{"id":21988,"date":"2015-08-07T00:35:25","date_gmt":"2015-08-06T16:35:25","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=21988"},"modified":"2018-08-01T16:01:43","modified_gmt":"2018-08-01T08:01:43","slug":"%e3%80%90%e6%a4%8d%e7%89%a9%e7%a0%94%e7%a9%b6%e3%80%91%e5%9c%a8%e5%ae%b6%e5%90%88%e6%88%90%e9%b4%89%e7%89%87%e7%9a%84%e6%9c%80%e5%be%8c%e4%b8%80%e5%93%a9%ef%bc%8c%e9%81%94%e6%88%90%ef%bc%81","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=21988","title":{"rendered":"\u3010\u690d\u7269\u7814\u7a76\u3011\u5728\u5bb6\u5408\u6210\u9d09\u7247\u7684\u6700\u5f8c\u4e00\u54e9\uff0c\u9054\u6210\uff01"},"content":{"rendered":"
\"\u9d09\u7247\u7f4c\u7c9f\u3002\u5716\u7247\u4f86\u6e90\uff1awiki\"
\u9d09\u7247\u7f4c\u7c9f\u3002\u5716\u7247\u4f86\u6e90\uff1awiki<\/figcaption><\/figure>\n

\u64b0\u6587\uff5c<\/strong>\u8449\u7da0\u8212<\/p>\n

\u4e0a\u6b21\u6211\u5011\u63d0\u5230\u5169\u500b\u5718\u968a\u5df2\u7d93\u5e7e\u4e4e\u628a\u5408\u6210\u9d09\u7247\u6240\u9700\u7684\u9175\u7d20\u653e\u5230\u7d30\u83cc\u8207\u9175\u6bcd\u83cc\u4e2d\uff08\u898b<\u5728\u5bb6\u5408\u6210\u9d09\u7247\u53ef\u80fd\u55ce\uff1f<\/a>> \uff09\uff0c\u6700\u8fd1\u767c\u8868\u5728Sciencexpress<\/em>\u7684\u7814\u7a76\u8ad6\u6587\uff0c\u66f4\u5df2\u7d93\u7a81\u7834\u4e86\u9019\u4e00\u9ede\uff01<\/p>\n

\u5148\u524d\u7684\u7814\u7a76\u5df2\u7d93\u9032\u5c55\u5230\uff0c\u53ef\u4ee5\u7531(R,S)<\/em>-Norlaudanosoline\u5408\u6210\u5230(S)<\/em>-reticuline\uff1b\u800c(R)<\/em>-reticuline\u5230\u55ce\u5561\u4e5f\u5df2\u7d93\u5b8c\u6210\uff0c\u5c31\u7368\u7368\u5f9e(S)<\/em>-reticuline\u5230(R)<\/em>-reticuline\u6c92\u8fa6\u6cd5\u3002<\/p>\n

\u52a0\u62ff\u5927\u7684\u7814\u7a76\u5718\u968a\u7576\u521d\u63a8\u60f3\u8a8d\u70ba\uff1a\u7531(S)<\/em>-reticuline\u5230(R)<\/em>-reticuline\u9700\u8981\u4e09\u500b\u9175\u7d20\uff1b\u4e0d\u904e\uff0c\u4e5f\u6709\u5176\u4ed6\u79d1\u5b78\u5bb6\u8a8d\u70ba\u53ea\u9700\u8981\u5169\u500b\u9175\u7d20\u3002\u5230\u5e95\u662f\u4e09\u500b\u9084\u662f\u5169\u500b\u5462\uff1f\u6700\u8fd1\uff0c\u9019\u500b\u554f\u984c\u88ab\u7531\u82f1\u570b\u8207\u6fb3\u6d32\u7d44\u6210\u7684\u7814\u7a76\u5718\u968a\u89e3\u7b54\u4e86\uff01\u539f\u4f86\u5f9e(S)<\/em>-reticuline\u5230(R)<\/em>-reticuline\u6240\u9700\u8981\u7684\u9175\u7d20\u53ea\u6709\u300c\u4e00\u300d\u500b\uff08\u53c31\uff09\uff01<\/p>\n

