{"id":27124,"date":"2017-03-10T00:14:42","date_gmt":"2017-03-09T16:14:42","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=27124"},"modified":"2018-07-29T07:55:32","modified_gmt":"2018-07-28T23:55:32","slug":"%e5%93%aa%e5%80%8b%e7%96%be%e7%97%85%e6%98%afcrispr%e6%ac%bd%e9%bb%9e%e7%9a%84%e5%b9%b8%e9%81%8b%e5%85%92%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=27124","title":{"rendered":"\u54ea\u500b\u75be\u75c5\u662fCRISPR\u6b3d\u9ede\u7684\u5e78\u904b\u5152\uff1f"},"content":{"rendered":"

\u25a0 CRISPR \u6240\u5e36\u4f86\u7684\u57fa\u56e0\u6cbb\u7642\u9858\u666f\u5982\u6b64\u8b93\u4eba\u671f\u5f85\uff0c\u54ea\u4e9b\u75be\u75c5\u6703\u662f\u8b93\u79d1\u5b78\u5bb6\u8e8d\u8e8d\u6b32\u8a66\u7684\u5e78\u904b\u5152\uff0c\u6210\u70ba\u9019\u5283\u6642\u4ee3\u91ab\u7642\u65b0\u7b56\u7565\u7684\u6572\u9580\u78da\u5462\uff1f<\/strong><\/span><\/p>\n

\"\u00a9
\u00a9 www.missopen.com\/<\/figcaption><\/figure>\n

\u64b0\u6587\uff5c<\/strong>\u99f1\u5b9b\u7433<\/p>\n

\u7d30\u80de\u6838\u5167\u5e36\u6709\u7684\u57fa\u56e0\u9ad4\u8a0a\u606f\u50cf\u662f\u5df2\u7d93\u5beb\u597d\u7684\u5287\u672c\uff0c\u5de6\u53f3\u8457\u7d30\u80de\u7684\u8868\u73fe\u578b\u614b\u8207\u529f\u80fd\u3002\u96d6\u7136\uff0c\u6211\u5011\u7121\u6cd5\u5c0d\u9019\u5957\u5287\u672c\u88e1\u7684\u6240\u6709\u7d30\u7bc0\u90fd\u77e5\u4e4b\u751a\u8a73\uff0c\u4f46\u5c0d\u5287\u672c\u88e1\u7684\u67d0\u4e9b\u7ae0\u7bc0\u6bb5\u843d\uff0c\u5012\u662f\u5c07\u5e95\u7d30\u6478\u5f97\u6975\u5176\u900f\u5fb9\u3002\u76ee\u524d\u76f8\u95dc\u79d1\u5b78\u7814\u7a76\uff0c\u4e0d\u4f46\u77e5\u9053\u54ea\u4e00\u6bb5\u57fa\u56e0\u5e8f\u5217\u51fa\u4e86\u932f\uff0c\u6703\u5c0e\u81f4\u86cb\u767d\u8cea\u529f\u80fd\u4e0d\u5168\u6216\u8abf\u63a7\u5931\u5e38\uff1b\u9084\u77e5\u9053\u8a72\u5982\u4f55\u7d44\u5408 A\u3001T\u3001C\u3001G \u9e7c\u57fa\u5c0d\uff0c\u597d\u8b93\u8868\u73fe\u51fa\u4f86\u7684\u86cb\u767d\u8cea\u80fd\u57f7\u884c\u7279\u5b9a\u529f\u80fd\u3002\u9ed8\u9ed8\u5730\uff0c\u79d1\u5b78\u5bb6\u4f3c\u4e4e\u5b78\u6703\u4e86\u53e6\u4e00\u7a2e\u8a9e\u8a00\uff0c\u5b78\u6703\u6e9d\u901a\u8457\u57fa\u56e0\u5e8f\u5217\uff0c\u4f86\u89e3\u78bc\u86cb\u767d\u8cea\u8868\u73fe\u3001\u9084\u6709\u7d30\u80de\u529f\u80fd\u8207\u578b\u614b\u3002<\/p>\n

