{"id":35557,"date":"2020-08-17T00:11:48","date_gmt":"2020-08-16T16:11:48","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=35557"},"modified":"2020-10-23T12:49:07","modified_gmt":"2020-10-23T04:49:07","slug":"%e3%80%90%e9%97%9c%e6%96%bc-covid-19-%e7%9a%84%e9%80%99%e5%a4%a7%e5%8d%8a%e5%b9%b4%e3%80%91%e6%aa%a2%e9%a9%97%e4%b8%8a%e7%9a%84%e5%85%a9%e4%b8%89%e4%ba%8b","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=35557","title":{"rendered":"\u3010\u95dc\u65bc COVID-19 \u7684\u9019\u5927\u534a\u5e74\u3011\u6aa2\u9a57\u4e0a\u7684\u5169\u4e09\u4e8b"},"content":{"rendered":"

\u8f49\u773c\u9593\uff0c2020 \u5e74\u590f\u5929\u5c31\u9019\u6a23\u4f86\u4e86\u3002\u5927\u534a\u5e74\u88e1\uff0c\u300c\u56b4\u91cd\u7279\u6b8a\u50b3\u67d3\u6027\u80ba\u708e\u300dCOVID-19 \uff08\u4ea6\u7a31\u6b66\u6f22\u80ba\u708e\uff09\u7684\u75ab\u60c5\u5e2d\u6372\u5168\u7403\uff0c\u5982\u4eca\u4f9d\u7136\u5f35\u72c2\u3002\u800c\u53f0\u7063\uff0c\u50cf\u500b\u4e16\u5916\u6843\u6e90\u3002\u63a5\u4e0b\u4f86\uff0c\u5728\u9019\u4e00\u7cfb\u5217\u5c08\u984c\u88e1\uff0c\u6211\u5011\u5c31\u4e00\u8d77\u4f86\u8a0e\u8ad6\u9019\u5927\u534a\u5e74\u88e1\uff0c\u5728\u81e8\u5e8a\u8207\u7814\u7a76\u524d\u7dda\u4e0a\uff0c\u6211\u5011\u5c0d\u65bc\u9019\u60e1\u540d\u662d\u5f70\u7684 COVID-19 \u8207\u7f6a\u9b41\u798d\u9996 SARS-CoV-2\uff0c\u5404\u754c\u5c08\u5bb6\u7aae\u76e1\u5fc3\u529b\u6240\u6162\u6162\u641e\u6e05\u695a\uff08\u6216\u662f\u9084\u6c92\u5f04\u660e\u767d\uff09\u5730\u9019\u4e9b\u90a3\u4e9b\u4e8b\u5427\u3002\u8b93\u6211\u5011\u4e00\u8d77\u4f86\u4ee5\u75ab\u60c5\u70ba\u57fa\uff0c\u597d\u597d\u5730\u8a0e\u8ad6\u514d\u75ab\u5b78\u5427\uff01<\/strong><\/span><\/p>\n

\"\"
\u5716\u7247\u4f86\u6e90:Wiki<\/figcaption><\/figure>\n

\u64b0\u6587\uff0f\u99f1\u5b9b\u7433<\/strong><\/p>\n

\u9996\u5148\uff0c\u5728\u958b\u59cb\u8a0e\u8ad6\u75c5\u6bd2\u7279\u6027\u3001\u514d\u75ab\u53cd\u61c9\u3001\u75ab\u82d7\u9032\u5c55\u3001\u8207\u85e5\u7269\u958b\u767c\u4e4b\u524d\uff0c\u6211\u5011\u5148\u4f86\u804a\u804a\u95dc\u65bc SARS-CoV-2 \u75c5\u6bd2\u6aa2\u6e2c\u4e0a\u7684\u4e00\u4e9b\u4e8b\u60c5\u3002<\/p>\n

\u76ee\u524d\uff0c\u6700\u5e38\u88ab\u8a0e\u8ad6\u7528\u4f86\u6aa2\u6e2c SARS-CoV-2 \u75c5\u6bd2\u7684\u5169\u7a2e\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u5206\u5225\u662f\u4ee5\u5206\u5b50\u751f\u7269\u5b78\u6838\u9178\u70ba\u672c\u7684 RT-qPCR \u8a66\u9a57\uff0c\u4ee5\u53ca\u8840\u6e05\u5b78\u6aa2\u6e2c\uff08serological test\uff09\u3002\u5169\u7a2e\u6e2c\u9a57\u80fd\u900f\u9732\u7684\u8a0a\u606f\u4e0d\u592a\u4e00\u6a23\u3002RT-qPCR \u70ba\u4e3b\u7684\u6aa2\u6e2c\uff0c\u65e8\u5728\u628a SARS-CoV-2 \u9019\u73fe\u884c\u72af\uff0c\u5728\u72af\u7f6a\u73fe\u5834\u6293\u500b\u6b63\u8457\u3002\u800c\u8840\u6e05\u5b78\u6aa2\u6e2c\uff0c\u5247\u5f88\u50cf\u662f\u53bb\u8b66\u8abf\u55ae\u4f4d\u53bb\u63a2\u67e5 SARS-CoV-2 \u7684\u904e\u5f80\u72af\u7f6a\u53f2\u3002<\/p>\n

