{"id":46138,"date":"2013-10-21T16:55:57","date_gmt":"2013-10-21T08:55:57","guid":{"rendered":"http:\/\/highscope.ch.ntu.edu.tw\/wordpress\/?p=46138"},"modified":"2021-10-06T16:20:00","modified_gmt":"2021-10-06T08:20:00","slug":"%e5%8f%8d%e8%bd%89%e9%8c%84%e9%85%b5%e7%b4%a0%ef%bc%88reverse-transcriptase%ef%bc%89","status":"publish","type":"post","link":"http:\/\/localhost\/%e5%8f%8d%e8%bd%89%e9%8c%84%e9%85%b5%e7%b4%a0%ef%bc%88reverse-transcriptase%ef%bc%89\/","title":{"rendered":"\u53cd\u8f49\u9304\u9175\u7d20\uff08Reverse Transcriptase\uff09"},"content":{"rendered":"

\u53cd\u8f49\u9304\u9175\u7d20\uff08Reverse Transcriptase\uff09<\/span>
\n\u570b\u7acb\u81fa\u7063\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u7814\u7a76\u52a9\u7406\u5f90\u7fe1\u66fc<\/span>
\n<\/strong><\/p>\n

\u5728\u5206\u5b50\u751f\u7269\u5b78\u4e2d\uff0c\u907a\u50b3\u4e2d\u5fc3\u6cd5\u5247\uff08central dogma\uff09\u4fc2\u6307\u53bb\u6c27\u6838\u91a3\u6838\u9178\uff08DNA\uff09\u7d93\u8f49\u9304\u4f5c\u7528\uff08transcription\uff09\u5408\u6210\u6838\u91a3\u6838\u9178\uff08RNA\uff09\uff0c\u518d\u8f49\u8b6f\uff08translation\uff09\u6210\u4f5c\u7528\u5206\u5b50\u86cb\u767d\u8cea\uff0c\u9032\u800c\u5f71\u97ff\u751f\u7269\u9ad4\u7684\u529f\u80fd\u3002\u7136\u800c\u57281970\u5e74\uff0c\u79d1\u5b78\u5bb6Howard Temin\u53caDavid Baltimore\u767c\u73fe\uff0c\u5728\u7279\u5b9a\u7684RNA\u816b\u7624\u75c5\u6bd2\u5982\u52de\u6c0f\u8089\u7624\u75c5\u6bd2\uff08R-MLV\uff09\u4e2d\uff0c\u6709\u7279\u6b8a\u7684\u53cd\u8f49\u9304\u9175\u7d20\uff08reverse transcriptase\uff09\uff0c\u80fd\u5c07\u907a\u50b3\u4fe1\u606f\u7d93\u7531\u53cd\u8f49\u9304\u4f5c\u7528\uff08reverse transcription\uff09\uff0c\u5f9eRNA\u53cd\u8f49\u9304\u6210DNA\u3002\u540c\u6a23\u7684\u53cd\u8f49\u9304\u4f5c\u7528\u4e5f\u9678\u7e8c\u88ab\u767c\u73fe\u5b58\u5728\u65bc\u771f\u6838\u751f\u7269\u4e2d\uff0c\u5982\u53cd\u8f49\u9304\u8df3\u8e8d\u5206\u5b50\uff08retrotransposon\uff09\u53ca\u7aef\u7c92\u9ad4\uff08telomere\uff09\u7684\u5408\u6210\u3002\u6b64\u767c\u73fe\u8207\u76f8\u95dc\u7814\u7a76\u57281975\u5e74\u7372\u5f97\u4e86\u8afe\u8c9d\u723e\u751f\u7269\u91ab\u5b78\u734e\u3002<\/p>\n

\u53cd\u8f49\u9304\u75c5\u6bd2<\/b><\/span><\/p>\n

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\u771f\u6838\u751f\u7269<\/b><\/span><\/p>\n

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\u5206\u5b50\u751f\u7269\u5b78\u4e2d\u7684\u61c9\u7528<\/b><\/span><\/p>\n

