

{"id":27212,"date":"2017-05-04T00:12:22","date_gmt":"2017-05-03T16:12:22","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=27212"},"modified":"2018-07-29T09:21:17","modified_gmt":"2018-07-29T01:21:17","slug":"%e3%80%90%e5%a5%b3%e7%a7%91%e5%ad%b8%e5%ae%b6%e3%80%91%e9%96%8b%e5%95%9f%e6%a4%8d%e7%89%a9%e7%94%9f%e7%89%a9%e6%8a%80%e8%a1%93%e9%9d%a9%e5%91%bd%e7%9a%84%e8%be%b2%e6%a1%bf%e8%8f%8c%e5%a5%b3%e7%8e%8b","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=27212","title":{"rendered":"\u3010\u5973\u79d1\u5b78\u5bb6\u3011\u958b\u555f\u690d\u7269\u751f\u7269\u6280\u8853\u9769\u547d\u7684\u8fb2\u687f\u83cc\u5973\u738b\u2500\u2500\u746a\u9e97-\u6234\u723e\u2027\u5947\u723e\u9813"},"content":{"rendered":"<figure id=\"attachment_27213\" aria-describedby=\"caption-attachment-27213\" style=\"width: 450px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/www.youtube.com\/watch?v=qo8xvZuCEtg\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-27213\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2017\/02\/Mary-Dell-Chilton.jpg\" width=\"450\" height=\"312\" srcset=\"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2017\/02\/Mary-Dell-Chilton.jpg 980w, https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2017\/02\/Mary-Dell-Chilton-600x416.jpg 600w, https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2017\/02\/Mary-Dell-Chilton-800x555.jpg 800w\" sizes=\"(max-width: 450px) 100vw, 450px\" \/><\/a><figcaption id=\"caption-attachment-27213\" class=\"wp-caption-text\">\u00a9Syngenta<\/figcaption><\/figure>\n<p><strong>\u64b0\u6587\uff5c<\/strong>\u8449\u7da0\u8212<\/p>\n<p>\u746a\u9e97-\u6234\u723e\u2027\u5947\u723e\u9813(Mary-Dell Chilton)\u57281939\u5e74\u4e8c\u6708\u4e8c\u65e5\u751f\u65bc\u5370\u7b2c\u5b89\u7d0d\u5dde\u7684\u9996\u5e9c\u5370\u7b2c\u5b89\u7d0d\u6ce2\u5229\u65af\u3002\u9ad8\u4e2d\u6642\u4ee3\u7684\u5979\uff0c\u8ddf\u8a31\u591a\u7576\u6642\u7684\u5b78\u751f\u4e00\u6a23\uff0c\u8ff7\u4e0a\u4e86\u591c\u7a7a\u4e2d\u7480\u74a8\u7684\u661f\u661f\u3002\u5979\u52a0\u5165\u4e86\u829d\u52a0\u54e5\u963f\u5fb7\u52d2\u5929\u6587\u9928\uff08Adler Planetarium\uff09\u7684\u671b\u9060\u93e1\u88fd\u4f5c\u793e\u5718\uff0c\u5b78\u8457\u81ea\u88fd\u671b\u9060\u93e1\u3002\u56e0\u70ba\u5c0d\u81ea\u88fd\u671b\u9060\u93e1\u7684\u6548\u679c\u4e0d\u6eff\u610f\uff0c\u5979\u8a66\u8457\u9032\u884c\u6539\u826f\uff0c\u540c\u6642\u4e5f\u628a\u5979\u6539\u826f\u671b\u9060\u93e1\u7684\u60f3\u6cd5\u5beb\u6210\u8a08\u756b\u66f8\uff0c\u53c3\u52a01956\u5e74\u897f\u5c4b\u79d1\u5b78\u734e\uff08\u73fe\u6539\u540d\u70ba\u82f1\u7279\u723e\u79d1\u5b78\u734e\uff09\u7af6\u8cfd\u3002<\/p>\n<p>\u7af6\u8cfd\u96d6\u7136\u6c92\u6709\u5f97\u5230\u512a\u52dd<a