{"id":27189,"date":"2017-03-17T00:10:25","date_gmt":"2017-03-16T16:10:25","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=27189"},"modified":"2018-07-29T07:52:57","modified_gmt":"2018-07-28T23:52:57","slug":"crispr-%e5%9f%ba%e5%9b%a0%e7%b7%a8%e8%bc%af%e5%8b%95%e7%89%a9%e5%9c%92","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=27189","title":{"rendered":"CRISPR \u57fa\u56e0\u7de8\u8f2f\u52d5\u7269\u5712"},"content":{"rendered":"
\u5716\u7247\u4f86\u6e90:www.sciencemag.org<\/figcaption><\/figure>\n

\u64b0\u6587\uff5c<\/strong>\u99ac\u5343\u60e0<\/p>\n

CRISPR-Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u5c1a\u672a\u767c\u5c55\u4ee5\u524d\uff0c\u7814\u7a76\u4eba\u54e1\u50c5\u6539\u9020\u4e86\u5c11\u6578\u5e7e\u7a2e\u52d5\u7269\u7684\u57fa\u56e0\uff0c\u5176\u7a0b\u5e8f\u6548\u7387\u4f4e\u4e14\u9700\u8017\u8cbb\u8a31\u591a\u7684\u4eba\u529b\u53ca\u7d93\u8cbb\u3002\u5f97\u529b\u65bc\u76f8\u8f03\u800c\u8a00\u66f4\u52a0\u7cbe\u6e96\u7c21\u55ae\u7684CRISPR \u6280\u8853\uff0c\u7814\u7a76\u4eba\u54e1\u5f97\u4ee5\u6539\u9020\u66f4\u591a\u751f\u7269\u7269\u3b94\uff0c\u8a31\u591a\u4ee5\u5f80\u6c92\u8fa6\u6cd5\u505a\u7684\u8a08\u5283\uff0c\u90fd\u8b8a\u5f97\u5177\u6709\u53ef\u884c\u6027\u3002\u50c5\u50c5\u5728\u904e\u53bb\u5169\u5e74\u671f\u9593\uff0c\u79d1\u5b78\u5bb6\u5011\u5df2\u63d0\u51fa\u5728\u7334\u5b50\u3001\u9577\u6bdb\u8c61\u3001\u868a\u5b50\u4ee5\u53ca\u8a31\u591a\u7269\u7a2e\u9032\u884c\u57fa\u56e0\u6539\u9020\u7684\u5c55\u671b\uff0c\u61c9\u7528\u7684\u9818\u57df\u6a6b\u8de8\u8fb2\u696d\u3001\u88fd\u85e5\u4ee5\u53ca\u5fa9\u80b2\u7d76\u8de1\u7684\u7269\u7a2e\u3002\u7d93\u904e\u57fa\u56e0\u7de8\u8f2f\u6539\u9020\u51fa\u7684\u52d5\u7269\uff0c\u751a\u81f3\u6210\u70ba\u5e02\u9762\u4e0a\u8ca9\u552e\u7684\u5bf5\u7269\u3002\u7531\u65bc\u6b64\u6280\u8853\u76f8\u5c0d\u4f86\u8aaa\u5bb9\u6613\u9032\u884c\u800c\u4e14\u9020\u50f9\u4fbf\u5b9c\uff0c\u4e5f\u6709\u53ef\u80fd\u88ab\u4e0d\u6cd5\u4efd\u5b50\u5229\u7528\u4f86\u88fd\u9020\u5177\u7834\u58de\u6027\u7684\u751f\u7269\u6b66\u5668\uff0c\u800c\u5c07\u7528\u4f86\u7576\u4f5c\u98df\u54c1\u6216\u91ce\u653e\u81f3\u81ea\u7136\u754c\u7684\u7269\u7a2e\uff0c\u5fc5\u9808\u8003\u616e\u5176\u5b89\u5168\u6027\u548c\u5c0d\u751f\u614b\u7684\u5f71\u97ff\u3002\u5982\u4f55\u9069\u7576\u5730\u904b\u7528 CRISPR-Cas9 \u9019\u5f37\u5927\u7684\u5de5\u5177\uff0c\u4e26\u9032\u884c\u76f8\u7576\u7684\u7ba1\u63a7\u662f\u5f88\u91cd\u8981\u7684\uff0c\u6025\u9700\u7814\u7a76\u4eba\u54e1\u3001\u7ba1\u5236\u55ae\u4f4d\u4ee5\u53ca\u793e\u6703\u5927\u773e\u5171\u540c\u53c3\u8207\u8a0e\u8ad6\u3002<\/p>\n

\u25cf\u751f\u7269\u75be\u75c5\u7ba1\u63a7\u65b9\u9762\u7684\u61c9\u7528<\/strong><\/span><\/p>\n