RT-qPCR \u6aa2\u6e2c\u5e38\u5e38\u88ab\u7c21\u7a31\u70baPCR\uff0c\u96d6\u7136\u4ee5\u79d1\u5b78\u56b4\u8b39\u5ea6\u4f86\u8aaa\uff0c\u9019\u7a2e\u7c21\u7a31\u4e0d\u592a\u6b63\u78ba\uff0c\u4f46\u610f\u7fa9\u7b97\u662f\u6709\u5230\u3002\u6240\u4ee5\u5982\u679c\u5728\u65e5\u5e38\u88e1\u807d\u5230\uff0c\u7b97\u662f\u540c\u7a2e\u6aa2\u6e2c\uff0c\u53ef\u4ee5\u5c07\u5c31\u4e00\u4e0b\u3002\u70ba\u4ec0\u9ebc\u8aaa RT-qPCR \u6aa2\u6e2c\u53ef\u4ee5\u5c07\u73fe\u884c\u72af SARS-CoV-2 \u5728\u72af\u7f6a\u73fe\u5834\u902e\u500b\u6b63\u8457\u5462\uff1f<\/p>\n

SARS-CoV-2 \u8aaa\u5230\u5e95\uff0c\u7562\u7adf\u662f\u500b\u75c5\u6bd2\u3002SARS-CoV-2 \u672c\u8eab\u96b8\u5c6c\u65bc\u51a0\u72c0\u75c5\u6bd2\u79d1\uff08Coronavirinae\uff09\u3002\u75c5\u6bd2\u540d\u88e1\u982d\u7684 \u300cCoV\u300d\uff0c\u5c31\u662f\u51a0\u72c0\u75c5\u6bd2\u7684\u610f\u601d\u3002SARS-CoV-2 \u96d6\u7136\u9019\u6b21\u6bba\u5f97\u4eba\u985e\u63aa\u624b\u4e0d\u53ca\uff0c\u6b77\u53f2\u4e0a\uff0c\u6211\u5011\u548c\u51a0\u72c0\u75c5\u6bd2\u7684\u4ea4\u624b\u537b\u7d55\u975e\u53f2\u7121\u524d\u4f8b\u3002\u6709\u4e9b\u6703\u9020\u6210\u5c0b\u5e38\u611f\u5192\u75c7\u72c0\u7684\u75c5\u6bd2\uff0c\u5c31\u540c\u6a23\u4e5f\u662f\u96b8\u5c6c\u65bc\u51a0\u72c0\u75c5\u6bd2\u79d1\uff0c\u4f46\u5728\u611f\u67d3\u4eba\u985e\u4e4b\u5f8c\uff0c\u6bba\u50b7\u529b\u6bd4\u8f03\u8f15\u5fae\uff0c\u7b97\u662f\u79d1\u88e1\u7684\u7121\u540d\u5c0f\u5352\u3002\u4f46\uff0c\u4eba\u5916\u6709\u4eba\uff0c\u51a0\u72c0\u75c5\u6bd2\u79d1\u88e1\u9762\uff0c\u4e5f\u6709\u6bba\u624b\u7d1a\u7684\u89d2\u8272\u3002\u50cf\u662f 2003 \u5e74\u5728\u53f0\u7063\u7206\u767c\u75ab\u60c5\u7684 SARS \u91cd\u6025\u6027\u547c\u5438\u9053\u75c7\u5019\u7fa4\u51a0\u72c0\u75c5\u6bd2\uff0c\u9084\u67092015 \u5e74\u5728\u97d3\u570b\u8086\u8650\u7684 MERS-CoV \u4e2d\u6771\u547c\u5438\u75c7\u5019\u7fa4\u51a0\u72c0\u75c5\u6bd2\uff0c\u5c31\u662f\u4ee4\u4eba\u9aee\u6307\u7684\u89d2\u8272\u3002<\/p>\n

\u65e2\u7136 SARS-CoV-2 \u7562\u7adf\u662f\u500b\u75c5\u6bd2\uff0c\u5176\u8eab\u5bb6\u8d77\u5e95\uff0c\u4e5f\u81ea\u7136\u4e5f\u5c31\u6709\u8457\u75c5\u6bd2\u7684\u7279\u5fb5\u3002\u4e00\u5982\u51a0\u72c0\u75c5\u6bd2\u79d1\u7684\u5176\u4ed6\u75c5\u6bd2\uff0cSARS-CoV-2 \u5916\u982d\u5305\u8457\u5916\u5957\u819c\uff0c\u5713\u9f13\u9f13\u7684\uff0c\u5e36\u8457\u8a31\u591a\u7a81\u8d77\u3002\u90a3\u4e9b\u7a81\u8d77\u56e0\u70ba\u72c0\u4f3c\u7687\u51a0\uff0c\u800c\u8b93\u9019\u79d1\u75c5\u6bd2\u56e0\u6b64\u5f97\u6b64\u540d\u3002\u9019\u4e9b\u7687\u51a0\u72c0\u7a81\u8d77\u5c0d\u51a0\u72c0\u75c5\u6bd2\u4f86\u8aaa\uff0c\u662f\u611f\u67d3\u7d30\u80de\u7684\u6b66\u5668\uff0c\u63a5\u4e0b\u4f86\u4e5f\u6703\u597d\u597d\u4ecb\u7d39\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u51a0\u72c0\u75c5\u6bd2\u9084\u662f\u55ae\u80a1\u3001\u6b63\u93c8\u3001RNA\u75c5\u6bd2\u3002\u800c RT-qPCR \u6aa2\u6e2c\uff0c\u5c31\u662f\u5c07\u77db\u982d\u5c0d\u6e96SARS-CoV-2 \u6240\u7368\u6709\u7684 RNA \u5e8f\u5217\u3002SARS-CoV-2 \u6240\u5e36\u6709\u7684\u7368\u7279 RNA \u5e8f\u5217\uff0c\u5c31\u50cf\u5176\u7368\u5c6c\u7684\u75c5\u6bd2\u6307\u7d0b\u3002\u4f46\u662f\uff0c\u6bd4\u5728\u72af\u7f6a\u73fe\u5834\u641c\u627e\u7f6a\u72af\u6307\u7d0b\u8981\u6176\u5e78\u7684\u662f\uff0cRT-qPCR \u7684\u6aa2\u6e2c\u8981\u9748\u654f\u592a\u591a\u4e86\u3002<\/p>\n