\u53cd\u8f49\u9304\u9175\u7d20\u7684\u767c\u73fe\u8207\u5206\u96e2\u7d14\u5316\u5c07\u805a\u5408\u9176\u93c8\u9396\u53cd\u61c9\uff08PCR\uff09\u63a8\u9032\u5230RNA\u7684\u5c64\u6b21\uff0c\u7a31\u70ba\u53cd\u8f49\u9304\u805a\u5408\u9176\u93c8\u9396\u53cd\u61c9\uff08RT-PCR\uff09\u3002\u7531\u65bcmRNA\u662f\u5177\u6709\u8f49\u9304\u610f\u7fa9\u7684\u907a\u50b3\u5206\u5b50\uff0c\u5c07\u5176\u5e8f\u5217\u53cd\u8f49\u9304\u56de\u4e92\u88dcDNA\uff0c\u518d\u4ee5\u805a\u5408\u9176\u93c8\u9396\u53cd\u61c9\u653e\u5927\uff0c\u5373\u53ef\u7372\u5f97\u4e0d\u5e36\u6709\u5167\u63d2\u5b50\uff08intron\uff09\u7684\u57fa\u56e0\u5e8f\u5217\uff0c\u4f86\u5efa\u69cb\u4e92\u88dcDNA\uff08complementary DNA\uff09\u8cc7\u6599\u5eab\uff0c\u9032\u800c\u63a2\u8a0e\u751f\u7269\u9ad4\u5167\u7684\u57fa\u56e0\u8868\u73fe\u3002\u73fe\u4eca\u4e5f\u5e38\u5229\u7528\u53cd\u8f49\u9304\u9175\u7d20\u88fd\u4f5c\u751f\u7269\u9ad4\u7684\u8f49\u9304\u9ad4\u8cc7\u6599\u5eab\u3002\u5728\u57fa\u56e0\u5de5\u7a0b\u4e0a\uff0c\u5c07\u771f\u6838\u751f\u7269\u7684\u57fa\u56e0\u5e8f\u5217\u8f49\u6b96\u5165\u7d30\u83cc\uff0c\u5373\u53ef\u5229\u7528\u7d30\u83cc\u88fd\u4f5c\u86cb\u767d\u8cea\u3002\u4f46\u7d30\u83cc\u4e0d\u5177\u6709\u88c1\u5207\u5167\u63d2\u5b50\u7684\u6a5f\u5236\uff0c\u7121\u6cd5\u88fd\u4f5c\u51fa\u7b49\u540c\u65bc\u771f\u6838\u751f\u7269\u7684\u4f5c\u7528\u86cb\u767d\u8cea\u3002\u53cd\u8f49\u9304\u9175\u7d20\u53ef\u5408\u6210\u4e92\u88dcDNA\uff0c\u5f4c\u88dc\u6b64\u9805\u7f3a\u5931\uff0c\u73fe\u4eca\u7684\u5408\u6210\u80f0\u5cf6\u7d20\u5373\u662f\u5c07\u80f0\u5cf6\u7d20\u57fa\u56e0\u8f49\u6b96\u5165\u7d30\u83cc\u88fd\u4f5c\u91cd\u7d44\u86cb\u767d\uff0c\u5927\u5e45\u964d\u4f4e\u80f0\u5cf6\u7d20\u7684\u50f9\u683c\uff0c\u662f\u7cd6\u5c3f\u75c5\u4eba\u7684\u4e00\u5927\u798f\u97f3\u3002<\/p>\n

 <\/p>\n

——————–<\/p>\n

\u53c3\u8003\u8cc7\u6599<\/b><\/span><\/p>\n

    \n
  1. http:\/\/en.wikipedia.org\/wiki\/Reverse_transcriptase<\/a><\/span><\/li>\n
  2. Nicholas H. Acheson, Foundamentals of Molecular Virology, Wiley.<\/span><\/li>\n
  3. Patrick R. Murray, Ken S. Rosenthal, George S. Kobayashi, Michael A. Pfaller, Medical Microbiology, Elsevier.<\/span><\/li>\n<\/ol>\n

     <\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

    \u53cd\u8f49\u9304\u9175\u7d20\uff08Reverse Transcriptase\uff09 \u570b\u7acb\u81fa\u7063\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u7814\u7a76\u52a9\u7406\u5f90\u7fe1\u66fc \u5728\u5206\u5b50\u751f\u7269\u5b78\u4e2d…<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4810,110,4818],"tags":[5512,5511,5513],"class_list":["post-46138","post","type-post","status-publish","format-standard","hentry","category-lifesciecne001","category-lifesciecne00","category-lifesciecne001-02","tag-reverse-transcriptase","tag-5511","tag-5513","loop-entry","cat-4810","cat-110","cat-4818","no-thumbnail"],"views":33215,"_links":{"self":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/46138","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/comments?post=46138"}],"version-history":[{"count":1,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/46138\/revisions"}],"predecessor-version":[{"id":87382,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/46138\/revisions\/87382"}],"wp:attachment":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/media?parent=46138"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/categories?post=46138"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/tags?post=46138"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}