{"id":64744,"date":"2015-08-26T10:34:19","date_gmt":"2015-08-26T02:34:19","guid":{"rendered":"http:\/\/highscope.ch.ntu.edu.tw\/wordpress\/?p=64744"},"modified":"2021-10-06T16:10:46","modified_gmt":"2021-10-06T08:10:46","slug":"%e8%a6%aa%e5%92%8c%e5%b1%a4%e6%9e%90","status":"publish","type":"post","link":"http:\/\/localhost\/%e8%a6%aa%e5%92%8c%e5%b1%a4%e6%9e%90\/","title":{"rendered":"\u89aa\u548c\u5c64\u6790"},"content":{"rendered":"

\u89aa\u548c\u5c64\u6790 (Affinity chromatography)<\/strong><\/span>
\n\u570b\u7acb\u81fa\u7063\u5e2b\u7bc4\u5927\u5b78\u5316\u5b78\u7cfb\u7814\u7a76\u751f \u9673\u57f9\u6770<\/strong><\/span><\/p>\n

\u4e00\u822c\u7684\u7ba1\u67f1\u5c64\u6790(Column chromatography)\u53ef\u5206\u70ba\u6c23\u76f8\u5c64\u6790(Gas chromatography)\u548c\u6db2\u76f8\u5c64\u6790(Liquid chromatography)\uff0c\u5982\u679c\u8981\u518d\u7d30\u5206\uff0c\u53ef\u5206\u70ba\u6c23\u6db2\u76f8\u5c64\u6790\u3001\u6c23\u56fa\u76f8\u5c64\u6790\u2026\u2026\u7b49\uff0c\u9084\u6709\u5e7e\u7a2e\u6bd4\u8f03\u7279\u5225\u7684\u5c64\u6790\u65b9\u6cd5\uff0c\u50cf\u662f\u5229\u7528\u96e2\u5b50\u4ea4\u63db\u5c64\u6790\u6cd5\u6216\u8005\u5229\u7528\u662f\u5b54\u5f91\u5927\u5c0f\u4e0d\u540c\u9054\u5230\u5206\u96e2\u6548\u679c\u7684\u5c64\u6790\uff0c\u672c\u7bc7\u8981\u4ecb\u7d39\u7684\u89aa\u548c\u5c64\u6790\u4e5f\u662f\u6bd4\u8f03\u7279\u6b8a\u985e\u7684\u5c64\u6790\u3002<\/p>\n

\u89aa\u548c\u5c64\u6790\u4e3b\u8981\u61c9\u7528\u65bc\u751f\u7269\u5927\u5206\u5b50\u7684\u7d14\u5316\uff0c\u5229\u7528\u751f\u7269\u5927\u5206\u5b50\u4f86\u8ddf\u914d\u9ad4\u4e4b\u9593\u5f62\u6210\u53ef\u9006\u7d50\u5408\u7684\u80fd\u529b\uff0c\u4f7f\u5176\u5206\u96e2\u3002\u5176\u539f\u7406\u50cf\u662f\u9175\u7d20\u8207\u5176\u53d7\u8cea\u3001\u6297\u9ad4\u8207\u6297\u539f\u7684\u95dc\u4fc2\uff0c\u8209\u4f8b\u4f86\u8aaa\uff0c\u5229\u7528\u74ca\u8102\u7cd6(agarose)\u6216\u662f\u6709\u5b54\u6d1e\u7684\u73bb\u7483\u4f86\u593e\u5e36\u89aa\u548c\u914d\u9ad4(affinity ligand)\uff0c\u7136\u800c\u9019\u4e9b\u89aa\u548c\u914d\u9ad4\u5c0d\u6bcf\u7a2e\u5206\u5b50\u7684\u9375\u7d50\u5f37\u5ea6\u6703\u6709\u6240\u4e0d\u540c\u3002\u81f3\u65bc\u52d5\u76f8\u5247\u8981\u6709\u5169\u7a2e\u7279\u6027\uff1a\u4e00\u3001\u662f\u8981\u80fd\u8b93\u60f3\u8981\u7684\u5206\u5b50\u80fd\u8207\u89aa\u548c\u914d\u9ad4\u6709\u597d\u7684\u9375\u7d50\uff1b\u4e8c\u3001\u5247\u662f\u80fd\u5920\u6e1b\u5f31\u4e0d\u60f3\u8981\u7684\u5206\u5b50\u8207\u89aa\u548c\u914d\u9ad4\u7684\u9375\u7d50\uff0c\u901a\u5e38\u90fd\u662f\u6539\u8b8a\u9178\u9e7c\u503c\u4f86\u6539\u8b8a\u9019\u5169\u7a2e\u60c5\u5f62\u3002<\/p>\n

