{"id":20716,"date":"2015-02-18T00:21:02","date_gmt":"2015-02-17T16:21:02","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=20716"},"modified":"2018-08-01T14:43:18","modified_gmt":"2018-08-01T06:43:18","slug":"%e3%80%90%e7%b4%b0%e8%83%9e%e4%b8%96%e7%95%8c%e3%80%91%e7%99%bc%e7%8f%be%e5%9b%9e%e6%92%a5%e8%80%81%e5%8c%96%e6%99%82%e9%90%98%e7%9a%84%e6%96%b0%e6%96%b9%e6%b3%95","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=20716","title":{"rendered":"\u3010\u7d30\u80de\u4e16\u754c\u3011\u767c\u73fe\u56de\u64a5\u8001\u5316\u6642\u9418\u7684\u65b0\u65b9\u6cd5"},"content":{"rendered":"
\"Credit:
Credit: \u00a9 gitanna \/ Fotolia<\/figcaption><\/figure>\n

\u64b0\u6587\uff5c<\/strong>\u8449\u7da0\u8212<\/p>\n

\u8fd4\u8001\u9084\u7ae5\uff0c\u6216\u662f\u9577\u751f\u4e0d\u8001\uff0c\u662f\u8a31\u591a\u4eba\u7684\u5922\u60f3\u3002\u5373\u4f7f\u662f\u79e6\u59cb\u7687\uff0c\u4e5f\u5e0c\u671b\u80fd\u5920\u9577\u751f\u4e0d\u8001\u3002\u800c\u574a\u9593\u90a3\u9ebc\u591a\u7684\u300c\u6297\u8001\u5316\u300d\u79d8\u65b9\uff0c\u4e0d\u4e5f\u8aaa\u660e\u4e86\u73fe\u4ee3\u4eba\u5e0c\u671b\u9752\u6625\u6c38\u99d0\uff1f\u6c92\u6709\u4eba\u559c\u6b61\u300c\u8996\u832b\u832b\uff0c\u9aee\u84bc\u84bc\uff0c\u9f52\u7259\u52d5\u6416\u300d\uff0c\u5373\u4f7f\u90a3\u662f\u4e0d\u5f97\u4e0d\u7136\uff0c\u4e5f\u5e0c\u671b\u80fd\u5920\u76e1\u53ef\u80fd\u5730\u5ef6\u7de9\u3002<\/p>\n

\u56e0\u6b64\uff0c\u8fd1\u4ee3\u79d1\u5b78\u4e5f\u4e00\u76f4\u81f4\u529b\u65bc\u627e\u5230\u8001\u5316\u7684\u95dc\u9375\u3002\u6700\u8fd1\uff0c\u53f2\u4e39\u4f5b\u5927\u5b78\u91ab\u5b78\u4e2d\u5fc3\uff08Stanford University Medical Center\uff09\u767c\u73fe\uff0c\u5c07\u7aef\u7c92\u9176(telomerase)\u7684mRNA(\u4fe1\u606fRNA)\u7d93\u904e\u4fee\u98fe\u5f8c\u6ce8\u5165\u7d30\u80de\uff0c\u53ef\u4ee5\u5ef6\u7de9\u8001\u5316\u3002<\/p>\n

\u7aef\u7c92\u9176\u662f\u5e36\u6709\u6838\u7cd6\u6838\u9178(RNA)\u7684\u9175\u7d20\u3002\u5b83\u7684\u529f\u80fd\u662f\u5728\u67d3\u8272\u9ad4\u672b\u7aef\u52a0\u4e0a\u300cTTAGGG\u300d\u7684\u91cd\u8907\u5e8f\u5217(\u7a31\u70ba\u7aef\u7c92telomere)\u3002\u7aef\u7c92\u9176\u81ea\u5df1\u7684RNA\uff0c\u8207\u67d3\u8272\u9ad4\u672b\u7aef\u7684TTAGGG\u5e8f\u5217\u4e92\u88dc\uff0c\u5728\u67d3\u8272\u9ad4\u672b\u7aef\u52a0\u5165\u66f4\u591a\u7684TTAGGG\u91cd\u8907(\u5982\u4e0b\u5716)\u3002<\/p>\n