\u4f46\u9019\u9748\u654f\u6027\uff0c\u537b\u4e5f\u662f RT-qPCR \u6aa2\u6e2c\u7684\u963f\u57fa\u91cc\u65af\u8171\u3002<\/p>\n

\u6211\u5011\u5148\u4f86\u5984\u60f3\u4e00\u4e0b\uff0c\u5982\u679c\u53ef\u4ee5\u8ddf\u963f\u62c9\u4e01\u795e\u71c8\u8a31\u9858\u4e00\u7a2e\u80fd\u5920\u8b93 SARS-CoV-2 \u7121\u6240\u9041\u5f62\u7684\u6aa2\u6e2c\uff0c\u4f86\u62ef\u6551\u597d\u53ef\u6190\u7684 2020 \u5e74\uff0c\u4f60\u6703\u5982\u4f55\u8ddf\u795e\u71c8\u8aaa\u5462\uff1f<\/p>\n

\u6211\u89ba\u5f97\uff0c\u4f86\u8ddf\u795e\u71c8\u6c42\u4e00\u7a2e\uff0c\u80fd\u5920\u65e2\u300c\u9748\u654f\u300d\u53c8\u300c\u6e96\u78ba\u300d\u5730\u7be9\u6aa2\u51fa\u5177\u6709\u628a SARS-CoV-2 \u300c\u50b3\u67d3\u7d66\u5176\u4ed6\u4eba\u5371\u96aa\u6027\u300d\u7684\u611f\u67d3\u8005\uff0c\u5982\u4f55\uff1f<\/p>\n

\u70ba\u4ec0\u9ebc\u9019\u4e09\u9805\u7279\u9ede\u5f88\u91cd\u8981\u5462\uff1f\u9996\u5148\uff0c\u7576\u524d\u75ab\u60c5\u5371\u96e3\u88e1\uff0c\u7be9\u6aa2\u6e2c\u9a57\u7684\u6700\u4e3b\u8981\u76ee\u6a19\uff0c\u662f\u80fd\u5920\u7be9\u51fa\u5177\u6709\u50b3\u67d3\u6027\u7684\u611f\u67d3\u8005\u3002\u6aa2\u6e2c\u8981\u65e2\u9748\u654f\u53c8\u6e96\u78ba\uff0c\u9019\u6bd4\u8f03\u597d\u7406\u89e3\u3002\u6211\u5011\u5e0c\u671b\u6aa2\u6e2c\u8b93\u75c5\u6bd2\u7121\u6240\u9041\u5f62\uff08\u9748\u654f\u5ea6\u9ad8\uff09\uff0c\u540c\u6642\uff0c\u6aa2\u6e2c\u7d50\u679c\u9084\u8981\u80fd\u5920\u88ab\u7d55\u5c0d\u4fe1\u8cf4\uff08\u5c08\u4e00\u5ea6\u9ad8\uff09\u3002<\/p>\n

\u4e0d\u904e\uff0c\u8d8a\u4f86\u8d8a\u591a\u7684\u81e8\u5e8a\u6578\u64da\u986f\u793a\uff0c\u4e0d\u662f\u6240\u6709\u4eba\u5728\u611f\u67d3 SARS-CoV-2 \u4e4b\u5f8c\u90fd\u6703\u51fa\u73fe\u8b93\u60a3\u8005\u300c\u610f\u8b58\u5230\u300d\u81ea\u5df1\u51fa\u73fe\u985e\u4f3c\u65bc\u611f\u5192\u7684\u75c7\u72c0\u3002\u53ef\u5606\u7684\u662f\uff0c\u547c\u5438\u9053\u75c7\u72c0\u7684\u56b4\u91cd\u7a0b\u5ea6\uff0c\u4f3c\u4e4e\u548c\u611f\u67d3\u8005\u7684\u50b3\u67d3\u529b\u5ea6\uff0c\u6c92\u6709\u76f4\u63a5\u95dc\u806f\u3002\u800c\u662f\u548c\u611f\u67d3\u7684\u6642\u9593\u8ef8\u6bd4\u8f03\u6709\u95dc\u3002<\/p>\n

\"\"<\/p>\n

\u9019\u662f\u4ec0\u9ebc\u610f\u601d\u5462\uff1f\u76ee\u524d\uff0c\u79d1\u5b78\u5bb6\u8207\u91ab\u7642\u754c\u7684\u5171\u8b58\u662f\uff0c\u50b3\u67d3\u529b\uff08transmissibility\uff09\u548c\u75c5\u6bd2\u91cf\uff08virus load\uff09\u6709\u8457\u7d55\u5c0d\u6b63\u6bd4\u95dc\u4fc2\u3002\u5e36\u539f\u7684\u75c5\u6bd2\u91cf\u6108\u591a\uff0c\u8a72\u500b\u9ad4\u7684\u50b3\u67d3\u529b\u5c31\u6703\u6108\u9a5a\u4eba\u3002\u800c\u75c5\u6bd2\u91cf\u591a\u5be1\uff0c\u5247\u548c\u88ab\u611f\u67d3\u7684\u6642\u9593\u8ef8\u6709\u660e\u986f\u76f8\u95dc\u3002<\/p>\n