href=\"#_ftn1\" name=\"_ftnref1\">[1]<\/a>\uff0c\u4f46\u4e5f\u9032\u5165\u4e86\u6700\u5f8c\u7684\u6c7a\u9078\u3002\u5728\u9078\u62d4\u7684\u904e\u7a0b\u4e2d\uff0c\u53c3\u8cfd\u8005\u8981\u5230\u73fe\u5834\u6f14\u793a\u4ed6\/\u5979\u7684\u60f3\u6cd5\uff0c\u9019\u500b\u7d93\u9a57\u8b93\u5979\u7b2c\u4e00\u6b21\u89ba\u5f97\u81ea\u5df1\u53ef\u80fd\u8207\u773e\u4e0d\u540c\uff1b\u65bc\u662f\u5230\u4e86\u4f0a\u5229\u8afe\u5927\u5b78\uff0c\u5979\u8feb\u4e0d\u53ca\u5f85\u5730\u60f3\u8981\u4e3b\u4fee\u5929\u6587\u5b78\u3002\u6c92\u60f3\u5230\uff0c\u56e0\u70ba\u8981\u4fee\u5929\u6587\u5b78\u7684\u5b78\u751f\u592a\u591a\uff0c\u5979\u88ab\u52f8\u9000\uff0c\u300c\u7b49\u5927\u4e8c\u518d\u4f86\u4fee\u300d<a href=\"#_ftn2\" name=\"_ftnref2\">[2]<\/a>\u3002<!--more--><\/p>\n<p>\u96d6\u8aaa\u7576\u6642\u5973\u6027\u8981\u8b80\u5927\u5b78\u5df2\u7d93\u4e0d\u662f\u554f\u984c\uff0c\u4f46\u5973\u751f\u9084\u662f\u5e38\u5e38\u6703\u88ab\u6392\u64e0\u3002\u88ab\u52f8\u9000\u7684\u5947\u723e\u9813\u6c7a\u5b9a\u4e0d\u518d\u7406\u6703\u5929\u6587\u5b78\u3001\u6539\u70ba\u4e3b\u4fee\u7269\u7406\uff0c\u4f46\u662f\u7269\u7406\u4e5f\u771f\u7684\u4e0d\u662f\u5979\u7684\u83dc\uff08\u5e38\u5e38\u5728\u8ab2\u5802\u4e0a\u7761\u8457\uff09\uff0c\u65bc\u662f\u6539\u70ba\u4e3b\u4fee\u5316\u5b78\u3002<\/p>\n<p>\u4f46\u4e0d\u8ad6\u662f\u5929\u6587\u3001\u7269\u7406\u3001\u9084\u662f\u5316\u5b78\uff0c\u90fd\u4e0d\u662f\u5979\u5fc3\u4e4b\u6240\u5411\u3002\u5230\u4e86\u7814\u7a76\u6240\uff0c\u5979\u624d\u627e\u5230\u4e86\u771f\u6b63\u8b93\u5979\u89ba\u5f97\u611f\u8208\u8da3\u7684\u5b78\u79d1\uff1a\u5206\u5b50\u751f\u7269\u5b78\u3002\u807d\u8457\u83ef\u751f\u3001\u514b\u91cc\u514b\u8207 DNA \u96d9\u80a1\u87ba\u65cb\u7684\u6545\u4e8b\u8b93\u5979\u8457\u8ff7\uff0c\u53ef\u662f\u7a76\u7adf\u5206\u5b50\u751f\u7269\u5b78\u80fd\u4f5c\u4ec0\u9ebc\u5462\uff1f<\/p>\n<p>\u6211\u5011\u73fe\u5728\u77e5\u9053\u5206\u5b50\u751f\u7269\u5b78\u6709\u975e\u5e38\u591a\u7684\u61c9\u7528\uff0c\u4f46\u57281960\u5e74\u4ee3\uff0c\u5927\u5bb6\u4e26\u4e0d\u6e05\u695a\u5206\u5b50\u751f\u7269\u5b78\u5230\u5e95\u80fd\u4f5c\u4ec0\u9ebc\uff1f\u65bc\u662f\uff0c\u5979\u6c7a\u5b9a\u5148\u5225\u6025\u8457\u627e\u5de5\u4f5c\u3002\u7562\u7adf\uff0c\u7576\u6