\u5982\u679c\u80fd\u5920\u76f4\u63a5\u5728\u7d30\u80de\u5167\u57fa\u56e0\u9ad4\u4e0a\u300c\u7de8\u8f2f\u300d\u57fa\u56e0\u5e8f\u5217\uff0c\u4e0d\u8ad6\u662f\u5c07\u7a81\u8b8a\u800c\u5c0e\u81f4\u7684\u932f\u8aa4\u57fa\u56e0\u8a0a\u606f\u52a0\u4ee5\u66f4\u6b63\uff0c\u6216\u662f\u589e\u6dfb\u6709\u7528\u7684\u8a0a\u606f\uff0c\u8b93\u7d30\u80de\u80fd\u5920\u626d\u8f49\u75c5\u7406\u4e0a\u7684\u52a3\u52e2\uff0c\u90fd\u662f\u300c\u57fa\u56e0\u6cbb\u7642\u300d\u7684\u65b0\u5929\u5802\u6a02\u5712\u3002\u4f46\u7e31\u7136\u79d1\u5b78\u5bb6\u5df2\u7d93\u77e5\u9053\u5287\u672c\u8a72\u5982\u4f55\u6539\u5beb\uff0c\u537b\u4e00\u76f4\u82e6\u65bc\u5de7\u5a66\u96e3\u70ba\u7121\u7c73\u4e4b\u708a\u3002\u8981\u76f4\u63a5\u7de8\u8f2f\u7d30\u80de\u5167\u7684\u57fa\u56e0\u8a0a\u606f\uff0c\u662f\u96e3\u5982\u767b\u5929\u7684\u6311\u6230\u3002<\/p>\n

\u4f46\u8fd1\u5e7e\u5e74\u9010\u6f38\u6210\u719f\u7684 CRISPR \u65b0\u6280\u8853\uff0c\u5012\u662f\u50cf\u7d42\u65bc\u7761\u9192\u7684\u795e\u71c8\u5167\u7cbe\u9748\uff0c\u4f3c\u4e4e\u5df2\u7d93\u96a8\u6642\u6e96\u5099\u597d\u5439\u97ff\u300c\u57fa\u56e0\u6cbb\u7642\u300d\u65b0\u7d00\u5143\u4f86\u81e8\u7684\u865f\u89d2\u3002\u5728\u85e5\u5ee0\u3001\u751f\u6280\u516c\u53f8\u8207\u5b78\u8853\u754c\u90fd\u8e8d\u8e8d\u6b32\u8a66\u7684\u6b64\u523b\uff0c\u662f\u54ea\u4e9b\u75be\u75c5\u88ab CRISPR \u9019\u96bb\u795e\u71c8\u7cbe\u9748\u6070\u6070\u597d\u9078\u4e2d\uff0c\u64d4\u7db1\u5283\u6642\u4ee3\u91ab\u7642\u7684\u9996\u6279\u8a66\u91d1\u77f3\u5462\uff1f<\/p>\n

\u5176\u4e00\uff0c\u5c31\u662f\u591a\u5e74\u4f86\u76e4\u8e1e\u5341\u5927\u6b7b\u56e0\u4e4b\u9996\uff0c\u7576\u4ec1\u4e0d\u8b93\u7a69\u5750\u7b2c\u4e00\u540d\u7684\u7684\u60e1\u6027\u816b\u7624\u3002<\/p>\n

