{"id":42990,"date":"2013-08-27T17:20:13","date_gmt":"2013-08-27T09:20:13","guid":{"rendered":"http:\/\/highscope.ch.ntu.edu.tw\/wordpress\/?p=42990"},"modified":"2021-10-06T16:20:44","modified_gmt":"2021-10-06T08:20:44","slug":"%e8%bd%89%e5%bd%a2%e4%bd%9c%e7%94%a8%ef%bc%88transformation%ef%bc%89","status":"publish","type":"post","link":"http:\/\/localhost\/%e8%bd%89%e5%bd%a2%e4%bd%9c%e7%94%a8%ef%bc%88transformation%ef%bc%89\/","title":{"rendered":"\u8f49\u5f62\u4f5c\u7528"},"content":{"rendered":"

\u8f49\u5f62\u4f5c\u7528\uff08Transformation\uff09<\/span>
\n<\/strong>\u570b\u7acb\u81fa\u7063\u5e2b\u7bc4\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u7814\u7a76\u52a9\u7406\u6797\u5982\u6114<\/span><\/strong><\/p>\n

\u8f49\u5f62\u4f5c\u7528\uff08\u4e5f\u7a31\u8f49\u5316\u4f5c\u7528\uff09\u662f\u5f9e\u7d30\u80de\u819c\u76f4\u63a5\u5c07\u7570\u6e90DNA\u5206\u5b50\u7d0d\u5165\u7d30\u80de\u5167\uff0c\u4f7f\u53d7\u9ad4\u7d30\u80de\u7372\u5f97\u65b0\u7684\u907a\u50b3\u6027\u72c0\u7684\u65b9\u6cd5\u4e4b\u4e00\uff0c\u4ee5\u7d30\u83cc\u800c\u8a00\uff0c\u53e6\u5916\u5169\u500b\u5e38\u898b\u7684\u65b9\u6cd5\u5206\u5225\u662f\u63a5\u5408\u4f5c\u7528\uff08conjugation\uff09\u8207\u8f49\u5c0e\u4f5c\u7528\uff08transduction\uff09\uff0c\u70ba\u5fae\u751f\u7269\u907a\u50b3\u5b78\u3001\u5206\u5b50\u907a\u50b3\u5b78\u3001\u57fa\u56e0\u5de5\u7a0b\u7b49\u7814\u7a76\u9818\u57df\u7684\u57fa\u672c\u5be6\u9a57\u6280\u8853\u3002<\/p>\n

1928\u5e74\uff0c\u82f1\u570b\u7684\u683c\u91cc\u592b\u8332\uff08Frederick Griffith\uff09\u9996\u5148\u767c\u73fe\u8f49\u5f62\u4f5c\u7528\u3002\u683c\u91cc\u592b\u8332\u5229\u7528\u80ba\u708e\u93c8\u7403\u83cc\uff08Streptococcus pneumoniae<\/em>\uff09\u7684\u5169\u500b\u4e0d\u540c\u83cc\u682a\u70ba\u6750\u6599\uff0c\u4e00\u70ba\u5177\u6709\u83a2\u819c\u4e14\u53ef\u81f4\u75c5\u7684S\u83cc\u682a\uff08smooth strain, S strain\uff09\uff0c\u4e00\u500b\u662f\u4e0d\u5177\u83a2\u819c\u4e14\u4e0d\u6703\u81f4\u75c5\u7684R\u83cc\u682a\uff08rough strain, R strain\uff09\u3002\u5982\u5716\u4e00\u6240\u793a\uff0cR\u83cc\u682a\u6253\u5165\u5c0f\u9f20\u4e2d\u4e0d\u6703\u81f4\u75c5\uff0c\u800c\u6ce8\u5c04S\u83cc\u682a\u7684\u8001\u9f20\u6703\u767c\u75c5\u6b7b\u4ea1\u3002\u683c\u91cc\u592b\u8332\u5c07S\u83cc\u682a\u7d93\u904e\u52a0\u71b1\u6bba\u83cc\u5f8c\uff0c\u6ce8\u5c04\u5165\u6d3b\u9f20\u9ad4\u5167\uff0c\u4e26\u4e0d\u6703\u4f7f\u8001\u9f20\u767c\u75c5\uff1b\u4f46\u662f\u5c07\u52a0\u71b1\u904e\u7684S\u83cc\u682a\u8207\u6d3b\u7684R\u83cc\u682a\u6df7\u5408\u518d\u6ce8\u5165\u5c0f\u9f20\u9ad4\u5167\uff0c\u5c0f\u9f20\u537b\u6703\u767c\u75c5\u6b7b\u4ea1\uff0c\u683c\u91cc\u592b\u8332\u5728\u6b64\u7d44\u6b7b\u8001\u9f20\u7684\u8840\u6db2\u4e2d\u767c\u73fe\u6709\u5177\u83a2\u819c\u7684\u6d3b\u83cc\uff08S strain\uff09\u5b58\u5728\uff0c\u4ed6\u61f7\u7591\u6ce8\u5c04\u524d\u7684R\u83cc\u682a\u56e0\u70ba\u7279\u6b8a\u539f\u56e0\u800c\u7372\u5f97\u4e86S\u83cc\u682a\u7684\u7279\u6027\uff0c\u8f49\u5316\u6210\u5177\u6709\u83a2\u819c\u7684\u81f4\u75c5\u83cc\u682a\u3002<\/p>\n