\u4e0d\u904e\uff0c\u9019\u4e00\u500b\u9175\u7d20\u5176\u5be6\u540c\u6642\u5177\u5099\u6709\u7d30\u80de\u8272\u7d20P450(cytochrome P450)\u8207\u6c27\u5316\u9084\u539f\u9176(oxidoreductase)\u6d3b\u6027\uff0c\u6240\u4ee5\u53ef\u4ee5\u8aaa\u539f\u5148\u63a8\u60f3\u8a8d\u70ba\u662f\u5169\u500b\u9175\u7d20\u7684\u79d1\u5b78\u5bb6\uff0c\u4e5f\u6c92\u731c\u932f\u3002<\/p>\n

\u70ba\u4ec0\u9ebc\u82f1\u570b\u8207\u6fb3\u6d32\u7684\u7814\u7a76\u5718\u968a\u9019\u9ebc\u53b2\u5bb3\uff0c\u53ef\u4ee5\u627e\u5230\u52a0\u62ff\u5927\u5718\u968a\u627e\u4e0d\u5230\u7684\u57fa\u56e0\u5462\uff1f\u539f\u4f86\u4ed6\u5011\u624b\u4e0a\u6709\u4e00\u500b\u7a81\u8b8a\u7a2e\u9d09\u7247\u7f4c\u7c9f\u3002\u9019\u500b\u7a81\u8b8a\u7a2e\u9ad4\u5167\u7684reticuline\u6fc3\u5ea6\u7279\u5225\u9ad8\uff0c\u56e0\u70ba\u5b83\u7121\u6cd5\u628a(S)<\/em>-reticuline\u8f49\u70ba(R)<\/em>-reticuline\u3002<\/p>\n

\u4f46\u662f\uff0c\u9019\u500b\u7a81\u8b8a\u7a2e\u904e\u53bb\u5c31\u5df2\u7d93\u6709\u4e86\u3002\u53ea\u662f\uff0c\u7576\u6642\u7814\u7a76\u5b83\u7684\u4eba\u4e26\u4e0d\u8a8d\u70ba\u5b83\u662f\u5c11\u4e86\u628a(S)<\/em>-reticuline\u8f49\u70ba(R)<\/em>-reticuline\u7684\u80fd\u529b\uff1b\u56e0\u70ba\u7576\u6642\u4ed6\u5011\u4f7f\u7528\u4e86\u5e72\u64feRNA\u7684\u6280\u8853\u88fd\u9020\u4e86\u7a81\u8b8a\u7a2e\uff0c\u4f7f\u5b83\u7522\u751f\u4e86codeinone reductase\u9019\u500b\u57fa\u56e0\u7684\u7f3a\u5931\u3002\u5947\u5999\u7684\u662f\uff0c\u96d6\u7136\u9019\u500b\u57fa\u56e0\u4f4d\u65bc\u6574\u689d\u4ee3\u8b1d\u9014\u5f91\u7684\u4e0b\u6e38\uff0c\u5b83\u7684\u7f3a\u5931\u537b\u4f7f\u5f97(S)<\/em>-reticuline\u7d2f\u7a4d\uff08\u53c32\uff09\uff0c\u4e2d\u9593\u76f8\u5dee\u4e86\u516b\u500b\u6b65\u9a5f\u3002<\/p>\n