2016\u5e74\u5341\u6708\u5e95\uff0c\u4e2d\u570b\u5c31\u5df2\u4e00\u99ac\u7576\u5148\u5730\u5c07 CRISPR \u7528\u65bc\u81e8\u5e8a\u6cbb\u7642\u7684\u53ef\u80fd\u6027\uff0c\u7531\u79d1\u5b78\u5bb6\u7684\u75f4\u5922\u5e36\u5230\u73fe\u5be6\u4e2d\u3002\u4e2d\u570b\u6210\u90fd\u56db\u5ddd\u5927\u5b78\u7684\u76e7\u923e\u535a\u58eb\uff0c\u5728\u83ef\u897f\u91ab\u9662\u958b\u59cb\u4e86\u5168\u7403\u7b2c\u4e00\u8d77\u4ee5 CRISPR \u70ba\u6cbb\u7642\u4e3b\u8ef8\u7684\u81e8\u5e8a\u8a66\u9a57\u3002\u76e7\u923e\u535a\u58eb\u4ee5\u88ab\u8a3a\u65b7\u51fa\u7f79\u6709\u975e\u5c0f\u7d30\u80de\u80ba\u764c\uff0c\u4e14\u767c\u73fe\u816b\u7624\u5df2\u8f49\u79fb\u7684\u60a3\u8005\u70ba\u53d7\u8a66\u8005\u3002\u5728\u4ed6\u5011\u7684\u6cbb\u7642\u7b56\u7565\u88e1\uff0c\u6253\u7b97\u5229\u7528 CRISPR \u6280\u8853\uff0c\u5728\u5be6\u9a57\u5ba4\u88e1\u5c07 PD-1 \u57fa\u56e0\u5f9e\u53d7\u8a66\u8005\u81ea\u8eab\u7684T\u7d30\u80de\u5167\u5254\u9664\u6389\uff0c\u7136\u5f8c\u518d\u5c07 PD-1 \u57fa\u56e0\u5254\u9664\u7684T\u7d30\u80de\u57f9\u990a\u5230\u4e00\u5b9a\u6578\u91cf\u4e4b\u5f8c\uff0c\u91cd\u65b0\u6ce8\u5c04\u56de\u60a3\u8005\u9ad4\u5167\u3002<\/p>\n

\u70ba\u4ec0\u9ebc\u662f\u5c07\u7126\u9ede\u653e\u5728 PD-1 \u5462\uff1f\u514d\u75ab\u7cfb\u7d71\u8981\u80fd\u597d\u597d\u767c\u63ee\u6240\u9577\u7684\u6700\u4e3b\u8981\u5b88\u5247\uff0c\u5c31\u662f\u80fd\u5206\u8fa8\u300c\u6575\u300d\u3001\u300c\u6211\u300d\uff0c\u4ee5\u514d\u5728\u62b5\u6297\u5165\u4fb5\u75c5\u539f\u83cc\u7684\u6642\u5019\uff0c\u8aa4\u64ca\u81ea\u8eab\u7d30\u80de\u800c\u5c0e\u81f4\u81ea\u9ad4\u514d\u75ab\u53cd\u61c9\u3002\u800c\u70ba\u4e86\u5fb9\u5e95\u57f7\u884c\u9019\u689d\u5927\u539f\u5247\uff0c\u514d\u75ab\u53cd\u61c9\u4f48\u4e0b\u8a31\u591a\u7684\u300c\u6aa2\u67e5\u7ad9\u300d\uff08checkpoints\uff09\uff0c\u800c PD-1 \u86cb\u767d\u8cea\u5c31\u662f\u5176\u4e2d\u4e4b\u4e00\u3002PD-1 \u86cb\u767d\u8cea\u50cf\u662fT\u7d30\u80de\u7684\u715e\u8eca\u4e00\u6a23\uff1b\u56e0\u70ba\u6211\u5011\u81ea\u8eab\u7d30\u80de\u90fd\u6703\u8868\u73fe PD-1 \u7684\u53d7\u8cea PD-L1\uff0c\u7576 PD-1 \u548c\u5176\u53d7\u9ad4 PD-L1 \u7d50\u5408\u4e4b\u5f8c\uff0c\u555f\u52d5\u7684\u8a0a\u606f\u50b3\u905e\u6703\u544a\u8a34 T \u7d30\u80de\uff1a\u300c\u9019\u662f\u81ea\u5df1\u4eba\u5566\uff01\u300d\uff0c\u800c\u6291\u5236 T \u7d30\u80de\u514d\u75ab\u53cd\u61c9\u3002\u8a31\u591a\u7684\u816b\u7624\u7d30\u80de\uff0c\u4e5f\u5c31\u4f9d\u6a23\u756b\u846b\u8606\u5730\u8868\u73fe\u5927\u91cf\u7684PD-L1\uff0c\u4f01\u5716\u4ee5\u62ab\u8457\u7f8a\u76ae\u7684\u72fc\u4e4b\u59ff\u77c7\u9a19\u904e\u95dc\uff0c\u597d\u8eb2\u904eT\u7d30\u80de\u7684\u653b\u64ca\u3002\u56e0\u6b64\uff0c\u8a31\u591a\u81e8\u5e8a\u8a66\u9a57\u90fd\u4ee5\u5229\u7528\u6297\u9ad4\u4f86\u963b\u65b7 PD-1 \u6216\u662f PD-L1 \u6240\u50b3\u905e\u7684\u6291\u5236\u8a0a\u606f\u70ba\u5fd7\uff0c\u4e5f\u90fd\u5c62\u5c62\u6709\u597d\u6d88\u606f\u65ac\u7372\u3002\u4ee5\u963b\u65b7 PD-1 \u8a0a\u606f\u6291\u5236\u5291\u70ba\u4f8b\uff0c\u5169\u682a\u6297\u9ad4\u85e5 Pembrolizumab \u548c Nivolumab\uff0c\u5728\u6cbb\u7642\u975e\u5c0f\u7d30\u80de\u80ba\u764c\u3001\u76ae\u819a\u764c\u4e2d\u7684\u9ed1\u8272\u7d20\u7624\uff0c\u8207\u5176\u4ed6\u60e1\u6027\u816b\u7624\u4e0a\uff0c\u90fd\u5df2\u6709\u986f\u8457\u6210\u6548\u3002<\/p>\n

