{"id":33362,"date":"2018-12-13T00:22:28","date_gmt":"2018-12-12T16:22:28","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=33362"},"modified":"2018-12-13T08:59:15","modified_gmt":"2018-12-13T00:59:15","slug":"%e3%80%90crispr-baby-%e7%b3%bb%e5%88%97%e4%b9%8b%e4%ba%8c%e3%80%91%e7%82%ba%e4%bb%80%e9%ba%bcccr5-%e5%9f%ba%e5%9b%a0%e6%98%af%e8%b3%80%e5%bb%ba%e5%a5%8e%e7%9a%84%e7%9c%bc%e8%a3%a1%e8%a5%bf%e6%96%bd","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=33362","title":{"rendered":"\u3010CRISPR baby \u7cfb\u5217\u4e4b\u4e8c\u3011\u70ba\u4ec0\u9ebcCCR5 \u57fa\u56e0\u662f\u8cc0\u5efa\u594e\u773c\u88e1\u7684\u897f\u65bd\uff1f"},"content":{"rendered":"

\u25a0\u7b2c\u4e00\u5c0d\u57fa\u56e0\u7de8\u8f2f\u5bf6\u5bf6\uff0c\u5c31\u9019\u6a23\u5728\u773e\u4eba\u7684\u9a5a\u6115\u4e2d\u8a95\u751f\u4e86\u3002\u4e2d\u570b\u5357\u65b9\u79d1\u6280\u5927\u5b78\u526f\u6559\u6388\u8cc0\u5efa\u594e\uff0c\u5229\u7528 CRISPR\/Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u5c07\u9019\u5c0d\u96d9\u80de\u80ce\u9ad4\u5167\u7684 CCR5 \u57fa\u56e0\u5254\u9664\uff0c\u5e0c\u671b\u9019\u5c0d\u96d9\u80de\u80ce\u7d42\u5176\u4e00\u751f\u90fd\u4e0d\u7528\u64d4\u5fc3\u88ab HIV \u75c5\u6bd2\u611f\u67d3\u3002\u8cc0\u5efa\u594e\u7684\u7406\u7531\uff0c\u5728\u79d1\u5b78\u502b\u7406\u524d\u88ab\u75db\u6279\u7684\u9ad4\u7121\u5b8c\u819a\uff1b\u90a3\u5728\u79d1\u5b78\u5b78\u7406\u9762\u524d\u5462\uff1f\u771f\u5982\u4ed6\u6240\u8a00\u4e00\u822c\uff0c\u7ad9\u5f97\u4f4f\u8173\u55ce\uff1f<\/strong><\/span><\/p>\n

\"\"\u64b0\u6587\uff5c\u99f1\u5b9b\u7433<\/strong><\/p>\n

\u8cc0\u5efa\u594e\u70ba\u4ec0\u9ebc\u770b\u4e0a CCR5\uff1fCCR5 \u7121\u7591\u662f\u5c0d\u6297 HIV \u75c5\u6bd2\u611f\u67d3\u8207\u6cbb\u7642\u5f8c\u5929\u514d\u75ab\u7f3a\u4e4f\u75c7\u5019\u7fa4\u7684\u5929\u738b\u7d1a\u89d2\u8272\u3002<\/p>\n

\u65e9\u5728 1983 \u5e74\u79d1\u5b78\u5bb6\u7be4\u5b9a HIV \u662f\u9020\u6210\u5f8c\u5929\u514d\u75ab\u7f3a\u4e4f\u75c7\u5019\u7fa4\u7684\u7f6a\u9b41\u798d\u9996\u4e4b\u524d\uff0c\u5c31\u6709\u8a31\u591a\u7814\u7a76\u4eba\u54e1\u767c\u73fe HIV \u7279\u5225\u504f\u611b\u514d\u75ab\u7cfb\u7d71\u88e1\u7684 CD4 T \u7d30\u80de\u3002HIV \u75c5\u6bd2\u85c9\u7531\u672c\u8eab\u8868\u9762\u7684 gp120 \u8207 gp41 \u5169\u7a2e\u91a3\u86cb\u767d\u800c\u5f97\u4ee5\u5165\u4fb5 T \u7d30\u80de\u3002HIV \u75c5\u6bd2\u5148\u9760 gp120 \u91a3\u86cb\u767d\u548c T \u7d30\u80de\u7684 CD4 \u63a5\u89f8\uff0c\u5169\u76f8\u7d50\u5408\u4e4b\u5f8c\uff0c\u6703\u9020\u6210 gp120 \u69cb\u578b\u4e0a\u7684\u6539\u8b8a\uff0c\u4f7f\u5176\u4e0a\u982d\u7684 V3 \u53ef\u8b8a\u74b0\u5340\uff08variable 3 loop\uff09\u80fd\u548c\u53e6\u4e00\u500b T \u7d30\u80de\u8868\u9762\u86cb\u767d\u8cea\uff08CCR5 \u6216\u662f CXCR4\uff09\u7d50\u5408\uff0c\u9032\u800c\u4f7f\u5f97 HIV \u8868\u9762\u7684 gp41 \u91a3\u86cb\u767d\u5f97\u4ee5\u63a5\u89f8\u5230 T \u7d30\u80de\u7d30\u80de\u819c\uff0c\u5728\u5176\u4e0a\u6253\u6d1e\uff0c\u597d\u8b93 HIV \u628a\u81ea\u5df1\u7684\u907a\u50b3\u7269\u8cea\u611f\u67d3\u5230 T \u7d30\u80de\u5167\u3002\u56e0\u6b64\uff0cHIV \u75c5\u6bd2\u8981\u611f\u67d3 T \u7d30\u80de\uff0c\u5fc5\u9808\u6e4a\u8db3 T \u7d30\u80de\u6709\u8868\u9054 CD4\u3001\u8207 CCR5\uff08\u6216\u662f CXCR4\uff09\u7684\u689d\u4ef6\u3002<\/p>\n