\u75c5\u6bd2\u91cf\u5c0d\u611f\u67d3\u50b3\u64ad\u529b\u7684\u91cd\u8981\u6027\uff0c\u4e5f\u76f4\u63a5\u5f71\u97ff\u5230\u9632\u75ab\u5ba3\u5c0e\u76f8\u95dc\u7684\u653f\u7b56\u5ba3\u5c0e\u3002\u9019\u4e5f\u7684\u78ba\u662f\u70ba\u4ec0\u9ebc\u8981\u8033\u63d0\u9762\u547d\u5730\u4e00\u518d\u63d0\u9192\u5927\u5bb6\uff0c\u7d55\u5c0d\u8981\u52e4\u6d17\u624b\u3001\u4e0d\u6478\u81c9\u3001\u4e56\u4e56\u6234\u53e3\u7f69\u3001\u52aa\u529b\u4fdd\u6301\u793e\u4ea4\u8ddd\u96e2\u3002\u56e0\u70ba\uff0c\u63a5\u89f8\u7684\u75c5\u6bd2\u91cf\u6108\u5c11\uff0c\u88ab\u611f\u67d3\u7684\u6a5f\u6703\u5c31\u6108\u5c11\uff1b\u63a5\u89f8\u7684\u75c5\u6bd2\u91cf\u6108\u5c11\uff0c\u611f\u67d3\u75c7\u72c0\u4e5f\u6703\u6bd4\u8f03\u8f15\u5fae\u5594\u3002\u4f60\u8aaa\u9019\u662f\u4e0d\u662f\u4e00\u77f3\u597d\u5e7e\u9ce5\uff0c\u4fdd\u8b77\u81ea\u5df1\u4e5f\u4fdd\u8b77\u773e\u4eba\u5462\u3002<\/p>\n

\u4f46\uff0c\u9019\u4e5f\u8b93\u6aa2\u6e2c\u7d50\u679c\u9019\u975e\u9ed1\u5373\u767d\u7684\u662f\u975e\u984c\uff0c\u7121\u6cd5\u63d0\u4f9b\u4e00\u500b\u5b8c\u7f8e\u5730\u3001\u80fd\u5920\u4e00\u7ffb\u5169\u77aa\u773c\u7684\u7d55\u5c0d\u6b63\u78ba\u7b54\u6848\u3002<\/p>\n

\u5728\u500b\u9ad4\u88ab\u611f\u67d3\u4e4b\u5f8c\uff0c\u76ee\u524d\u89ba\u5f97\u75c7\u72c0\u51fa\u73fe\u524d\u5c31\u6709\u611f\u67d3\u529b\uff0c\u4f46\u6700\u8b93\u4eba\u63ea\u5fc3\u76ba\u7709\u982d\u7684\uff0c\u9084\u662f\u5728\u75c7\u72c0\u51fa\u73fe\u7684\u4e94\u5230\u516b\u5929\u5167\u3002\u5404\u5730\u65b9\u7684\u6578\u64da\u96d6\u7136\u591a\u5c11\u6709\u9ede\u5dee\u7570\uff0c\u4f46\u5927\u62b5\u812b\u4e0d\u51fa\u9019\u7bc4\u570d\u3002\u6211\u5011\u53ef\u4ee5\u4f86\u77a7\u77a7\u53f0\u7063\u81ea\u5df1\u51fa\u54c1\u7684\u4e00\u7bc7\u6f02\u4eae\u7814\u7a76\u3002\u7531\u6b66\u6f22\u80ba\u708e\u75ab\u60c5\u7814\u7a76\u5c0f\u7d44\uff08Taiwan COVID-19 outbreak investigation team\uff09\u8207\u53f0\u7063\u5927\u5b78\u516c\u5171\u885b\u751f\u5b78\u9662\u6797\u5148\u548c\u6559\u6388\u5be6\u9a57\u5ba4\u3001\u8207\u75be\u75c5\u7ba1\u5236\u7f72\u651c\u624b\u5408\u4f5c\uff0c\u5728 Jama Internal Medicine <\/em>\u4e0a\u767c\u8868\u4e86\u4e00\u7bc7\u4ee5\u53f0\u7063\u75ab\u60c5\u6578\u64da\u70ba\u57fa\u790e\uff0c\u5c0d\u50b3\u67d3\u529b\u7684\u5206\u6790\u7814\u7a76\u3002<\/p>\n