642\u5728\u83ef\u76db\u9813\u5927\u5b78\u5b8c\u6210\u535a\u58eb\u5f8c\u7814\u7a76\u7684\u5979\uff0c\u5df2\u7d93\u7d50\u5a5a\u3001\u751f\u5b50\uff0c\u800c\u5979\u7684\u4e08\u592b\u662f\u5927\u5b78\u8001\u5e2b\uff0c\u85aa\u6c34\u990a\u5bb6\u6709\u9918\u3002\u65bc\u662f\uff0c\u5979\u5148\u5728\u83ef\u76db\u9813\u5927\u5b78\u7684\u5fae\u751f\u7269\u7cfb\u88e1\u9762\u517c\u5dee\uff0c\u6709\u7a7a\u6642\u4fbf\u5728\u4e08\u592b\u7684\u7814\u7a76\u5ba4\u88e1\u9762\u4f5c\u9ede\u5be6\u9a57\u3002<\/p>\n<p>\u5c31\u5728\u9019\u6642\u5019\uff0c\u4fee\u5979\u7684\u5fae\u751f\u7269\u5b78\u7684\u5b78\u751f\u62ff\u4e86\u4e00\u7bc7\u671f\u520a\u8ad6\u6587\u7d66\u5979\u770b\u3002\u8ad6\u6587\u4f86\u81ea\u65bc\u840a\u9813\u5927\u5b78\u7684\u4e00\u7bc7\u535a\u58eb\u8ad6\u6587\uff0c\u63d0\u5230\u690d\u7269\u88ab\u8fb2\u687f\u83cc\uff08<em>Agrobacterium tumefaciens<\/em>\uff09\u611f\u67d3\u5f8c\u6240\u7522\u751f\u7684\u51a0\u7624\uff08crown gall tumor\uff09\u7684DNA\u53ef\u4ee5\u8207\u8fb2\u687f\u83cc\u7684DNA\u96dc\u4ea4\uff0c\u56e0\u6b64\u4f5c\u8005\u8a8d\u70ba\u8fb2\u687f\u83cc\u5728\u611f\u67d3\u690d\u7269\u6642\uff0c\u6703\u5c07\u81ea\u5df1\u7684\u4e00\u6bb5\u57fa\u56e0\uff08DNA\uff09\u63d2\u5165\u690d\u7269\u4e2d\u3002<\/p>\n<p>\u7576\u6642\u5947\u723e\u9813\u7684\u53cd\u61c9\u662f\uff1a\u9019\u600e\u9ebc\u53ef\u80fd\uff01\u4f46\u662f\u904e\u5f8c\u518d\u60f3\u60f3\uff0c\u53c8\u89ba\u5f97\u883b\u6709\u610f\u601d\u7684\u3002\u65bc\u662f\u5979\u627e\u4e86\u5fae\u751f\u7269\u5b78\u5bb6\u5167\u65af\u7279\uff08Gene Nester\uff09\u8207\u690d\u7269\u75c5\u6bd2\u5b78\u5bb6\u9ad8\u767b\uff08Milt Gordon\uff09\u4e00\u8d77\u4f86\u7814\u7a76\u9019\u500b\u8ab2\u984c<a href=\"#_ftn3\" name=\"_ftnref3\">[3]<\/a>\u3002<\/p>\n<p>\u5be6\u9a57\u4e00\u958b\u59cb\u4e26\u4e0d\u6210\u529f\uff0c\u56e0\u70ba\u7576\u6642\u7684\u96dc\u4ea4\u6280\u8853\u9084\u975e\u5e38\u539f\u59cb\uff0c\u8981\u5728\u690d\u7269\u88e1\u9762\u5075\u6e2c\u5230\u4f4e\u65bc\u767e\u5206\u4e4b\u4e00\u4ee5\u4e0b\u7684 DNA \u5e8f\u5217\u5be6\u5728\u592a\u96e3\u4e86\uff08\u840a\u9813\u5927\u5b78\u7684\u7d50\u679c\u53ef\u80fd\u53ea\u662f\u96dc\u8a0a\uff09\u3002\u76f4\u5230\u5f8c\u4f86\uff0c\u8cd3\u5dde\u5927\u5b78\uff08University of