\u5728\u8fb2\u6797\u696d\u4e0a\uff0c CRISPR-Cas9 \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u7684\u71b1\u9580\u61c9\u7528\u4e4b\u4e00\u5728\u65bc\u300c\u63d0\u5347\u6297\u75c5\u6027\u300d\u3002\u820a\u91d1\u5c71\u4e00\u751f\u6280\u4f01\u696d\u5bb6 Brian Gills \u7814\u7a76\u4e00\u7a2e\u6703\u6e05\u6f54\u8702\u7aa9\u7684\u871c\u8702\uff0c\u7260\u5011\u4e5f\u6703\u5c07\u53d7\u611f\u67d3\u751f\u75c5\u7684\u5e7c\u87f2\u642c\u96e2\uff0c\u5176\u65cf\u7fa4\u6bd4\u8f03\u4e0d\u6703\u53d7\u87ce\u3001\u9ef4\u83cc\u6216\u5176\u4ed6\u75c5\u539f\u9ad4\u5f71\u97ff\u3002Gills \u8a8d\u70ba\u5982\u679c\u53ef\u4ee5\u627e\u51fa\u6c7a\u5b9a\u6b64\u884c\u70ba\u7684\u57fa\u56e0\uff0c\u4ee5\u6b64\u70ba\u6a21\u677f\u53bb\u7de8\u8f2f\u5176\u4ed6\u7a2e\u985e\u871c\u8702\u7684\u57fa\u56e0\uff0c\u5c07\u53ef\u4f7f\u5176\u4ed6\u7a2e\u985e\u7684\u871c\u8702\u64c1\u6709\u66f4\u5065\u5eb7\u7684\u8702\u7aa9\u3002\u5f71\u97ff\u751f\u7269\u884c\u70ba\u7684\u56e0\u7d20\u901a\u5e38\u5f88\u8907\u96dc\uff0c\u76ee\u524d\u5c1a\u7121\u6cd5\u660e\u78ba\u5b9a\u51fa\u7279\u5b9a\u57fa\u56e0\u3002\u4e0d\u904e\u9019\u6a23\u7684\u8003\u616e\u4e26\u6ca1\u6709\u963b\u64cb\u79d1\u5b78\u5bb6\u5c07\u6b64\u6280\u8853\u61c9\u7528\u5728\u7ba1\u63a7\u751f\u7269\u75be\u75c5\u4e0a\u7684\u52aa\u529b\u3002\u53e6\u4e00\u7fa4\u79d1\u5b78\u5bb6\u8a66\u8457\u57fa\u56e0\u6539\u9020\u51fa\u80fd\u62b5\u6297\u75c5\u6bd2\u611f\u67d3\u7684\u8c6c\uff0c\u5c07\u8c6c\u7684\u514d\u75ab\u57fa\u56e0\u4fee\u6539\u5f97\u8f03\u63a5\u8fd1\u91ce\u8c6c\uff0c\u5e0c\u671b\u85c9\u6b64\u63d0\u9ad8\u8c6c\u7684\u514d\u75ab\u529b\u4f86\u62b5\u6297\u975e\u6d32\u8c6c\u761f\uff0c\u6216\u662f\u4fee\u6539\u8c6c\u7684\u7d30\u80de\u8868\u9762\u86cb\u767d\u8cea\uff0c\u4f7f\u611f\u67d3\u547c\u5438\u9053\u7684\u75c5\u6bd2\u7121\u6cd5\u9032\u5165\u8c6c\u9ad4\u5167\u3002\u53e6\u5916\u7de8\u8f2f\u725b\u7684\u57fa\u56e0\uff0c\u4f7f\u5176\u80fd\u62b5\u6297\u9020\u6210\u660f\u7761\u75c7\u72c0\u7684\u5bc4\u751f\u87f2\u3002\u9019\u4e9b\u7814\u7a76\u6210\u679c\u53ef\u4ee5\u964d\u4f4e\u8c6c\u548c\u725b\u611f\u67d3\u5f97\u75c5\u6a5f\u6703\uff0c\u6e1b\u5c11\u6bcf\u5e74\u755c\u7267\u696d\u7684\u640d\u5931\u3002<\/p>\n

\u653f\u5e9c\u55ae\u4f4d\u6b63\u5728\u8003\u616e\uff0c\u63a1\u7528\u539f\u5148\u7ba1\u5236\u57fa\u6539\u52d5\u7269\u7684\u6cd5\u898f\u4f86\u7ba1\u7406 CRISPR \u7de8\u8f2f\u52d5\u7269\u662f\u5426\u6070\u7576? \u56e0\u70ba CRISPR \u7de8\u8f2f\u52d5\u7269\u4e0d\u6703\u6709\u5176\u4ed6\u7269\u7a2e\u7684 DNA\uff0c\u6240\u4ee5\u4e26\u4e0d\u7b97\u662f\u57fa\u6539\u52d5\u7269\u3002<\/p>\n

\u25cf\u88fd\u85e5\u65b9\u9762\u7684\u61c9\u7528<\/strong><\/span><\/p>\n

\u5168\u4e16\u754c\u5927\u7d04\u6709\u767e\u5206\u4e4b\u4e8c\u7684\u5c0f\u5b69\u5c0d\u96de\u86cb\u904e\u654f\uff0c\u4ed6\u5011\u6c92\u6709\u8fa6\u6cd5\u65bd\u6253\u7531\u96de\u86cb\u88fd\u9020\u51fa\u4f86\u7684\u591a\u7a2e\u5e38\u898f\u75ab\u82d7\uff0c\u6fb3\u6d32\u5206\u5b50\u751f\u7269\u5b78\u5bb6 Timothy Doran \u7684\u5341\u4e00\u6b72\u5973\u5152\u5c31\u662f\u5176\u4e2d\u4e4b\u4e00\u3002\u4ed6\u76f8\u4fe1\u7528 CRISPR-Cas9 \u5f37\u5927\u7684\u57fa\u56e0\u7de8\u8f2f\u80fd\u529b\uff0c\u5c07\u53ef\u89e3\u6c7a\u9019\u500b\u554f\u984c\u3002<\/p>\n

\u5927\u90e8\u5206\u5c0d\u96de\u86cb\u904e\u654f\u7684\u4eba\u662f\u56e0\u70ba\u5c0d\u96de\u86cb\u86cb\u767d\u4e2d\u56db\u7a2e\u86cb\u767d\u8cea\u4e2d\u7684\u4e00\u7a2e\u904e\u654f\uff0cDoran \u7684\u69cb\u60f3\u662f\u5982\u679c\u80fd\u4ee5 CRISPR\u6280\u8853\u7cbe\u7d30\u7684\u8abf\u6574\u6539\u9020\u96de\u7684\u57fa\u56e0\u5e8f\u5217\uff0c\u4f7f\u6240\u88fd\u9020\u51fa\u7684\u86cb\u767d\u8cea\u4e0d\u6703\u555f\u52d5\u904e\u654f\u53cd\u61c9\uff0c\u4f46\u4ecd\u53ef\u7dad\u6301\u96de\u80da\u80ce\u7684\u767c\u80b2\uff0c\u4e4b\u5f8c\u53ef\u4ee5\u751f\u51fa\u4e0d\u6703\u5f15\u8d77\u904e\u654f\u53cd\u61c9\u7684\u96de\u86cb\u3002<\/p>\n