\u65e2\u7136\u5df2\u77e5\u963b\u65b7 PD-1 \u5c0d\u65bc\u6cbb\u7642\u975e\u5c0f\u7d30\u80de\u80ba\u764c\u662f\u6210\u529f\u7684\u7b56\u7565\uff0c\u76e7\u923e\u535a\u58eb\u6240\u4e3b\u5c0e\u7684 CRISPR \u81e8\u5e8a\u8a66\u9a57\uff0c\u81ea\u7136\u5c31\u8b93\u4eba\u5f88\u671f\u5f85\u6700\u7d42\u7d50\u679c\u3002\u7406\u8ad6\u4e0a\uff0c\u5229\u7528 CRISPR \u6280\u8853\u5254\u9664\u6389T\u7d30\u80de\u5167\u7684 PD-1 \u57fa\u56e0\uff0c\u6703\u8b93 T \u7d30\u80de\u6bba\u622e\u816b\u7624\u7d30\u80de\u7684\u529f\u529b\u5927\u589e\u3002\u800c\u4e14\uff0c\u56e0\u70ba\u662f\u4f7f\u7528\u60a3\u8005\u81ea\u5df1\u7684 T \u7d30\u80de\uff0c\u4e5f\u5c31\u4e0d\u7528\u64d4\u5fc3\u6703\u6709\u79fb\u690d\u6392\u65a5\u7684\u526f\u4f5c\u7528\uff0c\u9019\u4e5f\u662f CRISPR \u6cbb\u7642\u8b93\u79d1\u5b78\u5bb6\u773c\u775b\u767c\u4eae\u7684\u91cd\u9ede\u4e4b\u4e00\u3002<\/p>\n

\u76e7\u923e\u535a\u58eb\u8a08\u756b\u89c0\u5bdf\u60a3\u8005\u534a\u5e74\uff0c\u4ee5\u76e3\u6e2cCRISPR\u6cbb\u7642\u7b56\u7565\u5728\u81e8\u5e8a\u4e0a\u7684\u5b89\u5168\u6027\u70ba\u4e3b\u8981\u8003\u91cf\uff1b\u540c\u6642\uff0c\u4ed6\u4e5f\u5c07\u53d7\u8a66\u8005\u5206\u70ba\u63a5\u53d7\u5169\u500b\u6cbb\u7642\u5468\u671f\u3001\u8207\u4e09\u500b\u3001\u56db\u500b\u6cbb\u7642\u9031\u671f\u4e09\u7d44\uff0c\u85c9\u6a5f\u63a2\u8a0e\u6ce8\u5c04\u56de\u60a3\u8005\u9ad4\u5167\u7684T\u7d30\u80de\u300c\u91cf\u300d\uff0c\u662f\u4e0d\u662f\u548c\u6cbb\u7642\u6210\u6548\u6709\u6b63\u76f8\u95dc\u3002\u64da\u805e\uff0c\u7b2c\u4e00\u500b\u53d7\u8a66\u8005\u5df2\u57282016\u5e74\u5341\u6708\u5e95\u63a5\u53d7\u4e86\u7b2c\u4e00\u5291\u6ce8\u5c04\uff0c\u81f3\u65bc\u6210\u6548\u5982\u4f55\uff0c\u61c9\u8a72\u53ef\u4ee5\u8aaa\u662f\u6574\u500b\u4e16\u754c\u90fd\u5728\u5f15\u9838\u671f\u5f85\u3002<\/p>\n

