{"id":30862,"date":"2018-05-29T00:30:39","date_gmt":"2018-05-28T16:30:39","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=30862"},"modified":"2018-07-29T19:31:21","modified_gmt":"2018-07-29T11:31:21","slug":"%e3%80%90%e6%8e%a2%e7%b4%a219-3%e3%80%91%e5%be%9e%e5%9f%ba%e5%9b%a0%e4%b9%8b%e5%b0%8f-%e8%a6%8b%e7%94%9f%e5%91%bd%e4%b9%8b%e5%a4%a7%ef%bc%9a%e8%a7%a3%e7%a2%bcdna%e8%b6%b3%e8%b7%a1","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=30862","title":{"rendered":"\u3010\u63a2\u7d2219-3\u3011\u5f9e\u57fa\u56e0\u4e4b\u5c0f \u898b\u751f\u547d\u4e4b\u5927\uff1a\u89e3\u78bcDNA\u8db3\u8de1"},"content":{"rendered":"<p><span style=\"color: #333399;\"><strong>\u25a0\u672c\u671f\u63a2\u7d22\u8b1b\u5ea7\u7b2c\u4e09\u8b1b\u7531\u4e8e\u5b8f\u71e6\u6559\u6388\u64d4\u4efb\u4e3b\u8b1b\u4eba\uff0c\u4e8e\u8001\u5e2b\u7814\u7a76\u9818\u57df\u6a6b\u8de8\u6f14\u5316\u907a\u50b3\u8207\u57fa\u56e0\u9ad4\u5b9a\u5e8f\uff0c\u672c\u6b21\u6f14\u8b1b\u9664\u4e86\u4ee5\u5de7\u5999\u7684\u6bd4\u55bb\u53ca\u751f\u52d5\u53e3\u543b\u5c07\u57fa\u56e0\u5b9a\u5e8f\u767c\u5c55\u8108\u7d61\u5a13\u5a13\u9053\u4f86\u5916\uff0c\u4e5f\u5f15\u9818\u89c0\u773e\u601d\u8003\u73fe\u4eca\u4e16\u754c\u9762\u81e8\u4e4b\u96e3\u984c\u53ca\u904b\u7528\u57fa\u56e0\u9ad4\u5e8f\u5217\u7684\u53ef\u80fd\u56e0\u61c9\u4e4b\u9053\u3002<\/strong><\/span><\/p>\n<figure id=\"attachment_30969\" aria-describedby=\"caption-attachment-30969\" style=\"width: 450px\" class=\"wp-caption alignleft\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-30969\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2018\/05\/Flickr-@microphylum.jpg\" alt=\"\" width=\"450\" height=\"312\" \/><figcaption id=\"caption-attachment-30969\" class=\"wp-caption-text\">microphylum@Flickr<\/figcaption><\/figure>\n<p><strong>\u8b1b\u5e2b\uff5c\u81fa\u7063\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u6559\u6388 \u4e8e\u5b8f\u71e6<br \/>\n<\/strong><strong> \u5f59\u6574\u64b0\u6587\uff5c\u9673\u96c5\u5a77<\/strong><\/p>\n<p><span style=\"color: #008080;\"><strong>\u25cf\u57fa\u56e0\u5167\u7684\u7d50\u7e69\u7d00\u4e8b<\/strong><\/span><\/p>\n<p>\u96fb\u8166\u4ee50\u82071\u7684\u6392\u5217\u65b9\u5f0f\u4f86\u5132\u5b58\u8cc7\u8a0a\uff0c\u751f\u7269\u5247\u662f\u7531\u6700\u57fa\u672c\u5b57\u5143A\u3001T\u3001C\u3001G\u56db\u7a2e\u9e7c\u57fa\u8a18\u9304\u4e14\u6c7a\u5b9a\u907a\u50b3\u7279\u5fb5\uff0c\u900f\u904e\u6392\u5217\u7d44\u5408\u7684\u8b8a\u5316\uff0c\u5f62\u6210\u5404\u5f0f\u6307\u4ee4\u9032\u800c\u5408\u6210\u86cb\u767d\u8cea\u8abf\u63a7\u5404\u7a2e\u7d30\u80de\u548c\u751f\u7269\u6a5f\u80fd\u3002\u800c\u9019\u6a23\u7684\u6a5f\u5236\u7a76\u7adf\u6709\u591a\u7cbe\u5bc6?