Pennsylvania\uff09\u7684\u7814\u7a76\u5718\u968a\u767c\u73fe\uff0c\u7576\u4ed6\u5011\u628a\u8fb2\u687f\u83cc\u990a\u5728\u651d\u6c0f37\u5ea6\uff08\u8fb2\u687f\u83cc\u7684\u6700\u9069\u751f\u9577\u6eab\u5ea6\u662f\u651d\u6c0f28\u5ea6\uff09\u4e00\u6bb5\u6642\u9593\u4ee5\u5f8c\uff0c\u8fb2\u687f\u83cc\u4fbf\u6703\u5931\u53bb\u81f4\u7624\u6027\uff0c\u9019\u500b\u767c\u73fe\u610f\u5473\u8457\u8fb2\u687f\u83cc\u7684\u81f4\u7624\u56e0\u5b50\u4e26\u4e0d\u4f4d\u65bc\u5b83\u7684\u67d3\u8272\u9ad4\u4e0a\u3002<\/p>\n<p>\u7a76\u7adf\u651c\u5e36\u81f4\u7624\u56e0\u5b50\u7684\uff0c\u662f\u4e0d\u662f\u500b\u8cea\u9ad4\u5462\uff1f\u4e00\u958b\u59cb\u5206\u96e2\u8cea\u9ad4\u7684\u5617\u8a66\u4e26\u4e0d\u6210\u529f\uff0c\u76f4\u52301974\u5e74\u6bd4\u5229\u6642\u6839\u7279\u5927\u5b78\uff08Ghent University\uff09\u7684 Ivo Zaenen \u4ee5\u8517\u7cd6\u6fc3\u5ea6\u68af\u5ea6\u6210\u529f\u5206\u96e2\u51fa\u8fb2\u687f\u83cc\u7684\u8cea\u9ad4\uff08\u5f8c\u4f86\u9019\u500b\u8cea\u9ad4\u88ab\u7a31\u70baTi \uff08\u81f4\u7624\uff09\u8cea\u9ad4[Tumor-inducing (Ti) plasmid]\uff09\uff0c\u9019\u624d\u4e86\u89e3\u5230\u5176\u4ed6\u7814\u7a76\u5718\u968a\u5931\u6557\u7684\u539f\u56e0\uff1a\u8fb2\u687f\u83cc\u7684\u8cea\u9ad4\u592a\u5927\u4e86\uff01\u4e86\u89e3\u5230\u5982\u4f55\u5206\u96e2\u51fa\u8fb2\u687f\u83cc\u7684\u8cea\u9ad4\uff0c\u65bc\u662f\u5979\u5011\u8d95\u7dca\u5c07 Ti \u8cea\u9ad4\u5206\u96e2\u51fa\u4f86\uff0c\u91cd\u65b0\u88fd\u4f5c\u5206\u5b50\u6a19\u8a18\u3002\u7576\u6642\u70ba\u4e86\u8981\u6436\u5148\u767c\u8868\uff0c\u5947\u723e\u9813\u7684\u7814\u7a76\u5718\u968a\u711a\u818f\u7e7c\u6677\u5730\u5de5\u4f5c\u8457\uff0c\u4e0d\u7720\u4e0d\u4f11\u5730\u5de5\u4f5c\u4e86\u597d\u5e7e\u9031\uff0c\u624d\u7d42\u65bc\u5728\u690d\u7269\u7684\u51a0\u7624\u4e2d\u627e\u5230\u8fb2\u687f\u83cc\u7684 DNA<a href=\"#_ftn4\" name=\"_ftnref4\">[4]<\/a>\uff01<\/p>\n<p>\u8b49\u660e\u8fb2\u687f\u83cc\u6703\u628a\u81ea\u5df1\u7684 DNA \u63d2\u5165\u690d\u7269\uff0c\u63a5\u4e0b\u4f86\u7684\u5de5\u4f5c\u5c31\u662f\u8981\u770b\u770b\u5230\u5e95\u8f49\u9032\u53bb\u7684\u662f\u4ec0\u9ebc\u6a23\u7684 DNA\u3001\u4ee5\u53ca\u9019\u500b\u8cea\u9ad4\u4e0a\u7684\u54ea\u4e00\u6bb5\u5c0d\u8fb2\u687f\u83cc\u7684\u81f4\u7624\u6027\u6bd4\u8f03\u91cd\u8981\u3002\u5c07\u63d2\u5165\u690d\u7269\u7684\u7247\u6bb5\u9078\u6b96\u51fa\u4f86\u52a0\u4ee5\u5b9a\u5e8f\u3001\u4e26\u8207\u8fb2\u687f\u83cc\u7684Ti \u8cea\u9ad4\u5e8f\u5217\u6bd4\u5c0d\u5f8c\uff0c\u5f88\u5feb\u7684\u5c31\u767c\u73fe\u4e86\u8fb2\u687f\u83cc\u63d2\u5165\u690d\u7269\u7684 DNA \u7247\u6bb5\uff08\u88ab\u7a31\u70ba T-DNA\uff09\u662f\u4f4d\u65bc\u5de6\u908a\u754c\uff08LB\uff0cLeft Border\uff09\u8207\u53f3\u908a\u754c\uff08RB\uff0cRight