\"\u7aef\u7c92\u9176\u4f5c\u7528\u5716\u89e3\u3002\u5716\u7247\u4f86\u6e90\uff1awiki\"<\/a>
\u7aef\u7c92\u9176\u4f5c\u7528\u5716\u89e3\uff0c\u9ede\u958b\u53ef\u653e\u5927\u3002\u5716\u7247\u4f86\u6e90\uff1awiki<\/figcaption><\/figure>\n

\u70ba\u4ec0\u9ebc\u9700\u8981\u7aef\u7c92\u9176\u5728\u67d3\u8272\u9ad4\u672b\u7aef\u52a0\u5165\u91cd\u8907\u7684\u5e8f\u5217\u5462\uff1f\u8aaa\u4f86\u8a71\u9577\uff0c\u539f\u4f86\uff0c\u5408\u6210\u67d3\u8272\u9ad4\u7684\u9175\u7d20--DNA \u805a\u5408\u9176(DNA polymerase)\uff0c\u5728\u5408\u6210\u65b0\u7684DNA\u6642\uff0c\u9700\u8981\u6709\u7bc4\u672c(template)\u8207\u5f15\u5b50(primer)\uff0c\u4e26\u4e14\u4e00\u5b9a\u8981\u75315'\u78f7\u9178\u6839\u5f803'\u6c2b\u6c27\u6839\u7684\u65b9\u5411\uff0c\u4f86\u5408\u6210\u65b0\u7684\u4e00\u80a1\u7684DNA(\u5982\u4e0b\u5716)\u3002<\/p>\n

\"DNA\u805a\u5408\u9176\u5408\u6210\u65b0DNA\u7684\u65b9\u5411\u3002\u88fd\u5716\u8005\uff1a\u8449\u7da0\u8212\"
DNA\u805a\u5408\u9176\u5408\u6210\u65b0DNA\u7684\u65b9\u5411\u3002\u88fd\u5716\u8005\uff1a\u8449\u7da0\u8212<\/figcaption><\/figure>\n

\u4f46\u662f\uff0c\u67d3\u8272\u9ad4\u539f\u4f86\u5c31\u662f\u7531\u5169\u689dDNA(\u53bb\u6c27\u6838\u7cd6\u6838\u9178)5'\u7aef(5'\u78f7\u9178\u6839)\u5c0d3'\u7aef(3'\u6c2b\u6c27\u6839)\u69cb\u6210\u7684(\u7531\u4e0a\u5716\u4e5f\u53ef\u4ee5\u770b\u5230)\uff0c\u9019\u6a23\u7684\u69cb\u9020\u7a31\u70ba\u96d9\u80a1\u53cd\u5e73\u884c\u3002\u96d9\u80a1\u53cd\u5e73\u884c\u7684\u69cb\u9020\uff0c\u4f7f\u5f97\u67d3\u8272\u9ad4\u975e\u5e38\u7a69\u5b9a\uff0c\u4e0d\u5bb9\u6613\u53d7\u5230\u74b0\u5883\u4e2d\u7684\u9178\u8207\u9e7c\u7834\u58de\u800c\u5931\u53bb\u6d3b\u6027\u3002\u4f46\u662f\uff0c\u7576DNA\u805a\u5408\u9176\u5728\u5408\u6210\u65b0\u7684\u67d3\u8272\u9ad4\u6642\uff0c\u7531\u65bc\u67d3\u8272\u9ad4\u96d9\u80a1\u53cd\u5e73\u884c\u7684\u69cb\u9020\uff0c\u4f7f\u5f97\u6253\u958b\u7684\u5169\u80a1\uff0c\u5176\u4e2d\u4e00\u80a1\u7684\u65b9\u5411\u8207DNA\u805a\u5408\u9176\u5408\u6210\u7684\u65b9\u5411\u76f8\u53cd\u3002<\/p>\n

