{"id":35753,"date":"2020-10-09T00:16:48","date_gmt":"2020-10-08T16:16:48","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=35753"},"modified":"2020-12-02T10:09:06","modified_gmt":"2020-12-02T02:09:06","slug":"%e3%80%902020%e8%ab%be%e8%b2%9d%e7%88%be%e5%8c%96%e5%ad%b8%e7%8d%8e%e3%80%91%e5%9f%ba%e5%9b%a0%e5%89%aa%e5%88%80%ef%bc%9a%e4%b8%80%e5%80%8b%e6%94%b9%e5%af%ab%e7%94%9f%e5%91%bd%e5%af%86%e7%a2%bc","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=35753","title":{"rendered":"\u30102020\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u3011\u57fa\u56e0\u526a\u5200\uff1a\u4e00\u500b\u6539\u5beb\u751f\u547d\u5bc6\u78bc\u7684\u5de5\u5177"},"content":{"rendered":"

\u827e\u66fc\u7d10\u723e\u2022\u590f\u672c\u63d0\u723e\uff08Emmanuelle Charpentier\uff09\u8207\u73cd\u59ae\u4f5b\u2022\u9053\u7d0d\uff08Jennifer Doudna\uff09\u69ae\u7372\u4e862020\u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u7684\u6842\u51a0\uff0c\u4e3b\u8981\u662f\u56e0\u70ba\u5979\u5011\u767c\u73fe\u4e86\u57fa\u56e0\u6280\u8853\u4e2d\u6700\u5f37\u5927\u7684\u5de5\u5177\u4e4b\u4e00\uff1aCRISPR\/Cas9\u57fa\u56e0\u526a\u5200\u3002\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u975e\u5e38\u7cbe\u6e96\u5730\u4f7f\u7528\u5b83\u5011\u6539\u8b8a\u52d5\u7269\u3001\u690d\u7269\u548c\u5fae\u751f\u7269\u7684DNA(\u53bb\u6c27\u6838\u7cd6\u6838\u9178)\u3002\u9019\u500b\u6280\u8853\u5fb9\u5e95\u6539\u8b8a\u4e86\u5206\u5b50\u751f\u547d\u79d1\u5b78\uff0c\u70ba\u690d\u7269\u80b2\u7a2e\u5e36\u4f86\u4e86\u65b0\u6a5f\u6703\u3001\u6709\u52a9\u65bc\u5275\u65b0\u7684\u764c\u75c7\u7642\u6cd5\u3001\u4e26\u53ef\u80fd\u4f7f\u6cbb\u7652\u907a\u50b3\u6027\u75be\u75c5\u7684\u5922\u60f3\u6210\u771f\u3002<\/strong><\/span><\/p>\n

\"\"
\u57161  \u4f7f\u7528\u57fa\u56e0\u526a\u5200\uff0c\u7814\u7a76\u4eba\u54e1\u80fd\u7de8\u8f2f\u6240\u6709\u751f\u7269\u7684\u57fa\u56e0\u9ad4\u3002<\/figcaption><\/figure>\n

\u7de8\u8b6f\uff0f\u8521\u860a\u660e<\/strong><\/p>\n

\u79d1\u5b78\u7684\u5438\u5f15\u529b\u4e4b\u4e00\u662f\u5b83\u96e3\u4ee5\u9810\u6e2c\u2013\u4f60\u6c38\u9060\u7121\u6cd5\u9810\u77e5\u4e00\u500b\u60f3\u6cd5\u6216\u554f\u984c\u6703\u5c07\u4f60\u5f15\u81f3\u4f55\u8655\u3002\u6709\u6642\u5019\u597d\u5947\u7684\u5fc3\u6703\u9047\u5230\u6b7b\u80e1\u540c\uff0c\u6709\u6642\u5247\u6703\u9047\u5230\u68d8\u624b\u7684\u8ff7\u5bae\uff0c\u9700\u8981\u82b1\u8cbb\u591a\u5e74\u7684\u6642\u9593\u5728\u5176\u4e2d\u63a2\u7d22\u3002\u4f46\u662f\u4e00\u6b21\u53c8\u4e00\u6b21\u5730\uff0c\u5979\u610f\u8b58\u5230\u81ea\u5df1\u662f\u7b2c\u4e00\u500b\u51dd\u8996\u8457\u4e00\u500b\u672a\u77e5\u7684\u53ef\u80fd\u6027\u5f9e\u5730\u5e73\u7dda\u4e0a\u5347\u8d77\u7684\u4eba\u3002<\/p>\n

\u9019\u500b\u88ab\u7a31\u70baCRISPR-Cas9\u800c\u5177\u6709\u9a5a\u4eba\u6f5b\u529b\u7684\u57fa\u56e0\u7de8\u8f2f\u5668\uff0c\u5c31\u662f\u9019\u6a23\u7684\u4e00\u500b\u610f\u5916\u767c\u73fe\u3002\u7576\u590f\u672c\u63d0\u723e\u548c\u9053\u7d0d\u958b\u59cb\u7814\u7a76\u4e00\u500b\u93c8\u7403\u83cc\u5c6c\u7d30\u83cc\u7684\u514d\u75ab\u7cfb\u7d71\u6642\uff0c\u4e00\u500b\u60f3\u6cd5\u662f\u5979\u5011\u53ef\u80fd\u958b\u767c\u51fa\u4e00\u7a2e\u65b0\u7684\u6297\u751f\u7d20\u3002\u76f8\u53cd\u7684\uff0c\u5979\u5011\u537b\u767c\u73fe\u4e86\u4e00\u500b\u5206\u5b50\u5de5\u5177\uff0c\u53ef\u7528\u65bc\u7cbe\u6e96\u7684\u5207\u5272\u57fa\u56e0\u7269\u8cea\uff0c\u4f7f\u5979\u5011\u53ef\u4ee5\u8f15\u6613\u5730\u6539\u8b8a\u751f\u547d\u7684\u5bc6\u78bc\u3002<\/p>\n

\u25cf\u4e00\u500b\u5f71\u97ff\u6240\u6709\u4eba\u7684\u5f37\u5927\u5de5\u5177<\/strong><\/span><\/p>\n

\u5728\u9019\u500b\u767c\u73fe\u5f8c\u50c5\u50c5\u516b\u5e74\uff0c\u9019\u4e9b\u57fa\u56e0\u526a\u5200\u5c31\u91cd\u5851\u4e86\u751f\u547d\u79d1\u5b78\u3002\u751f\u7269\u5316\u5b78\u5bb6\u8207\u7d30\u80de\u751f\u7269\u5b78\u5bb6\u73fe\u5728\u53ef\u4ee5\u8f15\u6613\u5730\u7814\u7a76\u4e0d\u540c\u57fa\u56e0\u7684\u529f\u80fd\uff0c\u4ee5\u53ca\u5b83\u5011\u5728\u75be\u75c5\u9032\u5c55\u4e2d\u7684\u53ef\u80fd\u89d2\u8272\u3002\u5728\u690d\u7269\u80b2\u7a2e\u4e2d\uff0c\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u8ce6\u4e88\u690d\u7269\u7279\u5b9a\u7684\u6027\u8cea\uff0c\u4f8b\u5982\u5728\u6eab\u6696\u7684\u6c23\u5019\u4e0b\u5177\u6709\u6297\u65f1\u80fd\u529b\u3002\u5728\u91ab\u5b78\u4e0a\uff0c\u9019\u500b\u57fa\u56e0\u7de8\u8f2f\u5668\u6b63\u5728\u70ba\u764c\u75c7\u63d0\u4f9b\u65b0\u7684\u7642\u6cd5\uff0c\u4ee5\u53ca\u904b\u7528\u5728\u4f01\u5716\u6cbb\u7652\u907a\u50b3\u6027\u75be\u75c5\u7684\u9996\u6279\u7814\u7a76\u4e2d\u3002<\/p>\n

