f3-70\u9e7c\u57fa\u5c0d\uff09\uff0c\u4ee5\u53ca\u03c3\u56e0\u5b50\u8fa8\u8a8d\u51fa-10\u81f3-35\u5340\u584a\u3002\u6709\u6578\u500b\u03c3\u56e0\u5b50\u8abf\u63a7\u57fa\u56e0\u8868\u73fe\u3002\u8209\u4f8b\u800c\u8a00\uff0c\u5728\u6b63\u5e38\u689d\u4ef6\u4e0b\uff0c\u03c370\u6703\u8868\u73fe\uff0c\u4f7fRNAP\u8207\u7ba1\u5bb6\u57fa\u56e0\u7d50\u5408\uff0c\u800c\u03c332\u8a98\u4f7fRNAP\u8207\u71b1\u4f11\u514b\u57fa\u56e0\u7d50\u5408\u3002<\/p>\n

\u8207DNA\u7d50\u5408\u4e4b\u5f8c\uff0cRNA\u805a\u5408\u9176\u7531\u9589\u5408\u8907\u5408\u9ad4\u5207\u63db\u6210\u958b\u653e\u8907\u5408\u9ad4\u3002\u6b64\u4e00\u8b8a\u5316\u5305\u62ecDNA\u5169\u80a1\u5206\u96e2\uff0c\u5f62\u6210\u89e3\u87ba\u65cb\u7684DNA\u5340\u6bb5\uff0c\u9577\u5ea6\u7d04\u670913 bp\uff08\u9e7c\u57fa\u5c0d\u4e4b\u7e2e\u5beb\uff09\u3002\u6839\u64da\u83ef\u751f\uff0d\u514b\u91cc\u514b\u9e7c\u57fa\u5c0d\u4ea4\u4e92\u4f5c\u7528\uff0c\u6838\u82f7\u9178\u548c\u6a21\u677fDNA\u80a1\u4e4b\u9593\u4ee5\u9e7c\u57fa\u914d\u5c0d\u3002\u56e0\u70baDNA\u8981\u89e3\u65cb\u53ca\u91cd\u65cb\uff0c\u6240\u4ee5\u8d85\u7dda\u5708\uff08supercoiling\uff09\u5728\u805a\u5408\u9176\u7684\u6d3b\u6027\u4e2d\uff0c\u5360\u6709\u91cd\u8981\u5730\u4f4d\u3002\u5728RNAP\u524d\u65b9\u7684DNA\u5340\u6bb5\u8981\u89e3\u65cb\uff0c\u6709\u4e00\u88dc\u511f\u6027\u6b63\u8d85\u7dda\u5708\u5b58\u5728\u3002\u5728RNAP\u5f8c\u65b9\u7684DNA\u5340\u6bb5\u8981\u91cd\u65cb\uff0c\u5b58\u5728\u8457\u8ca0\u8d85\u7dda\u5708\u3002<\/p>\n