\u5982\u4e0a\u5716\uff0c\u5716\u7247\u4e0b\u65b9\u7684\u90a3\u4e00\u80a1DNA\uff0c\u56e0\u70ba\u65b9\u5411\u5c31\u662f\u5f9e3'\u7aef\u52305'\u7aef\uff0c\u6240\u4ee5DNA\u805a\u5408\u9176\u53ef\u4ee5\u5f9eDNA\u7684\u6700\u524d\u7aef\u958b\u59cb\u8907\u88fd\u5230\u6700\u672b\u7aef\u3002\u4f46\u662f\u5716\u7247\u4e0a\u65b9\u7684\u90a3\u4e00\u80a1DNA(\u7a31\u70ba\u5ef6\u9072\u80a1\uff0clagging strand)\u8981\u600e\u9ebc\u8fa6\u5462\uff1f<\/p>\n

\u7531\u65bcDNA\u805a\u5408\u9176\u4e0d\u53ef\u80fd\u53cd\u5411\u8907\u88fd\uff0c\u7576\u6642\u6709\u4eba\u63d0\u51fa\uff1a\u5ef6\u9072\u80a1DNA\u7684\u8907\u88fd\u65b9\u5f0f\uff0c\u662f\u65b7\u65b7\u7e8c\u7e8c\u7684\u8907\u88fd(\u5982\u4e0b\u5716)\u3002\u800c\u9019\u500b\u5047\u8aaa\uff0c\u7531\u5ca1\u5d0e\u4ee4\u6cbb(Reiji Okazaki)\u57281968\u5e74\u8b49\u5be6\u4e86\u3002<\/p>\n

\"\u5ef6\u9072\u80a1\uff08lagging
\u5ef6\u9072\u80a1\uff08lagging strand\uff09\u7684\u8907\u88fd\u65b9\u5f0f\u3002\u88fd\u5716\uff1a\u8449\u7da0\u8212<\/figcaption><\/figure>\n

\u5ca1\u5d0e\u4ee4\u6cbb\u4ee5\u5de7\u5999\u7684\u5be6\u9a57\uff0c\u5206\u96e2\u51fa\u7247\u6bb5\u7684DNA\uff0c\u8b49\u5be6\u4e86\u9019\u500b\u5047\u8aaa\uff1b\u70ba\u4e86\u7d00\u5ff5\u4ed6\uff0c\u9019\u7247\u6bb5\u7684DNA\u4fbf\u7a31\u70ba\u5ca1\u5d0e\u7247\u6bb5(Okazaki fragment)\u3002<\/p>\n

\u4e0d\u904e\uff0c\u89e3\u6c7a\u4e86\u9019\u500b\u554f\u984c\uff0c\u63a5\u8457\u4f86\u7684\u662f\u4e0b\u4e00\u500b\u554f\u984c\u3002\u7576DNA\u8907\u88fd\u7d42\u4e86\u6642\uff0c\u5982\u679c\u662f\u74b0\u72c0\u7684DNA(\u5982\u7d30\u83cc\u7b49\u539f\u6838\u751f\u7269)\uff0c\u6700\u5f8c\u7559\u4e0b\u7684\u7f3a\u53e3\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u88dc\u4e0a\uff1b\u4f46\u7dda\u72c0DNA(\u5982\u6211\u7b49\u771f\u6838\u751f\u7269)\u7684\u8907\u88fd\uff0c\u5230\u4e86DNA\u7684\u6700\u672b\u7aef\u6642\uff0c\u6703\u56e0\u70ba\u7121\u6cd5\u7522\u751f\u5f15\u5b50(\u5c0d\uff0c\u5f15\u5b50\u4e5f\u662f\u7531\u7279\u6b8a\u7684\u9176\u5408\u6210\u7684)\uff0c\u9020\u6210\u5408\u6210\u7684DNA\u300c\u9577\u77ed\u8173\u300d(\u5982\u4e0b\u5716)\u3002<\/p>\n

\"\u7dda\u72c0DNA\u8907\u88fd\u7684\u6700\u7d42\u7522\u7269\u3002\u88fd\u5716\uff1a\u8449\u7da0\u8212\"
\u7dda\u72c0DNA\u8907\u88fd\u7684\u6700\u7d42\u7522\u7269\u3002\u88fd\u5716\uff1a\u8449\u7da0\u8212<\/figcaption><\/figure>\n