\u7531\u65bc\u9ce5\u985e\u7684\u53d7\u7cbe\u5375\u548c\u86cb\u9ec3\u7dca\u5bc6\u7d50\u5408\uff0c\u5982\u679c\u8981\u5728\u9ad4\u5916\u7de8\u8f2f\u57fa\u56e0\uff0c\u79fb\u51fa\u53d7\u7cbe\u5375\u7684\u904e\u7a0b\u6703\u7834\u58de\u96de\u80da\u80ce\u3002\u82e5\u662f\u8981\u5728\u6bcd\u96de\u9ad4\u5167\u9032\u884c\u57fa\u56e0\u7de8\u8f2f\uff0c\u53c8\u6709\u6280\u8853\u64cd\u4f5c\u4e0a\u7684\u56f0\u96e3\uff0c\u7b49\u5230\u86cb\u88ab\u751f\u51fa\u4f86\u5247\u5df2\u7d93\u932f\u904e\u4e86\u7de8\u8f2f\u57fa\u56e0\u7684\u6642\u6a5f\u3002\u65bc\u662f\u4ed6\u5011\u6539\u4ee5\u96de\u7684\u539f\u751f\u6b96\u7d30\u80de( primordial germ cells, PGCs)\u4f5c\u70ba\u57fa\u56e0\u7de8\u8f2f\u7684\u5c0d\u8c61\uff0c\u7de8\u8f2f\u5f8c\u518d\u653e\u56de\u767c\u80b2\u4e2d\u96de\u96bb\u7684\u8840\u6db2\u88cf\uff0c\u5f85\u5176\u6210\u719f\u5f8c\u6703\u6210\u70ba\u6709\u7279\u5b9a\u7de8\u8f2f\u57fa\u56e0\u7684\u7cbe\u5b50\u6216\u5375\u3002\u4ed6\u5011\u66f4\u8a08\u756b\u5c07 CRISPR-Cas9 \u6240\u9700\u5143\u4ef6\uff0c\u5148\u653e\u5230\u96de\u7684\u57fa\u56e0\u9ad4\u88cf\uff0c\u88fd\u9020\u51fa CRISPi \u96de\uff0c\u9019\u6a23\u53ef\u4ee5\u4f7f\u7de8\u8f2f\u7684\u7a0b\u5e8f\u66f4\u7c21\u5316\uff0c\u6709\u52a9\u65bc\u4ee5\u5bb6\u79bd\u5bb6\u755c\u4f86\u88fd\u9020\u85e5\u7269\u7684\u755c\u7522\u88fd\u85e5\u696d( farmaceuticals\u2019) \u767c\u5c55\u3002\u7ba1\u5236\u55ae\u4f4d\u8868\u793a\u9858\u610f\u8003\u616e\uff0c\u4f46\u662f\u9084\u9700\u8981\u76f8\u7576\u6642\u9593\u624d\u6709\u53ef\u80fd\u6838\u51c6\u4ee5\u57fa\u56e0\u7de8\u8f2f\u96de\u86cb\u4f86\u88fd\u85e5\u3002\u6b50\u76df\u65e9\u5728 2006\u5e74\u8a8d\u8b49\u4e86\u53ef\u4ee5\u751f\u7522\u6297\u51dd\u8840\u86cb\u767d\u8cea\u7f8a\u4e73\u7684\u57fa\u56e0\u6539\u9020\u5c71\u7f8a\uff0c\u4e4b\u5f8c 2009\u5e74\u7f8e\u570b\u98df\u54c1\u85e5\u7269\u7ba1\u7406\u5c40\u4e5f\u4e88\u4ee5\u8a8d\u8b49; \u800c\u5169\u500b\u55ae\u4f4d\u65bc 2015\u5e74\u4e5f\u90fd\u7d66\u4e88\u80fd\u88fd\u9020\u51fa\u81bd\u56fa\u9187\u85e5\u7269\u7684\u57fa\u56e0\u6539\u9020\u96de\u8a8d\u8b49\u3002<\/p>\n

\u25cf\u6d88\u9664\u52d5\u7269\u7d76\u7a2e\u554f\u984c<\/strong><\/span><\/p>\n

\u5927\u7d04\u56db\u5343\u5e74\u524d\u4eba\u6c23\u5019\u8b8a\u9077\u8207\u4eba\u985e\u6feb\u6355\u9020\u6210\u731b\u746a\u8c61\u6ec5\u7d55\u3002\u54c8\u4f5b\u91ab\u5b78\u9662 George Church \u8a66\u5716\u4f7f\u7528 CRISPR \u6280\u8853\u4f86\u5fa9\u539f\u7d55\u7a2e\u7684\u52d5\u7269\uff0c\u6b64\u8209\u5f15\u8d77\u773e\u4eba\u7684\u6ce8\u610f\u3002\u4ed6\u8a08\u756b\u5c07\u6fd2\u81e8\u7d76\u7a2e\u7684\u5370\u5ea6\u8c61\u900f\u904e\u57fa\u56e0\u6539\u9020\u65b9\u5f0f\u8b8a\u6210\u9577\u6bdb\u7684\u731b\u746a\u8c61\uff0c\u6216\u662f\u8b8a\u6210\u5177\u6709\u6297\u5bd2\u80fd\u529b\u7684\u5927\u8c61\uff0c\u7136\u5f8c\u5c07\u6539\u9020\u5f8c\u7684\u8c61\u653e\u990a\u65bc\u6709\u8db3\u5920\u6d3b\u52d5\u7a7a\u9593\u7684\u897f\u4f2f\u5229\u4e9e\u4fdd\u8b77\u5340\u30022015\u5e74\u829d\u52a0\u54e5\u5927\u5b78 Vincent Lynch \u7684\u7814\u7a76\u767c\u73fe\uff0c\u5c07\u7d30\u80de\u611f\u71b1\u53ca\u6bdb\u9aee\u751f\u9577\u7684\u57fa\u56e0\u52a0\u4ee5\u731b\u746a\u5316\u5f8c\uff0c\u6b64\u7d30\u80de\u53ef\u4ee5\u5728\u8f03\u4f4e\u6eab\u7684\u74b0\u5883\u4e0b\u751f\u9577\uff0c\u4e14\u5177\u6709\u6b64\u985e\u4f3c\u6539\u826f\u57fa\u56e0\u7684\u8001\u9f20\uff0c\u4e5f\u6bd4\u8f03\u559c\u6b61\u5f85\u5728\u63a7\u6eab\u57f9\u990a\u7bb1\u88cf\u8f03\u51b7\u7684\u5340\u57df\u4e2d\u3002\u800c\u54c8\u4f5b\u91ab\u5b78\u9662\u7684Church \u5247\u8868\u793a\u4ed6\u5df2\u7d93\u4fee\u6539\u4e86\u5341\u56db\u500b\u5927\u8c61\u80da\u80ce\u7684\u57fa\u56e0\uff0c\u7531\u65bc\u5c07\u57fa\u56e0\u7de8\u8f2f\u904e\u7684\u5927\u8c61\u80da\u80ce\u653e\u56de\u7015\u81e8\u7d55\u7a2e\u7684\u5370\u5ea6\u8c61\u5b50\u5bae\u88cf\u57f9\u80b2\uff0c\u88ab\u8996\u70ba\u662f\u9055\u53cd\u9053\u5fb7\u7684\u4e8b\uff0c\u6240\u4ee5\u4ed6\u5011\u6b63\u5728\u7814\u767c\u4eba\u9020\u7684\u5b50\u5bae\uff0c\u4f46\u662f\u5c1a\u672a\u6210\u529f\u3002\u6b64\u8209\u770b\u8d77\u4f86\u4f3c\u4e4e\u760b\u72c2\uff0c\u4f46\u662f\u5982\u679c\u7814\u7a76\u6210\u529f\uff0c\u5c07\u53ef\u63d0\u5347\u66f4\u591a\u52d5\u7269\u7684\u8010\u5bd2\u80fd\u529b\u3002<\/p>\n