\u5728\u89aa\u548c\u914d\u9ad4\u7684\u9078\u64c7\u4e0a\uff0c\u6709\u4e0b\u5217\u689d\u4ef6\u70ba\u4f73\uff1a<\/p>\n

    \n
  1. \u8207\u7522\u7269\u7684\u5c08\u4e00\u6027\u3001\u89aa\u548c\u6027\u8d8a\u5927\u8d8a\u597d<\/strong><\/li>\n
  2. \u8207\u7522\u7269\u7d50\u5408\u5f8c\u5728\u4e00\u5b9a\u689d\u4ef6\u4e0b\u80fd\u89e3\u96e2\u751a\u81f3\u4e0d\u6703\u640d\u50b7\u7522\u7269\u53ca\u914d\u9ad4<\/strong><\/li>\n
  3. \u80fd\u7a69\u5b9a\u7684\u9644\u8457\u5728\u57fa\u5718\u4e0a\uff0c\u4f7f\u7522\u7269\u8207\u914d\u9ad4\u89e3\u96e2\u6642\u4e0d\u53d7\u5f71\u97ff<\/strong><\/li>\n<\/ol>\n

    \u5728\u5718\u57fa\u7684\u9078\u64c7\u4e0a\uff0c\u6709\u4e0b\u5217\u689d\u4ef6\u70ba\u4f73\uff1a<\/p>\n

      \n
    1. \u5177\u6709\u5316\u5b78\u60f0\u6027\uff0c\u4e0d\u6703\u56e0\u70ba\u9178\u9e7c\u503c\u3001\u6fc3\u5ea6\u3001\u6eab\u5ea6\u800c\u6539\u8b8a\u7d50\u69cb<\/strong><\/li>\n
    2. \u5177\u6709\u591a\u5b54\u6d1e\u4e14\u5e73\u5747\u5206\u914d<\/strong><\/li>\n<\/ol>\n
      \"64744_p1\"<\/a>

      \u5716\u4e00 \u89aa\u548c\u5c64\u6790\u793a\u610f\u5716\uff08\u4f86\u6e90\uff1a\u53c3\u8003\u8cc7\u65993\uff09<\/p><\/div>\n

      \"64744_p2\"<\/a>

      \u5716\u4e8c \u89aa\u6838\u5c64\u6790\u5716\u8b5c\u793a\u610f\u5716\uff08\u4f86\u6e90\uff1a\u53c3\u8003\u8cc7\u65993\uff09<\/p><\/div>\n

      \u7531\u5716\u4e8c\u5f97\u77e5\u7576\u6df7\u548c\u7269\u9032\u5165\u7ba1\u67f1\u5f8c\uff0c\u4e0d\u8981\u7684\u5206\u5b50\u6703\u5148\u88ab\u6c96\u63d0\u51fa\u4f86\uff0c\u63a5\u8457\u6539\u8b8a\u6c96\u63d0\u6db2\u5c31\u80fd\u5f97\u5230\u4e7e\u6de8\u4e14\u60f3\u8981\u7684\u7522\u7269\u3002\u4f46\u4e0d\u540c\u7684\u6c96\u63d0\u6db2\u6703\u8b93\u7522\u7269\u8207\u89aa\u548c\u914d\u9ad4\u7684\u65b7\u9375\u65b9\u5f0f\u4e0d\u540c\uff0c\u5982\u5716\u4e09\u6240\u793a\u3002<\/p>\n

      \"64744_p3\"<\/a>

      \u5716\u4e09 \u6c96\u63d0\u6db2\u8207\u7522\u7269\u8207\u89aa\u548c\u914d\u9ad4\u7684\u65b7\u9375\u95dc\u4fc2\uff08\u4f86\u6e90\uff1a\u53c3\u8003\u8cc7\u65993\uff09<\/p><\/div>\n