\u66f4\u8b93\u79d1\u5b78\u5bb6\u5fc3\u52d5\u7684\u662f\uff0c1996 \u5e74\u56db\u6708\u6642\uff0c\u7f8e\u570b\u7d10\u7d04 The Rockefeller University \u7684 Richard Koup \u6559\u6388\u5728Nature Medicine<\/em>\u671f\u520a\u4e0a\u767c\u8868\u4e00\u7bc7\u8b93\u4eba\u773c\u775b\u767c\u4eae\u7684\u8ad6\u6587\u3002<\/p>\n

Koup \u6559\u6388\u5be6\u9a57\u5ba4\u62db\u52df\u4e00\u7fa4\u66fe\u7d93\u591a\u6b21\u66b4\u9732\u65bc HIV \u75c5\u6bd2\u611f\u67d3\u98a8\u96aa\u4e2d\u3001\u537b\u5f9e\u672a\u88ab\u611f\u67d3\u7684\u6b50\u6d32\u4eba\uff0c\u767c\u73fe\u5176\u4e2d\u6709\u5169\u500b\u6b50\u6d32\u4eba\u7684 CD4 T \u7d30\u80de\u50cf\u662f\u7a7f\u4e86\u9632\u8b77\u7f69\u4e00\u6a23\uff0c\u5c0d HIV \u75c5\u6bd2\u7684\u62b5\u6297\u529b\u8981\u6bd4\u4e00\u822c\u4eba\u5f37\u5f88\u591a\u3002\u4f46\u9019\u9632\u8b77\u7f69\u4e5f\u4e0d\u662f\u9285\u7246\u9435\u58c1\uff1a\u7576 HIV \u75c5\u6bd2\u91cf\u9ad8\u5230\u4e00\u5b9a\u7a0b\u5ea6\u7684\u6642\u5019\u3001\u6216\u662f HIV \u75c5\u6bd2 V3 \u53ef\u8b8a\u74b0\u5340\u767c\u751f\u7a81\u8b8a\u7684\u6642\u5019\uff0c\u9019\u5169\u4f4d\u6b50\u6d32\u4eba\u7684 CD4 T \u7d30\u80de\u9632\u8b77\u7f69\u5c31\u6703\u5931\u53bb\u529f\u80fd\u3002\u800c\u4e14\uff0c\u9019\u5169\u4f4d\u6b50\u6d32\u4eba\u7684\u9632\u8b77\u7f69\uff0c\u53ea\u5c0d\u9700\u8981\u9760 CCR5 \u611f\u67d3 T \u7d30\u80de\u7684 HIV \u75c5\u6bd2\u7a2e\u6709\u6548\uff08\u7a31\u70ba R5 \u75c5\u6bd2\uff09\uff1b\u5c0d\u65bc\u53e6\u5916\u4e00\u7a2e\u9700\u8981\u9760 CXCR4 \u611f\u67d3 T \u7d30\u80de\u7684 HIV \u75c5\u6bd2\uff08\u7a31\u70ba X4 \u75c5\u6bd2\uff09\u3001\u6216\u662f\u65e2\u53ef\u900f\u904e CCR5 \u4e5f\u53ef\u4ee5\u9760 CXCR4 \u7684\u75c5\u6bd2\uff08X4R5\u75c5\u6bd2\uff09\u4ea6\u662f\u675f\u624b\u7121\u7b56\u3002<\/p>\n

\u540c\u5e74\u516b\u6708\uff0cKoup \u6559\u6388\u5be6\u9a57\u5ba4\u4e58\u52dd\u8ffd\u64ca\uff0c\u548c\u540c\u4e8bNathaniel Landau \u6559\u6388\u5be6\u9a57\u5ba4\u806f\u624b\uff0c\u5728 Cell <\/em>\u671f\u520a\u4e0a\u4e00\u8d77\u63ed\u958b\u4e86\u9019\u5169\u4f4d\u6b50\u6d32\u4eba\u7684\u9632\u8b77\u7f69\u5bc6\u6280\uff1a\u4ed6\u5011\u7684 CCR5<\/em> \u57fa\u56e0\u6709\u7a81\u8b8a\u904e\uff0c\u6bd4\u6b63\u5e38\u7684\u57fa\u56e0\u5e8f\u5217\u5c11\u4e86 32 \u500b\u9e7c\u57fa\u5c0d\uff0c\u4f7f\u5f97\u57fa\u56e0\u7a81\u8b8a\u8655\u4e4b\u5f8c\u7684\u57fa\u56e0\u7de8\u78bc\u7ae0\u6cd5\u5168\u4e82\uff0c\u7d42\u6b62\u5bc6\u78bc\u5b50\u63d0\u65e9\u51fa\u73fe\u3002\u56e0\u6b64\uff0cCCR5\u039432<\/em> \u6240\u7de8\u78bc\u7684\u86cb\u767d\u8cea\u5728\u8f49\u8b6f\u904e\u7a0b\u4e2d\u63d0\u65e9\u78b0\u5230\u7d42\u6b62\u5bc6\u78bc\u5b50\u800c\u5927\u7576\u6a5f\u3002\u800c\u88fd\u9020\u51fa\u4f86\u7684 CCR5\u039432 \u86cb\u767d\u8cea\uff0c\u4e0d\u4f46\u56e0\u70ba\u9019 32 \u500b\u9e7c\u57fa\u5c0d\u7684\u7f3a\u5931\uff0c\u800c\u6210\u4e86\u77ed\u5c0f\u664f\u5b30\u7248\uff0c\u9019\u664f\u5b30\u7248 CCR5 \u9084\u7121\u6cd5\u597d\u597d\u7684\u8868\u73fe\u5728\u7d30\u80de\u8868\u9762\u3002<\/p>\n