\u4f46\uff0c\u8eab\u70ba CRISPR \u767c\u6e90\u5730\u7684\u7f8e\u570b\uff0c\u81ea\u7136\u4e0d\u6703\u8b93\u4e2d\u570b\u5c08\u7f8e\u65bc\u524d\u3002\u76ee\u524d\uff0c\u7531\u7f8e\u570b\u8cd3\u5dde\u5927\u5b78\uff08UPenn\uff09\u3001\u52a0\u5dde\u5927\u5b78\u820a\u91d1\u5c71\u5206\u6821\uff08UCSF\uff09\uff0c\u8207\u5fb7\u5dde\u5927\u5b78\u5b89\u5fb7\u68ee\u764c\u75c7\u4e2d\u5fc3\uff08MD Anderson Cancer Center\uff09\u5408\u4f5c\u7684\u81e8\u5e8a\u8a66\u9a57\u7b2c\u4e00\u671f\u8a08\u756b\uff0c\u7b49\u7f8e\u570b\u98df\u54c1\u85e5\u7269\u76e3\u7763\u7ba1\u7406\u5c40\uff08FDA\uff09\u5be9\u6838\u901a\u904e\u5176\u8a66\u9a57\u7528\u65b0\u85e5\u7684\u7533\u8acb\u4e4b\u5f8c\uff0c\u6253\u7b97\u57282017\u5e74\u4e0a\u534a\u5e74\u9cf4\u69cd\u8d77\u8dd1\u3002<\/p>\n

\u4e0d\u540c\u65bc\u4e2d\u570b\u76e7\u923e\u535a\u58eb\u7684\u81e8\u5e8a\u8a66\u9a57\uff0c\u7f8e\u570b\u9019\u4e09\u5b78\u8853\u5de8\u982d\u6253\u7b97\u5c07\u77db\u982d\u5c0d\u6e96\u591a\u767c\u6027\u9aa8\u9ad3\u7624\uff08multiple myeloma\uff09\u3001\u9ed1\u8272\u7d20\u7624\uff08melanoma\uff09\uff0c\u8207\u8089\u7624\uff08sarcoma\uff09\u3002\u9664\u4e86\u5229\u7528 CRISPR \u6280\u8853\u5c07\u60a3\u8005\u81ea\u8eab T \u7d30\u80de\u7684 PD-1 \u57fa\u56e0\u5254\u9664\u6389\uff0c\u7814\u7a76\u4eba\u54e1\u9084\u6253\u7b97\u4e00\u4f75\u522a\u6389\u7d30\u80de\u4e0a\u7684 T \u7d30\u80de\u6297\u539f\u53d7\u9ad4\uff08T cell receptor\uff0c\u4e5f\u5c31\u662f\u5e38\u898b\u7684 TCR\uff09\u3002T \u7d30\u80de\u6297\u539f\u53d7\u9ad4\uff08TCR\uff09\u53ef\u80fd\u662fT\u7d30\u80de\u4e0a\u6700\u91cd\u8981\u7684\u53d7\u9ad4\uff0c\u6c7a\u5b9a\u8457 T \u7d30\u80de\u6240\u80fd\u5920\u8fa8\u8a8d\u7684\u6297\u539f\u5c08\u4e00\u6027\u662f\u4f55\u65b9\u795e\u8056\u3002\u56e0\u6b64\uff0c\u5982\u679c T \u7d30\u80de\u7fa4\u4e0a\u7684 T \u7d30\u80de\u6297\u539f\u53d7\u9ad4\u901a\u901a\u5254\u9664\u6389\uff0c\u5c31\u50cf\u662f\u628a\u539f\u4f86\u80fd\u8fa8\u8a8d\u5305\u7f85\u842c\u8c61\u5343\u5947\u767e\u602a\u75c5\u539f\u83cc\u6297\u539f\u7684 T \u7d30\u80de\uff0c\u5c08\u4e00\u6027\u901a\u901a\u6b78\u96f6\uff0c\u8b93\u7814\u7a76\u4eba\u54e1\u53ef\u4ee5\u505a\u4e00\u4ef6\u8d85\u7d1a\u9177\u7684\u4e8b\uff1a\u628a\u80fd\u5920\u8fa8\u8a8d\u300c\u816b\u7624\u6297\u539f\u300d\u7684 T \u7d30\u80de\u6297\u539f\u53d7\u9ad4\uff0c\u72f8\u8c93\u63db\u592a\u5b50\u822c\u7684\u653e\u56de\u53bb\u3002<\/p>\n