\u9019\u7bc7\u7814\u7a76\u88e1\uff0c\u7d71\u8a08\u8ffd\u8e64\u4e86\u4e00\u6708\u4e2d\u5230\u4e09\u6708\u4e2d\uff0c\u63a5\u89f8\u5230\u78ba\u8a3a\u500b\u6848\u800c\u9700\u5c45\u5bb6\u9694\u96e2\u5341\u56db\u5929\u7684\u4eba\u3002\u5728\u4e00\u767e\u4f4d\u53c3\u8207\u7814\u7a76\u7684\u78ba\u8a3a\u75c5\u4eba\u88e1\uff0c\u5171\u8ffd\u8e64\u4e86\u5169\u5343\u4e03\u767e\u591a\u4f4d\u548c\u78ba\u8a3a\u75c5\u60a3\u6709\u5bc6\u5207\u63a5\u89f8\u8005\u3002\u5176\u4e2d\uff0c \u56e0\u70ba\u5bc6\u5207\u63a5\u89f8\u800c\u88ab\u611f\u67d3\u7684\u6bd4\u4f8b\u7d04\u70ba 0.7 \uff05\uff0c\u4f46\u5982\u679c\u4ed4\u7d30\u5206\u6790\u9019\u4e9b\u4eba\u8207\u78ba\u8a3a\u75c5\u60a3\u7684\u63a5\u89f8\u53f2\uff0c\u6703\u767c\u73fe\u78ba\u8a3a\u75c5\u60a3\u7684\u611f\u67d3\u6642\u9593\u8ef8\uff0c\u5c0d\u50b3\u64ad\u529b\u6709\u8457\u975e\u5e38\u95dc\u9375\u7684\u89d2\u8272\u3002\u6700\u5371\u96aa\u7684\u6642\u5019\uff0c\u5927\u6982\u662f\u78ba\u8a3a\u60a3\u8005\u51fa\u73fe\u81e8\u5e8a\u75c7\u72c0\u7684\u4e94\u5929\u4ee5\u5167\uff0c\u9084\u6709\u75c7\u72c0\u51fa\u73fe\u4e4b\u524d\uff1b\u5728\u8f03\u665a\u7684\u6642\u9593\u9ede\u63a5\u89f8\u5230\u78ba\u8a3a\u75c5\u60a3\uff0c\u50b3\u67d3\u529b\u5c31\u76f8\u5c0d\u5c0f\u5f88\u591a\u5f88\u591a\u3002<\/p>\n

\u56de\u5230 RT-qPCR \u6aa2\u6e2c\u3002\u76ee\u524d\u7684\u6aa2\u6e2c\u7684\u78ba\u8b93\u4eba\u81ea\u8c6a\uff1b\u4e0d\u50c5\u7be9\u9a57\u6642\u7a0b\u6240\u9700\u6642\u9593\u6108\u4f86\u6108\u77ed\uff0c\u800c\u4e14\u8d85\u7d1a\u9748\u654f\u3002\u4f46\u70ba\u4ec0\u9ebc\u9019\u8d85\u7d1a\u5f37\u608d\u7684\u9748\u654f\u5ea6\uff0c\u6703\u662f\u500b\u554f\u984c\u5462\uff1f<\/p>\n

\u56e0\u70ba\uff0c\u5728\u81e8\u5e8a\u75c7\u72c0\u51fa\u73fe\u4e4b\u5f8c\u7684\u4e94\u500b\u79ae\u62dc\u5167\uff0c\u90fd\u88ab\u767c\u73fe\u5f88\u6709\u53ef\u80fd\u6703\u51fa\u73fe\u6aa2\u9a57\u967d\u6027\u7684\u7d50\u679c\u554a\u3002\u5f88\u591a\u6642\u5019\uff0c\u60a3\u8005\u7684\u81e8\u5e8a\u75c7\u72c0\u4e5f\u90fd\u65e9\u5df2\u6d88\u5f4c\u75ca\u7652\u3002\u800c\u4e14\u5927\u90e8\u5206\u5c0d\u75c5\u60a3\u7684\u8ffd\u8e64\u7be9\u6aa2\uff0c\u767c\u73fe\u5f9e\u75c7\u72c0\u51fa\u73fe\uff0c\u5230\u6aa2\u9a57\u7d50\u679c\u9670\u6027\u7684\u6240\u9700\u6642\u9593\uff0c\u4e2d\u4f4d\u6578\u5927\u81f4\u4ecb\u65bc 24 \u5929\u5de6\u53f3\u3002\u5c0d\u65bc\u5e74\u7d00\u6bd4\u8f03\u5927\u3001\u6216\u662f\u6709\u7cd6\u5c3f\u75c5\u3001\u9ad8\u8840\u58d3\u75c5\u53f2\u7684\u75c5\u60a3\uff0c\u4e5f\u88ab\u767c\u73fe\u9700\u8981\u66f4\u591a\u7684\u6642\u9593\uff0c\u624d\u80fd\u5920\u5f97\u5230\u9670\u6027\u6aa2\u9a57\u7d50\u679c\u3002\u751a\u81f3\uff0c\u5c62\u5c62\u4e5f\u6709\u5831\u5c0e\uff0c\u767c\u73fe\u5c31\u7b97\u9023\u7e8c\u5169\u6b21\u6aa2\u9a57\u7d50\u679c\u9670\u6027\uff0c\u4e4b\u5f8c\u9084\u662f\u5f88\u6709\u53ef\u80fd\u51fa\u73fe\u967d\u6027\u3002<\/p>\n

\u9019\u9ebb\u7169\u8655\u6b63\u5728\u65bc\uff0c\u6aa2\u9a57\u7d50\u679c\u5617\u8a66\u7528\u300c\u662f\u300d\u3001\u8207\u300c\u4e0d\u662f\u300d\u4f86\u63d0\u4f9b\u6aa2\u75ab\u4e0a\u7684\u5224\u65b7\u57fa\u6e96\uff0c\u4f46\u5f80\u5f80\u6c92\u6709\u63d0\u4f9b\u75c5\u60a3\u75c5\u6bd2\u91cf\u7684\u8cc7\u8a0a\u3002<\/p>\n

\u6709\u8da3\u7684\u662f\uff0c\u5176\u5be6\u75c5\u6bd2\u91cf\u7684\u8cc7\u8a0a\uff0c\u6b63\u6084\u6084\u57cb\u85cf\u5728 RT-qPCR \u6aa2\u6e2c\u88e1\u3002<\/p>\n