CCR5 \u86cb\u767d\u8cea\u7684\u529f\u80fd\u662f\u4ec0\u9ebc\u5462\uff1f\u7c21\u55ae\u4f86\u8aaa\uff0cCCR5 \u662f T \u7d30\u80de\u8868\u9762\u91cd\u8981\u7684\u8da8\u5316\u56e0\u5b50\u53d7\u9ad4\uff08chemokine receptor\uff09\u4e4b\u4e00\u3002\u8da8\u5316\u56e0\u5b50\u53d7\u9ad4\u50cf\u662f\u5728\u514d\u75ab\u7d30\u80de\u8eab\u4e0a\u88dd\u4e86\u4e00\u500b\u879e\u87fb\u7684\u9f3b\u5b50\u4e00\u6a23\uff0c\u53ea\u8981\u55c5\u5230\u4e86\u4e00\u7d72\u7d72\u8da8\u5316\u56e0\u5b50\uff08chemokine\uff09\u7684\u5b58\u5728\uff0c\u5c31\u50cf\u879e\u87fb\u55c5\u5230\u4e86\u7cd6\u7684\u751c\uff0c\u6703\u9806\u8457\u8da8\u5316\u56e0\u5b50\u7684\u6fc3\u5ea6\u68af\u5ea6\uff0c\u76f4\u76f4\u5f80\u6fc3\u5ea6\u9ad8\u7684\u5730\u65b9\u98db\u5954\u800c\u53bb\u3002CCR5 \u5728\u672a\u7d93\u4e16\u4e8b\u7684 T \u7d30\u80de\u8868\u9762\u4e0d\u592a\u8868\u73fe\uff0c\u4f46\u662f\u53ea\u8981 T \u7d30\u80de\u88ab\u6d3b\u5316\u4e4b\u5f8c\uff0cCCR5 \u5c31\u50cf\u6230\u58eb\u52f3\u7ae0\u4e00\u6a23\uff0c\u6703\u88ab T \u7d30\u80de\u9a55\u50b2\u5730\u4e00\u76f4\u5225\u5728\u9580\u9762\u4e0a\u3002\u800c\u4e14\uff0c\u9664\u4e86 T \u7d30\u80de\uff0cCCR5 \u4e5f\u80fd\u5728\u81ea\u7136\u6bba\u624b\u7d30\u80de\uff08natural killer cells\uff09\u8207\u8a31\u591a\u6297\u539f\u5448\u73fe\u7d30\u80de\u8eab\u4e0a\u627e\u5230\uff1b\u50cf\u662f\u6a39\u7a81\u7d30\u80de\uff08dendritic cells\uff09\u3001\u5de8\u566c\u7d30\u80de\uff08macrophages\uff09\u3002\u514d\u75ab\u7cfb\u7d71\u85c9\u8457\u8abf\u7bc0\u8da8\u5316\u56e0\u5b50\u7684\u7a2e\u985e\uff0c\u5c31\u6709\u5982\u63e1\u4e86\u9059\u63a7\u5668\u5728\u624b\uff0c\u80fd\u50cf\u638c\u63e1\u9059\u63a7\u98db\u6a5f\u4e00\u6a23\u8abf\u7bc0\u7279\u5b9a\u7684 T \u7d30\u80de\u8981\u5f80\u54ea\u88e1\u53bb\u3001\u8a72\u5f80\u54ea\u88e1\u53bb\u3002\u4e0d\u540c\u7684\u8da8\u5316\u56e0\u5b50\u8207\u5176\u53d7\u9ad4\u5728 T \u7d30\u80de\u767c\u80b2\u3001\u6025\u6027\u767c\u708e\u7b49\u60c5\u6cc1\u88e1\uff0c\u90fd\u5404\u81ea\u626e\u6f14\u8457\u91cd\u8981\u89d2\u8272\u3002\u81f3\u4eca\uff0c\u5927\u7d04\u6709\u4e8c\u5341\u7a2e\u8da8\u5316\u56e0\u5b50\u53d7\u9ad4\u3001\u4e94\u5341\u7a2e\u8da8\u5316\u56e0\u5b50\u88ab\u767c\u73fe\u3002<\/p>\n

\u6240\u4ee5\uff0c\u9019\u5169\u500b\u6b50\u6d32\u4eba\u8eab\u4e0a\u6240\u767c\u73fe\u7684 CCR5\u039432<\/em> \u7a81\u8b8a\u57fa\u56e0\u6703\u8b93 CCR5 \u86cb\u767d\u8cea\u7121\u6cd5\u8868\u73fe\u5728\u7d30\u80de\u8868\u9762\uff0c\u7121\u6cd5\u5075\u6e2c\u5230 CCR5 \u76f8\u5c0d\u61c9\u7684\u8da8\u5316\u56e0\u5b50\uff0c\u4e5f\u56e0\u6b64\u5c0d HIV \u75c5\u6bd2\u4e2d\u7684 R5 \u75c5\u6bd2\u682a\u6709 \u8f03\u5f37\u7684\u62b5\u6297\u529b\uff08\u4f46\u4e0d\u662f\u7d55\u5c0d\u7684\u62b5\u6297\u529b\uff09\u3002\u5728\u6b50\u6d32\u8207\u7f8e\u6d32\uff0c\u5927\u6982\u6709 10 % \u7684\u4eba\u5e36\u6709\u4e00\u500b\u7a81\u8b8a\u7684 CCR5-\u039432\uff0c\u50cf\u8ad6\u6587\u4e2d\u90a3\u5169\u500b\u6b50\u6d32\u4eba\u4e00\u6a23\u540c\u6642\u5169\u500b\u7b49\u4f4d\u57fa\u56e0\u90fd\u662f\u7a81\u8b8a CCR5-\u039432 \u57fa\u56e0\u7684\uff0c\u5927\u6982\u53ea\u6709 1 %\u3002\u4f46\u662f\uff0c\u53ea\u5e36\u4e00\u500bCCR5\u039432<\/em> \u7a81\u8b8a\u57fa\u56e0\u7684\u4eba\uff0c\u56e0\u70ba\u53e6\u4e00\u80a1 DNA \u7684\u57fa\u56e0\u5e8f\u5217\u4ecd\u7136\u662f\u7de8\u78bc\u8457\u5b8c\u6574\u7684 CCR5 \u5e8f\u5217\uff0c\u5c0d HIV \u75c5\u6bd2\u4e26\u6c92\u6709\u9632\u8b77\u4f5c\u7528\u3002\u4e0d\u904e\uff0c\u5728\u88ab\u611f\u67d3\u4e4b\u5f8c\uff0c\u75be\u75c5\u60e1\u5316\u7684\u9032\u7a0b\uff0c\u6703\u6bd4\u4e00\u822c\u4eba\u8981\u7a0d\u5fae\u7de9\u6162\u4e00\u4e9b\u3002<\/p>\n