Border\uff09\u4e4b\u9593\uff1b\u800c\u4ee5\u8f49\u4f4d\u56e0\u5b50\uff08transposon\uff09\u9032\u884c\u7a81\u8b8a\u7684\u7d50\u679c\u767c\u73fe\uff0c\u4e0d\u7ba1 T-DNA \u600e\u6a23\u5730\u88ab\u8f49\u4f4d\u56e0\u5b50\u7834\u58de\uff0c\u90fd\u4e0d\u5f71\u97ff\u5b83\u7684\u8f49\u5165\u3002\u9019\u500b\u7d50\u679c\u8b93\u5979\u5011\u610f\u8b58\u5230\uff1a\u6216\u8a31\u5979\u5011\u53ef\u4ee5\u7528\u4efb\u4f55\u57fa\u56e0\u4f86\u53d6\u4ee3 T-DNA\uff0c\u8fb2\u687f\u83cc\u90fd\u6703\u7167\u6a23\u628a\u5b83\u653e\u9032\u53bb\uff1f<\/p>\n<p>\u5947\u723e\u9813\u8207\u6839\u7279\u5927\u5b78\u7684\u7814\u7a76\u5718\u968a\u5f88\u5feb\u7684\u90fd\u8b49\u660e\u4e86\uff1a\u8fb2\u687f\u83cc\u7684\u78ba\u53ef\u4ee5\u628a\u4efb\u4f55\u4f4d\u65bcT-DNA\u5340\u57df\u7684DNA\u63d2\u5165\u690d\u7269\u3002\u9019\u6a23\u662f\u5426\u5c31\u4ee3\u8868\uff0c\u79d1\u5b78\u5bb6\u5011\u53ef\u4ee5\u8b93\u9019\u500b\u75c5\u539f\u83cc\u6416\u8eab\u4e00\u8b8a\uff0c\u6210\u70ba\u690d\u7269\u751f\u7269\u79d1\u6280\u7684\u8f09\u9ad4\u5462\uff1f<\/p>\n<p>\u7576\u7136\u662f\u53ef\u4ee5\u7684\uff01\u4e0d\u904e\uff0c\u9084\u6709\u6700\u5f8c\u4e00\u9053\u95dc\u5361\uff1a\u8fb2\u687f\u83cc\u7684\u8cea\u9ad4\u662f\u500b\u4e0a\u767e\u500b\u5343\u9e7c\u57fa\u5c0d\uff08kbps\uff0ckilo-base pairs\uff09\u7684\u9f90\u7136\u5927\u7269\uff0c\u9019\u9020\u6210\u64cd\u4f5c\u4e0a\u4e0d\u662f\u5f88\u65b9\u4fbf\u3002<\/p>\n<p>\u4f46\u662f\u5f88\u5feb\u5927\u5bb6\u5c31\u767c\u73fe\uff0cTi \u8cea\u9ad4\u53ef\u4ee5\u88ab\u5206\u6210\u5169\u500b\u3002\u5728\u4e4b\u524d\u4ee5\u8f49\u4f4d\u56e0\u5b50\u9032\u884c\u7a81\u8b8a\u6642\u767c\u73fe\u7684\u6bd2\u6027\u5340\uff08<em>vir <\/em>region\uff09\uff0c\u96d6\u7136\u662f\u8fb2\u687f\u83cc\u81f4\u7624\u6027\u5fc5\u5099\u7684\u57fa\u56e0\uff0c\u4f46\u5b83\u53ef\u4ee5\u88ab\u5206\u5230\u53e6\u4e00\u500b\u8cea\u9ad4\uff0c\u4e00\u9ede\u90fd\u4e0d\u5f71\u97ff T-DNA \u7684\u8f49\u5165\u3002\u9019\u9ebc\u4e00\u4f86\uff0c\u8cea\u9ad4\u4e00\u5206\u70ba\u4e8c\uff0c\u5269\u4e0b\u7684\u90e8\u5206\u5c0f\u4e86\u8a31\u591a\uff0c\u64cd\u4f5c\u4e0a\u5c31\u8b8a\u5f97\u66f4\u65b9\u4fbf\u4e86\uff01\u65bc\u662f\u5927\u5bb6\u719f\u6089\u7684\u300c\u4e8c\u5143\u8f09\u9ad4\u7cfb\u7d71\uff08binary