      \u800c\u8207\u4e00\u822c\u7ba1\u67f1\u5c64\u6790\u6bd4\u8f03\uff0c\u89aa\u548c\u5c64\u6790\u4e2d\u7684\u6c96\u63d0\u6db2\u6d41\u901f\u4e0d\u518d\u662f\u91cd\u9ede\uff0c\u7522\u7269\u8207\u89aa\u548c\u914d\u9ad4\u7684\u65b7\u9375\u901f\u7387\u53cd\u800c\u76f8\u5c0d\u91cd\u8981\u3002<\/p>\n

      \u89aa\u548c\u5c64\u6790\u7684\u61c9\u7528\u76f8\u7576\u5ee3\u6cdb\uff0c\u5305\u62ec\u6838\u9178\u7684\u7d14\u5316\u3001\u5f9e\u8840\u6db2\u7576\u4e2d\u7d14\u5316\u86cb\u767d\u8cea\u7b49\uff0c\u8f03\u5e38\u5229\u7528\u5728\u8840\u6e05\u6297\u9ad4\u7684\u7d14\u5316\u3002\u5982\u679c\u8840\u6e05\u7576\u4e2d\u5df2\u77e5\u542b\u6709\u6297\u7279\u5b9a\u7684\u6297\u539f\uff0c\u90a3\u9ebc\u5b83\u53ef\u5229\u7528\u8a72\u6297\u539f\u4f86\u9032\u884c\u89aa\u548c\u7d14\u5316\uff1b\u5982\u679c\u67d0\u751f\u7269\u9ad4\u88ab\u63a5\u7a2e\u67d0\u86cb\u767d\uff0c\u5176\u751f\u7269\u9ad4\u5c07\u7522\u751f\u6297\u9ad4\u91dd\u5c0d\u4e0a\u8ff0\u86cb\u767d\u4ee5\u53ca\u5c0d\u86cb\u767d\u6709\u53cd\u61c9\u7684\u6297\u9ad4\u9032\u884c\u878d\u5408\uff0c\u878d\u5408\u7684\u86cb\u767d\u8cea\u5c31\u53ef\u4ee5\u5728\u548c\u4f8b\u5982\u74ca\u8102\u7cd6\u7684\u57fa\u5718\u7d50\u5408\u5f62\u6210\u7528\u4f86\u7d14\u5316\u514d\u75ab\u8840\u6e05\u7684\u89aa\u548c\u914d\u9ad4\u3002<\/p>\n

      \n

      \u53c3\u8003\u6587\u737b<\/b><\/strong><\/p>\n

        \n
      1. \u674e\u5efa\u6b66\u7b49\u5408\u7de8\uff1b\u751f\u7269\u5316\u5b78\u5be6\u9a57\u539f\u7406\u548c\u65b9\u6cd5\uff0c\u85dd\u8ed2\u5716\u66f8\u51fa\u7248\u793e(1999). P94~110.<\/li>\n
      2. Affinity chromatography: http:\/\/en.wikipedia.org\/wiki\/Affinity_chromatography<\/li>\n
      3. GE Healthcare Life Sciences: Affinity ChromatographyPrinciples and Methods: http:\/\/www.gelifesciences.com\/file_source\/GELS\/Service%20and%20Support\/Documents%20and%20Downloads\/Handbooks\/Affinity_chromatography_handbook.pdf<\/li>\n<\/ol>\n<\/div>","protected":false},"excerpt":{"rendered":"

        \u89aa\u548c\u5c64\u6790 (Affinity chromatography) \u570b\u7acb\u81fa\u7063\u5e2b\u7bc4\u5927\u5b78\u5316\u5b78\u7cfb\u7814\u7a76\u751f \u9673\u57f9\u6770 \u4e00\u822c\u7684\u7ba1\u67f1…<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8600,6214,108,78],"tags":[9072,9074,1505,8639,9073,1504,9071],"class_list":["post-64744","post","type-post","status-publish","format-standard","hentry","category-8600","category-6214","category-chemistry00","category-chemistry08-01","tag-affinity-ligand","tag-agarose","tag-chromatography","tag-8639","tag-9073","tag-1504","tag-9071","loop-entry","cat-8600","cat-6214","cat-108","cat-78","no-thumbnail"],"views":11129,"_links":{"self":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/64744","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/comments?post=64744"}],"version-history":[{"count":1,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/64744\/revisions"}],"predecessor-version":[{"id":86428,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/64744\/revisions\/86428"}],"wp:attachment":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/media?parent=64744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/categories?post=64744"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/tags?post=64744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}