RT-qPCR \u6aa2\u6e2c\u600e\u9ebc\u505a\u5462\uff1f\u8b93\u6211\u5011\u4f86\u5047\u8a2d\uff0c\u6aa2\u9a57\u4e2d\u5fc3\u6536\u5230\u4e86\u4e00\u500b\u88ab\u611f\u67d3\u60a3\u8005\u7684\u6aa2\u9ad4\u3002\u9996\u5148\uff0cSARS-CoV-2 \u75c5\u6bd2\u7684 RNA \u6703\u5148\u88ab\u53cd\u8f49\u9304\u3001\u751f\u6210 DNA \uff08RT \u6b65\u9a5f\uff09\uff0c\u7136\u5f8c\uff0c\u5728 qPCR \u9019\u4e00\u6b65\u5be6\u9a57\u6d41\u7a0b\u88e1\uff0c\u88ab\u5075\u6e2c\u7684\u6838\u9178\u5e8f\u5217\u8a0a\u865f\u6703\u88ab\u4e00\u6b65\u53c8\u4e00\u6b65\uff0c\u96a8\u8457 PCR \u7684\u5be6\u9a57\u6d41\u7a0b\u800c\u88ab\u9010\u6f38\u653e\u5927\uff0c\u904e\u7a0b\u88e1\u7684\u87a2\u5149\u8a0a\u865f\uff0c\u53ef\u88ab\u6211\u5011\u8b80\u53d6\u3002\u795e\u5947\u5730\u662f\uff0c\u87a2\u5149\u8a0a\u865f\u6703\u548c\u300c\u9700\u8981\u300d\u88ab\u5075\u6e2c\u7684\u6838\u9178\u91cf\u6210\u6b63\u6bd4\uff0c\u9019\u4e5f\u662f\u70ba\u4ec0\u9ebc\u80fd\u5920\u63d0\u4f9b\u300c\u5b9a\u91cf\u300d\u7684\u8a0a\u606f\uff08\u4e5f\u5c31\u662f\u90a3\u500b\u300eq\u300f\uff09\u3002\u63db\u53e5\u8a71\u8aaa\uff0c\u5982\u679c\u6aa2\u9ad4\u88e1\u4e00\u525b\u958b\u59cb\u7684\u75c5\u6bd2 RNA \u91cf\u591a\uff0c\u5728\u4e00\u6b65\u53c8\u4e00\u6b65\u7684\u653e\u5927\u6d41\u7a0b\u88e1\uff0c\u87a2\u5149\u8a0a\u865f\u5c31\u6703\u88ab\u6025\u5287\u653e\u5927\u3002\u8ddf\u75c5\u6bd2\u91cf\u5c11\u7684\u6aa2\u9ad4\u6bd4\u8f03\u8d77\u4f86\uff0c\u75c5\u6bd2\u91cf\u300c\u591a\u300d\u7684\u6aa2\u9ad4\u9700\u8981\u6bd4\u8f03\u300c\u5c11\u300d\u7684\u653e\u5927\u6b65\u9a5f\u6578\uff0c\u5c31\u53ef\u4ee5\u5c07\u6211\u5011\u60f3\u8981\u5075\u6e2c\u7684\u6838\u9178\u5e8f\u5217\u8a0a\u865f\uff08\u4e5f\u5c31\u662f\u8b80\u53d6\u7684\u87a2\u5149\u8a0a\u865f\uff09\u653e\u5927\u5230\u4e00\u5b9a\u7a0b\u5ea6\u3002\u9019\u500b\u653e\u5927\u6b65\u9a5f\u6578\u4ee5\u6bd4\u8f03\u79d1\u5b78\u7684\u5c08\u696d\u8853\u8a9e\u4f86\u63cf\u8ff0\uff0c\u5c31\u662f Ct \u503c\u3002\u800cCt \u503c\u6240\u6307\u5f97\uff0c\u662f\u8de8\u904e\u95be\u503c\u6240\u9700\u8981\u7684\u5faa\u74b0\u6b65\u9a5f\u6578\u76ee\uff08threshold cycle value\uff09\u3002Ct \u503c\u6bcf\u589e\u52a0 3.3\uff0c\u6700\u521d\u6aa2\u9ad4\u88e1\u7684\u75c5\u6bd2\u91cf\uff0c\u5c31\u5c11\u5341\u500d\u3002\u800c Ct \u503c\u6108\u4f4e\uff0c\u75c5\u6bd2\u91cf\u5c31\u6108\u591a\u3002<\/p>\n

\u4f60\u767c\u73fe\u5176\u4e2d\u7684\u95dc\u9375\u4e86\u55ce\uff1f\u91cd\u9ede\u4e0d\u55ae\u662f\u9670\u6027\u9084\u662f\u967d\u6027\uff0c\u9084\u6709\uff0c\u9700\u8981\u591a\u5c11\u5faa\u74b0\u6b65\u9a5f\uff0c\u80fd\u5920\u6709\u6548\u5730\u5c07\u75c5\u6bd2\u6838\u9178\u5e8f\u5217\u653e\u5927\u5230\u4e00\u5b9a\u7a0b\u5ea6\u3002\u901a\u5e38\u5728\u4e00\u5b9a Ct \u503c\u5167\uff0c\u6703\u88ab\u5224\u5b9a\u967d\u6027\u53cd\u61c9\u3002\u53f0\u7063\u76ee\u524d\u6240\u63a1\u53d6\u7684\u754c\u7dda\u662f Ct \u503c\u9700\u4f4e\u65bc 35\u3002<\/p>\n