\u7576\u6642\u4ed6\u5011\u8a8d\u70ba\uff0c\u9019\u500b\u73fe\u8c61\u61c9\u8a72\u662f\u56de\u994b\u6291\u5236(feedback inhibition)\u7684\u7de3\u6545\uff1b\u4e0d\u904e\u7576\u6642\u4ed6\u5011\u4e5f\u767c\u73fe\uff0c\u4e2d\u9593\u7684\u5e7e\u500b\u9175\u7d20\u8868\u73fe\u91cf\u90fd\u5f88\u6b63\u5e38\u3002\u6709\u9451\u65bc\u6b64\uff0c\u82f1\u570b\u8207\u6fb3\u6d32\u7684\u7814\u7a76\u5718\u968a\u8a8d\u70ba\uff0c\u4e00\u5b9a\u9084\u6709\u5176\u4ed6\u7684\u9175\u7d20\u6709\u554f\u984c\u3002<\/p>\n

\u9032\u4e00\u6b65\u7814\u7a76\u5f8c\u767c\u73fe\uff0c\u539f\u4f86\u9019\u500b\u7a81\u8b8a\u7a2e\u9084\u5c11\u4e86\u53e6\u4e00\u500b\u57fa\u56e0\u300cSTORR<\/em>\u300d\uff0c\u800c\u9019\u500b\u57fa\u56e0\u662f\u4e00\u500b\u540c\u6642\u5177\u5099\u6709\u7d30\u80de\u8272\u7d20P450\u8207\u6c27\u5316\u9084\u539f\u9176\u6d3b\u6027\u7684\u86cb\u767d\u8cea\u3002\u53ea\u8981\u6709\u5b83\u5728\uff0c\u9d09\u7247\u5408\u6210\u7684\u6700\u5f8c\u4e00\u54e9\u5c31\u5b8c\u6210\u4e86\u3002<\/p>\n

STORR\u86cb\u767d\u53ef\u4ee5\u628a(S)<\/em>-reticuline\u5148\u6c27\u5316\u7522\u751f1,2-dehydroreticuline\uff0c\u7136\u5f8c\u518d\u9084\u539f\u751f\u6210(R)<\/em>-reticuline\u3002\u63a5\u8457\u5c31\u4ea4\u7d66\u4ee5\u524d\u7684\u5718\u968a\u56c9~<\/p>\n

\u9019\u4e0b\uff0c\u8981\u5982\u4f55\u898f\u7bc4\u300c\u5728\u5bb6\u91c0\u9d09\u7247\u300d\uff1f\u653f\u5e9c\u55ae\u4f4d\u6709\u5f97\u982d\u75db\u4e86...<\/p>\n

\u53c3\u8003\u6587\u737b\uff1a
\n<\/strong>1. Ian A. Graham et. al., 2015.\u00a0Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein<\/a>. Sciencexpress.
\n2. Philip J Larkin et. al., 2004.\u00a0RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy<\/a>. Nature Biotechnology. 22\uff0812\uff09\uff1a1559- 1566. doi:10.1038\/nbt1033<\/p>\n

--<\/strong><\/span>
\u4f5c\u8005\uff1a\u8449\u7da0\u8212\u3000\u6148\u6fdf\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u52a9\u7406\u6559\u6388\uff0c\u79d1\u6559\u4e2d\u5fc3\u7279\u7d04\u5beb\u624b\uff0c\u5f9e\u4e8b\u79d1\u666e\u6587\u7ae0\u5beb\u4f5c\u3002<\/strong><\/span><\/p>\n

 <\/p>\n

\"\u52a0\u5165\u597d\u53cb\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u64b0\u6587\uff5c\u8449\u7da0\u8212 \u4e0a\u6b21\u6211\u5011\u63d0\u5230\u5169\u500b\u5718\u968a\u5df2\u7d93\u5e7e<\/p>\n","protected":false},"author":20,"featured_media":21989,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,26],"tags":[2018,1953,1543,1952],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/07\/Poppies_in_the_Sunset_on_Lake_Geneva.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/21988"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21988"}],"version-history":[{"count":8,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/21988\/revisions"}],"predecessor-version":[{"id":32316,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/21988\/revisions\/32316"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/21989"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21988"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21988"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21988"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}