{"id":33336,"date":"2018-12-07T00:59:02","date_gmt":"2018-12-06T16:59:02","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=33336"},"modified":"2019-07-31T11:07:35","modified_gmt":"2019-07-31T03:07:35","slug":"%e3%80%90crispr-baby-%e7%b3%bb%e5%88%97%e4%b9%8b%e4%b8%80%e3%80%91%e9%9c%b2%e9%9c%b2%e5%92%8c%e5%a8%9c%e5%a8%9c%ef%bc%8c%e9%a1%98%e4%bd%a0%e5%80%91%e4%b8%80%e5%ae%9a%e8%a6%81%e5%b9%b3%e5%ae%89","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=33336","title":{"rendered":"\u3010CRISPR baby \u7cfb\u5217\u4e4b\u4e00\u3011\u9732\u9732\u548c\u5a1c\u5a1c\uff0c\u9858\u4f60\u5011\u4e00\u5b9a\u8981\u5e73\u5b89\u53c8\u5065\u5eb7\u7684\u9577\u5927"},"content":{"rendered":"<p style=\"text-align: left;\"><span style=\"color: #333399;\"><strong>\u25a0\u6211\u60f3\uff0c\u5230\u4e86\u5f88\u591a\u5e74\u5f88\u591a\u5e74\u4e4b\u5f8c\uff0c2018 \u5e74\u7684\u5341\u4e00\u6708\uff0c\u90fd\u6703\u8b93\u4eba\u8a18\u5f97\u3002\u9019\u500b\u6708\uff0c\u4e00\u5c0d\u96d9\u80de\u80ce\u5973\u5b30\uff0c\u88ab\u9019\u6642\u5019\u7684\u6211\u5011\u559a\u540d\u70ba\u9732\u9732\u8ddf\u5a1c\u5a1c\u3002\u56e0\u70ba\u4e00\u500b\u79d1\u5b78\u81e8\u5e8a\u8a66\u9a57\uff0c\u5979\u5011\u7684\u907a\u50b3\u57fa\u56e0\u88ab\u7ac4\u6539\uff0c\u5c31\u9019\u6a23\u50cf\u767d\u8001\u9f20\u4e00\u6a23\uff0c\u5471\u5471\u589c\u4e86\u5730\u3002\u89aa\u611b\u7684\u9732\u9732\u548c\u5a1c\u5a1c\uff0c\u6b77\u53f2\u7121\u6cd5\u88ab\u6539\u5beb\uff0c\u4f46\u5e0c\u671b\u9019\u5b87\u5b99\u88e1\u7684\u4ec1\u539a\uff0c\u9019\u4e16\u9593\u88e1\u70ba\u4f60\u5011\u4e0d\u5e73\u3001\u4e0d\u6368\u7684\u6bcf\u4efd\u5fc3\u610f\uff0c\u90fd\u80fd\u8b93\u9001\u5b50\u9ce5\u4e00\u8d77\u5e36\u5230\u4f60\u5011\u8eab\u908a\uff0c\u8b93\u4f60\u5011\u63a5\u4e0b\u4f86\u7684\u4e00\u751f\uff0c\u5065\u5eb7\u53c8\u5e73\u5b89<span style=\"color: #333399;\">\u3002<\/span><\/strong><\/span><\/p>\n<figure id=\"attachment_33337\" aria-describedby=\"caption-attachment-33337\" style=\"width: 450px\" class=\"wp-caption alignleft\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-33337\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2018\/12\/47085995_2256292061049744_1519136843197579264_o.jpg\" alt=\"\" width=\"450\" height=\"312\" data-wp-pid=\"33337\"><figcaption id=\"caption-attachment-33337\" class=\"wp-caption-text\">\u5716\u7247\u4f86\u6e90:UCSF<\/figcaption><\/figure>\n<p><strong>\u64b0\u6587\uff5c\u99f1\u5b9b\u7433<\/strong><\/p>\n<p>\u8cc0\u5efa\u594e\uff0c\u5e74\u4e09\u5341\u56db\uff0c\u7559\u8077\u505c\u85aa\u4e2d\u570b\u5357\u65b9\u79d1\u6280\u5927\u5b78\u526f\u6559\u6388\uff0c\u5728 2018 \u5e74\u5341\u4e00\u6708\u7684\u6700\u5f8c\u4e00\u500b\u9031\u672b\uff0c\u9707\u64bc\u4e86\u5168\u7403\u793e\u6703\u8207\u79d1\u5b78\u754c\u3002\u8cc0\u5148\u751f\u505a\u4e86\u4ec0\u9ebc\uff1f\u4ed6\u5229\u7528 CRISPR\/Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u7ac4\u5beb\u4e86\u4eba\u985e\u80da\u80ce\u57fa\u56e0\u9ad4\u5e8f\u5217\u3002\u4ed6\u4e0d\u662f\u70ba\u4e86\u6cbb\u7642\u7f55\u898b\u7684\u3001\u8b93\u4eba\u675f\u624b\u7121\u7b56\u7684\u907a\u50b3\u6027\u75be\u75c5\uff0c\u4ed6\u8aaa\u4ed6\u60f3\u8981\u8b93\u4eba\u985e\u514d\u65bc HIV \u75c5\u6bd2\u3002\u4ed6\u89ba\u5f97\u9732\u9732\u548c\u5a1c\u5a1c\u7684\u7238\u7238\u662f HIV \u5e36\u539f\u8005\u5f88\u53ef\u6190\uff0c\u60f3\u8981\u7d66\u53d7\u632b\u65bc\u985e\u4f3c\u60c5\u6cc1\u7684\u7238\u7238\u5011\u4e00\u500b\u5e0c\u671b\u3002\u65bc\u662f\uff0c\u4ed6\u9818\u982d\u4e86\u4e00\u7d44\u81e8\u5e8a\u5be6\u9a57\u8a08\u756b\uff0c\u610f\u5728\u5229\u7528\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u8b93\u9019\u4e9b\u5bb6\u5ead\u7684\u5f8c\u4ee3\uff0c\u80fd\u5920\u6709\u500b\u300c\u514d\u75ab\u300d\u65bc HIV \u75c5\u6bd2\u611f\u67d3\u7684\u5b69\u5b50\u3002<\/p>\n<p>\u4ed6\u628a\u9019\u81e8\u5e8a\u5be6\u9a57\u8a08\u756b\u5b9a\u8abf\u70ba\u7814\u767c\u51fa\u80fd\u5920\u5c0d\u6297 HIV \u75c5\u6bd2\u7684\u300c\u75ab\u82d7\u300d\uff0c\u53ea\u662f\u4ee5\u8b93\u5168\u7403\u79d1\u5b78\u5bb6\u6b32\u54ed\u7121\u6dda\u3001\u75ab\u82d7\u4e4b\u7236\u91d1\u7d0d\u535a\u58eb\u80fd\u5f9e\u58b3\u5893\u88e1\u8df3\u8d77\u4f86\u7684\u624b\u6bb5\uff0c\u4f86\u9054\u6210\u4ed6\u7684\u5be6\u9a57\u76ee\u7684\u3002\u4ed6\u89ba\u5f97\uff0c\u65e2\u7136\u5927\u5bb6\u90fd\u8aaa