\u5be6\u5728\u6709\u592a\u591a\u7684\u4f8b\u5b50\u662f\u95dc\u65bcCRISPR-Cas9\u5982\u4f55\u7684\u4f7f\u7528\uff0c\u5176\u4e2d\u9084\u5305\u62ec\u4e0d\u9053\u5fb7\u7684\u61c9\u7528\u65b9\u6cd5\u3002\u8207\u6240\u6709\u5f37\u5927\u7684\u6280\u8853\u4e00\u6a23\uff0c\u9019\u4e9b\u57fa\u56e0\u526a\u5200\u9700\u8981\u88ab\u7ba1\u63a7\uff0c\u6709\u95dc\u9019\u90e8\u5206\uff0c\u5f8c\u6587\u6703\u6709\u66f4\u591a\u8aaa\u660e\u3002<\/p>\n

\u57282011\u5e74\u6ce2\u591a\u9ece\u5404\u7684\u4e00\u5bb6\u5496\u5561\u9928\u88e1\uff0c\u590f\u672c\u63d0\u723e\u548c\u9053\u7d0d\u90fd\u4e0d\u77e5\u9053\u5979\u5011\u7684\u7b2c\u4e00\u6b21\u6703\u9762\uff0c\u5c07\u662f\u4e00\u6b21\u6539\u8b8a\u4eba\u751f\u7684\u76f8\u9047\u3002\u6211\u5011\u5c07\u9996\u5148\u4ecb\u7d39\u590f\u672c\u63d0\u723e\uff0c\u5979\u662f\u6700\u521d\u5efa\u8b70\u96d9\u65b9\u5408\u4f5c\u7684\u4eba\u3002<\/p>\n

\u25cf\u590f\u672c\u63d0\u723e\u8457\u8ff7\u65bc\u75c5\u539f\u83cc<\/span><\/strong><\/p>\n

\u6709\u4eba\u66fe\u7a31\u8b9a\u590f\u672c\u63d0\u723e\u7684\u7a4d\u6975\u3001\u5c08\u5fc3\u548c\u614e\u5bc6\uff0c\u53e6\u6709\u4e9b\u4eba\u8aaa\u5979\u7e3d\u662f\u5728\u5c0b\u627e\u610f\u5916\u7684\u767c\u73fe\u3002\u5979\u81ea\u5df1\u559c\u6b61\u5f15\u7528\u8def\u6613\u2022\u5df4\u65af\u5fb7\uff08Louis Pasteur\uff09\u7684\u540d\u8a00\uff1a\u300c\u6a5f\u6703\u7e3d\u662f\u5584\u5f85\u90a3\u4e9b\u6709\u6e96\u5099\u7684\u5fc3\u9748\u300d\u3002\u8ffd\u6c42\u65b0\u767c\u73fe\u4ee5\u53ca\u5c0d\u81ea\u7531\u548c\u7368\u7acb\u7684\u6e34\u671b\uff0c\u652f\u914d\u8457\u5979\u8d70\u7684\u9053\u8def\u3002\u5305\u62ec\u5728\u5df4\u9ece\u5df4\u65af\u5fb7\u7814\u7a76\u6240\u653b\u8b80\u535a\u58eb\u5b78\u4f4d\u5728\u5167\uff0c\u5979\u66fe\u5f85\u904e\u4e94\u500b\u4e0d\u540c\u7684\u570b\u5bb6\uff0c\u4e03\u500b\u4e0d\u540c\u7684\u57ce\u5e02\uff0c\u4ee5\u53ca\u5728\u5341\u500b\u4e0d\u540c\u7684\u6a5f\u69cb\u5de5\u4f5c\u3002<\/p>\n

\u5979\u7684\u7814\u7a76\u74b0\u5883\u548c\u65b9\u6cd5\u96d6\u767c\u751f\u4e86\u8b8a\u5316\uff0c\u4f46\u662f\u5979\u7684\u5927\u90e8\u5206\u7814\u7a76\u90fd\u6709\u4e00\u500b\u5171\u540c\u5206\u6bcd\uff1a\u75c5\u539f\u83cc(pathogenic bacteria)\u3002\u5b83\u5011\u7684\u4fb5\u7565\u6027\u70ba\u4ec0\u9ebc\u90a3\u9ebc\u9ad8\uff1f\u5b83\u5011\u5982\u4f55\u767c\u5c55\u51fa\u62b5\u64cb\u6297\u751f\u7d20\u7684\u80fd\u529b\uff1f\u662f\u5426\u53ef\u4ee5\u627e\u5230\u80fd\u963b\u6b62\u5176\u9032\u5c55\u7684\u65b0\u7642\u6cd5\uff1f<\/p>\n

2002\u5e74\uff0c\u590f\u672c\u63d0\u723e\u5728\u7dad\u4e5f\u7d0d\u5927\u5b78\u6210\u7acb\u81ea\u5df1\u7684\u7814\u7a76\u5c0f\u7d44\u6642\uff0c\u5979\u5c08\u6ce8\u65bc\u5c0d\u4eba\u985e\u9020\u6210\u5371\u5bb3\u6700\u5927\u7684\u7d30\u83cc\u4e4b\u4e00\uff1a\u91c0\u81bf\u93c8\u7403\u83cc(Streptococcus pyogenes)\u3002\u5b83\u6bcf\u5e74\u611f\u67d3\u6578\u4ee5\u767e\u842c\u7684\u4eba\uff0c\u7d93\u5e38\u5f15\u8d77\u6613\u65bc\u6cbb\u7642\u7684\u611f\u67d3\uff0c\u4f8b\u5982\u6241\u6843\u817a\u708e(tonsillitis)\u548c\u81bf\u76b0\u75c7(impetigo)\uff0c\u4f46\u662f\u5b83\u4e5f\u53ef\u80fd\u5c0e\u81f4\u5371\u53ca\u751f\u547d\u7684\u6557\u8840\u75c7\uff0c\u4e26\u7834\u58de\u8eab\u9ad4\u7684\u8edf\u7d44\u7e54\uff0c\u800c\u5f97\u5230\u300c\u98df\u8089\u8005\u300d\u7684\u7a31\u865f\u3002<\/p>\n