\u52a0\u5dde\u5927\u5b78 Santa Cruz \u5206\u6821\u7684 Ben Novak \u5247\u4f01\u5716\u5fa9\u80b2\u5341\u4e5d\u4e16\u7d00\u56e0\u70ba\u4eba\u985e\u5927\u91cf\u7375\u6bba\u800c\u7d76\u7a2e\u7684\u5019\u9d3f\uff0c\u85c9\u7531\u6bd4\u8f03\u5716\u66f8\u9928\u6240\u4fdd\u5b58\u7684\u5207\u7247\u6a23\u672c\u548c\u73fe\u4ee3\u9d3f\u5b50\u7684 DNA\uff0c\u4ee5\u4fee\u6539\u539f\u751f\u6b96\u7d30\u80de\u65b9\u6cd5\uff0c\u4f86\u6539\u8b8a\u73fe\u4ee3\u9d3f\u5b50\u7684\u57fa\u56e0\uff0c\u4f7f\u7260\u5011\u66f4\u63a5\u8fd1\u5df2\u7d76\u7a2e\u7684\u5019\u9d3f\u3002\u5728\u6c92\u6709 CRISPR \u6280\u8853\u4e4b\u524d\uff0c\u4e00\u6b21\u4fee\u6539\u5e7e\u767e\u500b\u57fa\u56e0\u6839\u672c\u662f\u4e0d\u53ef\u80fd\u7684\u4e8b\uff0c\u6709\u4e86 CRISPR \u6280\u8853\uff0c\u4f7f\u6210\u529f\u7684\u6a5f\u6703\u5927\u589e\u3002<\/p>\n

\u25cf\u63a7\u5236\u651c\u5e36\u75c5\u539f\u7684\u8f09\u9ad4\u751f\u7269<\/span><\/strong><\/p>\n

\u6578\u5341\u5e74\u4f86\u7814\u7a76\u4eba\u54e1\u8a66\u5716\u4ee5\u4fee\u6539\u868a\u5b50\u57fa\u56e0\u7684\u65b9\u5f0f\u4f86\u9632\u6b62\u767b\u9769\u71b1\u548c\u8650\u75be\u7b49\u75be\u75c5\u7684\u6563\u64ad\uff0c CRISPR-Cas9 \u6280\u8853\u63d0\u4f9b\u4e86\u4e00\u500b\u65b0\u7684\u65b9\u5411\u3002<\/p>\n

\u52a0\u5dde\u5927\u5b78 Irvine \u5206\u6821\u7684\u5206\u5b50\u751f\u7269\u5b78\u5bb6 Anthony James \u57f9\u80b2\u4e86\u4e00\u7a2e\u5177\u6709\u57fa\u56e0\u9a45\u52d5\u7a0b\u5f0f(gene driver) \u7684\u868a\u5b50\uff0c\u9019\u7a2e\u5e36\u6709\u57fa\u56e0\u9a45\u52d5\u7a0b\u5f0f\u7684\u868a\u5b50\u53ef\u4ee5\u5c07\u6297\u7627\u75be\u57fa\u56e0\u50b3\u7d66\u7260\u7684\u5f8c\u4ee3\uff0c\u4e26\u78ba\u4fdd\u5e7e\u4e4e\u6240\u6709\u5b50\u4ee3\u90fd\u6703\u6709\u5169\u5957\u7de8\u8f2f\u904e\u7684\u57fa\u56e0\uff0c\u8b93\u6b64\u6297\u7627\u75be\u57fa\u56e0\u53ef\u4ee5\u5728\u65cf\u7fa4\u4e2d\u5feb\u901f\u50b3\u64ad\u30022015\u5e7410\u6708\u5e95\u53e6\u4e00\u7bc7\u7814\u7a76\u5831\u544a\u767c\u8868\u4e86\u4e00\u5957\u4e0d\u540c\u57fa\u56e0\u9a45\u52d5\u7a0b\u5f0f\uff0c\u7d93\u7531\u4fee\u6539\u57fa\u56e0\u4f7f\u5f97\u6bcd\u868a\u5b50\u7121\u6cd5\u751f\u80b2\uff0c\u4ee5\u6b64\u6d88\u6ec5\u868a\u5b50\u65cf\u7fa4\u3002\u9019\u5e7e\u5e74\u8332\u5361\u75c5\u6bd2\u7684\u7206\u767c\u4e8b\u4ef6\uff0c\u4f7f\u5f97\u5927\u5bb6\u5c0d\u6b64\u6280\u8853\u66f4\u6709\u8208\u8da3\uff0c\u76ee\u524d\u5df2\u6709\u5e7e\u500b\u5be6\u9a57\u5ba4\u8457\u624b\u7814\u767c\u76f8\u95dc\u57fa\u56e0\u9a45\u52d5\u7a0b\u5f0f\uff0c\u7528\u4ee5\u6d88\u6ec5\u8332\u5361\u75c5\u6bd2\u7684\u75c5\u5a92\u868a\u57c3\u53ca\u6591\u868a\u3002\u9664\u4e86\u57f9\u80b2\u50cf\u868a\u5b50\u9019\u985e\u6bd4\u8f03\u7c21\u55ae\u5bb9\u6613\u7814\u7a76\u7684\u651c\u5e36\u75c5\u539f\u7684\u8f09\u9ad4\u751f\u7269\u5916\uff0c\u79d1\u5b78\u5bb6\u4e5f\u6b63\u5728\u9032\u884c\u57f9\u80b2\u7121\u6cd5\u50b3\u64ad\u7d30\u83cc\u7684\u8731\u87f2\uff0c\u4ee5\u53ca\u7121\u6cd5\u50b3\u64ad\u5bc4\u751f\u87f2\u7684\u6c34\u87ba\u3002<\/p>\n