HIV \u75c5\u6bd2\u611f\u67d3\u514d\u75ab\u7d30\u80de T \u7d30\u80de\uff0c\u800c\u4e14 HIV \u75c5\u6bd2\u5f97\u9760\u7d30\u80de\u8868\u9762\u7684\u53d7\u9ad4 CCR5 \u9032\u5165\u7d30\u80de\uff0c\u90a3\u5c31\u5229\u7528\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u88fd\u9020\u51fa\u5168\u8eab\u4e0a\u4e0b\u6bcf\u4e00\u500b\u7d30\u80de\u90fd\u6c92\u6709\u8fa6\u6cd5\u8868\u73fe CCR5 \u86cb\u767d\u8cea\u7684\u4eba\u985e\uff1b\u90a3\u9019\u500b\u65b0\u4eba\u985e\uff0c\u4e0d\u662f\u5c31\u7d42\u5176\u4e00\u751f\u90fd\u4e0d\u7528\u5bb3\u6015\u88ab HIV \u75c5\u6bd2\u611f\u67d3\u4e86\u55ce\uff1f<\/p>\n<p>\u9019\u8ad6\u9ede\u7684\u932f\uff0c\u591a\u5c64\u6b21\u5230\u9023\u6211\u6700\u611b\u7684\u62b9\u8336\u5343\u5c64\u86cb\u7cd5\u90fd\u8981\u7518\u62dc\u4e0b\u98a8\u3002\u4ec0\u9ebc\u662f\u75ab\u82d7\uff1f\u75ab\u82d7\u6709\u9ede\u50cf\u662f\u7279\u5225\u5beb\u7d66\u514d\u75ab\u7cfb\u7d71\u770b\uff0c\u597d\u8b93\u514d\u75ab\u7cfb\u7d71\u80fd\u5920\u5c0d\u4ed8\u5927\u5343\u4e16\u754c\u88e1\uff0c\u67d0\u4e9b\u80fd\u5920\u5f15\u8d77\u56b4\u91cd\u50b3\u67d3\u75c5\u7684\u5fae\u751f\u7269\u7684\u5c0f\u6284\u3002\u514d\u75ab\u7cfb\u7d71\u521d\u898b\u81f4\u75c5\u5fae\u751f\u7269\u5165\u4fb5\u7684\u6642\u5019\uff0c\u50cf\u662f\u521d\u6b21\u4e0a\u6230\u5834\u7684\u5c0f\u5175\uff0c\u53cd\u61c9\u6709\u9ede\u61a8\u3001\u52d5\u4f5c\u6709\u9ede\u6162\uff1b\u4f46\u9019\u4e9b\u5c0f\u5175\u4e26\u4e0d\u50bb\uff0c\u6709\u4e86\u7b2c\u4e00\u6b21\u6bba\u6575\u7d93\u9a57\u4e4b\u5f8c\uff0c\u4e0b\u6b21\u518d\u770b\u5230\u540c\u6a23\u7684\u81f4\u75c5\u5fae\u751f\u7269\uff0c\u514d\u75ab\u7d30\u80de\u5c31\u80fd\u5920\u65e2\u5feb\u901f\u53c8\u795e\u529b\u7121\u6cd5\u64cb\u3001\u5236\u4f0f\u5165\u4fb5\u7684\u5916\u4f86\u5fae\u751f\u7269\u3002\u800c\u75ab\u82d7\u5c31\u50cf\u662f\u6230\u60c5\u60c5\u5831\u5ba4\u88e1\u7684\u91cd\u8981\u8ecd\u60c5\uff0c\u8b93\u514d\u75ab\u7d30\u80de\u5148\u8ddf\u9019\u4e9b\u7d93\u904e\u7279\u6b8a\u8655\u7406\u904e\u7684\u5916\u4f86\u5fae\u751f\u7269\u6253\u500b\u7167\u9762\uff0c\u9632\u885b\u6f14\u7fd2\u4e00\u4e0b\uff1b\u4e0b\u6b21\u518d\u771f\u69cd\u5be6\u5f48\u51a4\u5bb6\u78b0\u982d\u7684\u6642\u5019\uff0c\u514d\u75ab\u7cfb\u7d71\u5c31\u77e5\u9053\u61c9\u8a72\u5982\u4f55\u70ba\u500b\u9ad4\u63d0\u4f9b\u7d55\u4f73\u9632\u79a6\u4e86\u3002<\/p>\n<p>CRISPR\/Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u7684\u7814\u767c\uff0c\u6700\u521d\u4e5f\u7684\u78ba\u662f\u53d7\u5230\u7d30\u83cc\u514d\u75ab\u7cfb\u7d71\u7684\u555f\u8fea\uff0c\u4f46\u537b\u8ddf\u75ab\u82d7\u662f\u5b8c\u5168\u642d\u4e0d\u4e0a\u908a\u3002\u7d30\u83cc\u8207\u53e4\u7d30\u83cc\u70ba\u4e86\u9632\u79a6\u566c\u83cc\u9ad4\u4e00\u800c\u518d\u5730\u5165\u4fb5\uff0c\u6703\u628a\u66fe\u7d93\u5165\u4fb5\u904e\u7684\u566c\u83cc\u9ad4 DNA \u7247\u6bb5\u5e8f\u5217\u6536\u96c6\u8d77\u4f86\uff0c\u7de8\u5217\u6210\u518a\uff0c\u50cf\u662f\u70ba\u6bcf\u4e00\u7a2e\u566c\u83cc\u9ad4\u90fd\u88dd\u4e86\u7279\u6b8a\u7684\u7d05\u5916\u7dda\u611f\u6e2c\u5668\u3002\u566c\u83cc\u9ad4\u53ea\u8981\u5165\u4fb5\u904e\u5c31\u6703\u7559\u4e0b\u75d5\u8de1\uff1b\u7576\u566c\u83cc\u9ad4\u518d\u6b21\u5165\u4fb5\u6642\uff0c\u5c31\u6703\u8b66\u5831\u5927\u97ff\u3002Cas \u86cb\u767d\u8cea\u5c31\u6703\u767c\u52d5\u5176\u8eab\u70ba DNA \u6838\u9178\u5167\u5207\u9176\u7684\u672c\u6027\uff0c\u5c07\u5165\u4fb5\u7684\u566c\u83cc\u9ad4 DNA \u5206\u89e3\u6389\u3002Cas \u86cb\u767d\u50cf\u662f\u611b\u5fb7\u83ef\u526a\u5200\u624b\u3002\u800c\u90a3\u4e9b\u88ab\u7d30\u83cc\u7559\u8d77\u4f86\u7684\u566c\u83cc\u9ad4 DNA \u7247\u6bb5\uff0c\u5728\u88ab\u8f49\u9304\u6210 crRNA \u4e4b\u5f8c\uff0c\u50cf\u662f\u53ef\u4ee5\u88dd\u5728 Cas \u86cb\u767d\u8cea\u4e0a\u7684 GPS \u5b9a\u4f4d\u7cfb\u7d71\uff0c\u80fd\u5920\u5e6b\u52a9 Cas \u86cb\u767d\u8cea\u627e\u5230\u9700\u8981\u5927\u526a\u5200\u5580\u5693\u3001\u597d\u9032\u884c\u57fa\u56e0\u7de8\u8f2f\u7684 DNA \u4f4d\u7f6e\u3002<\/p>\n<p>\u79d1\u5b78\u5bb6\u5728\u767c\u73fe\u7d30\u83cc\u5229\u7528\u566c\u83cc\u9ad4\u82b1\u540d\u518a\u7684\u65b9\u5f0f\uff0c\u4f86\u8b93 Cas \u86cb\u767d\u57f7\u884c\u300cDNA \u5927\u65b7\u88c2\u300d\u4efb\u52d9\u6642\uff0c\u5c31\u60f3\u5230\u662f\u4e0d\u662f\u53ef\u4ee5\u85c9\u8457\u985e\u4f3c\u7684\u65b9\u6cd5\uff0c\u628a\u79d1\u5b78\u5bb6\u60f3\u8981\u66f4\u52d5\u7684\u57fa\u56e0\u5e8f\u5217\u4e5f\u4f9d\u6a23\u756b\u846b\u8606\u5730\u7de8\u8f2f\u6210\u518a\uff0c\u8b93 Cas \u86cb\u767d\u53bb\u7834\u58de\u8a72\u6bb5\u5e8f\u5217\u3002Cas \u86cb\u767d\u6839\u64da\u7d30\u83cc\u7a2e\u985e\u4e0d\u540c\uff0c\u6709\u8a31\u591a\u7a2e\u3002\u6700\u5e38\u88ab\u4f7f\u7528\u65bc\u54fa\u4e73\u985e\u52d5\u7269\u8207\u7d30\u80de\u57fa\u56e0\u7de8\u8f2f\u7684\u662f Cas9 \u86cb\u767d\u3002<\/p>\n<p>\u7d30\u83cc\u85c9\u7531 Cas \u86cb\u767d\u6bba\u566c\u83cc\u9ad4\u65bc\u7121\u5f62\uff0c\u79d1\u5b78\u5bb6\u5012\u4e0d\u5e0c\u671b Cas9 \u86cb\u767d\u5982\u6b64\u72e0\u7d55\uff0c\u800c\u662f\u5e0c\u671b\u7576 Cas9 \u86cb\u767d\u7684\u5927\u526a\u5200\u5728\u60f3\u8981\u4fee\u6b63\u7684 DNA&nbsp; \u5e8f\u5217\u4e0a\u5580\u5693\u4e4b\u5f8c\uff0c\u54fa\u4e73\u985e\u52d5\u7269\u7d30\u80de\u80fd\u5920\u555f\u52d5 DNA \u4fee\u5fa9\u6a5f\u5236\uff0c\u5c07\u65b7\u88c2\u7684 DNA \u518d\u597d\u597d\u7e2b\u88dc\u8d77\u4f86\u3002\u800c\u5728\u7e2b\u88dc\u904e\u7a0b\u88e1\uff0c\u4e5f\u63d0\u4f9b\u79d1\u5b78\u5bb6\u300c\u99ed\u300d\u9032\u57fa\u56e0\u5e8f\u5217\u7684\u6a5f\u6703\u3002\u5982\u679c\uff0c\u9019\u6642\u5019\u79d1\u5b78\u5bb6\u80fd\u5920\u7d66\u4e88\u8a72\u54fa\u4e73\u985e\u7d30\u80de\u4e00\u6bb5 DNA \u5e8f\u5217\u4f5c\u70ba\u300c\u6a21\u677f\u300d\uff0c\u54fa\u4e73\u985e\u7d30\u80de\u5c31\u80fd\u5920\u6bd4\u7167\u6a21\u677f\u4e0a\u7684\u82b1\u8272\uff0c\u62fc\u88dc\u51fa\u79d1\u5b78\u5bb6\u60f3\u8981\u7684 DNA \u5e8f\u5217\u6a21\u6a23\u3002\u5982\u679c\u6c92\u6709\u63d0\u4f9b\u984d\u5916\u7684 DNA \u4fee\u5fa9\u6a21\u677f\uff0c\u90a3\u51fa\u4f86\u7684\u6210\u54c1\u5c31\u6703\u50cf\u662f\u7528\u5b87\u5b99\u7684\u9ab0\u5b50\u96a8\u6a5f\u6c7a\u5b9a\u57fa\u56e0\u4fee\u88dc\u6a5f\u5236\u7684\u4e0b\u91dd\u8655\uff0c\u6210\u54c1\u4e0a\u7684\u91dd\u8173\u843d\u9ede\u662f\u4e94\u82b1\u516b\u9580\uff0c\u5404\u6709\u5343\u79cb\u3002\u4f46\uff0c\u5982\u6b64\u4fee\u88dc\u51fa\u4f86\u7684 DNA\uff0c\u5341\u6709\u516b\u4e5d\u90fd\u7121\u6cd5\u8868\u73fe\u51fa\u5177\u6709\u6b63\u5e38\u529f\u80fd\u7684\u86cb\u767d\u8cea\u3002\u5229\u7528 CRISPR\/Cas9 \u4f86\u9032\u884c\u57fa\u56e0\u7de8\u8f2f\u7684\u76f8\u95dc\u7814\u7a76\u9817\u591a\uff0c\u5927\u591a\u6578\u7684\u7814\u7a76\u90fd\u5e0c\u671b\u80fd\u5920\u70ba\u56b4\u91cd\u7684\u907a\u50b3\u6027\u75be\u75c5\uff0c\u63d0\u4f9b\u4e00\u7dda\u751f\u6a5f\u3002<\/p>\n<p>\u4f46\u8cc0\u5efa\u594e\u6709\u8208\u8da3\u7684\u662f\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u5019\u7fa4\u3002\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u5019\u7fa4\u8087\u56e0\u65bc HIV \u75c5\u6bd2\u7684\u611f\u67d3\uff1bHIV \u75c5\u6bd2\u55dc CD4 T \u7d30\u80de\uff0c\u5728\u611f\u67d3\u7684\u904e\u7a0b\u4e2d\uff0c\u9664\u4e86 CD4\uff0c\u9084\u9700\u8981\u53e6\u4e00\u500b CCR5 \u86cb\u767d\u7684\u5e6b\u52a9\uff08\u4f46\u4e5f\u6709\u5229\u7528 CXCR4 \u7684 HIV \u75c5\u6bd2\uff09\u3002<em>CCR5<\/em> \u662f\u500b\u6eff\u8eab\u6545\u4e8b\u7684\u57fa\u56e0\u3002\u5728\u4eba\u985e\u7fa4\u88e1\uff0c\u88ab\u767c\u73fe\u7684 <em>CCR5<\/em> \u57fa\u56e0\u591a\u6a23\u6027\u4e0d\u5c11\uff0c\u800c\u5176\u4e2d\u6709\u4e00\u500b\u591a\u6a23\u6027 <em>CCR5<\/em><em>\u039432<\/em> \u66f4\u662f\u773e\u6240\u77da\u76ee\u7684\u7126\u9ede\u3002\u5982\u679c\u5169\u7d44 <em>CCR5<\/em> \u57fa\u56e0\u300c\u90fd\u300d\u5e36\u6709 <em>CCR5<\/em><em>\u039432<\/em>\uff0c \u9019\u4e9b\u4eba\u56e0\u70ba CD4 T \u7d30\u80de\u4e0a\u7121\u6cd5\u8868\u73fe\u6709\u529f\u80fd\u7684\u86cb\u767d\uff0c\u5c0d HIV \u75c5\u6bd2\u7684\u62b5\u6297\u529b\u6bd4\u8f03\u9ad8\u3002\u4f46\u9019\u7fa4\u4eba\u4e5f\u4e0d\u662f\u5c0d\u9700\u8981 CCR5 \u7684 HIV \u75c5\u6bd2\u7d55\u5c0d\u514d\u75ab\u3001\u5c0d\u65bc\u9700\u8981 CXCR4 \u7684\u75c5\u6bd2\u66f4\u662f\u5b8c\u5168\u6c92\u8f4d\u3002\u540c\u6642\u5e36\u6709\u5169\u7d44 <em>CCR5<\/em><em>\u039432<\/em> \u57fa\u56e0\u7684\u4eba\u6975\u5c11\uff0c\u5728\u6b50\u7f8e\u5927\u7d04\u53ea\u6709 1 %\uff0c\u5728\u975e\u6d32\u3001\u4e9e\u6d32\u4eba\u88e1\u66f4\u662f\u6c92\u6709\u88ab\u767c\u73fe\u904e\u3002<\/p>\n<p>\u8cc0\u5efa\u594e\u6253\u7b97\u628a <em>CCR5<\/em> \u9019\u7d44\u57fa\u56e0\u7a81\u8b8a\u70ba\u50cf\u662f <em>CCR5<\/em><em>\u039432<\/em> \u7684\u6a23\u5b50\uff0c\u8b93 CCR5 \u86cb\u767d\u7121\u6cd5\u8868\u73fe\uff0c\u85c9\u6b64\u963b\u6493 HIV \u75c5\u6bd2\u7684\u611f\u67d3\u3002\u5728\u8cc0\u5efa\u594e\u6240\u8ca0\u8cac\u7684\u9019\u8d77\u81e8\u5e8a\u8a66\u9a57\u88e1\uff0c\u4ed6\u62db\u52df\u4e86\u516b\u5c0d\u592b\u59bb\uff0c\u7238\u7238\u7686\u611f\u67d3 HIV \u75c5\u6bd2\uff0c\u4f46\u5abd\u5abd\u90fd\u5065\u5eb7\u3001\u6c92\u6709\u88ab\u611f\u67d3\u7d00\u9304\u3002<\/p>\n<p>\u8cc0\u5efa\u594e\u9818\u982d\u7684\u81e8\u5e8a\u5be6\u9a57\uff0c\u4e26\u4e0d\u662f\u70ba\u4e86\u4fdd\u8b77\u80da\u80ce\u5728\u61f7\u5b55\u904e\u7a0b\u4e2d\u4e0d\u88ab HIV \u75c5\u6bd2\u611f\u67d3\u3002\u5728\u8a66\u9a57\u904e\u7a0b\u4e2d\uff0c\u5728\u9032\u884c\u9ad4\u5916\u4eba\u5de5\u53d7\u5b55\u4e4b\u524d\uff0c\u7238\u7238\u7684\u7cbe\u5b50\u5df2\u7d93\u7d93\u904e\u7cbe\u5b50\u6d17\u6ecc\uff08sperm wash\uff09\uff0c\u800c\u9019\u9053\u6b65\u9a5f\u5df2\u4e00\u800c\u518d\u3001\u518d\u800c\u4e09\u7684\u88ab\u9a57\u8b49\uff0c\u8db3\u4ee5\u4fdd\u8b77\u80ce\u5152\u514d\u53d7 HIV \u75c5\u6bd2\u611f\u67d3\u7684\u5a01\u8105\u3002\u8cc0\u5efa\u594e\u7684\u76ee\u6a19\uff0c\u662f\u5229\u7528\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u8b93\u9019\u4e9b\u5bf6\u5bf6\u65e5\u5f8c\u4e0d\u6703\u6709\u88ab HIV \u75c5\u6bd2\u611f\u67d3\u7684\u53ef\u80fd\u3002<\/p>\n<p>\u5728\u81e8\u5e8a\u5be6\u9a57\u904e\u7a0b\u4e2d\uff0c\u4ee5\u9ad4\u5916\u4eba\u5de5\u53d7\u7cbe\u6280\u8853\u70ba\u672c\uff0c\u8cc0\u5efa\u594e\u7684\u7814\u7a76\u5718\u968a\u53ef\u4ee5\u5728\u7cbe\u5375\u53d7\u7cbe\u6642\uff0c\u5c07 Cas9 \u86cb\u767d\u5148\u548c\u5176\u76f8\u95dc\u96f6\u4ef6\uff08\u4f8b\u5982\uff1a\u53ef\u4ee5\u5e6b\u52a9 Cas9 \u627e\u5230 <em>CCR5<\/em> \u57fa\u56e0\u7684 crRNA\uff09\u7d44\u5408\u8d77\u4f86\u5f8c\uff0c\u5229\u7528\u986f\u5fae\u6ce8\u5c04\u6280\u8853\uff0c\u6ce8\u5c04\u5230\u53d7\u7cbe\u5375\u5167\u3002\u5728\u8a66\u9a57\u904e\u7a0b\u4e2d\uff0c\u4e00\u5c0d\u592b\u59bb\u4e2d\u9014\u9000\u51fa\u3002\u5728\u8cc0\u5efa\u594e\u6f14\u8b1b\u7684\u6642\u5019\uff0c\u8868\u793a\u7e3d\u5171\u6709 31 \u500b\u80da\u80ce\u767c\u80b2\u81f3\u56ca\u80da\uff1b\u5176\u4e2d\uff0c\u7d04\u4e03\u6210\u7684\u80da\u80ce\uff0c\u5176\u57fa\u56e0\u6709\u88ab\u7de8\u8f2f\u904e\u7684\u75d5\u8de1\u3002\u5f8c\u4f86\uff0c<em>Nature&nbsp;<\/em>\u671f\u520a\u5728\u8cc0\u5efa\u594e\u6f14\u8b1b\u4e4b\u5f8c\uff0c\u548c\u5176\u767c\u8a00\u4eba\u806f\u7e6b\uff0c\u767c\u8a00\u4eba\u8868\u793a\u5728\u6b64\u8a66\u9a57\u88e1\uff0c\u7e3d\u5171\u6709 22 \u500b\u80da\u80ce\uff0c\u5176\u4e2d 16 \u500b\u80da\u80ce\u5065\u5eb7\u3001\u4e14\u57fa\u56e0\u5e8f\u5217\u6709\u88ab\u6210\u529f\u7de8\u8f2f\u3002\u800c\u5728\u9019\u4e9b\u80da\u80ce\u88e1\uff0c\u9732\u9732\u8207\u5a1c\u5a1c\u7684\u6bcd\u89aa\u7e3d\u5171\u6709\u56db\u500b\u80da\u80ce\uff0c\u5176\u4e2d\u5169\u500b\u57fa\u56e0\u5e8f\u5217\u88ab\u6210\u529f\u7de8\u8f2f\uff08\u4f46\u5176\u4e2d\u4e00\u500b\u80da\u80ce\u4ecd\u5e36\u6709\u5b8c\u6574\u3001\u6b63\u5e38\u7684 <em>CCR5<\/em> \u57fa\u56e0\u5e8f\u5217\uff09\u3002\u9019\u5169\u500b\u80da\u80ce\u88ab\u690d\u5165\u3001\u6700\u7d42\u8a95\u751f\u4e86\u9732\u9732\u8207\u5a1c\u5a1c\u9019\u5c0d\u96d9\u80de\u80ce\u3002\u53e6\u5916\uff0c\u9084\u6709\u4e00\u4f4d\u5abd\u5abd\u61f7\u5b55\u4e2d\uff0c\u4f46\u56e0\u70ba\u9084\u5728\u6975\u70ba\u521d\u671f\u7684\u968e\u6bb5\uff0c\u6240\u4ee5\u9084\u7121\u6cd5\u78ba\u8a8d\u662f\u4e0d\u662f\u9084\u6709\u4e0b\u4e00\u500b\u57fa\u56e0\u5bf6\u5bf6\u3002<\/p>\n<p>\u5728\u8cc0\u5efa\u594e\u7684\u6f14\u8b1b\u4e2d\uff0c\u4ed6\u5f37\u8abf\u5169\u4f4d\u5bf6\u5bf6\u90fd\u5341\u5206\u300c\u5065\u5eb7\u300d\u3002\u4f46\u679c\u771f\u5982\u6b64\u55ce\uff1f<\/p>\n<p>CRISPR\/Cas9 \u555f\u52d5\u57fa\u56e0\u7de8\u8f2f\u7684\u6548\u7387\u96d6\u9ad8\uff0c\u4f46\u554f\u984c\u4e5f\u4e0d\u5c11\u3002\u76ee\u524d\u6700\u8b93\u79d1\u5b78\u5bb6\u982d\u75db\u7684\u6709\u5169\u5927\u554f\u984c\u3002\u5176\u4e00\uff0c\u662f\u627e\u5230\u554f\u984c\u57fa\u56e0\u7684\u6b63\u78ba\u4f4d\u7f6e\u3002Cas9 \u57fa\u672c\u4e0a\u5c31\u662f\u500b\u8def\u7661\uff0c\u9760\u8457 20 bp \u9577\u5ea6\u7684 crRNA \u6309\u5716\u7d22\u9a65\uff0c\u53bb\u627e\u5230\u8ddf\u90a3 20 bp \u5e8f\u5217\u300c\u543b\u5408\u5ea6\u5920\u9ad8\u300d\u7684\u57fa\u56e0\u7247\u6bb5\u3002\u4f46\u5c31\u50cf\u300c\u6c38\u548c\u6709\u6c38\u548c\u8def\uff0c\u4e2d\u548c\u4e5f\u6709\u6c38\u548c\u8def\uff0c\u4e2d\u548c\u6709\u4e2d\u548c\u8def\uff0c\u6c38\u548c\u4e5f\u6709\u4e2d\u548c\u8def\u300d\u4e00\u6a23\uff0c\u7576 Cas9 \u5728\u6309\u5716\u7d22\u9a65\u3001\u5c0b\u5c0b\u8993\u8993\u7684\u904e\u7a0b\u88e1\uff0c\u96e3\u4fdd\u4e0d\u88ab\u7e5e\u660f\u4e86\u982d\uff0c\u773c\u775b\u4e00\u82b1\u5c31\u8b58 DNA \u4e0d\u660e\u3002\u800c\u4e14\uff0c\u79d1\u5b78\u5bb6\u4e5f\u7684\u78ba\u767c\u73fe\uff0cCas9 \u4e26\u4e0d\u662f\u500b\u5b8c\u7f8e\u504f\u57f7\u72c2\uff0c\u5b83\u4e00\u9ede\u4e5f\u6c92\u6709\u5728\u8ffd\u6c42\u4e0b\u5200\u8655\u7684 DNA \u5e8f\u5217\u5fc5\u9808\u8ddf crRNA \u5e8f\u5217\u767e\u5206\u767e\u543b\u5408\uff0c\u800c\u9019\uff0c\u4e5f\u5c31\u662f\u300c\u812b\u9776\u6548\u61c9\u300d\uff08off-target effect\uff09\u7684\u96b1\u6182\u6e90\u982d\u3002<\/p>\n<p>\u7b2c\u4e8c\u500b\u554f\u984c\uff0c\u662f DNA \u4fee\u88dc\u904e\u7a0b\u7562\u7adf\u9084\u662f\u5f97\u300c\u770b\u5929\u610f\u300d\u3002\u96d6\u7136\uff0c\u5927\u90e8\u5206\u7684\u57fa\u56e0\u7de8\u8f2f\u5be6\u9a57\uff0c\u90fd\u9078\u5728\u53d7\u7cbe\u5375\u9084\u662f\u4e00\u500b\u7d30\u80de\u7684\u6642\u5019\uff0c\u5229\u7528\u986f\u5fae\u6ce8\u5c04\u6cd5\u5c07\u7d44\u88dd\u597d\u7684 Cas9 \u86cb\u767d\u8207\u76f8\u95dc\u300c\u96f6\u4ef6\u300d\uff08\u50cf\u662f crRNA\u3001tracrRNA\uff09\u4ee5\u53ca DNA \u4fee\u5fa9\u6a21\u677f\u6ce8\u5c04\u5230\u53d7\u7cbe\u5375\u5167\u3002\u4f46\u53d7\u7cbe\u5375\u7562\u7adf\u4e5f\u6709\u81ea\u5df1\u4e00\u8b8a\u4e8c\u3001\u4e8c\u8b8a\u56db\u3001\u56db\u8b8a\u516b\u7684\u5206\u88c2\u9032\u5ea6\u3002Cas9 \u86cb\u767d\u662f\u4e0d\u662f\u53ef\u4ee5\u6436\u5728\u7d30\u80de\u5206\u88c2\u958b\u59cb\u524d\uff0c\u5c07 DNA \u96d9\u80a1\u65b7\u88c2\uff0c\u4e26\u8b93\u7d30\u80de\u5167\u7684 DNA \u4fee\u5fa9\u6a5f\u5236\u5b8c\u6210\u5176\u57fa\u56e0\u7de8\u8f2f\u5de5\u4f5c\uff0c\u9084\u771f\u7684\u662f\u500b\u5f97\u770b\u8001\u5929\u723a\u773c\u8272\u7684\u4e8b\u3002CRISPR\/Cas9 \u53ef\u4ee5\u5b8c\u6210\u5de5\u4f5c\uff0c\u4f46\u8981\u4fdd\u8b49\u6240\u51fa\u6210\u54c1\u88e1\uff0c\u6bcf\u4e00\u500b\u7d30\u80de\u90fd\u4e00\u6a21\u4e00\u6a23\uff0c\u5be6\u5728\u4e5f\u662f\u5f37\u5176\u6240\u96e3\u3002\u4e0d\u8ad6\u662f\u5229\u7528\u4eba\u985e\u7d30\u80de\u3001\u6216\u662f\u5c0f\u9f20\u7d30\u80de\uff0c\u90fd\u767c\u73fe\u9019\u6a5f\u7387\u662f\u5fae\u4e4e\u5176\u5fae\u3002\u5927\u90e8\u5206\u7684\u80da\u80ce\u7d30\u80de\u90fd\u5404\u81ea\u9472\u5d4c\u4e86\u8a31\u591a\u4e0d\u540c\u7684\u7a81\u8b8a\uff0c\u9019\u4e5f\u5c31\u662f\u5d4c\u5408\u7a81\u8b8a\uff08mosaic mutations\uff09\u3002\u5728\u9019\u5169\u5927\u554f\u984c\u89e3\u6c7a\u524d\uff0cCRISPR\/Cas9 \u4e4b\u65bc\u907a\u50b3\u6027\u75be\u75c5\uff0c\u5728\u81e8\u5e8a\u61c9\u7528\u4e0a\u9084\u662f\u5f92\u6709\u9858\u666f\u3002<\/p>\n<p>\u8cc0\u5efa\u594e\u627e\u5230\u5c0d\u4ed8 CRISPR\/Cas9 \u9019\u5169\u5927\u554f\u984c\u7684\u65b9\u6cd5\u4e86\u55ce\uff1f\u6c92\u6709\u3002<\/p>\n<p>\u9996\u5148\uff0c\u5728\u4ed6\u6f14\u8b1b\u4e2d\u6240\u516c\u5e03\u7684\u5be6\u9a57\u6578\u64da\u4f86\u770b\uff0c\u6c92\u6709\u4e00\u500b\u5bf6\u5bf6\u7684\u57fa\u56e0\u8b8a\u6210\u50cf <em>CCR5<\/em><em>\u039432<\/em> \u4e00\u6a23\u3002\u4f46\u9019\u4f3c\u4e4e\u4e5f\u662f\u60f3\u5fc5\u5982\u6b64\u7684\u4e8b\u3002\u56e0\u70ba\uff0c\u5728\u986f\u5fae\u6ce8\u5c04\u904e\u7a0b\u4e2d\uff0c\u8cc0\u5efa\u594e\u7684\u7814\u7a76\u5718\u968a\u4e26\u6c92\u6709\u63d0\u4f9b\u57fa\u56e0\u4fee\u5fa9\u7684\u6a21\u677f\uff0c\u8b93\u80da\u80ce\u6642\u671f\u7684\u9732\u9732\u8207\u5a1c\u5a1c\u53d7\u7cbe\u5375\uff0c\u80fd\u5920\u4f9d\u7167\u8457\u6a21\u677f\u4f86\u4f9d\u6a23\u756b\u846b\u8606\u7684\u4fee\u5fa9\u57fa\u56e0\u5e8f\u5217\u6210\u70ba <em>CCR5<\/em><em>\u039432 <\/em>\u7684\u7de8\u78bc\u5e8f\u5217\u3002<\/p>\n<p>\u800c\u9019\u300c\u4e00\u526a\u5200\u4e0b\u53bb\u3001\u4e4b\u5f8c\u5168\u6191\u5929\u610f\u300d\u7684\u7d50\u679c\uff0c\u8b93\u9732\u9732\u548c\u5a1c\u5a1c\u9ad4\u5167\u7d30\u80de\u7684\u57fa\u56e0\u5e8f\u5217\u90fd\u6709\u5d4c\u5408\u7a81\u8b8a\u7684\u554f\u984c\u3002\u300c\u4e00\u822c\u8a8d\u70ba\u300d\uff08\u56e0\u70ba\u5728\u6f14\u8b1b\u4e2d\uff0c\u5bf6\u5bf6\u548c\u76f8\u5c0d\u57fa\u56e0\u5728\u524d\u5f8c\u6295\u5f71\u7247\u7684\u6a19\u793a\u4e26\u4e0d\u7d71\u4e00\uff09\uff0c\u9732\u9732\u9ad4\u5167\u6709 