\u70ba\u4e86\u66f4\u9032\u4e00\u6b65\u5730\u4e86\u89e3\u91c0\u81bf\u93c8\u7403\u83cc\uff0c\u590f\u672c\u63d0\u723e\u9996\u5148\u5fb9\u5e95\u7814\u7a76\u4e86\u9019\u7a2e\u7d30\u83cc\u5982\u4f55\u8abf\u63a7\u5176\u57fa\u56e0\uff0c\u9019\u6c7a\u5b9a\u662f\u767c\u73fe\u57fa\u56e0\u526a\u5200\u7684\u7b2c\u4e00\u6b65\u2013\u4f46\u662f\u5728\u6211\u5011\u9032\u4e00\u6b65\u8ddf\u96a8\u5979\u8d70\u4e0a\u9019\u689d\u8def\u4e4b\u524d\uff0c\u6211\u5011\u5c07\u5148\u4e86\u89e3\u66f4\u591a\u6709\u95dc\u9053\u7d0d\u7684\u8cc7\u8a0a\uff0c\u56e0\u70ba\u5728\u590f\u672c\u63d0\u723e\u5c0d\u91c0\u81bf\u93c8\u7403\u83cc\u9032\u884c\u8a73\u7d30\u7814\u7a76\u7684\u7576\u5152\uff0c\u9053\u7d0d\u7b2c\u4e00\u6b21\u77e5\u66c9\u4e86\u4e00\u500b\u7e2e\u5beb\uff0c\u5979\u8a8d\u70ba\u5176\u767c\u97f3\u807d\u8d77\u4f86\u5c31\u50cf\u662f\u82f1\u6587\u7684crisper\u3002<\/p>\n

\u25cf\u79d1\u5b78\u2013\u5982\u5075\u63a2\u6545\u4e8b\u4e00\u822c\u7684\u63a2\u96aa<\/strong><\/span><\/p>\n

\u5373\u4fbf\u662f\u4e00\u500b\u5728\u590f\u5a01\u5937\u9577\u5927\u7684\u5b69\u5b50\uff0c\u9053\u7d0d\u4e5f\u5f37\u70c8\u6e34\u671b\u4e86\u89e3\u5404\u7a2e\u4e8b\u7269\u3002\u4e00\u5929\uff0c\u5979\u7684\u7236\u89aa\u628a\u8a79\u59c6\u65af\u2022\u83ef\u751f\uff08James Watson\uff09\u5beb\u7684\u66f8\u300a\u96d9\u87ba\u65cb\u300b\u653e\u5728\u5979\u7684\u5e8a\u4e0a\u3002\u9019\u662f\u500b\u5177\u6709\u5075\u63a2\u98a8\u683c\u7684\u6545\u4e8b\uff0c\u63cf\u8ff0\u4e86\u83ef\u751f\u548c\u5f17\u6717\u897f\u65af\u2022\u514b\u91cc\u514b\uff08Francis Crick\uff09\u5982\u4f55\u89e3\u958b\u4e86DNA\u5206\u5b50\u7684\u7d50\u69cb\u3002\u90a3\u662f\u500b\u8207\u5979\u5728\u5b78\u6821\u6559\u79d1\u66f8\u4e2d\u6240\u8b80\u904e\u5b8c\u5168\u4e0d\u540c\u7684\u6545\u4e8b\uff0c\u5979\u88ab\u5176\u4e2d\u7684\u79d1\u5b78\u904e\u7a0b\u6240\u64c4\u7372\uff0c\u9ad4\u6703\u5230\u79d1\u5b78\u4e0d\u50c5\u50c5\u662f\u4e00\u5806\u4e8b\u5be6\u800c\u5df2\u3002<\/p>\n

\u4f46\u662f\u7576\u5979\u771f\u6b63\u958b\u59cb\u89e3\u6c7a\u79d1\u5b78\u8b0e\u5718\u6642\uff0c\u5979\u7684\u6ce8\u610f\u529b\u4e26\u975e\u653e\u5728DNA\u4e0a\uff0c\u800c\u662f\u5b83\u7684\u540c\u80de\u5206\u5b50\uff1aRNA(\u6838\u7cd6\u6838\u9178)\u3002\u7576\u6211\u5011\u57282006\u5e74\u770b\u8457\u5979\u6642\uff0c\u5979\u6b63\u5728\u52a0\u5dde\u5927\u5b78\u4f2f\u514b\u840a\u5206\u6821\u9818\u5c0e\u8457\u4e00\u500b\u7814\u7a76\u5c0f\u7d44\uff0c\u5df2\u7d93\u64c1\u6709\u4e8620\u5e74\u7684RNA\u7814\u7a76\u7d93\u9a57\uff0c\u5177\u6709\u958b\u767c\u7a81\u7834\u6027\u7814\u7a76\u7684\u9748\u654f\u55c5\u89ba\uff0c\u4e26\u7372\u5f97\u975e\u5e38\u6210\u529f\u7684\u7814\u7a76\u8072\u8b7d\uff0c\u6700\u8fd1\u525b\u9032\u5165\u4e86\u4e00\u500b\u4ee4\u4eba\u8208\u596e\u7684\u65b0\u9818\u57df\uff1aRNA\u5e72\u64fe(RNA interference)\u3002<\/p>\n

\u591a\u5e74\u4f86\u7814\u7a76\u4eba\u54e1\u4e00\u76f4\u8a8d\u70ba\u4ed6\u5011\u4e86\u89e3RNA\u7684\u57fa\u672c\u529f\u80fd\uff0c\u4f46\u4ed6\u5011\u7a81\u7136\u767c\u73fe\u8a31\u591a\u5c0f\u578bRNA (small RNA)\u53c3\u8207\u4e86\u7d30\u80de\u4e2d\u7684\u57fa\u56e0\u6d3b\u6027\u8abf\u7bc0\u3002\u9053\u7d0d\u53c3\u8207\u7684RNA\u5e72\u64fe\u7814\u7a76\uff0c\u5c0e\u81f4\u5979\u57282006\u5e74\u63a5\u5230\u4e00\u901a\u4f86\u81ea\u4e0d\u540c\u90e8\u9580\u7684\u4e00\u4f4d\u540c\u4e8b\u7684\u96fb\u8a71\u3002<\/p>\n

\u25cf\u651c\u5e36\u53e4\u8001\u514d\u75ab\u7cfb\u7d71\u7684\u7d30\u83cc<\/span><\/strong><\/p>\n

\u5979\u7684\u540c\u4e8b\u662f\u5fae\u751f\u7269\u5b78\u5bb6\uff0c\u544a\u8a34\u4e86\u9053\u7d0d\u4e00\u500b\u65b0\u767c\u73fe\uff1a\u7814\u7a76\u4eba\u54e1\u6bd4\u8f03\u5404\u7a2e\u6975\u70ba\u4e0d\u540c\u7684\u7d30\u83cc\u4ee5\u53ca\u53e4\u7d30\u83cc\uff08\u4e00\u7a2e\u5fae\u751f\u7269\uff09\u7684\u57fa\u56e0\u7269\u8cea\u6642\uff0c\u51fa\u4eba\u610f\u6599\u5730\u4ed6\u5011\u767c\u73fe\u4fdd\u5b58\u5b8c\u597d\u7684DNA\u91cd\u8907\u5e8f\u5217\uff0c\u76f8\u540c\u7684\u5bc6\u78bc\u4e00\u518d\u91cd\u8907\u51fa\u73fe\uff0c\u4f46\u662f\u5728\u91cd\u8907\u6bb5\u843d\u4e4b\u9593\u6709\u8457\u4e00\u7d44\u7368\u7279\u7684\u4e0d\u540c\u5e8f\u5217\uff08\u57162\uff09\uff0c\u5c31\u50cf\u4e00\u672c\u66f8\u4e2d\u5404\u500b\u7368\u7279\u7684\u53e5\u5b50\u4e4b\u9593\u91cd\u8907\u8457\u76f8\u540c\u7684\u55ae\u5b57\u3002<\/p>\n