\u4f46\uff0c\u6f14\u5316\u800c\u4f86\u7684\u5929\u4e0b\uff0c\u7d55\u5c0d\u6c92\u6709\u767d\u5403\u7684\u5348\u9910\u3002\u6709\u7814\u7a76\u767c\u73fe\uff0c\u7576\u5169\u500b\u7b49\u4f4d\u57fa\u56e0\u90fd\u662f CCR5\u039432<\/em>\u6642\uff0c\u9019\u4e9b\u4eba\u53cd\u800c\u6bd4\u8f03\u5bb9\u6613\u53d7\u5230\u5176\u4ed6\u75c5\u6bd2\u7684\u611f\u67d3\uff0c\u50cf\u662f\u6d41\u884c\u611f\u5192\u75c5\u6bd2\u3001\u897f\u5c3c\u7f85\u75c5\u6bd2\uff1b\u611f\u67d3\u4e4b\u5f8c\u4e5f\u6bd4\u4e00\u822c\u4eba\u75c5\u60c5\u56b4\u91cd\uff0c\u4e26\u6709\u8f03\u9ad8\u7684\u6b7b\u4ea1\u7387\u3002\u751a\u81f3\uff0cCCR5\u039432<\/em>\u4e5f\u88ab\u767c\u73fe\u662f\u53ef\u80fd\u9020\u6210\u81bd\u56ca\u764c\u8207\u5b50\u5bae\u9838\u764c\u7684\u5371\u96aa\u56e0\u5b50\u4e4b\u4e00\u3002\u4e0d\u5c11\u6d41\u884c\u75c5\u5b78\u7684\u7814\u7a76\u5206\u6790\u4e5f\u5c07\u7cbe\u529b\u6295\u6ce8\u65bc CCR5\u039432 <\/em>\u57fa\u56e0\u8207\u7b2c\u4e8c\u578b\u7cd6\u5c3f\u75c5\u3001\u6c23\u5598\u8207\u591a\u767c\u6027\u786c\u5316\u75c7\u4e4b\u9593\u7684\u95dc\u4fc2\uff0c\u4f46 CCR5\u039432 <\/em>\u57fa\u56e0\u578b\u5c0d\u9019\u4e9b\u75be\u75c5\u7684\u5f71\u97ff\u529b\u4ecd\u672a\u6709\u5341\u8db3\u5b9a\u8ad6\u3002<\/p>\n

\u8abf\u63a7 CCR5 \u6703\u662f\u6cbb\u7652\u5f8c\u5929\u514d\u75ab\u7f3a\u4e4f\u75c7\u5019\u7fa4\u7684\u7d42\u6975\u6b66\u5668\u55ce\uff1f<\/p>\n

2006 \u5e74\u6642\uff0c\u6709\u500b\u88ab HIV \u75c5\u6bd2\u611f\u67d3\u7684\u300c\u67cf\u6797\u75c5\u60a3\u300d Timothy Ray Brown\uff0c\u5728\u6709\u6548\u63a7\u5236\u75c5\u60c5\u5341\u4e00\u5e74\u4e4b\u5f8c\uff0c\u767c\u73fe\u81ea\u5df1\u7f79\u60a3\u6025\u6027\u9aa8\u9ad3\u767d\u8840\u75c5\u3002\u5316\u7642\u5931\u6548\u4e4b\u5f8c\uff0cBrown \u6c7a\u5b9a\u63a5\u53d7\u9020\u8840\u5e79\u7d30\u80de\u9aa8\u9ad3\u79fb\u690d\u3002\u8003\u91cf\u5230\u4ed6\u7684\u75c5\u60c5\uff0c\u52a0\u4e0a\u5e78\u904b\u4e4b\u795e\u7684\u7737\u9867\uff0cBrown \u7684\u91ab\u751f Gero Hutter\u6210\u529f\u70ba\u4ed6\u627e\u5230\u4e00\u4f4d\u5339\u914d\u7684\u9aa8\u9ad3\u6350\u8d08\u8005\uff0c\u5176\u5169\u500b\u7b49\u4f4d\u57fa\u56e0\u90fd\u662f\u7a81\u8b8a\u7684 CCR5\u039432<\/em> \u3002\u800c Brown \u4e5f\u81ea\u6b64\uff0c\u6210\u4e86\u7b2c\u4e00\u500b\u300c\u88ab\u6cbb\u7652\u300d\u7684\u5f8c\u5929\u514d\u75ab\u7f3a\u4e4f\u75c7\u5019\u7fa4\u60a3\u8005\u3002<\/p>\n

\u4f46\u4e4b\u5f8c\u7684\u81e8\u5e8a\u7814\u7a76\u537b\u4e5f\u767c\u73fe\uff0c\u9019\u4f4d\u300c\u67cf\u6797\u75c5\u60a3\u300d\u7684\u50b3\u5947\uff0c\u4e26\u4e0d\u662f\u55ae\u7d14\u9ed1\u9ede\u5230\u9ed1\u9ede\u3001\u5169\u9ede\u9023\u9023\u770b\u90a3\u6a23\u7684\u7c21\u55ae\u3002\u53ef\u80fd\u662f\u5728\u63a5\u53d7\u9aa8\u9ad3\u79fb\u690d\u524d\u7684\u5168\u8eab\u653e\u5c04\u6cbb\u7642\u5f88\u91cd\u8981\uff1b\u53ef\u80fd\u662f\u5728\u63a5\u53d7\u79fb\u690d\u4e4b\u5f8c\u6240\u5f15\u767c\u7684\u79fb\u690d\u7269\u6297\u5bbf\u4e3b\u53cd\u61c9\uff0c\u5c0d\u6d88\u6ec5\u539f\u672c\u9ad4\u5167\u7684\u88ab\u611f\u67d3\u514d\u75ab\u7d30\u80de\u5f88\u91cd\u8981\uff1b\u552f\u4e00\u53ef\u4ee5\u80af\u5b9a\u7684\uff0c\u662f\u5373\u4f7f\u627e\u5230\u4e86\u9069\u5408\u7684 CCR5\u039432 \u9020\u8840\u5e79\u7d30\u80de\u9aa8\u9ad3\u6350\u8d08\u8005\uff0c\u5c31\u9023\u79d1\u5b78\u4e4b\u795e\u4e5f\u7121\u6cd5\u62cd\u80f8\u812f\u4fdd\u8b49\u75c5\u4eba\u4e4b\u5f8c\u4e0d\u6703\u518d\u5fa9\u767c HIV \u611f\u67d3\u3002<\/p>\n