\u6211\u5011\u53ef\u4ee5\u8a66\u8457\u9019\u9ebc\u60f3\uff1a\u5982\u679c\u5c07\u8eab\u4e0a46\u689d\u67d3\u8272\u9ad4\u7684DNA\u5f9e\u7d30\u80de\u4e2d\u62bd\u51fa\uff0c\u4e26\u5c07\u5b83\u5011\u6392\u5217\u8d77\u4f86\uff0c\u8a08\u7b97\u5f8c\u53ef\u90542-3\u516c\u5c3a\u9577\uff0c\u800c\u9019\u4e9b\u300c\u751f\u547d\u85cd\u5716\u300d\u7684\u9f90\u5927\u5bc6\u78bc\u8cc7\u8a0a\u5fc5\u9808\u7dca\u5bc6\u7e8f\u7e5e\u58d3\u7e2e\u81f3\u5728\u5e7e\u500b\u5fae\u7c73\u7684\u7d30\u80de\u6838\u5167\uff1b\u5982\u679c\u6211\u5011\u518d\u9032\u4e00\u6b65\u5c07\u5168\u8eab\u7d30\u80de\u7684DNA\u62c9\u76f4\u8a08\u7b97\uff0c\u90a3\u53ef\u662f\u76f8\u7576\u65bc\u5730\u7403\u5230\u592a\u967d\u4f86\u56de\u7d0470\u8d9f\u7684\u9577\u5ea6\u3002\u4f46\u5e7e\u767e\u842c\u5e74\u4f86\uff0c\u751f\u7269\u537b\u5f97\u4ee5\u900f\u904e\u9019\u4e9b\u9f90\u5927\u4e14\u7cbe\u78ba\u7684\u5bc6\u78bc\u7d00\u4e8b\uff0c\u5c07\u7279\u5fb5\u4ee3\u4ee3\u76f8\u50b3\uff0c\u4e26\u96a8\u8457\u6642\u9593\u6f14\u5316\uff0c\u9020\u5c31\u51fa\u5730\u7403\u751f\u7269\u5708\u7684\u591a\u8b8a\u6a23\u8c8c\u3002<!--more--><\/p>\n<p>\u503c\u5f97\u4e00\u63d0\u7684\u662f\uff0c\u5982\u679c\u6211\u5011\u9032\u4e00\u6b65\u89c0\u5bdf\u5404\u7269\u7a2e\u7684\u67d3\u8272\u9ad4\u6578\u76ee\u6216DNA\u542b\u91cf\u8b8a\u5316\uff0c\u79d1\u5b78\u5bb6\u767c\u73fe\u5b58\u5728\u8457\u300cC\u503c\u77db\u76fe\u73fe\u8c61\uff08C \u2013 value paradox\uff09\u300d\u3002C\u503c\u6307\u7684\u662f\u4ec0\u9ebc\u5462\uff1f\u5176\u5be6\u5c31\u662f\u771f\u6838\u751f\u7269\u7d30\u80de\u4e2d\uff0c\u55ae\u500d\u9ad4\u7d30\u80de\u6838\u88e1\u6240\u64c1\u6709\u7684DNA\u542b\u91cf\u3002\u79d1\u5b78\u5bb6\u767c\u73fe\u751f\u7269\u7684C\u503c\uff08\u5373\u57fa\u56e0\u7d44DNA\u7684\u91cf\uff09\u5176\u5be6\u4e26\u4e0d\u5168\u7136\u8207\u751f\u7269\u8907\u96dc\u5ea6\u5448\u5b8c\u5168\u6b63\u76f8\u95dc\u3002\u4f8b\u5982\uff1a\u8568\u985e\u4e2d\u540d\u70ba\u74f6\u723e\u5c0f\u8349\u7684\u690d\u7269\u67d3\u8272\u9ad4\u6578\u76ee\u662f2n&gt;1,600\uff0c\u5c31\u9060\u8d85\u904e\u4eba\u985e\u768446\u689d\u67d3\u8272\u9ad4\u3002\u9019\u4e9b\u73fe\u8c61\u9664\u4e86\u7d66\u79d1\u5b78\u5bb6\u51fa\u4e86\u4e00\u9053\u6709\u8da3\u7684\u96e3\u984c\u5916\uff0c\u5c31\u53e6\u4e00\u5c64\u9762\u800c\u8a00\uff0c\u4e5f\u986f\u793a\u4e86\u751f\u7269\u5929\u66f8\u6240\u8a18\u8f09\u7684\u6307\u4ee4\uff0c\u5176\u8b8a\u5316\u548c\u8907\u96dc\u5ea6\u9060\u8d85\u904e\u6211\u5011\u7684\u60f3\u50cf\u3002<\/p>\n<p><span