{"id":42211,"date":"2013-04-10T17:11:17","date_gmt":"2013-04-10T09:11:17","guid":{"rendered":"http:\/\/highscope.ch.ntu.edu.tw\/wordpress\/?p=42211"},"modified":"2021-10-06T16:21:48","modified_gmt":"2021-10-06T08:21:48","slug":"%e5%ae%9a%e5%90%91%e6%bc%94%e5%8c%96%ef%bc%88directed-evolution%ef%bc%89","status":"publish","type":"post","link":"http:\/\/localhost\/%e5%ae%9a%e5%90%91%e6%bc%94%e5%8c%96%ef%bc%88directed-evolution%ef%bc%89\/","title":{"rendered":"\u5b9a\u5411\u6f14\u5316\uff08Directed evolution\uff09"},"content":{"rendered":"<div class=\"pf-content\"><p><strong><span style=\"color: #ff6600;\">\u5b9a\u5411\u6f14\u5316\uff08Directed evolution\uff09<\/span><br \/>\n<\/strong><strong><span style=\"color: #008000;\">\u570b\u7acb\u81fa\u7063\u5e2b\u7bc4\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u7814\u7a76\u52a9\u7406\u912d\u674f\u5029<\/span><\/strong><\/p>\n<p>\u5b9a\u5411\u6f14\u5316\uff08directed evolution\uff09\u662f\u6539\u9020\u86cb\u767d\u8cea\u5206\u5b50\u7684\u4e00\u7a2e\u6709\u6548\u7b56\u7565\uff0c\u5229\u7528\u5728\u6d3b\u9ad4\u5916\uff08in vitro\uff09\u9032\u884c\u5be6\u9a57\uff0c\u5c07\u5e36\u6709\u76ee\u6a19\u86cb\u767d\u8cea\u7684\u57fa\u56e0\u5e8f\u5217\u9032\u884c\u985e\u4f3c\u5929\u64c7\u7684\u9078\u6c70\u4f5c\u7528\uff08natural selection\uff09\uff0c\u4f7f\u4e4b\u7372\u5f97\u5728\u81ea\u7136\u5929\u64c7\u72c0\u6cc1\u4e0b\u7121\u6cd5\u5f97\u5230\u3001\u4e14\u5177\u6709\u7279\u6b8a\u529f\u80fd\u7684\u57fa\u56e0\u7522\u7269\u3002\u6b64\u7a2e\u64cd\u4f5c\u6280\u8853\u4e3b\u8981\u61c9\u7528\u65bc\u86cb\u767d\u8cea\u5de5\u7a0b\uff08protein engineering\uff09\u7684\u5be6\u9a57\u4e2d\u3002<\/p>\n<p>\u5be6\u9a57\u64cd\u4f5c\u4e0a\uff0c\u5148\u5efa\u7acb\u8d77\u4e00\u500b\u7d93\u904e\u96a8\u6a5f\uff08randomized\uff09\u7a81\u8b8a\u6216\u662f\u91cd\u65b0\u7d44\u5408\u7684\u57fa\u56e0\u5eab\uff08library\uff09\uff0c\u63a5\u8457\u7be9\u9078\uff08screen\/selection\uff09\u51fa\u53ef\u4ee5\u8f49\u9304\/\u8f49\u8b6f\u51fa\u6240\u9700\u8981\u529f\u80fd\u7684\u86cb\u767d\u8cea\u4e4b\u57fa\u56e0\u5e8f\u5217\u3002\u8a73\u7d30\u7684\u64cd\u4f5c\u65b9\u5f0f\u4e3b\u8981\u6709\u4ee5\u4e0b\u4e09\u500b\u6b65\u9a5f\uff1a<\/p>\n<ol>\n<li><strong>\u591a\u6a23\u5316\uff08diversification\uff09<\/strong>\uff1a\u5229\u7528error-prone PCR\u6216\u662fDNA shuffling\u2026\u7b49\u65b9\u5f0f\u5c07\u76ee\u6a19\u86cb\u767d\u8cea\u7684\u57fa\u56e0\u5e8f\u5217\u9032\u884c\u96a8\u6a5f\u7684\u7a81\u8b8a\uff08\u9ede\u7a81\u8b8apoint