\u5728\u8655\u7406\u56b4\u91cd\u5a01\u8105\u4eba\u985e\u7684\u75be\u75c5\u554f\u984c\u6642\uff0c\u5c0d\u81ea\u7136\u754c\u7684\u5f71\u97ff\u4e5f\u662f\u6211\u5011\u5fc5\u9808\u8003\u616e\u7684\uff0c\u5f88\u591a\u79d1\u5b78\u5bb6\u958b\u59cb\u64d4\u6182\u91cb\u653e\u9019\u985e\u57fa\u56e0\u6539\u9020\u868a\u5b50\u5230\u81ea\u7136\u754c\u6709\u53ef\u80fd\u6703\u9020\u6210\u6211\u5011\u6c92\u6599\u5230\u6216\u672a\u77e5\u7684\u751f\u614b\u554f\u984c\u3002\u70ba\u6b64Church \u53ca\u4ed6\u7684\u540c\u4e8b\u958b\u767c\u4e86\u53e6\u4e00\u53cd\u57fa\u56e0\u9a45\u52d5\u7a0b\u5f0f\uff0c\u4e00\u5957\u53ef\u4ee5\u53cd\u5411\u6d88\u9664\u7de8\u8f2f\u7684\u65b9\u5f0f\u3002<\/p>\n

\u25cf\u589e\u52a0\u98df\u7269\u7522\u91cf<\/strong><\/span><\/p>\n

\u7f8e\u570b\u98df\u54c1\u85e5\u7269\u7ba1\u7406\u5c40\u7d93\u904e\u9577\u6642\u9593\u5be9\u6838\uff0c\u7d42\u65bc\u57282015\u5e74\u5341\u4e00\u6708\u6838\u51c6\u4e86\u7b2c\u4e00\u500b\u4f5c\u70ba\u4eba\u985e\u98df\u7269\u7684\u57fa\u56e0\u6539\u9020\u52d5\u7269\uff1a\u5feb\u901f\u751f\u9577\u7684\u9bad\u9b5a\uff08\u7531\u9ebb\u5dde AquaBounty \u516c\u53f8\u6240\u7814\u767c\u51fa\u4f86\uff09\u3002\u6709\u4e9b\u4eba\u64d4\u6182\u5982\u679c\u9019\u4e9b\u57fa\u6539\u9bad\u9b5a\u9003\u81f3\u91ce\u5916\uff0c\u5c07\u6703\u7834\u58de\u751f\u614b\u5e73\u8861\u3002\u9b5a\u985e\u79d1\u5b78\u5bb6 Rex Dunham \u91dd\u5c0d\u9019\u500b\u722d\u8b70\u505a\u51fa\u56de\u61c9\uff0c\u5982\u679c\u57fa\u6539\u9b5a\u53ef\u4ee5\u9054\u5230 100% \u7d76\u80b2\uff0c\u5373\u4f7f\u9003\u96e2\u4e5f\u6c92\u8fa6\u6cd5\u5728\u81ea\u7136\u754c\u7e41\u6b96\uff0c\u90a3\u9ebc\u5c0d\u751f\u614b\u5c31\u4e0d\u6703\u6709\u907a\u50b3\u4e0a\u7684\u5f71\u97ff\u3002\u4ee5\u7f8e\u570b\u6f01\u696d\u990a\u6b96\u6700\u591a\u7684\u9bf0\u9b5a\u4f5c\u70ba\u7814\u7a76\u5c0d\u8c61\uff0c\u4ed6\u4f7f\u7528 CRISPR \u6280\u8853\u6210\u529f\u5730\u6291\u5236\u4e86\u4e09\u500b\u751f\u6b96\u8cc0\u723e\u8499\u57fa\u56e0\uff0c\u5fc5\u9808\u7d93\u904e\u7279\u5b9a\u8cc0\u723e\u8499\u8655\u7406\u5f8c\uff0c\u624d\u53ef\u9032\u884c\u7e41\u884d\u3002\u540c\u6a23\u7684\u65b9\u6cd5\u53ef\u4ee5\u61c9\u7528\u5728\u4e0d\u540c\u7a2e\u7684\u9b5a\u985e\u3002<\/p>\n

CRISPR \u6280\u8853\u4e5f\u53ef\u4ee5\u6e1b\u5c11\u8fb2\u5834\u64b2\u6bba\u52d5\u7269\u7684\u6578\u76ee\u3002\u7531\u65bc\u6bcd\u725b\u7684\u7522\u8089\u91cf\u6bd4\u8f03\u4f4e\uff0c\u56e0\u6b64\u8fb2\u5834\u901a\u5e38\u5c07\u64b2\u6bba\u6bcd\u725b\u53ea\u7559\u4e0b\u516c\u725b\u3002\u52a0\u5dde\u5927\u5b78 Davis \u5206\u6821\u7684\u751f\u7269\u6280\u8853\u5bb6 Alison van Eenennaam \u5c07\u96c4\u6027 Y \u67d3\u8272\u9ad4\u4e0a\u63a7\u5236\u7537\u6027\u6027\u5fb5\u7684\u57fa\u56e0\u653e\u5230\u516c\u725b\u7cbe\u5b50\u7684 X \u67d3\u8272\u9ad4\u4e0a\uff0c\u5229\u7528\u6b64\u7279\u6b8a X \u67d3\u8272\u9ad4\u57f9\u80b2\u51fa\u6709\u516c\u725b\u7684\u7279\u5fb5\u7684\u6bcd\u725b\uff0c\u9019\u7a2e\u6bcd\u725b\u80fd\u9577\u51fa\u8f03\u591a\u808c\u8089\uff0c\u6e1b\u5c11\u88ab\u64b2\u6bba\u7684\u72c0\u6cc1\u3002<\/p>\n

\u5728\u96de\u86cb\u884c\u696d\u88e1\u7531\u65bc\u516c\u96de\u4e0d\u6703\u751f\u86cb\uff0c\u6240\u4ee5\u901a\u5e38\u525b\u5b75\u51fa\u4f86\u7684\u516c\u96de\u7576\u5929\u5c31\u6703\u88ab\u64b2\u6bba\uff0c\u6d1b\u6749\u78ef\u5dde\u7acb\u5927\u5b78 Tizard \u7684\u7814\u7a76\u5718\u968a\u5c07\u53ef\u4ee5\u8f49\u8b6f\u767c\u51fa\u7da0\u8272\u87a2\u5149\u7684\u86cb\u767d\u8cea\u57fa\u56e0\u526a\u63a5\u5230\u6027\u67d3\u8272\u9ad4\u4e0a\uff0c\u4f7f\u516c\u96de\u80da\u80ce\u5728\u7d2b\u5916\u7dda\u7167\u5c04\u4e0b\u6703\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff0c\u53ef\u4ee5\u5728\u5c0f\u96de\u5b75\u51fa\u524d\u5c07\u516c\u96de\u86cb\u6311\u51fa\uff0c\u8f49\u7528\u65bc\u75ab\u82d7\u7684\u751f\u7522\u3002<\/p>\n