style=\"color: #008080;\"><strong>\u25cf\u91d1\u9470\u958b\u555f\u7bc7\u7ae0<\/strong><\/span><\/p>\n<p>\u96d6\u7136\u5df2\u77e5DNA\u8a18\u4e8b\u662f\u63a1\u7528A\u3001T\u3001C\u3001G\u56db\u7a2e\u57fa\u672c\u5b57\u5143\uff0c\u4f46\u57fa\u56e0\u5e8f\u5217\u53c8\u7576\u5982\u4f55\u6392\u5e8f\uff1f\u5982\u679c\u4ee5\u5be6\u969b\u6587\u5b57\u8207\u66f8\u672c\u4f5c\u70ba\u5f62\u5bb9\uff0c\u4e00\u500b\u4eba\u985e\u57fa\u56e0\u7d44\u7684DNA\u5bc6\u78bc\u5b57\u6578\uff0c\u7d04\u753130\u5104\u500b\u6587\u5b57\u7d44\u6210\uff0c\u76f8\u7576\u65bc75\u5957\u5927\u82f1\u767e\u79d1\u5168\u66f8\uff0c\u8981\u5c07\u5176\u6b63\u78ba\u7834\u89e3\uff0c\u5be6\u975e\u6613\u4e8b\uff0c\u5438\u5f15\u5927\u91cf\u79d1\u5b78\u5bb6\u7d5e\u76e1\u8166\u6c41\u6295\u5165\u548c\u5617\u8a66\u3002\u5176\u4e2d\uff0c\u6700\u5927\u7684\u8e8d\u9032\u5c31\u5c6c1977 \u5e74\u82f1\u570b\u528d\u6a4b\u5927\u5b78Frederick Sanger\u5718\u968a\u6240\u4f7f\u7528\u7684\u805a\u5408\u93c8\u7d42\u6b62\u6cd5\uff08chain- termination method\uff09\uff0c\u5f8c\u4f86\u4ea6\u901a\u7a31\u6851\u683c\u5b9a\u5e8f\u6cd5\u3002<\/p>\n<p>Sanger \u767c\u660e\u7684\u5b9a\u5e8f\u539f\u7406\uff0c\u5176\u7cbe\u9ad3\u5c31\u5728\u65bc\u5229\u7528DNA\u5408\u6210\u6642\u805a\u5408\u9176\u53cd\u61c9\u7684\u7279\u6027\uff0c\u4e26\u4f9d\u6b64\u8a2d\u8a08\u53cd\u61c9\u8a66\u5291\u3002\u4f9d 4 \u7a2e\u9e7c\u57fa\u5206\u985e\uff0c\u9664\u4e86\u52a0\u5165\u4e00\u822cDNA\u5408\u6210\u6240\u9700\u7684\u53bb\u6c27\u6838\u82f7\u9178\uff08dNTPs\uff09\u5916\uff0c\u4e5f\u52a0\u5165\u4e00\u5b9a\u6bd4\u4f8b\u4ee5\u653e\u5c04\u6027\u540c\u4f4d\u7d20\u6a19\u8a18\u7684\u96d9\u53bb\u6c27\u6838\u82f7\u9178\uff08ddNTPs\uff09\u3002\u7531\u65bcddNTPs\u6bd4\u6b63\u5e38\u7684 dNTPs \u5728\u4e09\u865f\u78b3\u4e0a\u518d\u5c11\u4e86\u4e00\u500bOH\u57fa\uff0c\u82e5 DNA \u5408\u6210\u6642\u63a5\u4e0a\u5c11\u4e86OH\u57fa\u7684 ddNTPs\uff0c\u5c31\u6703\u7121\u6cd5\u7e7c\u7e8c\u9032\u884c\u53cd\u61c9\u800c\u7d42\u6b62\u3002\u7531\u65bc\u63a5\u4e0addNTPs\u662f\u96a8\u6a5f\u7684\uff0c\u4e5f\u5c31\u53ef\u4ee5\u5f97\u5230\u5404\u7a2e\u7d42\u6b62\u65bc\u4e0d\u540c\u9577\u5ea6\u7684DNA \u7247\u6bb5\uff0c\u6700\u5f8c\u518d\u900f\u904e\u81a0\u9ad4\u96fb\u6cf3\u5206\u6790\u548c\u986f\u5f71\uff0c\u5373\u53ef\u4f9d\u64da\u96fb\u6cf3\u4f4d\u7f6e\u5716\u8b80\u51faDNA \u5e8f\u5217\u3002<\/p>\n<p>\u540c\u5e74\uff0cSanger\u5718\u968a\u4e5f\u9760\u6b64\u539f\u7406\uff0c\u7834\u89e3\u4e86\u7b2c\u4e00\u672c\u8ff7\u4f60\u672c\u5929\u66f8\uff0c\u4e5f\u5c31\u662f\u300c\u566c\u83cc\u9ad4\u03a6X174\u300d\u7684\u57fa\u56e0\u5e8f\u5217\uff0c\u7e3d\u95775,386bps\u3002\u9019\u9805\u6280\u8853\u7b49\u65bc\u70ba\u4eba\u985e\u8b80\u53d6\u548c\u7406\u89e3\u57fa\u56e0\u5e8f\u5217\u5960\u5b9a\u4e86\u539a\u5be6\u57fa\u790e\uff0cSanger\u672c\u4eba\u4e5f\u7e7c1958\u5e74\u5f8c\u518d\u5ea6\u7372\u5f971980\u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u6b8a\u69ae<strong><sup>[1]<\/sup><\/strong>\u3002\u723e\u5f8c\uff0c\u5728Sanger\u5b9a\u5e8f\u6cd5\u7684\u57fa\u790e\u4e0a\uff0c80 \u5e74\u4ee3\u51fa\u73fe\u4ee5\u87a2\u5149\u6a19\u8a18\u4ee3\u66ff\u653e\u5c04\u6027\u540c\u4f4d\u7d20\u548c\u81ea\u52d5\u5b9a\u5e8f\u5100\u5668\uff0c90 \u5e74\u4ee3\u66f4\u767c\u5c55\u51fa\u6bdb\u7d30\u7ba1\u96fb\u6cf3\u6280\u8853\uff0c\u4f7f\u5f97\u55ae\u4f4d\u6642\u9593\u5167\u7684\u5b9a\u5e8f\u80fd\u529b\u5927\u5e45\u63d0\u5347\u3002<\/p>\n<p>\u6b64\u5916\uff0c\u805a\u5408\u9175\u7d20\u93c8\u9396\u53cd\u61c9\uff08PCR\uff09\u7684\u767c\u660e\uff0c\u66f4\u662f\u53e6\u4e00\u500b\u52a0\u901f\u8fd1\u4ee3\u57fa\u56e0\u5de5\u7a0b\u767c\u5c55\u7684\u5e55\u5f8c\u529f\u81e3\u3002\u96d6\u7136\u9019\u9805\u6280\u8853\u4e5f\u53ea\u4e0d\u904e\u65bc1983\u5e74\u7531Kary B. Mullis\u63d0\u51fa\uff0c\u4f46\u5df2\u6210\u70ba\u5206\u6790DNA\u4e0d\u53ef\u6216\u7f3a\u7684\u6280\u8853\u3002\u6709\u4e86\u9019\u500b\u65b9\u6cd5\uff0c\u6211\u5011\u80fd\u5728\u5e7e\u500b\u5c0f\u6642\u5167\uff0c\u5c07\u7279\u5b9aDNA\u7247\u6bb5\u5728\u8a66\u7ba1\u5167\u8907\u88fd\u4e0a\u767e\u842c\u6b21\uff0c\u4e0d\u8ad6\u5c0d\u65bc\u91ab\u7642\u6aa2\u6e2c\u548c\u73fe\u5834\u5211\u4e8b\u9451\u5b9a\u4e0a\uff0c\u90fd\u63d0\u4f9b\u4e86\u4e00\u4e9b\u65b0\u7684\u53ef\u80fd\uff0c\u751a\u81f3\u662f\u5df2\u7d55\u8de1\u7684\u7269\u7a2e\u6216\u53e4\u8001\u4eba\u985e\u57fa\u56e0\uff08ancient human DNA\uff09\u7814\u7a76\u4e5f\u6709\u4e86\u7a81\u7834\u3002\u7576\u7136\uff0cMullis \u4e5f\u56e0\u6b64\u6210\u5c31\u7372\u5f971993\u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\uff0c\u4ee5\u8868\u5f70\u9019\u9805\u6380\u8d77\u9769\u547d\u6027\u767c\u5c55\u7684\u767c\u660e\u3002<\/p>\n<p><span style=\"color: #008080;\"><strong>\u25cf\u6f14\u7e79\u7d93\u5178 \u767e\u5bb6\u722d\u9cf4<\/strong><\/span><\/p>\n<p>\u986f\u7136\u79d1\u5b78\u5bb6\u5728\u64c1\u6709\u8db3\u4ee5\u89e3\u8b80\u5e8f\u5217\u6280\u8853\u7684\u52a0\u6301\u4e0b\uff0c\u5176\u91ce\u5fc3\u548c\u7b97\u76e4\u52e2\u5fc5\u52d5\u5230\u4eba\u985e\u982d\u4e0a\uff0c\u671f\u671b\u80fd\u6b63\u78ba\u63cf\u8ff0\u4e26\u89e3\u7b54\u5404\u7ae0\u7bc0\u6307\u4ee4\u7684\u4f86\u9f8d\u53bb\u8108\u3002\u4eba\u985e\u57fa\u56e0\u9ad4\u8a08\u756b\uff08Human Genome Project, HGP\uff09\u59cb\u65bc\u4e5d\u25cb\u5e74\u4ee3\u521d\u671f\uff0c\u8d77\u521d\u7531\u79d1\u5b78\u5bb6Renato Dulbecco\u65bc 1985\u5e74\u63d0\u51fa\uff0c1990\u5e74\u7f8e\u570b\u5b98\u65b9\u62cd\u677f\u5b9a\u6848\u8d0a\u52a9\u70ba\u671f15\u5e7430\u5104\u7f8e\u91d1\u8a08\u756b\uff0c\u4e26\u900f\u904e\u570b\u969b\u5be6\u9a57\u5ba4\u5408\u4f5c\uff0c\u5305\u62ec\u7f8e\u3001\u82f1\u3001\u65e5\u3001\u6cd5\u3001\u5fb7\u3001\u897f\u73ed\u7259\u3001\u4e2d\u570b\u3001\u6fb3\u6d32\u7b49\u8de8\u570b\u5408\u4f5c\u3002\u4e0d\u904e\uff0c\u8207\u6b64\u540c\u6642\uff0c\u7f8e\u570b\u79c1\u4eba\u4f01\u696dCelera\u4e5f\u4e0d\u7518\u793a\u5f31\uff0c\u96c6\u8cc73\u5104\u7f8e\u91d1\u6295\u5165\u7814\u7a76\uff0c\u63a1\u53d6\u53e6\u985e\u7684\u7b56\u7565\uff0c\u6700\u5f8c\u751a\u81f3\u6253\u6557\u653f\u5e9c\u901f\u5ea6\u3002<\/p>\n<p>\u5f9e\u5b98\u65b9\u8207\u6c11\u9593\u7d44\u7e54\u7684\u7af6\u722d\u5230\u5f8c\u4f86\u651c\u624b\u5408\u4f5c\uff0c\u90fd\u8db3\u4ee5\u770b\u51fa\u4eba\u985e\u57fa\u56e0\u5e8f\u5217\u7684\u91cd\u8981\u6027\u548c\u773e\u6240\u671f\u5f85\uff0c\u4f46\u5982\u679c\u6709\u5ee0\u5546\u6253\u7684\u662f\u57fa\u56e0\u5c08\u5229\u7684\u4e3b\u610f\uff0c\u90a3\u53ef\u5c31\u7b97\u932f\u4e86\u30022000\u5e74\u7f8e\u570b\u7e3d\u7d71\u67ef\u6797\u9813\u5ba3\u5e03\u4eba\u985e\u57fa\u56e0\u9ad4\u8cc7\u8a0a\u5c07\u5b8c\u5168\u514d\u8cbb\u516c\u958b\u65bc\u7f8e\u570b\u570b\u5bb6\u751f\u7269\u8cc7\u8a0a\u4e2d\u5fc3\uff08NCBI\uff09\u8cc7\u6599\u5eab\uff0c\u78ba\u7acb\u5929\u7136\u57fa\u56e0\u5e8f\u5217\u6b78\u5c6c\u65bc\u81ea\u7136\u7522\u7269\uff0c\u4e0d\u53ef\u7533\u8acb\u5c08\u5229\u7684\u539f\u5247\uff08\u4f46cDNA\u548c\u7d93\u4eba\u70ba\u7de