\u5ef6\u9577\u671f<\/strong>
\n\u8f49\u9304\u5ef6\u9577\u671f\u5305\u542b\u9032\u4e00\u6b65\u52a0\u5165\u6838\u7cd6\u6838\u82f7\u9178\uff0c\u4ee5\u53ca\u958b\u653e\u5f0f\u8907\u5408\u9ad4\u8b8a\u6210\u8f49\u9304\u8907\u5408\u9ad4\u3002\u56e0\u70baRNAP\u8207\u555f\u52d5\u5b50\u7684\u7d50\u5408\u5f88\u5f37\uff0c\u7121\u6cd5\u958b\u59cb\u5f62\u6210\u5b8c\u6574\u9577\u5ea6\u7684\u8f49\u9304\u672c\u3002\u6b64\u4e00\u968e\u6bb5\u7684\u8f49\u9304\uff0c\u751f\u6210\u8f03\u77ed\u7684RNA\u7247\u6bb5\uff0c\u5927\u7d04\u53ea\u67099 bp\uff0c\u6b64\u4e00\u6b65\u9a5f\u7a31\u70ba\u9000\u5316\u8f49\u9304\u3002\u4e00\u65e6RNAP\u958b\u59cb\u5f62\u6210\u8f03\u9577\u7684\u8f49\u9304\u672c\uff0c\u5b83\u6703\u6e05\u9664\u555f\u52d5\u5b50\u3002\u6b64\u6642\uff0c-10\u81f3-35\u555f\u52d5\u5b50\u5340\u584a\u74e6\u89e3\uff0c\u03c3\u56e0\u5b50\u812b\u96e2RNAP\u3002\u7576\u03c3\u56e0\u5b50\u6293\u7dcaRNAP\u7684\u9069\u7576\u4f4d\u7f6e\u6642\uff0c\u5269\u9918\u7684RNAP\u8907\u5408\u9ad4\u5411\u524d\u79fb\u52d5\u300217-bp\u8f49\u9304\u8907\u5408\u9ad4\u67098-bp\u7684DNA-RNA\u6df7\u6210\u9ad4\uff0c\u4e5f\u5c31\u662f\u8aaa\uff0c\u67098\u500b\u9e7c\u57fa\u5c0d\u53c3\u8207RNA\u8f49\u9304\u672c\u8207DNA\u6a21\u677f\u80a1\u7684\u7d50\u5408\u3002\u7576\u9032\u884c\u8f49\u9304\u6642\uff0c\u6838\u7cd6\u6838\u82f7\u9178\u7531RNA\u8f49\u9304\u672c\u76843\u2019\u7aef\u52a0\u5165\uff0c\u800c\u4e14RNAP\u8907\u5408\u9ad4\u6cbf\u8457DNA\u79fb\u52d5\u3002\u96d6\u7136RNAP\u770b\u8d77\u4f86\u6c92\u67093\u2019\u672b\u7aef\u6838\u9178\u9176\uff08exonuclease\uff09\u7684\u6d3b\u6027\uff0c\u4e0d\u6703\u50cfDNA\u805a\u5408\u9176\u90a3\u6a23\u6709\u6821\u5c0d\u7684\u529f\u80fd\uff0c\u4f46\u6709\u8b49\u64da\u986f\u793a\uff0cRNAP\u9047\u5230\u914d\u5c0d\u932f\u8aa4\u7684\u9e7c\u57fa\u5c0d\u6703\u66ab\u505c\uff0c\u4e26\u5c07\u932f\u8aa4\u66f4\u6b63\u3002<\/p>\n

\u628a\u6838\u7cd6\u6838\u82f7\u9178\u52a0\u5165RNA\u8f49\u9304\u672c\u4e2d\uff0c\u5176\u6a5f\u5236\u8207DNA\u805a\u5408\u9176\u975e\u5e38\u985e\u4f3c\u2500\u2500\u4e00\u822c\u76f8\u4fe1\uff0c\u9019\u4e9b\u805a\u5408\u9176\u5728\u6f14\u5316\u4e0a\u6709\u76f8\u95dc\u6027\u3002PNAP\u7684\u5929\u51ac\u80fa\uff08asp\uff09\u6b98\u682a\u6703\u7dca\u7dca\u6293\u4f4fMg2+<\/sup>\u96e2\u5b50\uff0c\u53cd\u904e\u4f86\uff0cMg2+<\/sup>\u96e2\u5b50\u8207\u6838\u7cd6\u6838\u82f7\u9178\u7684\u78f7\u9178\u6839\u5f62\u6210\u914d\u4f4d\u9375\u7d50\u3002\u7b2c\u4e00\u500bMg2+<\/sup>\u6703\u6293\u7dca\u8981\u52a0\u5165\u7684NTP\u4e4b\u03b1\u78f7\u9178\u6839\u3002\u4f7f\u5f97RNA\u8f49\u9304\u672c\u5c0d3\u2019OH\u9032\u884c\u89aa\u6838\u653b\u64ca\uff0c\u5c07\u65b0\u7684NTP\u52a0\u5165\u93c8\u4e2d\u3002\u7b2c\u4e8c\u500bMg2+<\/sup>\u6703\u6293\u7dcaNTP\u7684\u7126\u78f7\u9178\u6839\uff0c\u7e3d\u53cd\u61c9\u65b9\u7a0b\u5f0f\u70ba\uff1a
\n(NMP)n<\/sub> + NTP –> (NMP)n+1<\/sub> + PPi<\/sub><\/p>\n