\u4f46\uff0c\u5c31\u7b97\u7121\u6cd5\u62cd\u677f\u5b9a\u6848\u5230\u5e95\u600e\u6a23\u7684\u6cbb\u7642\u7b56\u7565\u624d\u80fd\u91cd\u73fe\u67cf\u6797\u75c5\u60a3\u5947\u8e5f\uff0cCCR5 \u4f9d\u7136\u662f\u5728\u81e8\u5e8a\u8a66\u9a57\u4e2d\uff0c\u8b93\u79d1\u5b78\u5bb6\u8207\u91ab\u7642\u4eba\u54e1\u5728\u4e0d\u65b7\u6478\u9ed1\u524d\u9032\u7684\u904e\u7a0b\u88e1\uff0c\u6700\u9ad8\u9ad8\u639b\u8457\u7684\u9818\u8def\u71c8\u3002\u4e5f\u5df2\u7d93\u6709\u4f5c\u7528\u5728 CCR5 \u7684\u62ee\u6297\u5291\u85e5\u7269\u554f\u4e16\uff1a\u50cf\u662f 2007 \u5e74\u88ab FDA \u6838\u51c6\u4e0a\u5e02\u7684 maraviroc\uff0c\u53ef\u4ee5\u85c9\u7531\u963b\u65b7 CCR5\uff0c\u6709\u6548\u63a7\u5236\u7531HIV R5 \u75c5\u6bd2\u682a\u6240\u9020\u6210\u7684\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u3002\u5176\u4ed6\u6709\u985e\u4f3c\u529f\u80fd\u7684\u5c0f\u5206\u5b50\u85e5\u7269 \uff08vicriviroc\u3001cenicriviroc\uff09\u4e5f\u5728\u4e0d\u540c\u7684\u81e8\u5e8a\u8a66\u9a57\u968e\u6bb5\u3002<\/p>\n

\u601d\u91cf\u6b64\uff0c\u4e0d\u96e3\u60f3\u50cf\u8cc0\u5efa\u594e\u70ba\u4ec0\u9ebc\u6703\u770b\u4e0a CCR5\u3002<\/p>\n

\u8cc0\u5efa\u594e\u4e5f\u4e0d\u662f\u552f\u4e00\u5c0d CCR5 \u6709\u8208\u8da3\u7684\u4eba\u3002\u8a31\u591a\u81e8\u5e8a\u7814\u7a76\uff0c\u4e5f\u5e0c\u671b\u53ef\u4ee5\u85c9\u7531\u8abf\u63a7 CCR5 \u5728\u7d30\u80de\u8868\u9762\u7684\u8868\u73fe\uff0c\u4f86\u63a7\u5236 HIV \u75c5\u6bd2\u7684\u611f\u67d3\u3002\u4f46\u8cc0\u898b\u594e\u4f3c\u4e4e\u60f3\u932f\u4e86\u6700\u91cd\u8981\u7684\u4e00\u4ef6\u4e8b\uff1a\u51a4\u6709\u982d\u3001\u50b5\u6709\u4e3b\uff0c\u9023\u4e0d\u662f\u81ea\u5df1\u7684\u85e5\u90fd\u4e0d\u80fd\u4e82\u5403\uff0c\u5728 CCR5 \u548c\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u5019\u7fa4\u7684\u81e8\u5e8a\u8a66\u9a57\u88e1\uff0c\u5230\u5e95\u8ab0\u624d\u662f\u771f\u6b63\u80fd\u5920\u88ab\u9019\u4e9b\u7814\u7a76\u65b0\u77e5\u6240\u5e6b\u52a9\u5230\u7684\u4eba\u5462\uff1f <\/p>\n

\u6211\u5011\u5148\u4f86\u770b\u770b\u5176\u4ed6\u79d1\u5b78\u5bb6\u600e\u9ebc\u505a\u7684\u597d\u4e86\u3002<\/p>\n

2014 \u5e74\u6642\uff0c\u7531\u7f8e\u570b University of Pennsylvania \u7684 Carl June \u6559\u6388\u6240\u4e3b\u6301\u7684\u4e00\u8d77\u5c0f\u898f\u6a21\u81e8\u5e8a\u8a66\u9a57\uff0c\u60f3\u8981\u63a2\u8a0e\u662f\u4e0d\u662f\u53ef\u4ee5\u5229\u7528\u75c5\u4eba\u81ea\u9ad4\u7684 CD4 T \u7d30\u80de\uff0c\u85c9\u7531\u53e6\u4e00\u7a2e\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u2014\u2014\u92c5\u6307\u6838\u9178\u9176\uff08zinc-finger nucleases\uff09\uff0c\u5c07 CCR5 \u57fa\u56e0\u505a\u66f4\u52d5\uff0c\u4f86\u9054\u5230\u985e\u4f3c\u65bc\u5728 Brown \u75c5\u4eba\u8eab\u4e0a\u7684\u6cbb\u7642\u6548\u679c\u3002\u5229\u7528\u75c5\u4eba\u81ea\u9ad4\u7684 CD4 T \u7d30\u80de\u6709\u500b\u597d\u8655\uff0c\u5373\u4e0d\u7528\u64d4\u5fc3\u514d\u75ab\u6392\u65a5\u7684\u554f\u984c\u3002\u9019\u500b\u81e8\u5e8a\u5be6\u9a57\u7e3d\u5171\u6536\u4e86 12 \u500b HIV \u60a3\u8005\uff0c\u65e8\u5728\u7814\u7a76\u5be6\u9a57\u8a2d\u8a08\u7684\u5b89\u5168\u6027\u8207\u53ef\u884c\u6027\u3002\u7576\u75c5\u4eba\u7684 CCR5 \u57fa\u56e0\u88ab\u7de8\u8f2f\u4e4b\u5f8c\uff0c\u75c5\u4eba\u9ad4\u5167\u7684 HIV \u75c5\u6bd2\u91cf\u5728\u9577\u9054\u4e00\u5e74\u7684\u89c0\u5bdf\u5176\u5167\uff0c\u5747\u7a69\u5b9a\u4e0b\u964d\u3002\u751a\u81f3\u5176\u4e2d\u6709\u4e00\u500b\u75c5\u4eba\u7684\u9ad4\u5167\uff0c\u4e0d\u518d\u5075\u6e2c\u7684\u5230\u75c5\u6bd2\u3002<\/p>\n