\u9019\u4e9b\u91cd\u8907\u5e8f\u5217\u7684\u9663\u5217\u7a31\u70ba\u300c\u7fa4\u805a\u4e14\u6709\u898f\u5f8b\u9593\u9694\u7684\u77ed\u56de\u6587\u91cd\u8907\u5e8f\u5217\u300d\uff0c\u7e2e\u5beb\u70baCRISPR\u3002\u6709\u8da3\u7684\u662f\uff0cCRISPR\u4e2d\u7368\u7279\u7684\u975e\u91cd\u8907\u5e8f\u5217\u4f3c\u4e4e\u8207\u5404\u7a2e\u75c5\u6bd2\u7684\u57fa\u56e0\u5bc6\u78bc\u5339\u914d\uff0c\u6240\u4ee5\u76ee\u524d\u7684\u60f3\u6cd5\u70ba\uff1a\u9019\u662f\u53e4\u8001\u7684\u514d\u75ab\u7cfb\u7d71\u7684\u4e00\u90e8\u5206\uff0c\u53ef\u4ee5\u4fdd\u8b77\u7d30\u83cc\u548c\u53e4\u7d30\u83cc\u514d\u53d7\u75c5\u6bd2\u4fb5\u5bb3\u3002\u5176\u5047\u8aaa\u70ba\u5982\u679c\u7d30\u83cc\u53d7\u5230\u75c5\u6bd2\u611f\u67d3\u800c\u6210\u529f\u5730\u5b58\u6d3b\uff0c\u5b83\u5c31\u6703\u5c07\u4e00\u90e8\u5206\u75c5\u6bd2\u57fa\u56e0\u5bc6\u78bc\u52a0\u5165\u5176\u57fa\u56e0\u9ad4(genome)\uff0c\u4f5c\u70ba\u5c0d\u611f\u67d3\u7684\u8a18\u61b6\u3002<\/p>\n

\u5979\u7684\u540c\u4e8b\u8aaa\uff0c\u9084\u6c92\u6709\u4eba\u77e5\u9053\u9019\u4e00\u5207\u662f\u5982\u4f55\u904b\u4f5c\u7684\uff0c\u4f46\u61f7\u7591\u9019\u7a2e\u7d30\u83cc\u7528\u4f86\u4e2d\u548c\u75c5\u6bd2\u7684\u6a5f\u5236\uff0c\u985e\u4f3c\u65bc\u9053\u7d0d\u7814\u7a76\u7684\u8ab2\u984c\uff1aRNA\u5e72\u64fe\u3002<\/p>\n

\u25cf\u9053\u7d0d\u7e6a\u51fa\u4e86\u4e00\u500b\u8907\u96dc\u7684\u6a5f\u5668<\/span><\/strong><\/p>\n

\u9019\u500b\u6d88\u606f\u6975\u4e0d\u5c0b\u5e38\u4f46\u66f4\u4ee4\u4eba\u632f\u596e\uff0c\u5982\u679c\u7d30\u83cc\u78ba\u5be6\u5177\u6709\u4e00\u500b\u53e4\u8001\u7684\u514d\u75ab\u7cfb\u7d71\uff0c\u90a3\u9ebc\u9019\u6975\u70ba\u91cd\u8981\u3002\u9053\u7d0d\u6709\u8457\u4e00\u7a2e\u6829\u6829\u5982\u751f\u7684\u5206\u5b50\u8a08\u8b00\u611f\u89ba\uff0c\u5979\u958b\u59cb\u5b78\u7fd2\u6709\u95dcCRISPR\u7cfb\u7d71\u7684\u6240\u6709\u77e5\u8b58\u3002<\/p>\n

\u7814\u7a76\u4eba\u54e1\u8b49\u660e\u9664\u4e86CRISPR\u5e8f\u5217\u5916\uff0c\u9084\u767c\u73fe\u4e86\u4e00\u4e9b\u7279\u6b8a\u57fa\u56e0\uff0c\u4ed6\u5011\u5c07\u5176\u7a31\u70baCRISPR-\u95dc\u806f\u8005(CRISPR-associated)\uff0c\u7c21\u7a31\u70bacas\u3002\u6709\u8da3\u7684\u662f\u9053\u7d0d\u767c\u73fe\u9019\u4e9b\u57fa\u56e0\u7684\u7de8\u78bc\uff0c\u8207\u5df2\u77e5\u5c08\u9580\u7528\u65bc\u9b06\u89e3\u548c\u5207\u5272DNA\u7684\u86cb\u767d\u8cea\u4e4b\u57fa\u56e0\u975e\u5e38\u76f8\u4f3c\u3002\u90a3\u9ebcCas\u86cb\u767d\u8cea\u5177\u6709\u76f8\u540c\u7684\u529f\u80fd\u55ce\uff1f\u5b83\u5011\u6703\u5207\u5272\u75c5\u6bd2DNA\u55ce\uff1f<\/p>\n

\u5979\u8b93\u5979\u7684\u7814\u7a76\u5c0f\u7d44\u958b\u59cb\u5de5\u4f5c\uff0c\u5e7e\u5e74\u5f8c\uff0c\u5979\u5011\u6210\u529f\u5730\u63ed\u793a\u4e86\u5e7e\u7a2e\u4e0d\u540cCas\u86cb\u767d\u8cea\u7684\u529f\u80fd\u3002\u540c\u6642\uff0c\u5c11\u6578\u5e7e\u500b\u5176\u5b83\u5927\u5b78\u7684\u7814\u7a76\u5c0f\u7d44\uff0c\u4e5f\u6b63\u5728\u7814\u7a76\u65b0\u767c\u73fe\u7684CRISPR\/Cas\u7cfb\u7d71\u3002\u4ed6\u5011\u6240\u7e6a\u51fa\u7684\u5716\u50cf\u986f\u793a\u7d30\u83cc\u7684\u514d\u75ab\u7cfb\u7d71\u53ef\u4ee5\u63a1\u53d6\u975e\u5e38\u4e0d\u540c\u7684\u5f62\u5f0f\uff0c\u9053\u7d0d\u6240\u7814\u7a76\u7684CRISPR\/Cas\u7cfb\u7d71\u5c6c\u65bc\u7b2c1\u985e\uff1b\u90a3\u662f\u4e00\u500b\u8907\u96dc\u7684\u6a5f\u5668\uff0c\u9700\u8981\u8a31\u591a\u4e0d\u540c\u7684Cas\u86cb\u767d\u8cea\u4f86\u6e05\u9664\u75c5\u6bd2\u3002\u7b2c2\u985e\u7cfb\u7d71\u76f8\u7576\u7c21\u55ae\uff0c\u56e0\u70ba\u5b83\u5011\u9700\u8981\u8f03\u5c11\u7684\u86cb\u767d\u8cea\u3002\u5728\u4e16\u754c\u7684\u53e6\u4e00\u7aef\uff0c\u590f\u672c\u63d0\u723e\u525b\u525b\u9047\u5230\u4e86\u9019\u6a23\u7684\u7cfb\u7d71\uff0c\u73fe\u5728\u5c31\u8b93\u6211\u5011\u7684\u6545\u4e8b\u56de\u5230\u5979\u8eab\u908a\u3002<\/p>\n