\u5728\u904b\u9001\u725b\u96bb\u7684\u904e\u7a0b\u4e2d\uff0c\u5fc5\u9808\u5c07\u725b\u8d95\u5165\u8f03\u5c0f\u7a7a\u9593\uff0c\u5e38\u5e38\u6703\u9020\u6210\u725b\u96bb\u53d7\u50b7\uff0c\u5225\u662f\u9577\u89d2\u52d5\u7269\u66f4\u5bb9\u6613\u53d7\u50b7\u3002\u6240\u4ee5\u4e00\u822c\u990a\u725b\u7684\u4eba\u6703\u5728\u904b\u9001\u524d\u5c07\u725b\u89d2\u4ee5\u71d2\u3001\u5207\u5272\u6216\u5316\u5b78\u65b9\u6cd5\u53bb\u9664\uff0c\u6b64\u904e\u7a0b\u4e0d\u50c5\u52d5\u7269\u9700\u627f\u53d7\u75bc\u75db\uff0c\u5c0d\u57f7\u884c\u7684\u4eba\u4e5f\u5177\u5371\u96aa\u6027\u3002\u4f46\u662f\u82e5\u4ee5\u80b2\u7a2e\u65b9\u5f0f\u5c07\u512a\u8cea\u8089\u725b\u6216\u4e73\u725b\u548c\u5929\u751f\u7121\u89d2\u7684\u725b\u4ea4\u914d\uff0c\u7522\u751f\u7684\u8089\u725b\u5f8c\u4ee3\u7684\u8089\u54c1\u54c1\u8cea\u537b\u6703\u8b8a\u5dee\u3002\u5206\u5b50\u751f\u7269\u5b78\u5bb6 Scott Fahrenkrug \u540c\u6642\u4e5f\u662f Recombinetics in Saint Paul \u516c\u53f8\u7684\u5275\u8fa6\u4eba\uff0c\u5617\u8a66\u7528 CRIPR \u6280\u8853\uff0c\u5c07\u53bb\u6389\u725b\u89d2\u7684\u57fa\u56e0\u8207\u512a\u8cea\u8089\u725b\u57fa\u56e0\u526a\u63a5\u5728\u4e00\u8d77\uff0c\u8a72\u516c\u53f8\u76ee\u524d\u50c5\u57f9\u80b2\u4e86\u5169\u96bb\u7121\u89d2\u516c\u725b\u4e26\u98fc\u990a\u65bc\u52a0\u5dde\u5927\u5b78 Davis \u5206\u6821\uff0c\u672a\u4f86\u7b49\u4ed6\u5011\u6210\u9577\u5230\u53ef\u4ee5\u5b55\u80b2\u5b50\u4ee3\u5c31\u53ef\u77e5\u9053\u6210\u6548\u5982\u4f55\u3002\u61c9\u7528 CRISPR \u6280\u8853\u53ef\u4ee5\u4f7f\u755c\u7267\u696d\u66f4\u52a0\u7b26\u5408\u4eba\u9053\u7cbe\u795e\u3002<\/p>\n

\u25cf\u61c9\u7528\u65bc\u5bf5\u7269\u7684\u6539\u826f<\/span><\/strong><\/p>\n

2015\u5e74\u4e5d\u6708\uff0c\u57fa\u56e0\u516c\u53f8 BGI \u5728\u4e2d\u570b\u6df1\u5733\u7684\u7814\u8a0e\u6703\u4e0a\u767c\u8868\u4e86\u4ee4\u4eba\u9707\u9a5a\u3001\u9ad4\u91cd\u53ea\u670915\u516c\u65a4\u7684\u5fae\u578b\u8c6c\uff0c\u5927\u5c0f\u76f8\u7576\u65bc\u4e00\u689d\u6a19\u6e96\u7684\u81d8\u8178\u72d7\u3002BGI \u516c\u53f8\u539f\u672c\u662f\u8981\u57f9\u80b2\u6b64\u5fae\u578b\u8c6c\u4f5c\u70ba\u7814\u7a76\u7528\uff0c\u4f46\u662f\u5df2\u6c7a\u5b9a\u6539\u70ba\u4ee5\u7f8e\u91d1 1600 \u5143\u7684\u552e\u50f9\uff0c\u5c07\u5fae\u578b\u8c6c\u4f5c\u70ba\u65b0\u578b\u5bf5\u7269\u9032\u884c\u8ca9\u552e\uff0c\u4e26\u8a08\u5283\u5c07\u4f86\u80fd\u5920\u8b93\u8cb7\u5bb6\u6c7a\u5b9a\u5176\u6bdb\u76ae\u7684\u6a23\u5f0f\u3002\u9326\u9bc9\u80b2\u7a2e\u5728\u4e2d\u570b\u5df2\u6b77\u53f2\u4e45\u9060\uff0cBGI \u516c\u53f8\u7684\u8463\u4e8b Jian Wang \u6307\u51fa\u5373\u4f7f\u5091\u51fa\u7684\u80b2\u7a2e\u5c08\u5bb6\u901a\u5e38\u4e5f\u53ea\u80fd\u5728\u767e\u842c\u500b\u9b5a\u5375\u4e2d\uff0c\u57f9\u80b2\u51fa\u5c11\u6578\u5e7e\u689d\u51a0\u8ecd\u7b49\u7d1a\u7684\u9326\u9bc9\uff0c\u4f46 CRISPR \u6280\u8853\u53ef\u4ee5\u8b93\u4ed6\u5011\u6539\u826f\u9326\u9bc9\u7684\u5927\u5c0f\u3001\u984f\u8272\u548c\u82b1\u7d0b\uff0c\u66f4\u6e96\u78ba\u5730\u63a7\u5236\u9326\u9bc9\u7684\u82b1\u7d0b\uff0c\u540c\u6642\u4e5f\u57f9\u80b2\u4e0d\u9700\u8981\u5927\u578b\u9b5a\u6c60\u53ef\u4ee5\u9069\u61c9\u4e00\u822c\u5bb6\u5ead\u5f0f\u9b5a\u7f38\u7684\u9326\u9bc9\uff0c\u9810\u8a08\u5728 2017 \u6216 2018\u5e74\u5c31\u53ef\u4ee5\u4e0a\u5e02\uff0c\u540c\u6642\u4e5f\u6703\u6301\u7e8c\u958b\u767c\u4e0d\u540c\u7684\u5bf5\u7269\u9b5a\u3002<\/p>\n