mutation\u3001\u63d2\u5165insertion\u6216\u662f\u522a\u9664deletion\uff09\u6216\u662f\u91cd\u7d44\uff08recombination\uff09\uff0c\u5f97\u5230\u4e00\u500b\u5177\u6709\u5927\u91cf\u4e14\u591a\u6a23\u6027\u9ad8\u7684\u57fa\u56e0\u5eab\u3002<\/li>\n<li><strong>\u9078\u6c70\uff08selection\uff09<\/strong>\uff1a\u63a5\u8457\uff0c\u5c07\u4e0a\u8ff0\u7684\u57fa\u56e0\u5eab\u4e2d\u7684\u7a81\u8b8a\u9ad4\uff08mutant\/variant\uff09\u9032\u884c\u86cb\u767d\u8cea\u8868\u9054\u3002\u7be9\u9078\u7684\u65b9\u5f0f\u5f88\u591a\u6a23\u5316\uff0c\u76ee\u7684\u662f\u8981\u7be9\u9078\u51fa\u5177\u6709\u7279\u5b9a\u529f\u80fd\u4e4b\u57fa\u56e0\u5e8f\u5217\uff0c\u4e26\u540c\u6642\u522a\u9664\u5176\u9918\u7684\u5e8f\u5217\u3002<\/li>\n<li><strong>\u653e\u5927\uff08amplification\uff09<\/strong>\uff1a\u5c07\u76ee\u6a19\u7a81\u8b8a\u9ad4\u57fa\u56e0\u653e\u5927\u4e26\u9032\u884c\u8907\u88fd\uff0c\u4e26\u5c0d\u8a72\u7a81\u8b8a\u9ad4\u9032\u884c\u7814\u7a76\u3002<\/li>\n<\/ol>\n<p>\u4ee5\u4e0a\u4e09\u500b\u6b65\u9a5f\u70ba\u4e00\u500b\u55ae\u4f4d\uff0c\u4e0a\u4e00\u8f2a\u9078\u51fa\u4f86\u7684\u7a81\u8b8a\u9ad4\u88ab\u62ff\u4f86\u9032\u884c\u4e0b\u4e00\u8f2a\u5be6\u9a57\u7684DNA\u6a21\u677f\uff08template\uff09\uff0c\u7d93\u7531\u91cd\u8907\u5e7e\u8f2a\u7684\u5be6\u9a57\u5f8c\uff0c\u5f97\u5230\u5177\u6709\u826f\u597d\u529f\u80fd\u57fa\u56e0\u3002\u6b64\u6280\u8853\u5e38\u61c9\u7528\u5728protein-ligand binding\u3001\u63d0\u9ad8\u86cb\u767d\u8cea\u7a69\u5b9a\u6027\u4ee5\u53ca\u4fee\u6539\u9175\u7d20\u7d50\u69cb\u7b49\u7814\u7a76\u9818\u57df\uff0c\u4e26\u4e14\u61c9\u7528\u5728\u764c\u75c7\u85e5\u7269\u958b\u767c\u3001\u751f\u8cea\u71c3\u6599\uff08biofuel\uff09\u8f49\u63db\u7b49\u8b70\u984c\u4e0a\u3002<!--more--><\/p>\n<p>\u5b9a\u5411\u6f14\u5316\u4e0d\u50c5\u5728\u5de5\u696d\u3001\u91ab\u5b78\u65b9\u9762\u5c0d\u86cb\u767d\u8cea\u7684\u6539\u8b8a\u5177\u6709\u91cd\u8981\u7684\u610f\u7fa9\uff0c\u540c\u6642\u4e5f\u975e\u5e38\u9069\u5408\u65bc\u57fa\u790e\u7406\u8ad6\u7684\u7814\u7a76\u3002\u5206\u6790\u7a81\u8b8a\u9ad4\uff0c\u5c07\u53ef\u4ee5\u5c0d\u86cb\u767d\u8cea\u7684\u7d50\u69cb\u548c\u529f\u80fd\u9593\u7684\u95dc\u4fc2\u63d0\u4f9b\u65b0\u7684\u5de5\u5177\u3002\u5728\u81ea\u7136\u7684\u72c0\u6cc1\u4e0b\uff0c\u86cb\u767d\u8cea\u5e8f\u5217\u7684\u591a\u578b\u6027\uff08polymorphism\uff09\u901a\u5e38\u662f\u4f86\u81ea\u96a8\u6a5f\u7684\u907a\u50b3\u6f02\u8b8a\uff08genetic drift\uff09\u800c\u975e\u9078\u6c70\u7684\u7d50\u679c\uff08\u4ea6\u5373\u4e26\u975e\u5c6c\u65bc\u529f\u80fd\u6027\u7684\u9069\u61c9\uff09\u3002\u5f9e\u5df2\u7d93\u9069\u61c9\u4e0d\u540c\u74b0\u5883\u689d\u4ef6\u7684\u7269\u7a2e\u9032\u884c\u86cb\u767d\u8cea\u5e8f\u5217\u5206\u6790\uff0c\u767c\u73fe\u5927\u90e8\u5206\u7684\u8b8a\u7570\u90fd\u8207\u529f\u80fd\u8868\u73fe\u6c92\u6709\u592a\u5927\u7684\u95dc\u4fc2\uff0c\u50c5\u6709\u4e00\u5c0f\u90e8\u5206\u662f\u8207\u7279\u5b9a\u529f\u80fd\u5dee\u7570\u6709\u95dc\u3002\u800c\u5927\u91cf\u7684\u4e2d\u6027\u7a81\u8b8a\u6703\u5e72\u64fe\u7814