\u25cfCRISPR\u7cfb\u7d71\u4e2d\u4e00\u7247\u65b0\u800c\u672a\u77e5\u7684\u62fc\u5716\u584a<\/strong><\/span><\/p>\n

\u7576\u6211\u5011\u7684\u6545\u4e8b\u96e2\u958b\u590f\u672c\u63d0\u723e\u6642\uff0c\u5979\u6b63\u4f4f\u5728\u7dad\u4e5f\u7d0d\uff0c\u4f46\u57282009\u5e74\uff0c\u5979\u7372\u5f97\u4e86\u5f88\u597d\u7684\u7814\u7a76\u6a5f\u6703\u800c\u642c\u5230\u4e86\u5728\u745e\u5178\u5317\u90e8\u7684\u65bc\u9ed8\u5967\u5927\u5b78\uff08Ume\u00e5 University\uff09\u3002\u6709\u4eba\u8b66\u544a\u5979\u8981\u642c\u5230\u4e16\u754c\u4e0a\u5982\u6b64\u504f\u9060\u7684\u5730\u5340\u4e0d\u662f\u500b\u597d\u6c7a\u5b9a\uff0c\u4f46\u662f\u6f2b\u9577\u800c\u9ed1\u6697\u7684\u51ac\u5929\u4f7f\u5979\u5de5\u4f5c\u6642\u5145\u6eff\u4e86\u5e73\u548c\u8207\u5be7\u975c\u3002<\/p>\n

\u9019\u6b63\u662f\u5979\u9700\u8981\u7684\u3002\u5979\u6b63\u597d\u4e5f\u5c0d\u8abf\u63a7\u57fa\u56e0\u7684\u5c0f\u578bRNA\u611f\u8208\u8da3\uff0c\u4e26\u8207\u5728\u67cf\u6797\u7684\u7814\u7a76\u4eba\u54e1\u5408\u4f5c\uff0c\u8a73\u7d30\u7814\u7a76\u4e86\u91c0\u81bf\u93c8\u7403\u83cc\u4e2d\u767c\u73fe\u7684\u4e00\u4e9b\u5c0f\u578bRNA\u3002\u7814\u7a76\u7684\u7d50\u679c\u8b93\u5979\u82b1\u4e86\u8a31\u591a\u6642\u9593\u601d\u8003\uff0c\u56e0\u70ba\u8a72\u7d30\u83cc\u4e2d\u4e00\u7a2e\u5927\u91cf\u5b58\u5728\u7684\u5c0f\u578bRNA\u662f\u4e00\u500b\u65b0\u7684\u8b8a\u7a2e\uff0c\u8a72RNA\u7684\u57fa\u56e0\u5bc6\u78bc\u8207\u8a72\u7d30\u83cc\u57fa\u56e0\u9ad4\u4e2d\u5947\u7279\u7684CRISPR\u5e8f\u5217\u975e\u5e38\u985e\u4f3c\u3002<\/p>\n

\u5169\u8005\u4e4b\u9593\u7684\u76f8\u4f3c\u6027\u8b93\u590f\u672c\u63d0\u723e\u61f7\u7591\u5b83\u5011\u662f\u6709\u95dc\u806f\u7684\uff0c\u4ed4\u7d30\u5206\u6790\u5b83\u5011\u7684\u57fa\u56e0\u5bc6\u78bc\u9084\u986f\u793a\uff0c\u672a\u77e5\u7684\u5c0f\u578bRNA\u7684\u4e00\u90e8\u5206\u8207\u91cd\u8907\u7684CRISPR\u76f8\u4e92\u5339\u914d\uff0c\u5c31\u50cf\u627e\u5230\u5169\u584a\u5b8c\u5168\u5bc6\u5408\u7684\u62fc\u5716\u584a\u4e00\u6a23\uff08\u57162\uff09\u3002<\/p>\n

\u590f\u672c\u63d0\u723e\u5f9e\u672a\u7814\u7a76\u904eCRISPR\uff0c\u4f46\u5979\u7684\u7814\u7a76\u5c0f\u7d44\u70ba\u4e86\u91d0\u6e05\u91c0\u81bf\u93c8\u7403\u83cc\u4e2d\u7684CRISPR\u7cfb\u7d71\uff0c\u555f\u52d5\u4e86\u4e00\u4e9b\u5fb9\u5e95\u7684\u5fae\u751f\u7269\u5b78\u5075\u63a2\u5de5\u4f5c\u3002\u9019\u500b\u7cfb\u7d71\uff0c\u5c6c\u65bc\u5df2\u77e5\u7684\u7b2c2\u985e\uff0c\u50c5\u9700\u4e00\u500bCas\u86cb\u767d\u8cea\uff1aCas9\uff0c\u5373\u53ef\u88c2\u89e3\u75c5\u6bd2DNA\u3002\u590f\u672c\u63d0\u723e\u767c\u73fe\uff0c\u88ab\u7a31\u70ba\u8b04\u672c\u6d3b\u5316crispr RNA\uff08tracrRNA\uff09\u7684\u65b0RNA\u5206\u5b50\uff0c\u4e5f\u5177\u6709\u6c7a\u5b9a\u6027\u529f\u80fd\uff1b\u57fa\u56e0\u9ad4\u4e2d\u7684CRISPR\u5e8f\u5217\u6240\u88fd\u9020\u51fa\u4f86\u7684\u9577\u6bb5RNA\uff0c\u9700\u8981tracrRNA\u8b93\u5b83\u6210\u719f\u70ba\u6d3b\u6027\u5f62\u5f0f\uff08\u57162\uff09\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u7d93\u904e\u6df1\u5165\u800c\u6709\u76ee\u6a19\u6027\u7684\u5be6\u9a57\uff0c\u590f\u672c\u63d0\u723e\u65bc2011\u5e743\u6708\u767c\u8868\u4e86tracrRNA\u7684\u767c\u73fe\uff0c\u5979\u77e5\u9053\u81ea\u5df1\u6b63\u5c3e\u96a8\u8457\u67d0\u500b\u7cbe\u91c7\u7684\u6771\u897f\u3002\u5979\u6709\u591a\u5e74\u7684\u5fae\u751f\u7269\u5b78\u7d93\u9a57\uff0c\u4f46\u70ba\u4e86\u6301\u7e8c\u7814\u7a76CRISPR-Cas9\u7684\u7cfb\u7d71\uff0c\u5979\u5e0c\u671b\u80fd\u8207\u751f\u7269\u5316\u5b78\u5bb6\u5408\u4f5c\uff0c\u9053\u7d0d\u5247\u662f\u7576\u7136\u7684\u9078\u64c7\u3002\u56e0\u6b64\u5728\u7576\u5e74\u6625\u5929\uff0c\u590f\u672c\u63d0\u723e\u53d7\u9080\u53c3\u52a0\u5728\u6ce2\u591a\u9ece\u5404\u8209\u884c\u7684\u6703\u8b70\u5831\u544a\u5979\u7684\u767c\u73fe\u6642\uff0c\u5979\u4f01\u5716\u7d50\u8b58\u9019\u4f4d\u975e\u5e38\u6709\u7d93\u9a57\u7684\u4f2f\u514b\u840a\u7814\u7a76\u4eba\u54e1\u3002<\/p>\n