\u7d42\u7d50\u671f<\/strong>
\nRNA\u8f49\u9304\u7684\u7d42\u7d50\u5206\u70baRho-\u7368\u7acb\u53caRho-\u4f9d\u8cf4\u5169\u7a2e\u3002
\n\u672a\u63a5\u53d7rho \u86cb\u767d\u8cea\u7684\u8f49\u9304\u7d42\u7d50\uff0c\u70baRho-\u7368\u7acb\u8f49\u9304\u7d42\u7d50\u3002DNA\u7684\u91cd\u8907\u5340\u6bb5\u5728\u8f49\u9304\u6642\uff0c\u9020\u6210RNA\u8f49\u9304\u8ff4\u5708\u5f62\u6210\u9aee\u593e\u8ff4\u7d0b\u7d50\u69cb\uff0c\u4e14\u540c\u4e00\u80a1\u4e0d\u540c\u5340\u6bb5\u4e4b\u9593\u4ee5\u9e7c\u57fa\u5c0d\u9023\u63a5\u3002\u9019\u7a2e\u7d50\u69cb\u901a\u5e38\u5bcc\u542bG-C\u9e7c\u57fa\u5c0d\uff0c\u4f7f\u5176\u6bd4DNA-RNA\u6df7\u6210\u9ad4\u66f4\u7a69\u5b9a\u3002\u7d50\u679c\uff0c\u5728\u8f49\u9304\u8907\u5408\u7269\uff0c8 bp\u7684DNA-RNA\u6df7\u6210\u9ad4\u8b8a\u62104 bp\u6df7\u6210\u9ad4\u3002\u6700\u5f8c4\u9e7c\u57fa\u5c0d\u662f\u8f03\u5f31\u7684A-U\u9e7c\u57fa\u5c0d\uff0c\u6574\u500bRNA\u8f49\u9304\u672c\u6703\u8207DNA\u812b\u96e2\u3002<\/p>\n

\u53c3\u8003\u8cc7\u6599\uff1a<\/strong>
\nhttp:\/\/en.wikipedia.org\/wiki\/RNA_polymerase<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

RNA\u805a\u5408\u9176\uff08RNA Polymerase\uff09\uff08II\uff09 \u570b\u7acb\u65b0\u838a\u9ad8\u7d1a\u4e2d\u5b78\u9673\u5049\u6c11\u9000\u4f11\u6559\u5e2b\/\u570b\u7acb\u53f0\u7063\u5e2b\u7bc4\u5927\u5b78\u5316\u5b78\u7cfb\u8449…<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108,78,77],"tags":[1293,1292,1247],"class_list":["post-9297","post","type-post","status-publish","format-standard","hentry","category-chemistry00","category-chemistry08-01","category-chemistry08","tag-rna-polymerase","tag-rna","tag-1247","loop-entry","cat-108","cat-78","cat-77","no-thumbnail"],"views":9834,"_links":{"self":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/9297","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/comments?post=9297"}],"version-history":[{"count":1,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/9297\/revisions"}],"predecessor-version":[{"id":89545,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/9297\/revisions\/89545"}],"wp:attachment":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/media?parent=9297"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/categories?post=9297"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/tags?post=9297"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}