vector\uff09\u300d\u5c31\u6b64\u8a95\u751f\u3002<\/p>\n<p>\u591a\u5e74\u5f8c\uff0c\u5947\u723e\u9813\u60f3\u5230\u9019\u6bb5\u6642\u5149\uff0c\u4ecd\u89ba\u5f97\u7576\u6642\u70ba\u4e86\u8981\u6436\u5148\u767c\u8868\uff0c\u6574\u500b\u7814\u7a76\u5718\u968a\u6295\u5165\u7684\u90a3\u7a2e\u72c0\u614b\uff0c\u8b93\u5979\u975e\u5e38\u61f7\u5ff5\u3002\u4f46\u7576\u6642\u7684\u767c\u73fe\uff0c\u5230\u6700\u5f8c\u5c0e\u81f4\u73fe\u5728\u7684\u57fa\u6539\u8ad6\u6230\uff0c\u5247\u662f\u5979\u6c92\u6709\u60f3\u5230\u7684\u3002\u57281983\u5e74\uff0c\u5979\u96e2\u958b\u4e86\u83ef\u76db\u9813\u5927\u5b78\u53bb\u5275\u8fa6\u81ea\u5df1\u7684\u690d\u7269\u751f\u7269\u79d1\u6280\u516c\u53f8\uff0c\u9019\u5bb6\u516c\u53f8\u5f8c\u4f86\u7d93\u904e\u4e00\u9023\u4e32\u7684\u8cfc\u4f75\u4e4b\u5f8c\uff0c\u6210\u70ba\u73fe\u5728\u7684\u5148\u6b63\u9054\uff08Syngenta\uff09\uff0c\u5168\u7403\u56db\u5927\u751f\u7269\u79d1\u6280\u516c\u53f8\u4e4b\u4e00\u3002<\/p>\n<p>\u8eab\u70ba\u5275\u8fa6\u4eba\u3001\u73fe\u5e7477\u6b72\u7684\u5947\u723e\u9813\u76ee\u524d\u4e0d\u50c5\u9084\u6c92\u6709\u9000\u4f11\uff0c\u5979\u70ba\u4e86\u8981\u57f9\u80b2\u5f8c\u9032\u7684\u79d1\u5b78\u4eba\u624d\uff0c\u6bcf\u5e74\u62db\u5f8525\u500b\u5b78\u751f\u5230\u5979\u5bb6\u4e2d\u4f5c\u5ba2\u3002\u9019\u4e9b\u5b78\u751f\u4e26\u4e0d\u53ea\u662f\u4f86\u73a9\uff0c\u4ed6\u5011\u4f4f\u5728\u5947\u723e\u9813\u5bb6\u88e1\u3001\u5230\u5947\u723e\u9813\u7684\u5be6\u9a57\u5ba4\u5de5\u4f5c\uff0c\u6642\u9593\u53ef\u4ee5\u5f9e\u4e09\u500b\u6708\u5230\u4e00\u5e74\u4e0d\u7b49\u3002\u5947\u723e\u9813\u8a8d\u70ba\uff0c\u9019\u6a23\u53ef\u4ee5\u8b93\u9019\u4e9b\u672a\u4f86\u7684\u79d1\u5b78\u5bb6\u66f4\u4e86\u89e3\uff0c\u7576\u4e00\u500b\u79d1\u5b78\u5bb6\u662f\u600e\u9ebc\u4e00\u56de\u4e8b\u3002\u56e0\u6b64\uff0c\u5979\u628a\u9019\u500b\u8a08\u756b\u7a31\u70ba\u300c\u5947\u723e\u9813\u5e0c\u723e\u9813\u8a08\u756b\u300d\uff08'The Chilton Hilton'\uff09<a href=\"#_ftn5\" name=\"_ftnref5\">[5]<\/a>\u3002<\/p>\n<p>\u5f9e1955\u5e74\u60f3\u8981\u88fd\u4f5c\u66f4\u597d\u7684\u5929\u6587\u671b\u9060\u93e1\u7684\u9ad8\u4e2d\u751f\u3001\u52301970\u5e74\u4ee3\u70ba\u4e86\u627e\u51fa\u8fb2\u687f\u83cc\u662f\u5426\u771f\u7684\u6703\u628a\u81ea\u5df1\u7684 DNA \u653e\u9032\u690d\u7269\u7684\u5b78\u8005\uff0c\u5947\u723e\u9813\u4e00\u76f4\u90fd\u662f\u8b93\u81ea\u5df1\u7684\u5fc3\u5e36\u9818\u8457\u81ea\u5df1\u5f80\u524d\u8d70\u3002\u8fd1\u5e74\u4f86\uff0c\u5979\u4e5f\u7372\u9812\u8a31\u591a\u734e\u9805\uff0c\u5176\u4e2d\u5305\u62ec2013\u4e16\u754c\u7ce7\u98df\u734e\uff0c\u4e26\u57282015\u5e74\u5217\u5165\u570b\u5bb6\u767c\u660e\u5bb6\u540d\u4eba\u5802\u3002\u96d6\u7136\u5c1a\u672a\u7372\u5f97\u8afe\u8c9d\u723e\u734e\uff0c\u4f46\u662f\u5947\u723e\u9813\u7684\u767c\u73fe\u5c0d\u5168