\u25cf\u6ce2\u591a\u9ece\u5404<\/strong>\u5496\u5561\u9928\u4e2d\u6539\u8b8a\u4eba\u751f\u7684\u6703\u9762<\/strong><\/span><\/p>\n

\u78b0\u5de7\u7684\u662f\uff0c\u5979\u5011\u5728\u6703\u8b70\u7684\u7b2c\u4e8c\u5929\u5728\u4e00\u5bb6\u5496\u5561\u9928\u898b\u4e86\u9762\uff0c\u9053\u7d0d\u7684\u540c\u4e8b\u4ecb\u7d39\u4e86\u5979\u5011\u8a8d\u8b58\u3002\u7b2c\u4e8c\u5929\uff0c\u590f\u672c\u63d0\u723e\u63d0\u8b70\u5979\u5011\u61c9\u8a72\u4e00\u8d77\u63a2\u7d22\u9019\u500b\u9996\u90fd\u7684\u820a\u57ce\u5340\u3002\u7576\u5979\u5011\u6cbf\u8457\u9d5d\u5375\u77f3\u92ea\u5c31\u7684\u8857\u9053\u6f2b\u6b65\u6642\uff0c\u958b\u59cb\u8ac7\u5230\u5f7c\u6b64\u7684\u7814\u7a76\uff0c\u590f\u672c\u63d0\u723e\u60f3\u77e5\u9053\u9053\u7d0d\u662f\u5426\u5c0d\u5408\u4f5c\u611f\u8208\u8da3\uff1f\u5979\u6709\u610f\u53c3\u8207\u91c0\u81bf\u93c8\u7403\u83cc\u7c21\u55ae\u7684\u7b2c2\u985e\u7cfb\u7d71\u4e2d\uff0cCas9\u529f\u80fd\u7684\u7814\u7a76\u55ce\uff1f<\/p>\n

\u9053\u7d0d\u5f88\u611f\u8208\u8da3\uff0c\u5979\u5011\u548c\u540c\u4e8b\u5011\u901a\u904e\u7dda\u4e0a\u6703\u8b70\u64ec\u5b9a\u4e86\u8a72\u7814\u7a76\u7684\u8a08\u5283\u3002\u5979\u5011\u61f7\u7591CRISPR-RNA\u662f\u8fa8\u8b58\u75c5\u6bd2DNA\u6240\u5fc5\u9700\u7684\uff0c\u800cCas9\u662f\u5207\u65b7DNA\u5206\u5b50\u7684\u526a\u5200\u3002\u4f46\u662f\u7576\u5979\u5011\u5728\u751f\u7269\u9ad4\u5916\u9032\u884c\u6e2c\u8a66\u6642\uff0c\u4ec0\u9ebc\u90fd\u6c92\u6709\u767c\u751f\uff0cDNA\u5206\u5b50\u4ecd\u4fdd\u6301\u5b8c\u6574\u3002\u70ba\u4ec0\u9ebc\uff1f\u5be6\u9a57\u689d\u4ef6\u6709\u554f\u984c\u55ce\uff1f\u9084\u662f\u8aaaCas9\u5177\u6709\u5b8c\u5168\u4e0d\u540c\u7684\u529f\u80fd\uff1f<\/p>\n

\u7d93\u904e\u5927\u91cf\u7684\u8166\u529b\u6fc0\u76ea\u548c\u7121\u6578\u5931\u6557\u7684\u5be6\u9a57\u4e4b\u5f8c\uff0c\u5979\u5011\u7684\u7814\u7a76\u4eba\u54e1\u7d42\u65bc\u6dfb\u52a0\u4e86tracrRNA\u9032\u884c\u6e2c\u8a66\uff0c\u539f\u5148\u4ed6\u5011\u8a8d\u70ba\u50c5\u5728CRISPR RNA\u88ab\u5207\u5272\u6210\u6d3b\u6027\u5f62\u5f0f\u5f8c\uff0c\u624d\u9700\u8981tracrRNA\uff08\u57162\uff09\uff0c\u4f46\u662f\u4e00\u65e6Cas9\u53ef\u4ee5\u4f7f\u7528tracrRNA\uff0c\u5927\u5bb6\u4e00\u76f4\u671f\u5f85\u7684\u4e8b\u60c5\u771f\u7684\u767c\u751f\u4e86\uff1aDNA\u5206\u5b50\u88ab\u5207\u5272\u6210\u5169\u90e8\u5206\u3002<\/p>\n

\u5927\u81ea\u7136\u900f\u904e\u9032\u5316\u89e3\u6c7a\u554f\u984c\u7684\u65b9\u6cd5\u7d93\u5e38\u4f7f\u7814\u7a76\u4eba\u54e1\u611f\u5230\u9a5a\u8a1d\uff0c\u4f46\u9019\u6b21\u537b\u662f\u975e\u540c\u5c0b\u5e38\u7684\uff0c\u93c8\u7403\u83cc\u6240\u767c\u5c55\u51fa\u7684\u62b5\u79a6\u75c5\u6bd2\u6b66\u5668\uff0c\u65e2\u7c21\u55ae\u53c8\u6709\u6548\uff0c\u751a\u81f3\u53ef\u8b02\u5091\u51fa\u3002\u57fa\u56e0\u526a\u5200\u7684\u6b77\u53f2\u53ef\u80fd\u5c31\u6b64\u7d50\u675f\uff1a\u590f\u672c\u63d0\u723e\u548c\u9053\u7d0d\u66fe\u7d93\u5728\u4e00\u7a2e\u5c0d\u4eba\u985e\u9020\u6210\u5de8\u5927\u82e6\u96e3\u7684\u7d30\u83cc\u4e2d\uff0c\u767c\u73fe\u4e86\u4e00\u7a2e\u7d30\u83cc\u7684\u57fa\u672c\u6a5f\u5236\u3002\u5149\u662f\u9019\u767c\u73fe\u5c31\u5df2\u4ee4\u4eba\u9a5a\u5947\uff0c\u4f46\u6a5f\u6703\u7e3d\u662f\u5584\u5f85\u90a3\u4e9b\u6709\u6e96\u5099\u7684\u5fc3\u9748\u3002<\/p>\n

\u25cf\u5283\u6642\u4ee3\u7684\u5be6\u9a57<\/strong><\/span><\/p>\n

\u7814\u7a76\u5c0f\u7d44\u4eba\u54e1\u6c7a\u5b9a\u5617\u8a66\u7c21\u5316\u57fa\u56e0\u526a\u5200\uff0c\u5229\u7528\u5c0dtracrRNA\u548cCRISPR-RNA\u7684\u65b0\u77e5\u8b58\uff0c\u4ed6\u5011\u8a2d\u8a08\u51fa\u65b9\u6cd5\u5c07\u5169\u8005\u878d\u5408\u6210\u4e00\u500b\u5206\u5b50\uff0c\u547d\u540d\u70ba\u300c\u56ae\u5c0eRNA\u300d(guide RNA)\u3002\u5229\u7528\u9019\u7a2e\u7c21\u5316\u7684\u57fa\u56e0\u526a\u5200\u8b8a\u9ad4\uff0c\u4ed6\u5011\u63a5\u8457\u958b\u59cb\u9032\u884c\u5283\u6642\u4ee3\u7684\u5be6\u9a57\uff1a\u7814\u7a76\u662f\u5426\u53ef\u4ee5\u63a7\u5236\u9019\u7a2e\u57fa\u56e0\u5de5\u5177\uff0c\u5f9e\u800c\u65bc\u7814\u7a76\u4eba\u54e1\u6307\u5b9a\u7684\u4f4d\u7f6e\u5207\u5272DNA\u3002<\/p>\n