\"\"<\/a>

\u5716\u4e00 \u683c\u91cc\u592b\u8332\u7684\u57fa\u56e0\u8f49\u5f62\u5be6\u9a57<\/p><\/div>\n

\u70ba\u8b49\u5be6\u81ea\u5df1\u7684\u63a8\u8ad6\uff0c\u683c\u91cc\u592b\u8332\u53c8\u505a\u4e86\u53e6\u4e00\u500b\u5be6\u9a57\uff0c\u4ed6\u4e0d\u518d\u4f7f\u7528\u6d3b\u9f20\uff0c\u800c\u662f\u5c07R\u83cc\u682a\u63a5\u7a2e\u65bc\u57f9\u990a\u6db2\u4e2d\uff0c\u7136\u5f8c\u518d\u52a0\u5165\u5177\u83a2\u819c\uff08S\u83cc\u682a\uff09\u7684\u6b7b\u83cc\uff0c\u7d93\u904e\u57f9\u990a\u767c\u73fe\uff0c\u57f9\u990a\u57fa\u4e2d\u53ef\u4ee5\u5206\u96e2\u51fa\u5177\u83a2\u819c\u7684\u81f4\u75c5\u83cc-S\u83cc\u682a\u3002\u9019\u7d50\u679c\u986f\u793a\uff0cR\u83cc\u682a\u5df2\u88ab\u8f49\u5f62\uff0c\u5176\u7d93\u7531\u57f9\u990a\u6db2\u4e2d\u7684S\u83cc\u682a\u6b7b\u83cc\u7372\u5f97\u81f4\u75c5\u57fa\u56e0\uff0c\u539f\u672c\u4e0d\u5177\u81f4\u75c5\u6027\u7684R\u83cc\u682a\u4e4b\u907a\u50b3\u7279\u6027\u6539\u8b8a\uff0c\u8f49\u6210\u80fd\u7522\u751f\u83a2\u819c\u4e14\u6709\u81f4\u75c5\u529b\u7684S\u83cc\u682a\u3002\u4f46\u7576\u6642\u683c\u91cc\u592b\u8332\u4e26\u4e0d\u77e5\u9053\u8f49\u5f62\u4f5c\u7528\u662f\u7531\u6b7b\u83cc\u4e2d\u7684\u4f55\u7a2e\u7269\u8cea\u6240\u9020\u6210\u3002\u76f4\u52301944\u5e74\uff0c\u624d\u7531\u7f8e\u570b\u5b78\u8005\u57c3\u5f17\u91cc\uff08Oswald T. Avery\uff09\u7684\u7814\u7a76\u8b49\u5be6\uff0c\u5c0e\u81f4\u80ba\u708e\u93c8\u7403\u83ccR\u83cc\u682a\u8f49\u5f62\u6210\u81f4\u75c5S\u83cc\u682a\u7684\u7269\u8cea\u70baDNA\u3002<\/p>\n