\u9019\u500b\u5047\u8aaa\uff0c\u57281973\u5e74\u7531\u6b50\u6d1b\u5c3c\u53ef\u592b(Alexey Olovnikov)\u63d0\u51fa\u3002\u7576\u6642\uff0c\u4ed6\u4e5f\u63d0\u51fa\u751f\u7269\u4e00\u5b9a\u6709\u76f8\u5c0d\u61c9\u7684\u6a5f\u5236\u4f86\u5f4c\u88dc\u9019\u500b\u9577\u77ed\u8173\u7684\u554f\u984c\u3002\u800cCarol W. Greider\uff0c Elizabeth Blackburn \u8207 Jack W. Szostak\u5728 1984\u5e74\u8b49\u5be6\u4e86\u7aef\u7c92\u9176\u7684\u5b58\u5728\u3002<\/p>\n

\u7aef\u7c92\u9176\u5728\u67d3\u8272\u9ad4\u672b\u7aef\u52a0\u4e0a\u300cTTAGGG\u300d\u91cd\u8907\u7684\u5e8f\u5217\uff0c\u65bc\u662f\u5728\u8907\u88fd\u6642\u7684\u300c\u9577\u77ed\u8173\u300d\u554f\u984c\u4fbf\u4e0d\u90a3\u9ebc\u4ee4\u4eba\u64d4\u5fc3\u4e86\u3002\u7562\u7adf\uff0c\u5931\u53bb\u7684\u53ea\u662f\u91cd\u8907\u7684\u5e8f\u5217\uff0c\u800c\u4e14\uff0c\u9084\u6709\u7aef\u7c92\u9176\u4f86\u88dc\u5145\u3002<\/p>\n

\u4f46\u662f\uff0c\u5373\u4fbf\u6709\u7aef\u7c92\u9176\uff0c\u79d1\u5b78\u5bb6\u5011\u767c\u73fe\uff0c\u96a8\u8457\u7d30\u80de\u4e00\u6b21\u6b21\u7684\u5206\u88c2\uff0c\u67d3\u8272\u9ad4\u4e00\u6b21\u6b21\u5730\u8907\u88fd\uff0c\u7aef\u7c92\u9084\u662f\u6108\u4f86\u6108\u77ed\u3002\u6700\u5f8c\uff0c\u7d30\u80de\u4fbf\u4e0d\u518d\u80fd\u5206\u88c2\uff0c\u8d70\u5165\u8001\u5316\u3001\u6b7b\u4ea1\u3002<\/p>\n

\u79d1\u5b78\u5bb6\u5011\u4e00\u76f4\u5728\u8a66\u5716\u627e\u51fa\u53ef\u4ee5\u5ef6\u7de9\u7aef\u7c92\u8b8a\u77ed\u7684\u65b9\u6cd5\u3002\u904e\u53bb\u66fe\u6709\u4eba\u5617\u8a66\u5c07\u8ca0\u8cac\u7522\u751f\u7aef\u7c92\u9176\u86cb\u767d\u8cea\u90e8\u5206(telomerase reverse transcriptase\uff0c\u7c21\u7a31TERT)\u7684mRNA\u653e\u5165\u7d30\u80de\uff0c\u4f7f\u7aef\u7c92\u9176\u7684\u8868\u73fe\u91cf\u5347\u9ad8\uff0c\u4f86\u9020\u6210\u7aef\u7c92\u5ef6\u9577\uff1b\u7d50\u679c\u537b\u7522\u751f\u4e86\u91dd\u5c0d\u7aef\u7c92\u9176\u7684\u514d\u75ab\u53cd\u61c9\u3002\u9019\u6b21\uff0c\u53f2\u4e39\u4f5b\u7684\u7814\u7a76\u5718\u968a\u4f7f\u7528\u4e86\u4fee\u98fe\u904e\u7684TERT\u7684mRNA\uff0c\u4f7f\u7d30\u80de\u4e2d\u7684\u7aef\u7c92\u9176\u7684\u91cf\u53ea\u63d0\u9ad8\u4e8624\u523048\u5c0f\u6642\uff0c\u907f\u514d\u4e86\u514d\u75ab\u53cd\u61c9\u7684\u767c\u751f\uff0c\u4f7f\u5f97\u7d30\u80de\u7684\u7aef\u7c92\u88ab\u5ef6\u9577\u4e86900\u500b\u9e7c\u57fa\u5c0d(base pairs\uff0c\u70ba\u8a08\u7b97DNA\u5927\u5c0f\u7684\u55ae\u4f4d)\u3002<\/p>\n