\u7a76\u4eba\u54e1\u8fa8\u5225\u86cb\u767d\u8cea\u5206\u5b50\u7684\u898f\u5f8b\u3002\u7136\u800c\uff0c\u5728\u5be6\u9a57\u4e2d\u6240\u4f7f\u7528\u7684\u5b9a\u5411\u6f14\u5316\u7b56\u7565\uff0c\u5176\u5229\u7528\u76ee\u7684\u53d6\u5411\u7684\u6311\u9078\u65b9\u5f0f\uff0c\u53ef\u4ee5\u5f88\u6e05\u695a\u7684\u5f97\u77e5\u5176\u8b8a\u7570\u6240\u9020\u6210\u7684\u86cb\u767d\u8cea\u529f\u80fd\u6539\u8b8a\u3002Arnold\u7814\u7a76\u5718\u968a\u5229\u7528\u6b64\u7814\u7a76\u65b9\u5f0f\u6210\u529f\u7684\u5340\u5206\u57fa\u56e0\u4e2d\u7684\u529f\u80fd\u6027\u548c\u975e\u529f\u80fd\u6027\u7a81\u8b8a\uff0c\u4e26\u900f\u904e\u5b9a\u5411\u6f14\u5316\u7814\u7a76\u86cb\u767d\u8cea\u7684\u7d50\u69cb\u548c\u529f\u80fd\u7684\u95dc\u4fc2\uff0c\u6b64\u53ef\u4ee5\u70ba\u86cb\u767d\u8cea\u7684\u8a2d\u8a08\u63d0\u4f9b\u7406\u8ad6\u4f9d\u64da\u3002<\/p>\n<p>\u53c3\u8003\u8cc7\u6599\uff1a<br \/>\n\u90ed\u9cf3\u5a1f\u30022004\u3002\u86cb\u767d\u8cea\u7684\u5b9a\u5411\u9032\u5316\u53ca\u61c9\u7528\u3002\u4e2d\u570b\u751f\u5316\u85e5\u7269\u96dc\u8a8c25\uff082\uff09\uff1a125-126\u3002Reviews: Directed evolution\/Library construction. <a href=\"http:\/\/openwetware.org\/wiki\/Reviews:Directed_evolution\/Library_construction\">http:\/\/openwetware.org\/wiki\/Reviews:Directed_evolution\/Library_construction<\/a><\/p>\n<p>Directed evolution. http:\/\/en.wikipedia.org\/wiki\/Directed_evolution<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>\u5b9a\u5411\u6f14\u5316\uff08Directed evolution\uff09 \u570b\u7acb\u81fa\u7063\u5e2b\u7bc4\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u7814\u7a76\u52a9\u7406\u912d\u674f\u5029 \u5b9a\u5411\u6f14\u5316\uff08dire&hellip;<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[110,4815,4842],"tags":[4650,3106],"class_list":["post-42211","post","type-post","status-publish","format-standard","hentry","category-lifesciecne00","category-lifesciecne006","category-lifesciecne006-04","tag-4650","tag-3106","loop-entry","cat-110","cat-4815","cat-4842","no-thumbnail"],"views":6420,"_links":{"self":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/42211","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/comments?post=42211"}],"version-history":[{"count":1,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/42211\/revisions"}],"predecessor-version":[{"id":87656,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/posts\/42211\/revisions\/87656"}],"wp:attachment":[{"href":"http:\/\/localhost\/wp-json\/wp\/v2\/media?parent=42211"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/categories?post=42211"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/localhost\/wp-json\/wp\/v2\/tags?post=42211"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}