{"id":21295,"date":"2015-05-12T00:58:58","date_gmt":"2015-05-11T16:58:58","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=21295"},"modified":"2018-08-01T15:42:17","modified_gmt":"2018-08-01T07:42:17","slug":"%e3%80%90%e7%b9%bd%e7%b4%9b%e7%94%9f%e6%85%8b%e3%80%91%e7%b4%b0%e8%8f%8c%e6%9c%83%e8%a2%ab%e5%99%ac%e8%8f%8c%e9%ab%94%e5%9a%87%e6%ad%bb%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=21295","title":{"rendered":"\u3010\u7e7d\u7d1b\u751f\u614b\u3011\u7d30\u83cc\u6703\u88ab\u566c\u83cc\u9ad4\u5687\u6b7b\uff1f"},"content":{"rendered":"<figure id=\"attachment_21296\" aria-describedby=\"caption-attachment-21296\" style=\"width: 289px\" class=\"wp-caption alignleft\"><a href=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/04\/RT8-4.jpg\"><img fetchpriority=\"high\" decoding=\"async\" class=\"size-medium wp-image-21296\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/04\/RT8-4-289x300.jpg\" alt=\"\u6b63\u5728\u88abSTSV1\u566c\u83cc\u9ad4\u611f\u67d3\u7684Sulfolobus\u3002\u5716\u7247\u4f86\u6e90\uff1awiki\" width=\"289\" height=\"300\" srcset=\"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/04\/RT8-4-289x300.jpg 289w, https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/04\/RT8-4.jpg 539w\" sizes=\"(max-width: 289px) 100vw, 289px\" \/><\/a><figcaption id=\"caption-attachment-21296\" class=\"wp-caption-text\">\u6b63\u5728\u88abSTSV1\u566c\u83cc\u9ad4\u611f\u67d3\u7684Sulfolobus\u3002\u5716\u7247\u4f86\u6e90\uff1awiki<\/figcaption><\/figure>\n<p><strong>\u64b0\u6587\uff5c<\/strong>\u8449\u7da0\u8212<\/p>\n<p>\u6211\u7684\u535a\u73ed\u6b72\u6708\u7e3d\u662f\u570d\u7e5e\u8457\u566c\u83cc\u9ad4(bacteriophage)\u8ddf\u5927\u8178\u687f\u83cc(<em>E. coli<\/em>)\u3002\u990a\u83cc\u5c31\u662f\u70ba\u4e86\u8981\u7d66\u566c\u83cc\u9ad4\u5403\u6389\uff01<\/p>\n<p>\u6709\u4e00\u5929\u508d\u665a\uff0c\u7576\u6211\u628a\u5927\u8178\u687f\u83cc\u63a5\u7a2e\u5b8c\uff0c\u6211\u5ffd\u7136\u60f3\u5230\uff1a\u5982\u679c\u5927\u8178\u687f\u83cc\u77e5\u9053\u4ed6\u5011\u660e\u5929\u8981\u88ab\u62ff\u53bb\u9935\u566c\u83cc\u9ad4\uff08\u4e5f\u5c31\u662f\u4ed6\u5011\u660e\u5929\u90fd\u8981\u300c\u809a\u7834\u8178\u6d41\u300d\u800c\u6b7b\uff09\uff0c\u4ed6\u5011\u9084\u6703\u9577\u55ce\uff1f<\/p>\n<p>\u7576\u6211\u628a\u9019\u500b\u60f3\u6cd5\u8ddf\u5be6\u9a57\u5ba4\u7684\u5176\u4ed6\u4eba\u5206\u4eab\u6642\uff0c\u6709\u4eba\u8aaa\uff1a\u4f60\u5feb\u760b\u4e86\uff0c\u8d95\u5feb\u7562\u696d\u5427\uff01<\/p>\n<p>\u4f46\u662f\uff0c\u6700\u8fd1\u4f0a\u5229\u8afe\u5927\u5b78(University of Illinois at Urbana-Champaign)\u5fae\u751f\u7269\u7cfb\u7684\u7814\u7a76\u5718\u968a\u537b\u767c\u73fe\uff1a\u566c\u83cc\u9ad4\u771f\u7684\u53ef\u4ee5\u300c\u5687\u5230\u300d\u5b83\u7684\u5bbf\u4e3b\uff0c\u4f7f\u5bbf\u4e3b\u300c\u66ab\u6642\u505c\u6b62\u547c\u5438\u300d24-48\u5c0f\u6642\uff1b\u4f46\u82e5\u662f\u8d85\u904e48\u5c0f\u6642\u566c\u83cc\u9ad4\u9084\u4e0d\u8d70\u7684\u8a71\uff0c\u5bbf\u4e3b\u5c31\u6703\u6b7b\u7ff9\u7ff9\u4e86\uff01<!