\u6fb3\u6d32\u96ea\u68a8\u5927\u5b78\u7684\u907a\u50b3\u5b78\u5bb6 Claire Wade \u5247\u63d0\u51fa\u4ee5 CRISPR \u6280\u8853\u4f86\u6539\u826f\u72d7\uff0c\u5979\u7684\u7814\u7a76\u5718\u968a\u6bd4\u8f03\u4e0d\u540c\u54c1\u7a2e\u72d7\u7684\u57fa\u56e0\u5dee\u7570\uff0c\u5e0c\u671b\u53ef\u4ee5\u627e\u51fa\u5f71\u97ff\u72d7\u884c\u70ba\u548c\u7279\u5fb5\u7684\u57fa\u56e0\uff0c\u4f8b\u5982\u6c7a\u5b9a\u5176\u654f\u6377\u6027\u7684\u57fa\u56e0\u3002\u97d3\u570b\u9996\u723e\u7684Sooam \u751f\u6280\u516c\u53f8\u4ee5\u8981\u50f9\u7f8e\u91d1\u5341\u842c\u5143\u514b\u9686\u5df2\u6b7b\u5bf5\u7269\u805e\u540d\uff0c\u8fd1\u4f86\u4ed6\u5011\u958b\u59cb\u5c0d CRISP \u6280\u8853\u6709\u8208\u8da3\uff0c\u8a08\u5283\u4ee5\u6b64\u6280\u8853\u4f86\u6539\u5584\u5c0e\u76f2\u72d7\u548c\u7267\u72ac\u7b49\u529f\u80fd\u72ac\u7684\u5de5\u4f5c\u80fd\u529b\u3002<\/p>\n

\u54c8\u4f5b\u5927\u5b78\u7684\u751f\u7269\u502b\u7406\u5bb6\u7684 Jeantine Lunshof \u6307\u51fa\uff0c\u70ba\u4e86\u6eff\u8db3\u4eba\u5011\u617e\u671b\u800c\u53bb\u6539\u9020\u52d5\u7269\u5916\u8c8c\u53ef\u80fd\u6703\u50b7\u5bb3\u5230\u52d5\u7269\u3002\u4f46\u5979\u4e5f\u627f\u8a8d\uff0c\u5176\u5be6 CRISPR \u4f5c\u6cd5\u548c\u5e7e\u4e16\u7d00\u4ee5\u4f86\u4eba\u985e\u7528\u4f86\u5f37\u5316\u755c\u990a\u52d5\u7269\u548c\u5bf5\u7269\u7684\u67d0\u4e9b\u7279\u5b9a\u7279\u8cea\u7684\u8fd1\u89aa\u4ea4\u914d\u65b9\u6cd5\uff0c\u5c0d\u52d5\u7269\u800c\u8a00\u672c\u8cea\u4e0a\u4e26\u6c92\u4ec0\u9ebc\u4e0d\u540c\u3002\u800c CRISPR \u6280\u8853\u751a\u81f3\u53ef\u4ee5\u66f4\u9032\u4e00\u6b65\u89e3\u6c7a\u4e00\u4e9b\u554f\u984c\uff0c\u4f8b\u5982\u6539\u5584\u6578\u5341\u5e74\u4f86\u56e0\u8fd1\u89aa\u4ea4\u914d\u6cd5\u6240\u9020\u6210\u7684\u72d7\u7684\u81c0\u90e8\u554f\u984c\u3002<\/p>\n

\u25cf\u52d5\u7269\u5be6\u9a57\u6a21\u5f0f\u7684\u5efa\u7acb<\/span><\/strong><\/p>\n

\u9577\u4e45\u4ee5\u4f86\u7528\u4ee5\u7814\u7a76\u4eba\u985e\u611f\u5192\u75c5\u6bd2\u7684\u5be6\u9a57\u52d5\u7269\u662f\u8c82\uff0c\u56e0\u70ba\u611f\u5192\u75c5\u6bd2\u6703\u5728\u8c82\u7684\u547c\u5438\u9053\u8907\u88fd\uff0c\u8c82\u6709\u6642\u4e5f\u6703\u6709\u6253\u5674\u568f\u7684\u75c7\u72c0\uff0c\u53ef\u7528\u4f86\u7814\u7a76\u75c5\u6bd2\u50b3\u64ad\u3002\u5317\u4eac\u4e2d\u570b\u79d1\u5b78\u7814\u7a76\u9662 Xiaoqun Wang \u5229\u7528 CRISPR \u6280\u8853\u7de8\u8f2f\u53c3\u8207\u8c82\u5927\u8166\u767c\u80b2\u7684\u57fa\u56e0\u7fa4\uff0c\u4ee5\u6b64\u4f86\u7814\u7a76\u4e26\u6539\u8b8a\u52d5\u7269\u5c0d\u611f\u5192\u75c5\u6bd2\u7684\u611f\u53d7\u6027\uff0c\u540c\u6642\u4ed6\u5011\u4e5f\u5c07\u6b64\u52d5\u7269\u5be6\u9a57\u6a21\u5f0f\u63d0\u4f9b\u7d66\u5176\u4ed6\u7814\u7a76\u50b3\u67d3\u75be\u75c5\u7684\u7814\u7a76\u4eba\u54e1\u3002<\/p>\n