\u7aef\u7c92\u5ef6\u9577900\u500b\u9e7c\u57fa\u5c0d\u6709\u4ec0\u9ebc\u6548\u679c\u5462\uff1f\u7814\u7a76\u5718\u968a\u767c\u73fe\uff0c\u7576\u7e96\u7dad\u6bcd\u7d30\u80de(fibroblast\uff0c\u53ef\u767c\u80b2\u70ba\u76ae\u819a\u7d30\u80de)\u7684\u7aef\u7c92\u88ab\u5ef6\u9577\u5f8c\uff0c\u5b83\u53ef\u4ee5\u591a\u5206\u88c228\u6b21\uff0c\u9019\u6a23\u53ef\u4ee5\u7522\u751f\u4e0a\u5343\u5104\u500b\u7d30\u80de\uff1b\u800c\u808c\u6bcd\u7d30\u80de(myoblast\uff0c\u53ef\u767c\u80b2\u70ba\u808c\u8089\u7d30\u80de)\u53ef\u4ee5\u591a\u5206\u88c23\u81f34\u6b21\u3002\u7531\u65bc\u9019\u500b\u65b9\u6cd5\u53ea\u662f\u8b93\u7aef\u7c92\u9176\u7684\u6d3b\u6027\u77ed\u66ab\u5730\u63d0\u9ad8\uff0c\u904e\u5f8c\u7d30\u80de\u53c8\u7e7c\u7e8c\u6b63\u5e38\u7684\u8001\u5316\uff0c\u662f\u4e0d\u662f\u5f88\u985e\u4f3c\u65bc\u5c07\u6642\u9418\u56de\u64a5\u4e00\u6a23\u5462\uff1f<\/p>\n

\u53c3\u8003\u6587\u737b\uff1a
\n<\/strong>2015\/1\/23.\u00a0Telomere extension turns back aging clock in cultured human cells, study finds<\/a>. Science Daily.
\nJohn Ramunas, Eduard Yakubov, Jennifer J. Brady, St\u00e9phane Y. Corbel, Colin Holbrook, Moritz Brandt, Jonathan Stein, Juan G. Santiago, John P. Cooke, and Helen M. Blau. 2015.\u00a0Transient delivery of modified mRNA encoding TERT rapidly extends telomeres in human cells<\/a>. FASEB J. doi: 10.1096\/fj.14-259531<\/p>\n

--<\/strong><\/span>
\n \u4f5c\u8005\uff1a\u8449\u7da0\u8212\u3000\u6148\u6fdf\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u52a9\u7406\u6559\u6388\uff0c\u79d1\u6559\u4e2d\u5fc3\u7279\u7d04\u5beb\u624b\uff0c\u5f9e\u4e8b\u79d1\u666e\u6587\u7ae0\u5beb\u4f5c\u3002<\/strong><\/span><\/p>\n

 <\/p>\n

\"\u52a0\u5165\u597d\u53cb\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u64b0\u6587\uff5c\u8449\u7da0\u8212 \u8fd4\u8001\u9084\u7ae5\uff0c\u6216\u662f\u9577\u751f\u4e0d\u8001\uff0c\u662f<\/p>\n","protected":false},"author":20,"featured_media":20764,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3293,1025],"tags":[37,1674,29,1673,727,1543],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2015\/02\/150123102539-large.jpg","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/20716"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=20716"}],"version-history":[{"count":13,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/20716\/revisions"}],"predecessor-version":[{"id":32155,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/20716\/revisions\/32155"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/20764"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=20716"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=20716"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=20716"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}