4 bp \u9e7c\u57fa\u5c0d\u7684\u77ed\u5c11\u3001\u8207\u984d\u5916\u591a 1 bp \u5169\u7a2e\u57fa\u56e0\u7de8\u8f2f\u9020\u6210\u7684\u7a81\u8b8a\u3002\u800c\u5a1c\u5a1c\u7684\u60c5\u6cc1\u66f4\u8b93\u4eba\u76ba\u7709\uff0c\u5a1c\u5a1c\u4e0d\u4f46\u9ad4\u5167\u67d0\u4e9b DNA \u9084\u5e36\u6709\u5b8c\u6574\u7684 <em>CCR5<\/em> \u6b63\u5e38\u57fa\u56e0\u5e8f\u5217\uff0c\u53e6\u5916\u6aa2\u9a57\u51fa\u4f86\u7684\u7a81\u8b8a\u53ea\u662f\u77ed\u5c11 15 bp \u9e7c\u57fa\u5c0d\uff0c\u8b93\u5a1c\u5a1c\u7684\u8b8a\u7570 CCR5 \u86cb\u767d\u53ea\u662f\u6bd4\u6b63\u5e38\u86cb\u767d\u5c11\u4e86 5 \u500b\u80fa\u57fa\u9178\uff0c\u4e4b\u5f8c\u5e8f\u5217\u8207\u6b63\u5e38\u86cb\u767d\u7121\u7570\u3002\u800c\u9019\u77ed\u5c11\u7684 5 \u500b\u80fa\u57fa\u9178\u662f\u4e0d\u662f\u8db3\u4ee5\u9020\u6210 CCR5 \u86cb\u767d\u8cea\u7d50\u69cb\u4e0a\u7684\u7834\u58de\uff0c\u4e0d\u4f46\u5c1a\u672a\u53ef\u77e5\uff0c\u5c31\u7b97\u8b8a\u7570 CCR5 \u86cb\u767d\u7684\u8868\u73fe\u7684\u78ba\u53d7\u5230\u5f71\u97ff\uff0c\u90a3\u5176\u4ed6\u6b63\u5e38\u7684 <em>CCR5<\/em> \u57fa\u56e0\u5e8f\u5217\uff0c\u9084\u662f\u53ef\u4ee5\u88fd\u9020\u51fa\u6b63\u5e38\u529f\u80fd\u7684\u86cb\u767d\u8cea\u3002<\/p>\n<p>\u53e6\u5916\uff0c\u5728\u61f7\u5b55\u4e2d\u7684\u57fa\u56e0\u5b9a\u5e8f\uff0c\u6aa2\u9a57\u51fa\u5a1c\u5a1c\u9ad4\u5167 DNA \u53ef\u80fd\u6709\u53e6\u4e00\u6bb5\u5340\u57df\uff0c\u4e5f\u88ab Cas9 \u86cb\u767d\u7de8\u8f2f\u904e\u3002\u96d6\u7136\u6b64\u4e00\u6f5b\u5728\u7684\u812b\u9776\u6548\u61c9\u4e26\u4e0d\u4f4d\u65bc\u5df2\u77e5\u7684\u3001\u6709\u529f\u80fd\u7684\u57fa\u56e0\u8655\uff0c\u4e26\u4e14\u5728\u5a1c\u5a1c\u51fa\u751f\u4e4b\u5f8c\u7684\u9996\u6b21\u57fa\u56e0\u5b9a\u5e8f\u88e1\uff0c\u4e5f\u6c92\u6709\u518d\u6b21\u767c\u73fe\uff0c\u4f46\u9451\u65bc\u76ee\u524d\u57fa\u56e0\u5b9a\u5e8f\u4e0a\u7684\u6280\u8853\u4ecd\u7136\u6709\u5176\u9650\u5236\uff0c\u5c0d\u65bc\u812b\u9776\u6548\u61c9\u7684\u6f5b\u5728\u526f\u4f5c\u7528\uff0c\u662f\u4e0d\u662f\u4e5f\u61c9\u8a72\u66f4\u8b39\u614e\u5c0f\u5fc3\u5462\uff1f<\/p>\n<p>\u5728\u8cc0\u5efa\u594e\u7684\u6f14\u8b1b\u88e1\uff0c\u5c62\u906d\u8cea\u554f\u7684\u4e00\u9ede\u4e5f\u662f\uff0c\u65e2\u7136\u5df2\u77e5\u5a1c\u5a1c\u7684\u57fa\u56e0\u7de8\u8f2f\u4e0d\u751a\u6210\u529f\uff0c\u70ba\u4ec0\u9ebc\u9084\u662f\u57f7\u610f\u8981\u628a\u5a1c\u5a1c\u7684\u80da\u80ce\u690d\u5165\u5462\uff1f\u8cc0\u5efa\u594e\u5c0d\u6b64\u8868\u793a\uff0c\u5718\u968a\u6709\u8ddf\u5a1c\u5a1c\u7684\u6bcd\u89aa\u8a0e\u8ad6\u904e\uff0c\u662f\u5176\u6bcd\u89aa\u6240\u505a\u7684\u6c7a\u5b9a\u3002\u4f46\uff0c\u5c31\u50cf\u5728\u5834\u79d1\u5b78\u5bb6\u7684\u7591\u554f\uff1a\u5982\u679c\u4ec0\u9ebc\u90fd\u63a8\u7d66\u4e86\u5a1c\u5a1c\u6bcd\u89aa\u6240\u505a\u7684\u6c7a\u5b9a\uff0c\u90a3\uff0c\u8eab\u70ba\u4e00\u500b\u79d1\u5b78\u5bb6\u7684\u8cac\u4efb\u5728\u54ea\u88e1\u5462\uff1f<\/p>\n<p>\u9019\u4e5f\u662f\u70ba\u4ec0\u9ebc\u7576\u8cc0\u5efa\u594e\u5728\u7b2c\u4e8c\u5c46\u4eba\u985e\u57fa\u56e0\u7de8\u8f2f\u5b78\u8853\u9ad8\u5cf0\u6703\u4e0a\u7684\u6f14\u8b1b\u4e4b\u5f8c\uff0c\u8b93\u4eba\u66f4\u52a0\u96e3\u904e\uff0c\u4e5f\u7121\u52a9\u65bc\u5e73\u606f\u8f3f\u8ad6\u7684\u539f\u56e0\u4e4b\u4e00\u3002\u800c\u4e14\uff0c\u81ea\u5f9e\u65b0\u805e\u7206\u767c\u4e4b\u5f8c\uff0c\u548c\u9019\u8d77\u81e8\u5e8a\u8a66\u9a57\u76f8\u95dc\u7684\u502b\u7406\u5be9\u67e5\u8ca0\u9762\u6d88\u606f\u5c31\u4e0d\u66fe\u505c\u904e\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u8a31\u591a\u8072\u97f3\u4e5f\u90fd\u8981\u6c42\u8cc0\u5efa\u594e\u61c9\u8a72\u628a\u5be6\u9a57\u7d50\u679c\u6295\u7a3f\u5230\u671f\u520a\uff0c\u4ee5\u8b93\u540c\u9818\u57df\u5c08\u5bb6\u80fd\u5920\u5c0d\u5be6\u9a57\u6578\u64da\u52a0\u4ee5\u5be9\u67e5\uff08\u4f46\u51fa\u5305\u7684\u81e8\u5e8a\u8a66\u9a57\u502b\u7406\u5be9\u67e5\u4e5f\u6703\u662f\u4e00\u5927\u963b\u7919\uff1b\u4f46\u8cc0\u5efa\u594e\u8868\u793a\u5df2\u7d93\u6295\u7a3f\uff09\uff0c\u6216\u662f\uff0c\u662f\u4e0d\u662f\u61c9\u8a72\u5c07\u9810\u5370\u672c\uff08preprint\uff09\u653e\u5230\u50cf\u662f Biorxiv \u7684\u8cc7\u6599\u5eab\u5e73\u53f0\u4e0a\u3002\u4ee5\u8afe\u8c9d\u723e\u5f97\u4e3b David Baltimore \u70ba\u9996\u7684\u8072\u97f3\u4e5f\u547c\u7c72\uff0c\u61c9\u8a72\u8981\u8b93\u53e6\u4e00\u500b\u7368\u7acb\u7684\u7b2c\u4e09\u65b9\u6a5f\u69cb\uff0c\u6709\u6a5f\u6703\u53ef\u4ee5\u6aa2\u67e5\u9732\u9732\u8207\u5a1c\u5a1c\u7684\u76f8\u95dc\u7814\u7a76\u6578\u64da\u8207\u5065\u5eb7\u60c5\u6cc1\uff0c\u78ba\u8a8d\u5c0f\u5b69\u5065\u5eb7\u7121\u865e\u3001\u4ee5\u53ca\u76f8\u95dc\u6aa2\u9a57\u7d50\u679c\u6c92\u6709\u932f\u8aa4\u3002<\/p>\n<p>\u8cc0\u5efa\u594e\u70ba\u4ec0\u9ebc\u770b\u4e0a\u4e86 <em>CCR5<\/em> \u9019\u500b\u57fa\u56e0\uff1f\u9732\u9732\u548c\u5a1c\u5a1c\u9ad4\u5167\u88ab\u7de8\u8f2f\u904e\u7684\u57fa\u56e0\uff0c\u771f\u80fd\u5920\u50cf <em>CCR5<\/em><em>\u039432<\/em> \u4e00\u6a23\u63d0\u4f9b\u5979\u5011\u4e9b\u8a31\u514d\u65bc\u5f8c\u5929\u514d\u75ab\u4e0d\u5168\u75c7\u7684\u4fdd\u8b77\u55ce\uff1f\uff08\u5c0d\u5a1c\u5a1c\u4f86\u8aaa\uff0c\u61c9\u8a72\u662f\u78ba\u5b9a\u4e0d\u884c\uff09\u3002\u6240\u8b02\u4e8b\u7121\u5341\u5168\uff0c\u5c0d\u65bc\u6bd4\u8f03\u80fd\u5920\u514d\u75ab\u65bc HIV \u75c5\u6bd2\u611f\u67d3\u7684\u500b\u9ad4\uff0c\u96e3\u9053\u5c31\u6c92\u6709\u5176\u4ed6\u5065\u5eb7\u4e0a\u7684\u4ee3\u50f9\u55ce\uff1f\u8b93\u6211\u5011\u5728\u4e0b\u4e00\u7bc7\u4e00\u8d77\u4f86\u4ed4\u7d30\u770b\u770b\u5427\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u539f\u59cb\u5831\u5c0e\uff1a&nbsp;<\/strong><\/p>\n<p>\u8cc0\u5efa\u594e\u672c\u4f86\u5148\u5c07\u57fa\u56e0\u7de8\u8f2f\u5bf6\u5bf6\u7684\u6d88\u606f\u900f\u9732\u7d66 Associated Press \u8a18\u8005\uff1aMarilynn Marchione. <a href=\"https:\/\/www.apnews.com\/4997bb7aa36c45449b488e19ac83e86d\">Chinese researcher claims first gene-edited babies.<\/a> Associated Press. Nov 26, 2018.&nbsp;<\/p>\n<p>&nbsp;\u5f8c\u4f86\uff0cMIT Technology Review \u8ca0\u8cac\u751f\u91ab\u9818\u57df\u7684\u7de8\u8f2f\uff0c\u8033\u805e\u4e86\u8cc0\u5efa\u9035\u7684\u7814\u7a76\u7d50\u679c\u800c\u6436\u5148\u4e00\u6b65\u5c07\u57fa\u56e0\u7de8\u8f2f\u5bf6\u5bf6\u7684\u65b0\u805e\u62ab\u9732\uff1aAntonio Regalado. EXCLUSIVE: <a href=\"https:\/\/www.technologyreview.com\/s\/612458\/exclusive-chinese-scientists-are-creating-crispr-babies\/\">Chinese scientists are creating CRISPR babies. <\/a>MIT Technology Review. Nov 25, 2018.&nbsp;<\/p>\n<p>\u8cc0\u5efa\u594e\u7684<a href=\"https:\/\/www.youtube.com\/watch?v=cH57-YO9Eso&amp;feature=youtu.be&amp;fbclid=IwAR0gdtNZTl-TDkgolVcoot58AI5VD-gkUcvgAbULMEU7RbAR4mnCRq4jgqs\">\u6f14\u8b1b\u9304\u5f71\u8207\u6f14\u8b1b\u5f8c\u8a0e\u8ad6<\/a>\uff08\u5f9e1:06:59 \u5de6\u53f3\u958b\u59cb\uff0c\u7d04\u4e8c\u5341\u5206\u9418\u7684\u6f14\u8b1b\uff0c\u8207\u56db\u5341\u5206\u9418\u7684\u8a0e\u8ad6\uff09<\/p>\n<p>\u8cc0\u5efa\u594e\u7684<a href=\"https:\/\/drive.google.com\/drive\/folders\/1T1zLTtHS2z_cgl29fN_7qJg7fLA4qlrd\">\u6f14\u8b1b\u6295\u5f71\u7247<\/a>&nbsp;<\/p>\n<p>\u8cc0\u5efa\u594e\u7684<a href=\"http:\/\/diyhpl.us\/wiki\/transcripts\/human-genome-editing-summit\/2018-hong-kong\/jiankui-he-human-genome-editing\/\">\u6f14\u8b1b\u9010\u5b57\u7a3f<\/a>\uff1a\uff08\u7531 Bryan Bishop \u6240\u6574\u7406\uff09<\/p>\n<p><strong>\u53c3\u8003\u8cc7\u6599\uff1a&nbsp;<\/strong><\/p>\n<ol>\n<li>Dennis Normile. Researcher who created CRISPR twins defends his work but leaves many questions unanswered. Science. Nov 28, 2018. <a href=\"https:\/\/www.sciencemag.org\/news\/2018\/11\/researcher-who-created-crispr-twins-defends-his-work-leaves-many-questions-unanswered\">https:\/\/www.sciencemag.org\/news\/2018\/11\/researcher-who-created-crispr-twins-defends-his-work-leaves-many-questions-unanswered<\/a><\/li>\n<li>Jon Cohen. Ethics aside, does the CRISPR baby experiment make scientific sense? Science. Nov 28, 2018. <a href=\"https:\/\/www.sciencemag.org\/news\/2018\/11\/ethics-aside-does-crispr-baby-experiment-make-scientific-sense\">https:\/\/www.sciencemag.org\/news\/2018\/11\/ethics-aside-does-crispr-baby-experiment-make-scientific-sense<\/a><\/li>\n<li>Derek Lowe. After Such Knowledge. Nov 28, 2018. <a