\u6b64\u6642\u7814\u7a76\u4eba\u54e1\u77e5\u9053\u5df2\u7d93\u63a5\u8fd1\u4e86\u91cd\u5927\u7a81\u7834\uff0c\u4ed6\u5011\u4f7f\u7528\u4e00\u500b\u5df2\u7d93\u5728\u9053\u7d0d\u5be6\u9a57\u5ba4\u7684\u51b0\u6ac3\u4e2d\u5b58\u653e\u7684\u57fa\u56e0\uff0c\u4e26\u9078\u64c7\u4e86\u4e94\u500b\u4e0d\u540c\u7684\u57fa\u56e0\u5207\u5272\u4f4d\u7f6e\uff0c\u7136\u5f8c\u66f4\u6539\u526a\u5200\u7684CRISPR\u90e8\u5206\uff0c\u4f7f\u5176\u5bc6\u78bc\u8207\u9032\u884c\u5207\u5272\u4f4d\u7f6e\u7684\u5bc6\u78bc\u5339\u914d\uff08\u57163\uff09\uff0c\u7d50\u679c\u662f\u52e2\u4e0d\u53ef\u64cb\u7684\u5c07DNA\u5206\u5b50\u5207\u5272\u5728\u6b63\u78ba\u7684\u4f4d\u7f6e\u3002<\/p>\n

\u25cf\u57fa\u56e0\u526a\u5200\u6539\u8b8a\u4e86\u751f\u547d\u79d1\u5b78<\/strong><\/span><\/p>\n

\u590f\u672c\u63d0\u723e\u548c\u9053\u7d0d\u57282012\u5e74\u767c\u8868CRISPR\/Cas9\u57fa\u56e0\u526a\u5200\u7684\u767c\u73fe\u5f8c\u4e0d\u4e45\uff0c\u9019\u500b\u5de5\u5177\u88ab\u591a\u500b\u7814\u7a76\u5c0f\u7d44\u5c55\u793a\u53ef\u7528\u65bc\u4fee\u98fe\u5c0f\u9f20\u548c\u4eba\u985e\u7d30\u80de\u4e2d\u7684\u57fa\u56e0\u9ad4\uff0c\u5c0e\u81f4\u7206\u70b8\u6027\u7684\u767c\u5c55\u3002\u5148\u524d\uff0c\u6539\u8b8a\u7d30\u80de\u3001\u690d\u7269\u6216\u751f\u7269\u9ad4\u4e2d\u7684\u57fa\u56e0\u975e\u5e38\u8017\u6642\uff0c\u6709\u6642\u751a\u81f3\u662f\u4e0d\u53ef\u80fd\u7684\u3002\u4f7f\u7528\u9019\u7a2e\u57fa\u56e0\u526a\u5200\uff0c\u7814\u7a76\u4eba\u54e1\u539f\u5247\u4e0a\u53ef\u4ee5\u5728\u4ed6\u5011\u60f3\u7528\u7684\u4efb\u4f55\u57fa\u56e0\u9ad4\u4e2d\u9032\u884c\u5207\u5272\u3002\u63a5\u8457\u53ef\u4ee5\u5f88\u5bb9\u6613\u5229\u7528\u7d30\u80de\u7684\u5929\u7136DNA\u4fee\u5fa9\u7cfb\u7d71\uff0c\u5f9e\u800c\u6539\u5beb\u751f\u547d\u5bc6\u78bc\uff08\u57163\uff09\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u7531\u65bc\u9019\u7a2e\u57fa\u56e0\u5de5\u5177\u975e\u5e38\u6613\u65bc\u4f7f\u7528\uff0c\u73fe\u5728\u5ee3\u6cdb\u7684\u61c9\u7528\u65bc\u57fa\u790e\u7814\u7a76\u4e2d\u3002\u5b83\u53ef\u7528\u65bc\u66f4\u6539\u7d30\u80de\u548c\u5be6\u9a57\u52d5\u7269\u7684DNA\uff0c\u4ee5\u4e86\u89e3\u4e0d\u540c\u57fa\u56e0\u7684\u529f\u80fd\u548c\u76f8\u4e92\u7684\u4f5c\u7528\uff0c\u4f8b\u5982\u5728\u75be\u75c5\u904e\u7a0b\u4e2d\u7684\u89d2\u8272\u3002<\/p>\n

\u57fa\u56e0\u526a\u5200\u4e5f\u5df2\u6210\u70ba\u690d\u7269\u80b2\u7a2e\u7684\u6a19\u6e96\u5de5\u5177\uff0c\u4ee5\u524d\u7814\u7a76\u4eba\u54e1\u4fee\u6539\u690d\u7269\u57fa\u56e0\u9ad4\u4f7f\u7528\u7684\u65b9\u6cd5\uff0c\u901a\u5e38\u9700\u8981\u6dfb\u52a0\u6297\u751f\u7d20\u8010\u85e5\u6027\u57fa\u56e0\uff0c\u4f46\u7a2e\u690d\u9019\u7a2e\u8fb2\u4f5c\u7269\u6642\uff0c\u5176\u6297\u751f\u7d20\u8010\u85e5\u6027\u53ef\u80fd\u6703\u64f4\u6563\u7d66\u5468\u570d\u5fae\u751f\u7269\u3002\u591a\u8667\u4e86\u57fa\u56e0\u526a\u5200\uff0c\u7814\u7a76\u4eba\u54e1\u4e0d\u518d\u9700\u8981\u4f7f\u7528\u9019\u4e9b\u8f03\u820a\u7684\u65b9\u6cd5\uff0c\u56e0\u70ba\u4ed6\u5011\u73fe\u5728\u53ef\u4ee5\u5c0d\u57fa\u56e0\u9ad4\u9032\u884c\u975e\u5e38\u7cbe\u78ba\u7684\u66f4\u6539\u3002\u8a31\u591a\u5176\u5b83\u61c9\u7528\u4e2d\uff0c\u4ed6\u5011\u9084\u7de8\u8f2f\u4e86\u4f7f\u6c34\u7a3b\u5438\u6536\u571f\u58e4\u4e2d\u91cd\u91d1\u5c6c\u7684\u57fa\u56e0\uff0c\u4f7f\u5f97\u6539\u826f\u7684\u6c34\u7a3b\u54c1\u7a2e\u5177\u6709\u8f03\u4f4e\u7684\u9398\u548c\u7837\u7684\u542b\u91cf\u3002\u7814\u7a76\u4eba\u54e1\u9084\u958b\u767c\u4e86\u5728\u6eab\u6696\u7684\u6c23\u5019\u4e2d\u66f4\u80fd\u62b5\u6297\u4e7e\u65f1\u7684\u8fb2\u4f5c\u7269\uff0c\u4e26\u4e14\u53ef\u4ee5\u62b5\u6297\u6606\u87f2\u548c\u5bb3\u87f2\uff0c\u5426\u5247\u5c07\u4e0d\u5f97\u4e0d\u4f7f\u7528\u8fb2\u85e5\u3002<\/p>\n