\u985e\u4f3c Carl June \u6559\u6388\u7684\u81e8\u5e8a\u5be6\u9a57\u4e5f\u4e0d\u5c11\uff0c\u4f46\uff0c\u90fd\u4e0d\u898b\u6709\u79d1\u5b78\u5bb6\u610f\u5716\u7528\u7cbe\u5375\u7d30\u80de\u4f86\u9054\u5230\u6cbb\u7642\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u3002\u7562\u7adf\uff0cHIV \u75c5\u6bd2\u7684\u653b\u64ca\u5c0d\u8c61\u662f CD4 T \u7d30\u80de\uff0c\u5c31\u7b97\u8981\u5c07 CD4 T \u7d30\u80de\u6253\u6389\u91cd\u7df4\uff0c\u9760\u5e79\u7d30\u80de\u9aa8\u9ad3\u79fb\u690d\u4e5f\u80fd\u5920\u5c07\u75c5\u4eba\u9ad4\u5167\u7684 T \u7d30\u80de\u6c70\u63db\u5982\u65b0\u3002\u50cf Carl June \u6559\u6388\u9019\u6a23\uff0c\u4ee5\u75c5\u4eba\u9ad4\u5167\u7684\u514d\u75ab\u7d30\u80de\u70ba\u4e3b\u9032\u884c\u57fa\u56e0\u7de8\u8f2f\uff0c\u624d\u6b63\u662f\u5c0d\u75c7\u4e0b\u85e5\u3001\u5229\u7528\u6700\u65b0\u7684\u7814\u7a76\u65b0\u77e5\uff0c\u5e6b\u52a9\u5230\u6700\u9700\u8981\u5e6b\u52a9\u7684\u4eba\u3002<\/p>\n

\u800c\u8cc0\u5efa\u594e\u7684\u81e8\u5e8a\u5be6\u9a57\u5462\uff1f\u5728\u4ed6\u4e3b\u5c0e\u7684\u81e8\u5e8a\u8a66\u9a57\u88e1\uff0c\u4e0d\u50c5\u662f\u5728\u53d7\u7cbe\u5375\u7684\u968e\u6bb5\u66f4\u52d5\u4e86 CCR5 \u7684\u57fa\u56e0\u5e8f\u5217\uff0c\u9084\u4e00\u66f4\u52d5\u5c31\u5168\u8eab\u6240\u6709\u7d30\u80de\u90fd\u9023\u5750\uff0c\u66f4\u6703\u907a\u50b3\u5230\u9732\u9732\u8207\u5a1c\u5a1c\u7684\u4e0b\u4e00\u4ee3\u3002\u800c\u4e14\uff0c\u8cc0\u5efa\u594e\u5229\u7528 Cas9\/CRISPR \u6280\u8853\uff0c\u8b93 DNA \u96d9\u80a1\u65b7\u5728\u9760\u8fd1 CCR5\u039432 <\/em>\u7a81\u8b8a\u7684\u4f4d\u7f6e\uff0c\u537b\u6c92\u6709\u6210\u529f\u8b93\u57fa\u56e0\u4fee\u5fa9\u6a5f\u5236\u5c07\u65b7\u88c2\u7684\u5e8f\u5217\uff0c\u4fee\u5fa9\u6210 CCR5\u039432 <\/em>\u7684\u6a23\u5b50\u3002<\/p>\n

\u96d6\u7136\u8aaa\uff0c\u5148\u524d\u7814\u7a76\u666e\u904d\u8a8d\u70ba CCR5\u039432 \u80fd\u5920\u5c0d\u6297 HIV \u75c5\u6bd2\u611f\u67d3\uff0c\u662f\u56e0\u70ba\u88ab\u65b7\u8173\u4e4b\u5f8c\u7684\u77ed\u5c0f\u7248\u86cb\u767d\u8cea\uff0c\u7121\u6cd5\u7a69\u5b9a\u5730\u8868\u73fe\u5728\u7d30\u80de\u8868\u9762\u3002\u4f46\u662f\uff0c\u86cb\u767d\u8cea\u7684\u8abf\u63a7\u672c\u8eab\u5c31\u662f\u4e00\u4ef6\u8907\u96dc\u7684\u4e8b\u3002\u664f\u5b30\u7248 CCR5\u039432 \u6c92\u6709\u6b63\u5e38\u529f\u80fd\uff0c\u537b\u7121\u6cd5\u4e00\u7aff\u5b50\u6253\u7ffb\u4e00\u689d\u8239\uff0c\u4fdd\u8b49\u6240\u6709\u7f3a\u8173\u3001\u77ed\u5c0f\u7248\u7684 CCR5 \u90fd\u4e0d\u4e2d\u7528\uff0c\u90fd\u7121\u6cd5\u5728\u7d30\u80de\u8868\u9762\u7a69\u7a69\u7ad9\u597d\u3002\u8b93\u53d7\u7cbe\u5375\u5167\u7684 CCR5<\/em> \u57fa\u56e0\u5e8f\u5217\u88ab\u4fee\u6539\u6210\u70ba\u5f9e\u672a\u7d93\u7814\u7a76\u904e\u7684\u57fa\u56e0\u5e8f\u5217\uff0c\u672c\u8eab\u5c31\u662f\u4e00\u7a2e\u98a8\u96aa\u3002<\/p>\n