--more--><\/p>\n<p>\u5728\u9019\u500b\u7814\u7a76\u88e1\uff0c\u79d1\u5b78\u5bb6\u5011\u4f7f\u7528\u4e86\u53e4\u83cc\u9580(<em>Archea<\/em>)\u7684<em>Sulfolobus islandicus\u00a0<\/em>RJW002\uff08\u4ee5\u4e0b\u7c21\u7a31<em>S. islandicus<\/em>\uff09\u4ee5\u53ca\u5b83\u7684\u566c\u83cc\u9ad4SSV9(<em>Sulfolobus<\/em>\u00a0spindle-shaped virus 9)\u3002\u8aaaSSV9\u662f<em>S. islandicus<\/em>\u7684\u5c08\u5c6c\u566c\u83cc\u9ad4\u53ef\u80fd\u4e0d\u70ba\u904e\uff0c\u7562\u7adf\u4e00\u958b\u59cb\u9019\u96bb\u566c\u83cc\u9ad4\u5c31\u662f\u5f9e\u5b83\u5206\u96e2\u51fa\u4f86\u7684\uff1b\u800c<em>S. islandicus<\/em>\u7684\u514d\u75ab\u7cfb\u7d71CRISPR-Cas\u5177\u6709\u8207SSV9\u767e\u5206\u4e4b\u767e\u76f8\u540c\u7684\u5e8f\u5217\uff0c\u986f\u793a\u4e86\u5b83\u7d93\u5e38\u88abSSV9\u611f\u67d3\u3002<\/p>\n<p>\u770b\u5230\u9019\u88e1\uff0c\u8b80\u8005\u53ef\u80fd\u6703\u60f3\u5230\uff1a\u4ec0\u9ebc\uff1f\u7d30\u83cc\u6709\u514d\u75ab\u7cfb\u7d71\uff1f<\/p>\n<p>\u662f\u7684\u3002\u8a31\u591a\u53e4\u83cc\u9580\u7684\u6210\u54e1\uff0c\u5728\u81ea\u5df1\u7684\u57fa\u56e0\u9ad4\u4e2d\u90fd\u5e36\u4e86\u4e00\u6bb5\u566c\u83cc\u9ad4\u7684\u5e8f\u5217\uff0c\u7528\u4f86\u8fa8\u8a8d\u81ea\u5df1\u662f\u5426\u906d\u5230\u566c\u83cc\u9ad4\u5165\u4fb5\u3002\u4e00\u65e6\u566c\u83cc\u9ad4\u7684\u5e8f\u5217\u5728\u9ad4\u5167\u88ab\u8fa8\u8a8d\u51fa\u4f86\uff0c\u4fbf\u53ef\u4ee5\u5c07\u4e4b\u6e05\u9664\u6389\u3002\u9019\u500b\u7cfb\u7d71\u88ab\u7a31\u70baCRISPR-Cas\u514d\u75ab\u7cfb\u7d71\u3002<\/p>\n<p>\u7814\u7a76\u5718\u968a\u767c\u73fe\uff0c\u5373\u4f7f\u5c07SSV9\u4ee5\u4e00\u6bd4\u4e00\u767e\u7684\u6bd4\u4f8b\u52a0\u5230<em>S. islandicus<\/em>\u4e2d\uff0c\u9019\u6a23\u7a00\u5c11\u7684\u566c\u83cc\u9ad4\uff0c\u4ecd\u820a\u6703\u9020\u6210<em>S. islandicus<\/em>\u7684\u751f\u9577\u5ef6\u7de9\u4e8624-48\u5c0f\u6642\u3002\u540c\u6a23\u7684\u6642\u9593\u88e1\uff0c\u4e0d\u52a0\u5165SSV9\u7684\u7d44\u5225\u5df2\u7d93\u5206\u88c2\u4e86\u5169\u6b21\u4ee5\u4e0a\u3002<\/p>\n<p>\u7576\u4ed6\u5011\u628a\u9019\u4e9b\u83cc\u653e\u5728\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u6642\u767c\u73fe\uff0c\u8207SSV9\u5171\u57f9\u990a24\u5c0f\u6642\u7684\u83cc\uff0c\u670995%\u90fd\u6210\u4e86\u7a7a\u7684\u7d30\u80de\u3002\u4e0d\u904e\uff0c\u96d6\u7136\u9019\u4e9b\u7d30\u80de\u7684\u578b\u614b\u6539\u8b8a\u3001\u5728\u986f\u5fae\u93e1\u4e0b\u770b\u8d77\u4f86\u4e5f\u50cf\u662f\u7a7a\u7684\uff1b\u4f46\u4ee5\u5857\u76e4\u65b9\u5f0f\u4f86\u8a08\u7b97\u83cc\u843d\u6642\u537b\u6c92\u6709\u6e1b\u5c11\u3002\u7531\u65bc\u5857\u76e4\u8a08\u7b97\u9700\u5c07\u83cc\u5857\u5728\u57f9\u990a\u57fa\u4e0a\u9762\uff0c\u4e5f\u5c31\u53bb\u6389\u4e86\u566c\u83cc\u9ad4\uff0c\u56e0\u6b64\u7814\u7a76\u5718\u