8\u8b6f\u8005\u53ef\u4ee5\uff09\u3002\u6b64\u8209\u4e0d\u4f46\u5efa\u7acb\u5283\u6642\u4ee3\u7684\u91cc\u7a0b\u7891\uff0c\u4e5f\u70ba2003\u5e74\u6b63\u5f0f\u5b8c\u6210\u7684\u4eba\u985e\u57fa\u56e0\u9ad4\u8a08\u756b\uff0c\u63ed\u958b\u4e0b\u4e00\u500b\u5e8f\u5e55\u3002<\/p>\n<p>\u81f3\u6b64\uff0c\u4eba\u985e\u57fa\u56e0\u9ad4\u8a08\u756b\u7684\u5b8c\u6210\uff0c\u8b93\u6211\u5011\u5f9e\u904e\u53bb\u4e00\u7121\u6240\u77e5\uff0c\u5230\u80fd\u8b80\u51fa\u7de8\u6392\u6709\u5e8f\u7684\u300c\u6709\u5b57\u5929\u66f8\u300d\u3002\u4f46\u79d1\u5b78\u5bb6\u53ef\u4e0d\u56e0\u6b64\u6eff\u8db3\uff0c\u540c\u5e749\u6708\u5373\u5c55\u958b\u300cENCODE\u8a08\u5283\u300d \uff08ENCODE=The Encyclopedia of DNA Elements\uff09\uff0c\u76ee\u6a19\u662f\u8a3b\u89e3\u4eba\u985e\u57fa\u56e0\u9ad4\u7684\u6240\u6709\u529f\u80fd\uff0c\u56e0\u70ba\u9664\u4e86\u63e1\u6709\u5b57\u5143\uff0c\u6211\u5011\u66f4\u5e0c\u671b\u7684\u662f\u80fd\u8b80\u61c2\u9019\u90e8\u8457\u4f5c\u7684\u7d93\u5178\u4e4b\u8655\u3002\u7576\u7136\uff0c\u5176\u4ed6\u7269\u7a2e\u7684\u89e3\u5e8f\u5de5\u4f5c\u4e5f\u99ac\u4e0d\u505c\u8e44\u7684\u9032\u884c\uff0c\u5305\u62ec\u9175\u6bcd\u83cc\u3001\u7dda\u87f2\u3001\u679c\u8805\u3001\u5c0f\u9f20\u3001\u6c34\u7a3b\u4e43\u81f3\u9ed1\u7329\u7329\u7b49\u591a\u7a2e\u6a21\u5f0f\u751f\u7269\u4ea6\u9678\u7e8c\u5b8c\u6210\u3002<\/p>\n<p><span style=\"color: #008080;\"><strong>\u25cf\u4e0b\u4e00\u6b65\uff0c\u4eba\u5b9a\u52dd\u5929\uff1f<\/strong><\/span><\/p>\n<p>\u81ea1977\u5e74\u7b2c\u4e00\u7a2eDNA\u5b9a\u5e8f\u65b9\u6cd5\u51fa\u73fe\u81f3\u4eca\u5df240\u9918\u5e74\uff0c\u96a8\u5f8cPCR\u7684\u767c\u660e\u3001\u4eba\u985e\u57fa\u56e0\u5b9a\u5e8f\u5b8c\u6210\uff0c\u751a\u6642\u81f32015 \u5e74\uff0c\u7f8e\u570b\u516c\u53f8Veritas Genetics \u5ba3\u5e03\u80fd\u4ee5 1000 \u7f8e\u5143\u6210\u672c\u5b9a\u5e8f\u500b\u4eba\u57fa\u56e0\u7b49\u6b77\u7a0b\u4f86\u770b\uff0c\u57fa\u56e0\u5de5\u7a0b\u767c\u5c55\u901f\u5ea6\u5df2\u4e0d\u53ef\u540c\u65e5\u800c\u8a9e\uff0c\u50f9\u683c\u4e0a\u66f4\u662f\u65e5\u8da8\u4fbf\u5b9c<strong><sup>[2]<\/sup><\/strong>\u3002\u4f46\u4e0b\u4e00\u6b65\u5462\uff1f\u9762\u5c0d\u751f\u547d\u73fe\u8c61\u5982\u6b64\u8907\u96dc\uff0c\u7d55\u975e\u4e00\u5c0d\u4e00\u7c21\u55ae\u7dda\u6027\u7684\u4ea4\u4e92\u4f5c\u7528\u4e0b\uff0c\u4ee5\u57fa\u56e0\u70ba\u9996\u7684\u8ab2\u984c\uff0c\u986f\u7136\u4e26\u975e\u7d14\u7cb9\u5ba2\u89c0\u7684\