\u4eba\u985e\u7684\u5f71\u97ff\u975e\u5e38\u91cd\u8981\u4e14\u6df1\u9060\u3002\u5c0d\u65bc\u8fd1\u5e74\u4f86\u57fa\u6539\u4f5c\u7269\u7684\u7a2e\u7a2e\u722d\u8b70\uff0c\u5979\u8a8d\u70ba\u8a31\u591a\u5176\u5be6\u662f\u91dd\u5c0d\u8457\u500b\u5225\u751f\u7269\u79d1\u6280\u516c\u53f8\u800c\u4f86\uff0c\u540c\u6642\u5979\u4e5f\u6307\u51fa\uff0c\u57fa\u6539\u751f\u7269\u6280\u8853\u7684\u6f5b\u529b\u5c1a\u672a\u88ab\u5b8c\u5168\u767c\u63ee\uff0c\u61c9\u8a72\u53ef\u4ee5\u6709\u6bd4\u6297\u75c5\u87f2\u5bb3\u3001\u6297\u6bba\u8349\u5291\u66f4\u597d\u7684\u61c9\u7528\uff0c\u4f86\u9020\u798f\u5168\u4eba\u985e\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"#_ftnref1\" name=\"_ftn1\">[1]<\/a> <a href=\"https:\/\/student.societyforscience.org\/science-talent-search-1956\" target=\"_blank\" rel=\"noopener noreferrer\">Science Talent Search 1956<\/a><\/p>\n<p><a href=\"#_ftnref2\" name=\"_ftn2\">[2]<\/a> Laura Vanderkam. 2008. \"<a href=\"https:\/\/www.scientificamerican.com\/article\/what-amuses-me-mary-dell-chilton\/\" target=\"_blank\" rel=\"noopener noreferrer\">What Amuses Me:\" Mary-Dell Chilton<\/a><\/p>\n<p>A 1956 Westinghouse finalist moves from optics and telescopes to revolutionizing agriculture. Scientific American.<\/p>\n<p><a href=\"#_ftnref3\" name=\"_ftn3\">[3]<\/a> Mary-Dell Chilton. Agrobacterium. A Memoir<\/p>\n<p>Plant Physiology. 2001. 125 (1): 9-14 (http:\/\/www.plantphysiol.org\/content\/125\/1\/9.full) doi: http:\/\/dx.doi.org\/10.1104\/pp.125.1.9<\/p>\n<p><a href=\"#_ftnref4\" name=\"_ftn4\">[4]<\/a><\/p>\n<p>Mary-Dell Chilton, Martin H. Drummond, Donald J. Merlo, Daniela Sciaky, Alice L. Montoya, Milton P. Gordon, Eugene W. Nester. 1977. Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell, Vol. 11, Issue 2, p263\u2013271. DOI: http:\/\/dx.doi.org\/10.1016\/0092-8674(77)90043-5<\/p>\n<p><a href=\"#_ftnref5\" name=\"_ftn5\">[5]<\/a> <a href=\"http:\/\/www.businessinsider.com\/mary-dell-chilton-on-feeding-the-world-and-gmo-controversy-2016-5\" target=\"_blank\" rel=\"noopener noreferrer\">The woman behind GMO technology on feeding the world and never-ending controversy.