\u25cf\u907a\u50b3\u75be\u75c5\u6cbb<\/strong>\u7652\u7684\u5e0c\u671b<\/strong><\/span><\/p>\n

\u5728\u91ab\u5b78\u4e0a\uff0c\u57fa\u56e0\u526a\u5200\u70ba\u764c\u75c7\u7684\u65b0\u514d\u75ab\u7642\u6cd5\u505a\u51fa\u4e86\u8ca2\u737b\uff0c\u5be6\u9a57\u6b63\u5728\u4f01\u5716\u8b93\u5922\u60f3\u5be6\u73fe\u2013\u6cbb\u7652\u907a\u50b3\u75be\u75c5\u3002\u7814\u7a76\u4eba\u54e1\u5df2\u7d93\u5728\u9032\u884c\u81e8\u5e8a\u8a66\u9a57\u4ee5\u7814\u7a76\u662f\u5426\u53ef\u4ee5\u4f7f\u7528CRISPR\/Cas9\u4f86\u6cbb\u7642\u4e00\u4e9b\u8840\u6db2\u75be\u75c5\uff0c\u4f8b\u5982\u942e\u5f62\u8840\u7403\u8ca7\u8840\u75c7\u548cbeta-\u5730\u4e2d\u6d77\u578b\u8ca7\u8840\u75c7\uff0c\u4ee5\u53ca\u907a\u50b3\u6027\u773c\u75be\u3002<\/p>\n

\u4ed6\u5011\u4e5f\u5728\u958b\u767c\u4fee\u5fa9\u5927\u578b\u5668\u5b98\u4e2d\u57fa\u56e0\u7684\u65b9\u6cd5\uff0c\u4f8b\u5982\u5927\u8166\u548c\u808c\u8089\u3002\u52d5\u7269\u5be6\u9a57\u986f\u793a\uff0c\u7d93\u904e\u7279\u6b8a\u8a2d\u8a08\u7684\u75c5\u6bd2\u53ef\u4ee5\u5c07\u57fa\u56e0\u526a\u5200\u5c0e\u5165\u9700\u8981\u7684\u7d30\u80de\uff0c\u4f5c\u70ba\u6cbb\u7642\u6bc0\u6ec5\u6027\u907a\u50b3\u75be\u75c5\u7684\u6a21\u578b\uff0c\u4f8b\u5982\u808c\u8089\u5931\u990a\u75c7\uff0c\u810a\u9ad3\u808c\u8089\u840e\u7e2e\u548c\u676d\u4e01\u9813\u821e\u8e48\u75c7\u3002\u4f46\u662f\u8a72\u6280\u8853\u4ecd\u9700\u8981\u9032\u4e00\u6b65\u6539\u5584\uff0c\u4f7f\u4e4b\u80fd\u5728\u4eba\u9ad4\u4e0a\u9032\u884c\u6e2c\u8a66\u3002<\/p>\n

\u25cf\u57fa\u56e0\u526a\u5200\u7684\u80fd\u529b\u9700\u8981\u76e3\u63a7<\/strong><\/span><\/p>\n

\u8207\u5176\u6240\u6709\u512a\u9ede\u4e26\u5b58\u7684\uff0c\u57fa\u56e0\u526a\u5200\u4e5f\u53ef\u80fd\u88ab\u6feb\u7528\u3002\u4f8b\u5982\uff0c\u53ef\u4ee5\u4f7f\u7528\u6b64\u5de5\u5177\u5275\u9020\u57fa\u56e0\u4fee\u98fe\u904e\u7684\u80da\u80ce\u3002\u4f46\u662f\uff0c\u591a\u5e74\u4f86\u6709\u6cd5\u5f8b\u53ca\u6cd5\u898f\u63a7\u5236\u57fa\u56e0\u5de5\u7a0b\u7684\u61c9\u7528\uff0c\u5176\u4e2d\u5305\u62ec\u7981\u6b62\u6703\u8b93\u8b8a\u5316\u907a\u50b3\u7d66\u5f8c\u4ee3\u7684\u4eba\u985e\u57fa\u56e0\u9ad4\u4fee\u6539\u65b9\u5f0f\u3002\u53e6\u5916\uff0c\u6d89\u53ca\u4eba\u985e\u548c\u52d5\u7269\u7684\u5be6\u9a57\uff0c\u5fc5\u9808\u7d93\u904e\u9053\u5fb7\u59d4\u54e1\u6703\u7684\u5be9\u67e5\u548c\u6279\u51c6\u624d\u5f97\u57f7\u884c\u3002<\/p>\n

\u53ef\u4ee5\u80af\u5b9a\u7684\u662f\uff1a\u9019\u4e9b\u57fa\u56e0\u526a\u5200\u5f71\u97ff\u8457\u6211\u5011\u6240\u6709\u4eba\u3002\u6211\u5011\u5c07\u9762\u81e8\u65b0\u7684\u502b\u7406\u554f\u984c\uff0c\u4f46\u662f\u6b64\u4e00\u65b0\u7684\u5de5\u5177\u5f88\u53ef\u80fd\u6709\u52a9\u65bc\u89e3\u6c7a\u7576\u4eca\u4eba\u985e\u9762\u81e8\u7684\u8a31\u591a\u6311\u6230\u3002\u901a\u904e\u5979\u5011\u7684\u767c\u73fe\uff0c\u590f\u672c\u63d0\u723e\u548c\u9053\u7d0d\u958b\u767c\u4e86\u4e00\u7a2e\u5316\u5b78\u5de5\u5177\uff0c\u5c07\u751f\u547d\u79d1\u5b78\u5e36\u5165\u4e86\u4e00\u500b\u65b0\u6642\u4ee3\u3002\u5979\u5011\u4f7f\u6211\u5011\u6ce8\u8996\u8457\u7121\u6cd5\u60f3\u50cf\u7684\u5ee3\u95ca\u524d\u666f\uff0c\u4e26\u4e14\uff0c\u5728\u6211\u5011\u63a2\u7d22\u9019\u7247\u65b0\u9818\u57df\u7684\u904e\u7a0b\u4e2d\uff0c\u4e00\u5b9a\u6703\u5275\u9020\u51fa\u65b0\u7684\u3001\u610f\u60f3\u4e0d\u5230\u7684\u767c\u73fe\u3002<\/p>\n

 <\/p>\n

 <\/p>\n

--
\n\u672c\u6587\u8b6f\u81ea\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u59d4\u54e1\u6703\u516c\u4f48\u7d66\u5927\u773e\u7684\u65b0\u805e\u7a3f\uff0c\u539f\u6587\u53ef\u81ea\u4ee5\u4e0b\u5b98\u65b9\u7db2\u7ad9\u53d6\u5f97\uff1a
\nhttps:\/\/www.nobelprize.org\/uploads\/2020\/10\/popular-chemistryprize2020.pdf<\/a><\/p>\n

\u82e5\u6709\u8208\u8da3\u95b1\u8b80\u9032\u968e\u7684\u539f\u6587\u8cc7\u6599\uff0c\u8acb\u7531\u4e0b\u5217\u5b98\u65b9\u7db2\u5740\u53d6\u5f97\uff1a
\nhttps:\/\/www.nobelprize.org\/uploads\/2020\/10\/advanced-chemistryprize2020.pdf<\/a><\/p>\n

 <\/p>\n

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