\u5728\u76ee\u524d\u8ffd\u8e64\u78ba\u8a3a\u75c5\u60a3\u7684\u6587\u737b\u5831\u544a\uff0c\u5e38\u5e38\u767c\u73fe\u5728\u5f8c\u6bb5\u7684\u5e7e\u500b\u79ae\u62dc\u6642\u9593\u88e1\uff0cCt \u503c\u90fd\u504f\u9ad8\uff0c\u751a\u81f3\u4ecb\u65bc\u8a72\u570b\u5224\u65b7\u9670\u6027\u9084\u662f\u967d\u6027\u7684\u908a\u754c\u4e0a\uff0c\u800c\u9019\u4e5f\u5f80\u5f80\u4ee3\u8868\u6aa2\u9ad4\u88e1\u7684\u75c5\u6bd2\u91cf\u5df2\u7d93\u5f88\u4f4e\u3002<\/p>\n

\"\"<\/p>\n

\u63db\u8a00\u4e4b\uff0cRT-qPCR \u6aa2\u6e2c\u975e\u5e38\u91cd\u8981\uff0c\u5c24\u5176\u5728\u65e9\u671f\u500b\u6848\u8ffd\u8e64\u88e1\uff0c\u80fd\u5920\u5354\u52a9\u9632\u75ab\u4e0a\u7684\u5224\u65b7\u3002\u4f46\u4e5f\u9700\u8981\u8a18\u4f4f\u7684\u662f\uff0cRT-qPCR \u6aa2\u6e2c\u7d50\u679c\uff0c\u548c\u500b\u9ad4\u662f\u4e0d\u662f\u4ecd\u7136\u5177\u6709\u611f\u67d3\u529b\uff0c\u537b\u4e0d\u4e00\u5b9a\u80fd\u5920\u76f4\u63a5\u5283\u4e0a\u7b49\u865f\u3002\u9019\u662f\u56e0\u70ba RT-qPCR \u6aa2\u6e2c\u5be6\u5728\u662f\u592a\u654f\u611f\u4e86\uff0c\u662f\u6293 SARS-CoV-2 \u73fe\u884c\u72af\u7684\u7d55\u4f73\u5075\u63a2\u3002\u4f46\u9019\u5075\u63a2\uff0c\u6709\u500b\u72d7\u9f3b\u5b50\uff0c\u96d6\u7136\u80fd\u5920\u55c5\u51fa SARS-CoV-2\uff0c\u4f46\u56e0\u70ba\u5be6\u5728\u662f\u592a\u654f\u611f\u4e86\uff0c\u5982\u679c\u9019\u73fe\u884c\u72af\u5df2\u7d93\u6b7b\u4e86\u3001\u6216\u662f\u55aa\u5931\u884c\u52d5\u80fd\u529b\u4e86\uff0c\u9084\u662f\u96e3\u9003\u90a3\u52d5\u4e00\u52d5\u5c31\u80fd\u5920\u55c5\u51fa\u4e0d\u5c0d\u52c1\u7684\u72d7\u9f3b\u5b50\u3002<\/p>\n

\u800c\u76f8\u8f03\u65bc RT-qPCR\uff0c\u8840\u6e05\u5b78\u6aa2\u6e2c\u5247\u662f\u628a\u77db\u982d\u6307\u5411\u500b\u9ad4\u7684\u75c5\u6bd2\u611f\u67d3\u53f2\u3002\u8840\u6e05\u5b78\u6aa2\u6e2c\u7684\u5047\u8a2d\u5f88\u7c21\u55ae\uff0c\u6240\u8b02\u51e1\u8d70\u904e\u5fc5\u7559\u4e0b\u75d5\u8de1\u3002\u800c\u75c5\u6bd2\u611f\u67d3\uff0c\u4e5f\u662f\u4e00\u6a23\u3002\u53ea\u8981\u514d\u75ab\u7cfb\u7d71\u6c92\u6709\u75b2\u5f31\u800c\u5c0d\u75c5\u6bd2\u8996\u800c\u4e0d\u898b\uff0c\u514d\u75ab\u7cfb\u7d71\u4e0d\u6703\u5750\u4e4b\u4e0d\u7406\u3002\u514d\u75ab\u7cfb\u7d71\u4e00\u65e6\u555f\u52d5\uff0c\u5148\u5929\u514d\u75ab\u8207\u5f8c\u5929\u514d\u75ab\u7cfb\u7d71\u90fd\u6703\u52a0\u5165\u5c0d\u6297\u75c5\u6bd2\u7684\u4f5c\u6230\uff0c\u4e26\u4e14\u9075\u7167\u4e00\u5b9a\u7684\u555f\u52d5\u6d41\u7a0b\u3002\u800c\u8840\u6e05\u5b78\u5c31\u662f\u6839\u64da\u6211\u5011\u5c0d\u514d\u75ab\u7cfb\u7d71\u555f\u52d5\u6d41\u7a0b\u7684\u7406\u89e3\uff0c\u4f86\u76e3\u770b\u5c0d SARS-CoV-2 \u7684\u514d\u75ab\u529b\uff0c\u4f86\u5224\u65b7\u500b\u9ad4\u662f\u4e0d\u662f\u66fe\u7d93\u88ab\u611f\u67d3\u3002\u5f8c\u5929\u514d\u75ab\u7cfb\u7d71\u6240\u9700\u53cd\u61c9\u6642\u9593\u8f03\u9577\uff0c\u5927\u7d04\u8981\u4e94\u5230\u4e03\u5929\u624d\u80fd\u5920\u88ab\u555f\u52d5\uff0c\u4f46\u80fd\u5920\u63d0\u4f9b\u514d\u75ab\u529b\u3002\u5728\u9019\u6bb5\u6642\u9593\u88e1\uff0c\u500b\u9ad4\u80fd\u5920\u7522\u751f\u6297\u9ad4 IgG \u6240\u9700\u7684\u6642\u9593\uff0c\u53c8\u6bd4 IgM \u8981\u9577\u3002\u56e0\u6b64\uff0c\u7be9\u6aa2\u662f\u4e0d\u662f\u5177\u6709\u5c0d SARS-CoV-2 \u6709\u5c08\u4e00\u6027\u7684 IgG \u6297\u9ad4\uff0c\u53ef\u4ee5\u5927\u6982\u63a8\u6e2c\u51fa\u500b\u9ad4\u662f\u4e0d\u662f\u66fe\u7d93\u611f\u67d3\u904e\u3002<\/p>\n

