{"id":7166,"date":"2010-09-10T07:38:16","date_gmt":"2010-09-09T23:38:16","guid":{"rendered":"http:\/\/highscope.ch.ntu.edu.tw\/wordpress\/?p=7166"},"modified":"2021-10-06T16:32:17","modified_gmt":"2021-10-06T08:32:17","slug":"%e5%96%ae%e6%a0%aa%e6%8a%97%e9%ab%94%ef%bc%88monoclonal-antibody%ef%bc%89-%e4%b8%8b","status":"publish","type":"post","link":"http:\/\/localhost\/%e5%96%ae%e6%a0%aa%e6%8a%97%e9%ab%94%ef%bc%88monoclonal-antibody%ef%bc%89-%e4%b8%8b\/","title":{"rendered":"\u55ae\u682a\u6297\u9ad4\uff08Monoclonal Antibody\uff09-\u4e0b"},"content":{"rendered":"

\u55ae\u682a\u6297\u9ad4\uff08Monoclonal Antibody\uff09-\u4e0b<\/span>
\n\u570b\u7acb\u5609\u7fa9\u9ad8\u7d1a\u5546\u696d\u8077\u696d\u5b78\u6821\u751f\u7269\u79d1\u88d8\u6587\u99a5\u8001\u5e2b\/\u570b\u7acb\u53f0\u7063\u5e2b\u7bc4\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u674e\u51a0\u7fa4\u52a9\u7406\u6559\u6388\u8cac\u4efb\u7de8\u8f2f<\/span><\/strong><\/p>\n

\uff08\u4e00\uff09\u55ae\u682a\u6297\u9ad4\u7684\u88fd\u4f5c
\n\u3010\u8a3b\u3011\u73fe\u5728\u5e38\u7528\u7684\u9aa8\u9ad3\u7624\u7d30\u80de\u662f\u5c0f\u767d\u9f20BALB\/c\u764c\u7d30\u80de\u682a\uff08NS-1\uff09\uff0c\u4f46\u5176\u9ad4\u5167\u7f3a\u4e4f\u4e8c\u7a2e\u8207\u6838\u9178\u4ee3\u8b1d\u6709\u95dc\u7684\u9175\u7d20\uff08thymidine kinase \u53cahypoxanthine-guanine phosphoribosyl transferase\uff09\uff0c\u82e5\u5c07\u5b83\u57f9\u990a\u5728HAT\u57f9\u990a\u57fa\uff08\u5167\u542baminopterin\u3001thymidine \u53ca hypoxanthine\uff09\u6642\uff0c\u56e0\u6838\u9178\u7121\u6cd5\u6b63\u5e38\u4ee3\u8b1d\u800c\u4e0d\u80fd\u751f\u9577\u3002\u800c\u4e00\u822c\u6b63\u5e38\u7d30\u80de\uff0c\u5982B\u7d30\u80de\uff0c\u5247\u80fd\u751f\u9577\u5728HAT\u57f9\u990a\u57fa\u4e0a\uff0c\u4f46\u96a8\u8457\u5176\u751f\u547d\u5230\u9054\u4e5f\u6703\u6b7b\u4ea1\u3002\u56e0\u6b64\u552f\u6709\u540c\u6642\u5177\u6709B\u7d30\u80de\u548c\u764c\u7d30\u80de\u7684\u878d\u5408\u7624\u624d\u80fd\u5728HAT\u57f9\u990a\u57fa\u4e0a\u4e0d\u65b7\u5730\u751f\u9577\u3002<\/p>\n

\uff08\u4e8c\uff09\u55ae\u682a\u6297\u9ad4\u7684\u512a\u9ede\u53ca\u61c9\u7528
\n\u55ae\u682a\u6297\u9ad4\u5177\u6709\u6975\u9ad8\u7684\u5c08\u4e00\u6027\uff08specificity\uff09\uff0c\u53ea\u6703\u8207\u55ae\u4e00\u7684\u6297\u539f\u6c7a\u5b9a\u57fa\u7d50\u5408\uff0c\u4e0d\u6703\u96a8\u610f\u7d50\u5408\u5176\u4ed6\u90e8\u4f4d\u3002\u5176\u4ea6\u5177\u6709\u9ad8\u5ea6\u7684\u654f\u611f\u6027\uff08sensitivity\uff09\uff0c\u6240\u4ee5\u53ef\u4ee5\u81ea\u7531\u63a7\u5236\u55ae\u682a\u6297\u9ad4\u7684\u6fc3\u5ea6\uff0c\u4ee5\u4f5c\u70ba\u6aa2\u9a57\u7684\u5de5\u5177\u3002\u76ee\u524d\u55ae\u682a\u6297\u9ad4\u5df2\u5ee3\u6cdb\u5730\u61c9\u7528\u65bc\u91ab\u5b78\u3001\u5de5\u696d\u3001\u8fb2\u7267\u696d\u7b49\u4e0d\u540c\u9818\u57df\u4e0a\u3002<\/p>\n