968a\u8a8d\u70ba\uff1a\u9019\u4e9b\u770b\u8d77\u4f86\u50cf\u7a7a\u7684\u83cc\uff0c\u5176\u5be6\u53ea\u662f\u9032\u5165\u4e86\u4f11\u7720\u72c0\u614b\uff0c\u4e00\u65e6\u53bb\u6389\u4e86\u566c\u83cc\u9ad4\uff0c\u4ed6\u5011\u5c31\u80fd\u5920\u6062\u5fa9\u751f\u6a5f\u3002<\/p>\n<p>\u4f46\u662f\uff0c\u7a76\u7adf<em>S. islandicus<\/em>\u7684\u4f11\u7720\u662f\u56e0\u70ba\u566c\u83cc\u9ad4\u7684\u611f\u67d3\uff0c\u9084\u662f\u53ea\u8981\u6709\u566c\u83cc\u9ad4\u5b58\u5728\u5c31\u53ef\u4ee5\u5f15\u767c\u4f11\u7720\u5462\uff1f\u7814\u7a76\u5718\u968a\u7528\u7d2b\u5916\u5149\u6bba\u6b7bSSV9\uff0c\u518d\u628a\u9019\u4e9b\u6b7b\u6389\u7684\u566c\u83cc\u9ad4\u653e\u9032\u57f9\u990a\u6db2\u4e2d\u3002\u7d50\u679c\u767c\u73fe\u6b7b\u6389\u7684\u566c\u83cc\u9ad4\u4e00\u6a23\u53ef\u4ee5\u9020\u6210\u83cc\u7684\u4f11\u7720\uff0c\u800c\u4e14\u5982\u679c\u4e00\u76f4\u8b93\u9019\u4e9b\u83cc\u8207\u566c\u83cc\u9ad4\u5728\u4e00\u8d77\uff0c\u523072\u5c0f\u6642\u6642\uff0c\u6240\u6709\u7684\u83cc\u5168\u90e8\u90fd\u6b7b\u5149\u5149\u4e86\uff01<\/p>\n<p>\u7576\u7136\uff0c\u4e0d\u80fd\u6392\u9664\u662f\u5426\u56e0\u70ba\u83cc\u7684\u514d\u75ab\u7cfb\u7d71\u6240\u7522\u751f\u7684\u53cd\u61c9\u3002\u4e0d\u904e\uff0c\u7814\u7a76\u5718\u968a\u4e5f\u88fd\u4f5c\u4e86\u4e0d\u5177\u6709\u514d\u75ab\u80fd\u529b\u7684<em>S. islandicus<\/em>\uff0c\u767c\u73fe\u566c\u83cc\u9ad4\u5b8c\u5168\u53ef\u4ee5\u611f\u67d3\u6c92\u6709\u514d\u75ab\u80fd\u529b\u7684\u83cc\uff0c\u7522\u751f\u5f88\u591a\u5f88\u591a\u7684\u566c\u83cc\u9ad4\uff08\u611f\u67d324\u5c0f\u6642\u5f8c\u53ea\u67091%\u7684\u83cc\u5b58\u6d3b\uff09\uff0c\u800c\u9019\u4e9b\u83cc\u5728\u9047\u5230\u6b7b\u6389\u7684\u566c\u83cc\u9ad4\u6642\uff0c\u4e00\u6a23\u6703\u7522\u751f\u4f11\u7720\uff0c\u8d85\u904e48\u5c0f\u6642\u4e5f\u4e00\u6a23\u6703\u6b7b\u5149\u5149\u5594\uff01\u6240\u4ee5\uff0c\u9019\u500b\u4f11\u7720\u53cd\u61c9\u4e26\u4e0d\u662f\u53ea\u6709\u5177\u5099\u6709\u514d\u75ab\u80fd\u529b\u7684\u83cc\u6240\u7279\u6709\uff0c\u800c\u662f\u5927\u5bb6\u6240\u5171\u6709\u7684\uff1b\u4e5f\u8ddf\u88ab\u611f\u67d3\u8207\u5426\u6c92\u6709\u95dc\u4fc2\u5462\uff01<\/p>\n<p>\u7576\u7136\uff0c\u7a76\u7adf\u4f11\u7720\u7684\u76ee\u7684\u662f\u70ba\u4ec0\u9ebc\u5462\uff1f\u6709\u79d1\u5b78\u5bb6\u8a8d\u70ba\uff0c\u4f11\u7720\u662f\u70ba\u4e86\u8b93\u83cc\u80fd\u5920\u5f9e\u566c\u83cc\u9ad4\u8eab\u4e0a\u7372\u53d6\u66f4\u591a\u7684\u8cc7\u8a0a\uff0c\u4f7f\u83cc\u7684\u514d\u75ab\u7cfb\u7d71\u53ef\u4ee5\u5f37\u5316\u81ea\u5df1\uff0c\u4e0b\u6b21\u9047\u5230\u540c\u6a23\u7684\u566c\u83cc\u9ad4\u5c31\u4e0d\u6703\u4e56\u4e56\u7e73\u68b0\u6295\u964d\u4e86\uff1b\u9019\u500b\u60f3\u6cd5\u76f8\u7576\u6709\u8da3\uff0c\u4e0d\u904e\u9084\u6709\u5f85\u8b49\u5be6\u3002\u4f46\u662f\uff0c\u4f11\u7720\u53ef\u4ee5\u4f7f\u514d\u75ab\u7cfb\u7d71\u8d95\u4e0a\u9032\u5ea6\u3001\u6293\u5230\u566c\u83cc\