\u8cc0\u5efa\u594e\u6216\u8a31\u662f\u8a8d\u70ba\uff0c\u65e2\u7136 CCR5\u039432 \u7121\u6cd5\u88ab\u8868\u73fe\u5728\u7d30\u80de\u8868\u9762\uff0c\u90a3\u8c48\u4e0d\u5c31\u8ddf\u5254\u9664 CCR5 \u57fa\u56e0\u662f\u6b8a\u9014\u540c\u6b78\u7684\u4e8b\u3002\u4f46\u5982\u679c\u771f\u60f3\u8981\u7259\u4e00\u54ac\u3001\u5fc3\u4e00\u6a6b\uff0c\u4e00\u4e0d\u505a\u4e8c\u4e0d\u4f11\u7684\u628a CCR5<\/em> \u57fa\u56e0\u5254\u9664\u6389\uff0c\u8b93\u86cb\u767d\u8cea\u7d55\u5c0d\u7121\u6cd5\u88ab\u8868\u73fe\uff0c\u901a\u5e38\u4e5f\u6703\u9078\u64c7\u6bd4\u8f03\u9760\u8fd1\u57fa\u56e0\u5e8f\u5217\u524d\u7aef\u7684\u4f4d\u7f6e\uff0c\u8b93 CCR5 \u86cb\u767d\u8cea\u7684\u5408\u6210\u4e00\u88ab\u555f\u52d5\uff0c\u65cb\u5373\u5c31\u78b0\u5230\u7d42\u6b62\u5bc6\u78bc\u5b50\u800c\u88ab\u627c\u6bba\u65bc\u65e9\u671f\uff0c\u4e0d\u6703\u9078\u64c7\u504f\u57fa\u56e0\u4e2d\u5f8c\u7aef\u7684\u4f4d\u7f6e\uff0c\u5f92\u589e\u8b8a\u6578\u3002\u518d\u8005\uff0c\u57fa\u56e0\u5254\u9664 CCR5 \u771f\u80fd\u5920\u50cf CCR5\u039432 \u7684\u5b7f\u751f\u5144\u5f1f\u4e00\u6a23\u55ce\uff1f\u9084\u662f\uff0c\u6703\u4e0d\u6703\u6709\u5176\u4ed6\u8b8a\u6578\u5462\uff1f\u9664\u4e86\u5728\u514d\u75ab\u7d30\u80de\u4e0a\u8868\u73fe\uff0cCCR5 \u4e5f\u53ef\u4ee5\u5728\u5927\u8166\u7d44\u7e54\u88e1\u8a31\u591a\u90e8\u4f4d\u7684\u795e\u7d93\u7d30\u80de\u4e2d\u767c\u73fe\u5176\u8e64\u8de1\u3002\u8fd1\u4f86\uff0c\u4e5f\u6709\u7814\u7a76\u6307\u51fa CCR5 \u5728\u5927\u8166\u767c\u80b2\u4e0a\u53ef\u80fd\u626e\u6f14\u8457\u91cd\u8981\u89d2\u8272\u3002\u5728\u5b30\u5152\u51fa\u751f\u5f8c\u5230\u4e5d\u500b\u6708\u7684\u6642\u9593\u88e1\uff0cCCR5 \u7684\u8868\u73fe\u9010\u6f38\u589e\u52a0\uff0c\u5176\u6d3b\u5316\u5f8c\u7684\u8a0a\u606f\u50b3\u905e\u4e5f\u548c\u795e\u7d93\u7d30\u80de\u7684\u767c\u80b2\u3001\u589e\u751f\u6709\u95dc\u3002\u4e0d\u904e\uff0c\u5728\u5c0f\u9f20\u8eab\u4e0a\u5254\u9664\u6389\u4e86 CCR5 \u57fa\u56e0\u4e4b\u5f8c\uff0c\u5c0f\u9f20\u7684\u5b78\u7fd2\u80fd\u529b\u548c\u8a18\u61b6\u4e5f\u90fd\u88ab\u767c\u73fe\u6709\u6240\u589e\u9032\u3002CCR5 \u5728\u751f\u7406\u529f\u80fd\u4e0a\u7684\u8907\u96dc\u7a0b\u5ea6\uff0c\u4e0d\u662f\u4e00\u53e5\u300c\u70ba\u4e86\u9632\u60a3 HIV \u65bc\u672a\u7136\u300d\u5c31\u80fd\u5920\u8b93\u8cc0\u898b\u594e\u81bd\u5c07\u9019\u6a23\u7684\u81e8\u5e8a\u5be6\u9a57\u4ed8\u8af8\u5be6\u884c\u7684\u4e8b\u3002<\/p>\n

 <\/p>\n

\u539f\u59cb\u8ad6\u6587\uff1a<\/strong><\/p>\n

\u6700\u5148\u767c\u73fe CCR5\u039432 \u80fd\u5920\u589e\u52a0\u5c0d\u6297 HIV \u611f\u67d3\u98a8\u96aa\u7684\u8ad6\u6587\uff1a<\/p>\n

    \n
  1. Paxton WA et al. Relative resistance to HIV-1 infection of CD4 lymphocytes from persons who remain uninfected despite multiple high-risk sexual exposure. Nat Med. 1996 Apr;2(4):412-7.<\/li>\n
  2. Liu R et al. Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell. 1996 Aug 9;86(3):367-77.<\/li>\n<\/ol>\n

    \u63a5\u53d7 CCR5\u039432 \u8840\u6db2\u5e79\u7d30\u80de\u9aa8\u9ad3\u79fb\u690d\u800c\u6cbb\u7652\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u7684\u75c5\u4eba\u6848\u4f8b\uff1aH\u00fctter G et al. Long-term control of HIV by CCR5 Delta32\/Delta32 stem-cell transplantation. N Engl J Med. 2009 Feb 12;360(7):692-8. doi: 10.1056\/NEJMoa0802905.<\/p>\n

     Carl June \u6559\u6388\u5be6\u9a57\u5ba4\u7684CCR5 \u76f8\u95dc\u81e8\u5e8a\u8a08\u756b\uff1aTebas P et a. Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N Engl J Med. 2014 Mar 6;370(10):901-10. doi: 10.1056\/NEJMoa1300662.<\/p>\n

     \u73fe\u5728\u9032\u884c\u4e2d\u7684 CCR5 \u57fa\u56e0\u7de8\u8f2f\u76f8\u95dc\u81e8\u5e8a\u8a08\u756b\uff08\u591a\u662f\u5229\u7528\u92c5\u6307\u6838\u9178\u9176\u6280\u8853\uff09:<\/p>\n