\u884c\u70ba\u5b78\u7814\u7a76\u4eba\u54e1\u5247\u662f\u5c0d\u548c\u4eba\u985e\u6bd4\u8f03\u63a5\u8fd1\u7684\u7d68\u7334\u548c\u7334\u5b50\u57fa\u56e0\u7de8\u8f2f\u52d5\u7269\u611f\u8208\u8da3\uff0c\u7279\u5225\u662f\u4e2d\u570b\u548c\u65e5\u672c\u30022016\u5e74\u4e00\u6708\u5728\u4e0a\u6d77\u7684\u4e2d\u570b\u570b\u5bb6\u79d1\u5b78\u9662\u795e\u7d93\u79d1\u5b78\u5bb6 \u4ec7\u5b50\u9f99\u7684\u7814\u7a76\u5718\u968a\u767c\u8868\u4ee5 CRISPR \u6280\u8853\u5f15\u9032\u7a81\u8b8a\uff08\u8a3b\uff09\u5230\u5f4c\u7334\u7684 MECP2 \u57fa\u56e0\uff0c\u6b64\u57fa\u56e0\u548c\u795e\u7d93\u767c\u5c55\u969c\u7919 Rett \u75c7\u72c0\u76f8\u95dc\uff0c\u7d93\u904e\u57fa\u56e0\u7de8\u8f2f\u7684\u5f4c\u7334\u8868\u73fe\u51fa\u6cdb\u81ea\u9589\u75c7\u969c\u7919\uff0c\u5305\u62ec\u91cd\u8907\u7684\u884c\u70ba\u548c\u907f\u514d\u793e\u4ea4\u63a5\u89f8\u3002\u4f46\u662f\u907a\u50b3\u5b78\u5bb6 Anthony Chan \u544a\u8aa1\u7814\u7a76\u4eba\u54e1\u5011\uff0c\u5728\u5efa\u7acb\u52d5\u7269\u5be6\u9a57\u6a21\u5f0f\u6642\u61c9\u8a72\u8981\u8b39\u614e\u8003\u616e\u9053\u5fb7\u554f\u984c\uff0c\u4f8b\u5982\u9078\u7528\u8001\u9f20\u9032\u884c\u5be6\u9a57\u5373\u53ef\uff0c\u4e26\u975e\u6240\u6709\u75be\u75c5\u90fd\u9700\u8981\u4f7f\u7528\u9748\u9577\u985e\u52d5\u7269\u4f86\u9032\u884c\u52d5\u7269\u5be6\u9a57\u3002<\/p>\n

\u57fa\u790e\u795e\u7d93\u79d1\u5b78\u4e5f\u53ef\u4ee5\u5f9e\u65b0\u7684\u52d5\u7269\u5be6\u9a57\u6a21\u5f0f\u5f97\u5230\u52a9\u76ca\uff0c\u9ebb\u7701\u7406\u5de5\u5b78\u9662\u7684\u795e\u7d93\u751f\u7269\u5b78\u5bb6 Ed Biden \u57f9\u80b2\u4e00\u6279\u5168\u4e16\u754c\u6700\u5c0f\u7684\u54fa\u4e73\u985e\u52d5\u7269\u6a39\u9f29\uff0c\u6a39\u9f29\u7684\u8166\u5c0f\u5230\u53ef\u4ee5\u5728\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\uff0c\u7d93\u57fa\u56e0\u7de8\u8f2f\u7684\u8166\u90e8\uff0c\u5176\u795e\u7d93\u5143\u5728\u767c\u51fa\u8a0a\u865f\u6642\u6703\u51fa\u73fe\u9583\u5149\uff0c\u53ef\u8b93\u7814\u7a76\u4eba\u54e1\u7814\u7a76\u52d5\u7269\u7684\u8166\u90e8\u7684\u5373\u6642\u8b8a\u5316\u3002<\/p>\n

CRISPR \u52d5\u7269\u5712\u5feb\u901f\u64f4\u5f35\uff0c\u672a\u4f86\u8a72\u5f80\u54ea\u500b\u65b9\u5411\u7e7c\u7e8c\u524d\u9032\uff1f\u6b64\u9818\u57df\u52e2\u5fc5\u9762\u5c0d\u524d\u4e00\u4ee3\u57fa\u56e0\u6539\u9020\u690d\u7269\u548c\u52d5\u7269\u6240\u5f15\u767c\u5927\u773e\u53cd\u5f48\u7684\u56f0\u64fe\uff0c\u70ba\u4e86\u907f\u514d\u722d\u8b70\u518d\u5ea6\u64f4\u5927\uff0c\u79d1\u5b78\u5bb6\u5011\u6709\u8cac\u4efb\u4e5f\u5fc5\u9808\u548c\u5927\u773e\u8aac\u660e CRISPR \u57fa\u56e0\u7de8\u8f2f\u6280\u8853\u5728\u751f\u7269\u61c9\u7528\u7684\u512a\u9ede\u751a\u81f3\u6f5b\u5728\u5371\u6a5f\uff0c\u6bcf\u500b\u4eba\u90fd\u61c9\u8a72\u8b39\u614e\u601d\u8003\u4ec0\u9ebc\u662f\u6211\u5011\u80fd\u638c\u63a7\u5229\u7528\uff0c\u4ec0\u9ebc\u662f\u6211\u5011\u80fd\u53bb\u505a\u4e5f\u80fd\u64c1\u6709\u7684\u3002<\/p>\n

 <\/p>\n

\u8a3b\uff1a\u7a81\u8b8a\u662f\u5c07 MECP2\u7684 DNA \u5e8f\u5217\u6539\u6389\uff0c\u4f7f\u5176\u6240\u505a\u51fa\u7684\u86cb\u767d\u5931\u53bb\u539f\u529f\u80fd<\/p>\n

 <\/p>\n

\u53c3\u8003\u6587\u737b:<\/strong><\/p>\n

1.Reardon, S. Welcome to the CRISPR zoo. Nature 531, 160-163, doi:10.1038\/531160a (2016).
\n2.Dhanapala, P., Doran, T., Tang, M. L. & Suphioglu, C. Production and immunological analysis of IgE reactive recombinant egg white allergens expressed in Escherichia coli. Molecular immunology 65, 104-112, doi:10.1016\/j.molimm.2015.01.006 (2015).
\n3.Lynch, V. J. et al. Elephantid Genomes Reveal the Molecular Bases of Woolly Mammoth Adaptations to the Arctic. Cell reports 12, 217-228, doi:10.1016\/j.celrep.2015.06.027 (2015).
\n4.Gantz, V. M. et al. Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi. Proceedings of the National Academy of Sciences of the United States of America 112, E6736-6743, doi:10.1073\/pnas.1521077112 (2015).
\n5.Tan, W. et al. Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proceedings of the National Academy of Sciences of the United States of America 110, 16526-16531, doi:10.1073\/pnas.1310478110 (2013).
\n6.Arnott, E. R. et al. Strong selection for behavioural resilience in Australian stock working dogs identified by selective sweep analysis. Canine genetics and epidemiology 2, 6, doi:10.1186\/s40575-015-0017-6 (2015).
\n7.Liu, Z. et al. Autism-like behaviours and germline transmission in transgenic monkeys overexpressing MeCP2. Nature 530, 98-102, doi:10.1038\/nature16533 (2016).<\/p>\n

 <\/p>\n

\"\u52a0\u5165\u597d\u53cb\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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