u9ad4\u5012\u662f\u5343\u771f\u842c\u78ba\u7684\uff0c\u5728\u9019\u7bc7\u7814\u7a76\u88e1\u4e5f\u770b\u5230\uff0c\u6709\u514d\u75ab\u80fd\u529b\u7684\u83cc\uff0c\u5728\u52a0\u5165\u566c\u83cc\u9ad4\u5f8c24-48\u5c0f\u6642\u90fd\u6e2c\u4e0d\u5230\u65b0\u566c\u83cc\u9ad4\u7684\u7522\u751f\uff0c\u800c\u6c92\u6709\u514d\u75ab\u80fd\u529b\u7684\u83cc\u5247\u7522\u751f\u4e86\u4e00\u5927\u5806\u7684\u566c\u83cc\u9ad4\u3002\u6240\u4ee5\uff0c\u514d\u75ab\u7cfb\u7d71\u81f3\u5c11\u53ef\u4ee5\u9632\u6b62\u611f\u67d3\u6563\u4f48\uff0c\u53ea\u662f\u82e5\u566c\u83cc\u9ad4\u4e00\u76f4\u5f98\u5f8a\u4e0d\u8d70\uff0c\u9019\u4e9b\u83cc\u5c31\u4f86\u500b\u96c6\u9ad4\u81ea\u6bba\uff0c\u597d\u50cf\u4e5f\u592a\u58ef\u70c8\u4e86\u4e00\u9ede\u554a\uff01<\/p>\n<p><strong>\u53c3\u8003\u6587\u737b\uff1a<\/strong><br \/>\nMaria A. Bautista, Changyi Zhang, Rachel J. Whitaker. 2015.\u00a0<a href=\"http:\/\/mbio.asm.org\/content\/6\/2\/e02565-14.abstract\" target=\"_blank\" rel=\"noopener noreferrer\">Virus-Induced Dormancy in the Archaeon\u00a0<em>Sulfolobus islandicus<\/em><\/a>. mBio. 6(2): e02565-14. doi: 10.1128\/mBio.02565-14<\/p>\n<p><span style=\"color: #333399;\"><strong>--<\/strong><\/span><br style=\"color: #000000;\" \/><span style=\"color: #333399;\"><strong>\u4f5c\u8005\uff1a\u8449\u7da0\u8212\u3000\u6148\u6fdf\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u52a9\u7406\u6559\u6388\uff0c\u79d1\u6559\u4e2d\u5fc3\u7279\u7d04\u5beb\u624b\uff0c\u5f9e\u4e8b\u79d1\u666e\u6587\u7ae0\u5beb\u4f5c\u3002<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/line.me\/R\/ti\/p\/%40fcx6182w\" target=\"_blank\" rel=\"noopener noreferrer\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/scdn.line-apps.com\/n\/line_add_friends\/btn\/zh-Hant.png\" alt=\"\u52a0\u5165\u597d\u53cb\" width=\"212\" height=\"36\" border=\"0\"><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u64b0\u6587\uff5c\u8449\u7da0\u8212 \u6211\u7684\u535a\u73ed\u6b72\u6708\u7e3d\u662f\u570d\u7e5e\u8457\u566c\u83cc<\/p>\n","protected":false},"author":20,"featured_media":21296,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,26],"tags":[1807,1703,214,1543],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/04\/RT8-4.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/21295"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21295"}],"version-history":[{"count":5,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/21295\/revisions"}],"predecessor-version":[{"id":32235,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/21295\/revisions\/32235"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/21296"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21295"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21295"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21295"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}