u6280\u8853\u554f\u984c\u3002\u91cd\u8981\u7684\u662f\uff0c\u9019\u6a23\u7684\u6280\u8853\u8e8d\u9032\uff0c\u662f\u5426\u80fd\u70ba\u793e\u6703\u5e36\u4f86\u6b63\u5411\u5f71\u97ff\u548c\u6539\u8b8a\uff1f\u4e0d\u8ad6\u662f\u9762\u5c0d\u4eba\u53e3\u6210\u9577\u7684\u7ce7\u98df\u554f\u984c\u6216\u764c\u75c7\u7814\u7a76\u7b49\u96e3\u984c\uff0c\u76f8\u4fe1\u9019\u4e00\u672c\u672c\u5929\u66f8\u90fd\u9084\u6709\u4e00\u4e9b\u5c1a\u5f85\u4eba\u985e\u53c3\u900f\u7684\u5730\u65b9\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>---<\/p>\n<p>[\u8a3b1] Frederick Sanger\uff0c\u82f1\u570b\u751f\u7269\u5316\u5b78\u5bb6\uff0c\u662f\u7b2c\u4e00\u4f4d\u5169\u6b21\u5f97\u5230\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u5f97\u4e3b\u30021958\u5e74\u56e0\u78ba\u7acb\u80f0\u5cf6\u7d20\u7684\u5206\u5b50\u7d50\u69cb\uff0c\u7372\u9812\u7b2c\u4e00\u500b\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u3002<br \/>\n[\u8a3b2] <a href=\"https:\/\/www.oreilly.com\/ideas\/genomics-and-the-role-of-big-data-in-personalizing-the-healthcare-experience\">Genomics and the role of big data in personalizing the healthcare experience<\/a><\/p>\n<p>\u5ef6\u4f38\u66f8\u7c4d | \u300a\u57fa\u56e0\u7d44\uff1a\u4eba\u7a2e\u81ea\u50b323\u7ae0\u300b<br \/>\n\u5ef6\u4f38\u5716\u8868 | <a href=\"https:\/\/commons.wikimedia.org\/wiki\/File:Human_Genome_Project_Timeline_(26964377742).jpg\">Human Genome Project Timeline<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong>--\u672c\u6587\u6574\u7406\u81ea\uff1a107\/4\/14\u7531\u4e8e\u5b8f\u71e6\u8001\u5e2b\u5728\u81fa\u5927\u601d\u4eae\u9928\u570b\u969b\u6703\u8b70\u5ef3\u6240\u4e3b\u8b1b\u4e4b\u300c<a href=\"https:\/\/www.youtube.com\/watch?v=yVeluYjAGKA&amp;feature=youtu.be\" target=\"_blank\" rel=\"noopener noreferrer\">\u8b80\u4e00\u672c\u751f\u547d\u7684\u7121\u5b57\u5929\u66f8\uff1a\u89e3\u78bc\u57fa\u56e0\u9ad4DNA<\/a>\u300d\u6f14\u8b1b\u5167\u5bb9\u3002<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/line.me\/R\/ti\/p\/%40fcx6182w\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/scdn.line-apps.com\/n\/line_add_friends\/btn\/zh-Hant.png\" alt=\"\u52a0\u5165\u597d\u53cb\" width=\"212\" height=\"36\" border=\"0\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u25a0\u96fb\u8166\u4ee50\u82071\u7684\u6392\u5217\u65b9\u5f0f\u4f86\u5132\u5b58\u8cc7\u8a0a\uff0c\u751f\u7269\u5247\u662f\u7531