      \n
    1. NCT00842634: Autologous T-Cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases SB-728 for HIV (Zinc-Finger). https:\/\/clinicaltrials.gov\/ct2\/show\/NCT00842634<\/a><\/li>\n
    2. NCT01044654: Phase 1 Dose Escalation Study of Autologous T-cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases in HIV-Infected Patients. https:\/\/clinicaltrials.gov\/ct2\/show\/NCT01044654<\/a><\/li>\n
    3. NCT01252641: Study of Autologous T-cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases in HIV-Infected Subjects. https:\/\/clinicaltrials.gov\/ct2\/show\/NCT01252641<\/a><\/li>\n
    4. NCT01543152: Dose Escalation Study of Cyclophosphamide in HIV-Infected Subjects on HAART Receiving SB-728-T. https:\/\/clinicaltrials.gov\/ct2\/show\/NCT01543152<\/a><\/li>\n
    5. NCT02225665: Repeat Doses of SB-728mR-T After Cyclophosphamide Conditioning in HIV-Infected Subjects on HAART. https:\/\/clinicaltrials.gov\/ct2\/show\/NCT02225665<\/li>\n
    6. NCT02388594: A Phase I Study of T-Cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases SB-728mR in HIV-Infected Patients. https:\/\/clinicaltrials.gov\/ct2\/show\/NCT02388594<\/li>\n
    7. NCT02500849: Safety Study of Zinc Finger Nuclease CCR5-modified Hematopoietic Stem\/Progenitor Cells in HIV-1 Infected Patients. https:\/\/clinicaltrials.gov\/ct2\/show\/NCT02500849<\/a><\/li>\n<\/ol>\n

      \u5254\u9664 CCR5 \u57fa\u56e0\u53ef\u80fd\u589e\u9032\u5c0f\u9f20\u5728\u5b78\u7fd2\u8207\u8a18\u61b6\u4e2d\u7684\u8868\u73fe\uff1aZhou M et al. CCR5 is a suppressor for cortical plasticity and hippocampal learning and memory. Elife. 2016 Dec 20;5. pii: e20985. doi: 10.7554\/eLife.20985.<\/p>\n

       \u53c3\u8003\u8cc7\u6599\uff1a<\/strong><\/p>\n

        \n
      1. Cornu TI et al. Refining strategies to translate genome editing to the clinic. Nat Med. 2017 Apr 3;23(4):415-423. doi: 10.1038\/nm.4313.<\/li>\n
      2. Venuti A et al. The Role of Natural Antibodies to CC Chemokine Receptor 5 in HIV Infection. Front Immunol. 2017 Oct 30;8:1358. doi: 10.3389\/fimmu.2017.01358. eCollection 2017.<\/li>\n
      3. Martin-Blondel G et al. CCR5 blockade for neuroinflammatory diseases--beyond control of HIV. Nat Rev Neurol. 2016 Feb;12(2):95-105. doi: 10.1038\/nrneurol.2015.248. Epub 2016 Jan 18.<\/li>\n
      4. Sorce S et al. The chemokine receptor CCR5 in the central nervous system. Prog Neurobiol. 2011 Feb;93(2):297-311. doi: 10.1016\/j.pneurobio.2010.12.003. Epub 2010 Dec 14. Review.<\/li>\n
      5. Barmania F et al. C-C chemokine receptor type five (CCR5): An emerging target for the control of HIV infection. Appl Transl Genom. 2013 May 26;2:3-16. eCollection 2013 Dec 1.<\/li>\n<\/ol>\n

         <\/p>\n

        --<\/strong>
        \n\u4f5c\u8005\uff1a\u99f1\u5b9b\u7433\u3000\u7f8e\u570b\u8056\u8def\u6613\u83ef\u76db\u9813\u5927\u5b78\uff08Washington University in St. Louis\uff09\u514d\u75ab\u5b78\u535a\u58eb\uff0c\u5f9e\u4e8bT\u7d30\u80de\u767c\u80b2\u8207\u6d3b\u5316\u76f8\u95dc\u7684\u8a0a\u606f\u50b3\u5c0e\u7814\u7a76\u3002<\/strong><\/p>\n

        \"\u52a0\u5165\u597d\u53cb\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

        \u25a0\u7b2c\u4e00\u5c0d\u57fa\u56e0\u7de8\u8f2f\u5bf6\u5bf6\uff0c\u5c31\u9019\u6a23\u5728\u773e\u4eba\u7684\u9a5a\u6115\u4e2d\u8a95\u751f\u4e86\u3002\u4e2d\u570b\u5357\u65b9\u79d1\u6280\u5927\u5b78\u526f\u6559\u6388\u8cc0\u5efa\u594e\uff0c\u5229\u7528 CRISPR\/Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u5c07\u9019\u5c0d\u96d9\u80de\u80ce\u9ad4\u5167\u7684 CCR5 \u57fa\u56e0\u5254\u9664\uff0c\u5e0c\u671b\u9019\u5c0d\u96d9\u80de\u80ce\u7d42\u5176\u4e00\u751f\u90fd\u4e0d\u7528\u64d4\u5fc3\u88ab HIV \u75c5\u6bd2\u611f\u67d3\u3002\u8cc0\u5efa\u594e\u7684\u7406\u7531\uff0c\u5728\u79d1\u5b78\u502b\u7406\u524d\u88ab\u75db\u6279\u7684\u9ad4\u7121\u5b8c\u819a\uff1b\u90a3\u5728\u79d1\u5b78\u5b78\u7406\u9762\u524d\u5462\uff1f\u771f\u5982\u4ed6\u6240\u8a00\u4e00\u822c\uff0c\u7ad9\u5f97\u4f4f\u8173\u55ce\uff1f<\/p>\n","protected":false},"author":20,"featured_media":33364,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,1025],"tags":[4119,3075,256,4018,4120,4114],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2018\/12\/pic.jpeg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/33362"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33362"}],"version-history":[{"count":4,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/33362\/revisions"}],"predecessor-version":[{"id":33371,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/33362\/revisions\/33371"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/33364"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33362"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33362"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33362"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}