\u5728\u81ea\u7136\u60c5\u6cc1\u4e0b\uff0c\u6975\u5c11\u7a2e\u985e\u7684\u7d30\u83cc\u6703\u767c\u751f\u8f49\u5f62\u4f5c\u7528\u3002\u73fe\u5728\u4ee5\u4eba\u5de5\u7684\u65b9\u6cd5\u4e5f\u80fd\u9054\u6210\u8f49\u5f62\u4f5c\u7528\uff0c\u9996\u5148\u662f\u5c07\u7d30\u83cc\u88fd\u4f5c\u6210\u52dd\u4efb\u7d30\u80de\uff08competent cell\uff09\uff0c\u7d93\u904e\u4e00\u4e9b\u7279\u6b8a\u65b9\u6cd5\uff0c\u50cf\u662f\u96fb\u64ca\u3001CaCl2<\/sub>\u3001RuCl\u7b49\u5316\u5b78\u8a66\u5291\u7684\u8655\u7406\u5f8c\uff0c\u7d30\u80de\u819c\u7684\u901a\u900f\u6027\u767c\u751f\u8b8a\u5316\uff0c\u6210\u70ba\u80fd\u5bb9\u8a31\u5916\u6e90DNA\u901a\u904e\u7684\u52dd\u4efb\u7d30\u80de\u3002\u5728\u4e00\u5b9a\u689d\u4ef6\u4e0b\uff0c\u5c07\u5916\u6e90DNA\u8207\u52dd\u4efb\u7d30\u80de\u6df7\u5408\u4fdd\u6eab\uff0c\u4f7f\u5916\u6e90DNA \u5206\u5b50\u9032\u5165\u53d7\u9ad4\u7d30\u80de\u3002\u9032\u5165\u7d30\u80de\u7684DNA\u5206\u5b50\u7d93\u904e\u8907\u88fd\u3001\u8f49\u9304\u3001\u8f49\u8b6f\u7b49\u751f\u5316\u904e\u7a0b\uff0c\u4f7f\u53d7\u9ad4\u7d30\u80de\u51fa\u73fe\u65b0\u7684\u907a\u50b3\u6027\u72c0\u3002\u7d93\u904e\u8f49\u5f62\u5f8c\u7684\u7d30\u80de\uff0c\u4ea6\u5373\u5e36\u6709\u7570\u6e90DNA\u7684\u53d7\u9ad4\u7d30\u80de\uff0c\u4ee5\u91cd\u7d44\u4f5c\u7528\u5c07\u4e4b\u4f75\u5165\u5176\u672c\u8eab\u7684\u67d3\u8272\u9ad4\u4e2d\uff0c\u53c8\u7a31\u70ba\u91cd\u7d44\u7d30\u80de\uff08recombinant cell\uff09\u3002<\/p>\n

\u8f49\u5f62\u4f5c\u7528\u662f\u5206\u5b50\u751f\u7269\u5b78\u9818\u57df\u6975\u70ba\u91cd\u8981\u7684\u57fa\u790e\u6280\u8853\u3002\u85c9\u7531\u5c07\u7279\u5b9a\u57fa\u56e0\u9001\u5165\u7d30\u80de\uff0c\u6211\u5011\u5f97\u4ee5\u5c0d\u7279\u5b9a\u57fa\u56e0\u7684\u529f\u80fd\u9032\u884c\u89c0\u5bdf\u7814\u7a76\uff0c\u4e5f\u5f97\u4ee5\u5927\u91cf\u8868\u73fe\u6709\u7d93\u6fdf\u50f9\u503c\u4e4b\u57fa\u56e0\u7522\u7269\uff0c\u50cf\u662f\u91ab\u7528\u80f0\u5cf6\u7d20\u7684\u751f\u7522\u3002\u8f49\u5f62\u6280\u8853\u53ef\u4ee5\u8aaa\u662f\u958b\u555f\u4e86\u5206\u5b50\u751f\u7269\u5b78\u8207\u5206\u5b50\u91ab\u5b78\u7814\u7a76\u4e4b\u5927\u9580\u3002<\/p>\n

\n

\u53c3\u8003\u8cc7\u6599<\/strong><\/p>\n

    \n
  1. Alberts, Bruce et al. (2002). Molecular Biology of the Cell. New York: Garland Science. p. G:35.<\/li>\n
  2. Frederick Griffith http:\/\/applejacksgirl.blogspot.tw\/2012\/04\/frederick-griffith.html<\/a><\/li>\n
  3. Lederberg, Joshua (1994). The Transformation of Genetics by DNA: An Anniversary Celebration of AVERY, MACLEOD and MCCARTY (1944) in Anecdotal, Historical and Critical Commentaries on Genetics. The Rockfeller University, New York, New York 10021-6399.<\/li>\n
  4. Transformation (genetics) \u00a0http:\/\/en.wikipedia.org\/wiki\/Transformation_(genetics)<\/a><\/li>\n<\/ol>\n<\/div>","protected":false},"excerpt":{"rendered":"

    \u8f49\u5f62\u4f5c\u7528\uff08Transformation\uff09 \u570b\u7acb\u81fa\u7063\u5e2b\u7bc4\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u7814\u7a76\u52a9\u7406\u6797\u5982\u6114 \u8f49\u5f62\u4f5c\u7528\uff08\u4e5f\u7a31\u8f49\u5316\u4f5c\u7528\uff09\u662f…<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4814,110,3967],"tags":[8497,4781,4780,4779,4778,4777],"class_list":["post-42990","post","type-post","status-publish","format-standard","hentry","category-lifesciecne005","category-lifesciecne00","category-lifesciecne005-03","tag-transformation","tag-4781","tag-4780","tag-4779","tag-4778","tag-4777","loop-entry","cat-4814","cat-110","cat-3967","no-thumbnail"],"views":46700,"_links":{"self":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/42990","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/comments?post=42990"}],"version-history":[{"count":1,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/42990\/revisions"}],"predecessor-version":[{"id":87560,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/42990\/revisions\/87560"}],"wp:attachment":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/media?parent=42990"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/categories?post=42990"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/tags?post=42990"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}