\u800c\u5728\u7f8e\u570b\u9019\u500b\u81e8\u5e8a\u8a66\u9a57\u88e1\uff0c\u7814\u7a76\u4eba\u54e1\u6253\u7b97\u628a\u80fd\u5920\u8fa8\u8a8d\u816b\u7624\u6297\u539f\u300cNY-ESO-1\u300d\u7684T\u7d30\u80de\u6297\u539f\u53d7\u9ad4\u653e\u56de\u60a3\u8005\u81ea\u8eab\u7684T\u7d30\u80de\u5167\u3002\u52a0\u4e0a\u9019\u4e9b\u88ab\u6539\u9020\u7684T\u7d30\u80de\u4e0d\u518d\u80fd\u5920\u8868\u73fe PD-1\uff0c\u5c31\u7b49\u65bc\u662f\u5728\u8d85\u7d1a\u6230\u58eb\u88dd\u4e86 GPS \u5b9a\u4f4d\u7cfb\u7d71\uff0c\u80fd\u5920\u6e96\u78ba\u9059\u63a7\u9019\u4e9b\u8d85\u7d1a\u6230\u58eb\u627e\u5230\u816b\u7624\u7d30\u80de\u7684\u96b1\u533f\u8655\uff0c\u597d\u5c07\u816b\u7624\u7d30\u80de\u901a\u901a\u6bb2\u6ec5\uff0c\u662f\u56f0\u96e3\u5ea6\u9ad8\u3001\u4f46\u4e5f\u66f4\u6709\u6230\u7565\u610f\u7fa9\u7684\u81e8\u5e8a\u8a66\u9a57\u3002<\/p>\n

\u9664\u4e86\u764c\u75c7\uff0c\u8a31\u591a\u5148\u5929\u907a\u50b3\u6027\u75be\u75c5\u4e5f\u662f\u85e5\u5ee0\u8207\u751f\u6280\u516c\u53f8\u8e8d\u8e8d\u6b32\u8a66\u7684\u7bc4\u7587\u3002\u50cf\u662f\u942e\u5200\u578b\u8ca7\u8840\uff08sickle cell diseases\uff09\u3001\u907a\u50b3\u5148\u5929\u6027\u8996\u7db2\u819c\u5931\u990a\u75c7\u4e2d\u7684\u840a\u4f2f\u6c0f\u5148\u5929\u6027\u9ed1\u77c7\u75c7\uff08Leber congenital amaurosis\uff09\uff0c\u8207\u7531\u8f49\u7532\u72c0\u817a\u86cb\u767d\u8b8a\u7570\u6240\u5c0e\u81f4\u7684\u6fb1\u7c89\u6a23\u86cb\u767d\u75be\u75c5\uff08transthyretin amyloidosis\uff09\uff0c\u4e5f\u90fd\u5206\u5225\u6709\u81e8\u5e8a\u8a66\u9a57\u7684\u8a66\u9a57\u7528\u65b0\u85e5\u7533\u8acb\u6848\uff0c\u5728\u7b49\u7f8e\u570b\u98df\u54c1\u85e5\u7269\u76e3\u7763\u7ba1\u7406\u5c40\u901a\u904e\u4e4b\u5f8c\uff0c\u5c31\u80fd\u63a8\u884c\u4e86\u5462\uff01\u8b93\u6211\u5011\u4e00\u8d77\u671f\u5f85 CRISPR \u6240\u8a31\u8afe\u7684\u57fa\u56e0\u6cbb\u7642\u65b0\u7d00\u5143\uff0c\u771f\u7684\u5982\u9810\u671f\u4e2d\u822c\u5a01\u529b\u7121\u7aae\u5427\uff01<\/p>\n