\u6700\u57fa\u672c\u5b57\u5143A\u3001T\u3001C\u3001G\u56db\u7a2e\u9e7c\u57fa\u8a18\u9304\u4e14\u6c7a\u5b9a\u907a\u50b3\u7279\u5fb5\uff0c\u900f\u904e\u6392\u5217\u7d44\u5408\u7684\u8b8a\u5316\uff0c\u5f62\u6210\u5404\u5f0f\u6307\u4ee4\u9032\u800c\u5408\u6210\u86cb\u767d\u8cea\u8abf\u63a7\u5404\u7a2e\u7d30\u80de\u548c\u751f\u7269\u6a5f\u80fd\u3002\u800c\u9019\u6a23\u7684\u6a5f\u5236\u7a76\u7adf\u6709\u591a\u7cbe\u5bc6?\u6211\u5011\u53ef\u4ee5\u8a66\u8457\u9019\u9ebc\u60f3\uff1a\u5982\u679c\u5c07\u8eab\u4e0a46\u689d\u67d3\u8272\u9ad4\u7684DNA\u5f9e\u7d30\u80de\u4e2d\u62bd\u51fa\uff0c\u4e26\u5c07\u5b83\u5011\u6392\u5217\u8d77\u4f86\uff0c\u8a08\u7b97\u5f8c\u53ef\u90542-3\u516c\u5c3a\u9577\uff0c\u800c\u9019\u4e9b\u300c\u751f\u547d\u85cd\u5716\u300d\u7684\u9f90\u5927\u5bc6\u78bc\u8cc7\u8a0a\u5fc5\u9808\u7dca\u5bc6\u7e8f\u7e5e\u58d3\u7e2e\u81f3\u5728\u5e7e\u500b\u5fae\u7c73\u7684\u7d30\u80de\u6838\u5167\uff1b\u5982\u679c\u6211\u5011\u518d\u9032\u4e00\u6b65\u5c07\u5168\u8eab\u7d30\u80de\u7684DNA\u62c9\u76f4\u8a08\u7b97\uff0c\u90a3\u53ef\u662f\u76f8\u7576\u65bc\u5730\u7403\u5230\u592a\u967d\u4f86\u56de\u7d0470\u8d9f\u7684\u9577\u5ea6\u3002\u4f46\u5e7e\u767e\u842c\u5e74\u4f86\uff0c\u751f\u7269\u537b\u5f97\u4ee5\u900f\u904e\u9019\u4e9b\u9f90\u5927\u4e14\u7cbe\u78ba\u7684\u5bc6\u78bc\u7d00\u4e8b\uff0c\u5c07\u7279\u5fb5\u4ee3\u4ee3\u76f8\u50b3\uff0c\u4e26\u96a8\u8457\u6642\u9593\u6f14\u5316\uff0c\u9020\u5c31\u51fa\u5730\u7403\u751f\u7269\u5708\u7684\u591a\u8b8a\u6a23\u8c8c\u3002<\/p>\n","protected":false},"author":20,"featured_media":30969,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,3749],"tags":[3833,3834,3787,1809,3832],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2018\/05\/Flickr-@microphylum.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/30862"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=30862"}],"version-history":[{"count":7,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/30862\/revisions"}],"predecessor-version":[{"id":31994,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/30862\/revisions\/31994"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/30969"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=30862"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=30862"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=30862"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}