href=\"https:\/\/blogs.sciencemag.org\/pipeline\/archives\/2018\/11\/28\/after-such-knowledge?fbclid=IwAR18RYtJhfItRKeNgte0zSgOwY9T74OdbmBeVjLpYsLlyhQcyRP_NQg2lOU\">https:\/\/blogs.sciencemag.org\/pipeline\/archives\/2018\/11\/28\/after-such-knowledge?fbclid=IwAR18RYtJhfItRKeNgte0zSgOwY9T74OdbmBeVjLpYsLlyhQcyRP_NQg2lOU<\/a><\/li>\n<li>David Cyranoski. CRISPR-baby scientist fails to satisfy critics. <a href=\"https:\/\/www.nature.com\/articles\/d41586-018-07573-w\">https:\/\/www.nature.com\/articles\/d41586-018-07573-w<\/a><\/li>\n<li>Sharon Begley. Amid uproar, Chinese scientist defends creating gene-edited babies. STAT. Nov 28, 2018. https:\/\/www.statnews.com\/2018\/11\/28\/chinese-scientist-defends-creating-gene-edited-babies\/<\/li>\n<li>Akshat Rathi. The Crispr baby news was carefully orchestrated PR\u2014until it all went wrong. https:\/\/qz.com\/1474814\/the-cripsr-baby-news-was-carefully-orchestrated-pr-until-it-all-went-wrong\/<\/li>\n<li>\u8cfd\u5148\u751f. 300 \u9918\u4f4d\u5b78\u8005\u806f\u540d\uff1a\u5341\u554f\u8cc0\u5efa\u594e. Nov 30, 2018. <a href=\"http:\/\/m.zhishifenzi.com\/depth\/depth\/4711.html?from=singlemessage&amp;isappinstalled=0&amp;fbclid=IwAR0fSEmg40viRVYdMjjy3QqV_q6A4mDWbTRBTT8u1bIFycbz8bR-TzgkKn0\">http:\/\/m.zhishifenzi.com\/depth\/depth\/4711.html?from=singlemessage&amp;isappinstalled=0&amp;fbclid=IwAR0fSEmg40viRVYdMjjy3QqV_q6A4mDWbTRBTT8u1bIFycbz8bR-TzgkKn0<\/a><\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p><strong>--<\/strong><br \/>\n<strong>\u4f5c\u8005\uff1a\u99f1\u5b9b\u7433\u3000\u7f8e\u570b\u8056\u8def\u6613\u83ef\u76db\u9813\u5927\u5b78\uff08Washington University in St. Louis\uff09\u514d\u75ab\u5b78\u535a\u58eb\uff0c\u5f9e\u4e8bT\u7d30\u80de\u767c\u80b2\u8207\u6d3b\u5316\u76f8\u95dc\u7684\u8a0a\u606f\u50b3\u5c0e\u7814\u7a76\u3002<\/strong><\/p>\n<p><a href=\"https:\/\/line.me\/R\/ti\/p\/%40fcx6182w\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/scdn.line-apps.com\/n\/line_add_friends\/btn\/zh-Hant.png\" alt=\"\u52a0\u5165\u597d\u53cb\" width=\"212\" height=\"36\" border=\"0\"><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u25a0\u8cc0\u5efa\u594e\uff0c\u5e74\u4e09\u5341\u56db\uff0c\u7559\u8077\u505c\u85aa\u4e2d\u570b\u5357\u65b9\u79d1\u6280\u5927\u5b78\u526f\u6559\u6388\uff0c\u5728 2018 \u5e74\u5341\u4e00\u6708\u7684\u6700\u5f8c\u4e00\u500b\u9031\u672b\uff0c\u9707\u64bc\u4e86\u5168\u7403\u793e\u6703\u8207\u79d1\u5b78\u754c\u3002\u8cc0\u5148\u751f\u505a\u4e86\u4ec0\u9ebc\uff1f\u4ed6\u5229\u7528 CRISPR\/Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u7ac4\u5beb\u4e86\u4eba\u985e\u80da\u80ce\u57fa\u56e0\u9ad4\u5e8f\u5217\u3002\u4ed6\u4e0d\u662f\u70ba\u4e86\u6cbb\u7642\u7f55\u898b\u7684\u3001\u8b93\u4eba\u675f\u624b\u7121\u7b56\u7684\u907a\u50b3\u6027\u75be\u75c5\uff0c\u4ed6\u8aaa\u4ed6\u60f3\u8981\u8b93\u4eba\u985e\u514d\u65bc HIV \u75c5\u6bd2\u3002\u4ed6\u89ba\u5f97\u9732\u9732\u548c\u5a1c\u5a1c\u7684\u7238\u7238\u662f HIV \u5e36\u539f\u8005\u5f88\u53ef\u6190\uff0c\u60f3\u8981\u7d66\u53d7\u632b\u65bc\u985e\u4f3c\u60c5\u6cc1\u7684\u7238\u7238\u5011\u4e00\u500b\u5e0c\u671b\u3002\u65bc\u662f\uff0c\u4ed6\u9818\u982d\u4e86\u4e00\u7d44\u81e8\u5e8a\u5be6\u9a57\u8a08\u756b\uff0c\u610f\u5728\u5229\u7528\u57fa\u56e0\u7de8\u8f2f\u6280\u8853\uff0c\u8b93\u9019\u4e9b\u5bb6\u5ead\u7684\u5f8c\u4ee3\uff0c\u80fd\u5920\u6709\u500b\u300c\u514d\u75ab\u300d\u65bc HIV \u75c5\u6bd2\u611f\u67d3\u7684\u5b69\u5b50\u3002<\/p>\n","protected":false},"author":20,"featured_media":33337,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,1025],"tags":[3075,256,4115,4114],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2018\/12\/47085995_2256292061049744_1519136843197579264_o.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/33336"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33336"}],"version-history":[{"count":7,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/33336\/revisions"}],"predecessor-version":[{"id":33389,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/33336\/revisions\/33389"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/33337"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33336"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33336"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33336"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}