<\/a> Business Insider. Ariel Schwartz. May 5, 2016<\/p>\n<p><strong>--<\/strong><br \/>\n<span style=\"color: #333399;\"><strong>\u4f5c\u8005\uff1a\u8449\u7da0\u8212\u3000\u6148\u6fdf\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u52a9\u7406\u6559\u6388\uff0c\u79d1\u6559\u4e2d\u5fc3\u7279\u7d04\u5beb\u624b\uff0c\u5f9e\u4e8b\u79d1\u666e\u6587\u7ae0\u5beb\u4f5c\u3002<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/line.me\/R\/ti\/p\/%40fcx6182w\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/scdn.line-apps.com\/n\/line_add_friends\/btn\/zh-Hant.png\" alt=\"\u52a0\u5165\u597d\u53cb\" width=\"212\" height=\"36\" border=\"0\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u746a\u9e97-\u6234\u723e\u2027\u5947\u723e\u9813\u5f9e1955\u5e74\u60f3\u8981\u88fd\u4f5c\u66f4\u597d\u7684\u5929\u6587\u671b\u9060\u93e1\u7684\u9ad8\u4e2d\u751f\u3001\u52301970\u5e74\u4ee3\u70ba\u4e86\u627e\u51fa\u8fb2\u687f\u83cc\u662f\u5426\u771f\u7684\u6703\u628a\u81ea\u5df1\u7684 DNA \u653e\u9032\u690d\u7269\u7684\u5b78\u8005\uff0c\u5947\u723e\u9813\u4e00\u76f4\u90fd\u662f\u8b93\u81ea\u5df1\u7684\u5fc3\u5e36\u9818\u8457\u81ea\u5df1\u5f80\u524d\u8d70\u3002<br \/>\n\u96d6\u7136\u5c1a\u672a\u7372\u5f97\u8afe\u8c9d\u723e\u734e\uff0c\u4f46\u662f\u5947\u723e\u9813\u7684\u767c\u73fe\u5c0d\u5168\u4eba\u985e\u7684\u5f71\u97ff\u975e\u5e38\u91cd\u8981\u4e14\u6df1\u9060\u3002\u5c0d\u65bc\u8fd1\u5e74\u4f86\u57fa\u6539\u4f5c\u7269\u7684\u7a2e\u7a2e\u722d\u8b70\uff0c\u5979\u8a8d\u70ba\u8a31\u591a\u5176\u5be6\u662f\u91dd\u5c0d\u8457\u500b\u5225\u751f\u7269\u79d1\u6280\u516c\u53f8\u800c\u4f86\uff0c\u540c\u6642\u5979\u4e5f\u6307\u51fa\uff0c\u57fa\u6539\u751f\u7269\u6280\u8853\u7684\u6f5b\u529b\u5c1a\u672a\u88ab\u5b8c\u5168\u767c\u63ee\uff0c\u61c9\u8a72\u53ef\u4ee5\u6709\u6bd4\u6297\u75c5\u87f2\u5bb3\u3001\u6297\u6bba\u8349\u5291\u66f4\u597d\u7684\u61c9\u7528\uff0c\u4f86\u9020\u798f\u5168\u4eba\u985e\u3002<\/p>\n","protected":false},"author":20,"featured_media":27213,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,1042],"tags":[3228,230,3283,1606,3227],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2017\/02\/Mary-Dell-Chilton.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/27212"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27212"}],"version-history":[{"count":8,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/27212\/revisions"}],"predecessor-version":[{"id":31758,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/27212\/revisions\/31758"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/27213"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27212"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27212"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27212"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}