 <\/p>\n

\u53c3\u8003\u8cc7\u6599\uff1a<\/strong><\/p>\n

    \n
  1. You Lu. PD-1 knockout engineered T cells for metastatic non-small lung cancer.<\/a> ClinicalTrials.gov.<\/li>\n
  2. Sheridan C. CRISPR therapeutics push into human testing. Nat Biotechnol. 2017 Jan 10;35(1):3-5. doi: 10.1038\/nbt0117-3.<\/li>\n
  3. Cyranoski D. CRISPR gene-editing tested in a person for the first time. Nature. 2016 Nov 24;539(7630):479. doi: 10.1038\/nature.2016.20988.<\/li>\n
  4. First-in-human CRISPR trial. Nat Biotechnol. 2016 Aug 9;34(8):796. doi: 10.1038\/nbt0816-796a. PMID: 27504762<\/li>\n
  5. Cyranoski D. Chinese scientists to pioneer first human CRISPR trial. Nature. 2016 Jul 28;535(7613):476-7. doi: 10.1038\/nature.2016.20302. PMID: 27466105<\/li>\n
  6. Ledford H. CRISPR: gene editing is just the beginning. Nature. 2016 Mar 10;531(7593):156-9. doi: 10.1038\/531156a. PMID: 26961639<\/li>\n
  7. Dever DP, et al. CRISPR\/Cas9 \u03b2-globin gene targeting in human haematopoietic stem cells. Nature. 2016 Nov 17;539(7629):384-389. doi: 10.1038\/nature20134.<\/li>\n
  8. Rapoport AP, et al. NY-ESO-1-specific TCR-engineered T cells mediate sustained antigen-specific antitumor effects in myeloma. Nat Med. 2015 Aug;21(8):914-21. doi: 10.1038\/nm.3910.<\/li>\n
  9. DeWitt MA, et al. Selection-free genome editing of the sickle mutation in human adult hematopoietic stem\/progenitor cells. Sci Transl Med. 2016 Oct 12;8(360):360ra134. PMID: 27733558<\/li>\n<\/ol>\n

    --<\/strong>
    \n\u4f5c\u8005\uff1a\u99f1\u5b9b\u7433\u3000\u7f8e\u570b\u8056\u8def\u6613\u83ef\u76db\u9813\u5927\u5b78\uff08Washington University in St. Louis\uff09\u514d\u75ab\u5b78\u535a\u58eb\uff0c\u5f9e\u4e8bT\u7d30\u80de\u767c\u80b2\u8207\u6d3b\u5316\u76f8\u95dc\u7684\u8a0a\u606f\u50b3\u5c0e\u7814\u7a76\u3002<\/strong><\/span><\/p>\n

     <\/p>\n

    \"\u52a0\u5165\u597d\u53cb\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    \u25a0 CRISPR \u6240\u5e36\u4f86\u7684\u57fa\u56e0\u6cbb\u7642\u9858\u666f\u5982\u6b64\u8b93\u4eba\u671f\u5f85\uff0c\u54ea\u4e9b\u75be\u75c5\u6703\u662f\u8b93\u79d1\u5b78\u5bb6\u8e8d\u8e8d\u6b32\u8a66\u7684\u5e78\u904b\u5152\uff0c\u6210\u70ba\u9019\u5283\u6642\u4ee3\u91ab\u7642\u65b0\u7b56\u7565\u7684\u6572\u9580\u78da\u5462\uff1f<\/p>\n","protected":false},"author":20,"featured_media":26652,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,1025],"tags":[3075,3108],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2016\/12\/CRISPR-2.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/27124"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27124"}],"version-history":[{"count":4,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/27124\/revisions"}],"predecessor-version":[{"id":31700,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/27124\/revisions\/31700"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/26652"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27124"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27124"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}