\u7e3d\u800c\u8a00\u4e4b\uff0cqRT-PCR \u6aa2\u9a57\u4e5f\u597d\uff0c\u8840\u6e05\u5b78\u6aa2\u9a57\u4e5f\u597d\uff0c\u662f\u5404\u6709\u5343\u79cb\u8207\u64c5\u5834\uff0c\u80fd\u5920\u63d0\u4f9b\u7684\u611f\u67d3\u75c5\u8cc7\u8a0a\u4e5f\u6709\u5dee\u7570\u3002\u7aef\u8cf4\u7684\u662f\uff0c\u662f\u4e0d\u662f\u80fd\u5920\u5c0d\u6aa2\u9a57\u7d50\u679c\uff0c\u597d\u597d\u5730\u89e3\u8b80\u5594\u3002<\/p>\n

 <\/p>\n

\u53c3\u8003\u8cc7\u6599<\/strong><\/p>\n

    \n
  1. Hao-Yuan Cheng, Shu-Wan Jian, Ding-Ping Liu, Ta-Chou Ng, Wan-Ting Huang, Hsien-Ho Lin, Taiwan COVID-19 Outbreak Investigation Team. Contact Tracing Assessment of COVID-19 Transmission Dynamics in Taiwan and Risk at Different Exposure Periods Before and After Symptom Onset. JAMA Intern Med.<\/em> 2020 May 1;e202020. doi: 10.1001\/jamainternmed.2020.2020. Online ahead of print.<\/li>\n
  2. Xi He, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med<\/em>. 2020 May;26(5):672-675. doi: 10.1038\/s41591-020-0869-5. Epub 2020 Apr 15.<\/li>\n
  3. Michael R Tom and Michael J Mina. To Interpret the SARS-CoV-2 Test, Consider the Cycle Threshold Value. Clin Infect Dis. 2020 May 21 : ciaa619.<\/li>\n
  4. Antonio La Marca, et al. Testing for SARS-CoV-2 (COVID-19): a systematic review and clinical guide to molecular and serological in-vitro diagnostic assays. Reprod Biomed Online. 2020 Jun 14;S1472-6483(20)30318-7. doi: 10.1016\/j.rbmo.2020.06.001. Online ahead of print.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u8f49\u773c\u9593\uff0c2020 \u5e74\u590f\u5929\u5c31\u9019\u6a23\u4f86\u4e86\u3002\u5927\u534a\u5e74\u88e1\uff0c\u300c\u56b4\u91cd\u7279\u6b8a\u50b3\u67d3\u6027\u80ba\u708e\u300dCOVID-19 \uff08\u4ea6\u7a31\u6b66\u6f22\u80ba\u708e\uff09\u7684\u75ab\u60c5\u5e2d\u6372\u5168\u7403\uff0c\u5982\u4eca\u4f9d\u7136\u5f35\u72c2\u3002\u800c\u53f0\u7063\uff0c\u50cf\u500b\u4e16\u5916\u6843\u6e90\u3002\u63a5\u4e0b\u4f86\uff0c\u5728\u9019\u4e00\u7cfb\u5217\u5c08\u984c\u88e1\uff0c\u6211\u5011\u5c31\u4e00\u8d77\u4f86\u8a0e\u8ad6\u9019\u5927\u534a\u5e74\u88e1\uff0c\u5728\u81e8\u5e8a\u8207\u7814\u7a76\u524d\u7dda\u4e0a\uff0c\u6211\u5011\u5c0d\u65bc\u9019\u60e1\u540d\u662d\u5f70\u7684 COVID-19 \u8207\u7f6a\u9b41\u798d\u9996 SARS-CoV-2\uff0c\u5404\u754c\u5c08\u5bb6\u7aae\u76e1\u5fc3\u529b\u6240\u6162\u6162\u641e\u6e05\u695a\uff08\u6216\u662f\u9084\u6c92\u5f04\u660e\u767d\uff09\u5730\u9019\u4e9b\u90a3\u4e9b\u4e8b\u5427\u3002\u8b93\u6211\u5011\u4e00\u8d77\u4f86\u4ee5\u75ab\u60c5\u70ba\u57fa\uff0c\u597d\u597d\u5730\u8a0e\u8ad6\u514d\u75ab\u5b78\u5427\uff01<\/p>\n","protected":false},"author":20,"featured_media":35558,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4580,3293],"tags":[4577,4522,4700,4523,4704],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2020\/08\/Cover-Fig.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/35557"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=35557"}],"version-history":[{"count":2,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/35557\/revisions"}],"predecessor-version":[{"id":35562,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/35557\/revisions\/35562"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/35558"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=35557"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=35557"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=35557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}