1.\u5728\u91ab\u5b78\u6cbb\u7642\u4e0a\u4e4b\u61c9\u7528\uff1a\u56e0\u55ae\u682a\u6297\u9ad4\u5177\u6709\u5c08\u4e00\u6027\uff0c\u6545\u53ef\u5728\u55ae\u682a\u6297\u9ad4\u4e0a\u7d50\u5408\u4e0d\u540c\u7684\u7269\u8cea\uff0c\u5982\u63a5\u4e0a\u653e\u5c04\u6027\u7269\u8cea\u3001\u9175\u7d20\u3001\u87a2\u5149\u5291\u7b49\uff0c\u5247\u53ef\u4f5c\u70ba\u91ab\u5b78\u6aa2\u9a57\u8a66\u5291\uff0c\u53ef\u6e2c\u5f97\u9ad4\u5167\u6297\u539f\u4e4b\u6578\u91cf\u3002\u6216\u662f\u63a5\u4e0a\u85e5\u7269\uff0c\u5373\u53ef\u5c07\u85e5\u7269\u5e36\u5230\u67d0\u4e9b\u7279\u5b9a\u7d30\u80de\uff08\u5982\u764c\u7d30\u80de\u7b49\uff09\u4f5c\u7528\uff0c\u800c\u4e0d\u6703\u5f71\u97ff\u5230\u6b63\u5e38\u7d30\u80de\u3002<\/p>\n

2.\u5728\u5de5\u696d\u7d14\u5316\u4e4b\u61c9\u7528\uff1a\u5229\u7528\u55ae\u682a\u6297\u9ad4\u8207\u81a0\u9ad4\u57fa\u8cea\uff08gel matrix\uff09\u7d50\u5408\uff0c\u53ef\u4f5c\u70ba\u5c08\u4e00\u6027\u89aa\u548c\u6027\u7ba1\u67f1\uff0c\u53ef\u7528\u4ee5\u7d14\u5316\u4e26\u6fc3\u7e2e\u6709\u7528\u7269\u8cea\u3002<\/p>\n

3.\u5728\u8fb2\u7267\u696d\u4e0a\u4e4b\u61c9\u7528\uff1a\u5728\u8fb2\u696d\u4e0a\uff0c\u53ef\u61c9\u7528\u65bc\u767c\u5c55\u7121\u75c5\u6bd2\u690d\u7269\uff08virus-free plant\uff09\uff0c\u6216\u6aa2\u9a57\u75c5\u539f\u9ad4\u7b49\u3002\u5728\u755c\u7267\u696d\u4e0a\uff0c\u53ef\u7528\u4f5c\u6aa2\u9a57\u75c5\u539f\u9ad4\uff0c\u4ea6\u80fd\u767c\u5c55\u6210\u52d5\u7269\u75ab\u82d7\u3002<\/p>\n

4.\u5728\u7814\u7a76\u5de5\u4f5c\u4e0a\u4e4b\u61c9\u7528\uff1a\u7814\u7a76\u904e\u7a0b\u4e2d\u9047\u5230\u672a\u77e5\u6216\u7121\u6cd5\u7d14\u5316\u7684\u7269\u8cea\u6642\uff0c\u53ef\u5229\u7528\u55ae\u682a\u6297\u9ad4\u5354\u52a9\u5206\u96e2\u3001\u7d14\u5316\u3002<\/p>\n

\u8acb\u53c3\u95b1\u55ae\u682a\u6297\u9ad4\uff08Monoclonal Antibody\uff09-\u4e0a<\/a><\/p>\n

\u53c3\u8003\u8cc7\u6599
\n1.Ezzell C (2001) Magic Bullets Fly Again. Scientific American 285, pp28~35
\n2.Richard A. Goldsby et al (2000) Kuby Immunology (4e) http:\/\/www.whfreeman.com\/kuby\/<\/a>
\n3.\u55ae\u682a\u6297\u9ad4\u3002http:\/\/juang.bst.ntu.edu.tw\/ECX\/monoclonal.htm<\/a>\u3002<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

\u55ae\u682a\u6297\u9ad4\uff08Monoclonal Antibody\uff09-\u4e0b \u570b\u7acb\u5609\u7fa9\u9ad8\u7d1a\u5546\u696d\u8077\u696d\u5b78\u6821\u751f\u7269\u79d1\u88d8\u6587\u99a5\u8001\u5e2b\/\u570b\u7acb\u53f0\u7063\u5e2b\u7bc4…<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[110,4815,1166],"tags":[5132],"class_list":["post-7166","post","type-post","status-publish","format-standard","hentry","category-lifesciecne00","category-lifesciecne006","category-lifesciecne006-05","tag-5132","loop-entry","cat-110","cat-4815","cat-1166","no-thumbnail"],"views":13278,"_links":{"self":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/7166","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/comments?post=7166"}],"version-history":[{"count":1,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/7166\/revisions"}],"predecessor-version":[{"id":89752,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/7166\/revisions\/89752"}],"wp:attachment":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/media?parent=7166"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/categories?post=7166"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/tags?post=7166"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}