{"id":20231,"date":"2015-01-12T00:46:44","date_gmt":"2015-01-11T16:46:44","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=20231"},"modified":"2018-08-01T14:38:49","modified_gmt":"2018-08-01T06:38:49","slug":"%e3%80%90%e6%a4%8d%e7%89%a9%e5%a5%a7%e5%a6%99%e3%80%91%e8%be%b2%e6%a1%bf%e8%8f%8c%ef%bc%88agrobacterium-tumefaciens%ef%bc%89%e7%9a%84%e4%b8%8d%e7%a2%ba%e5%ae%9a%e6%80%a7","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=20231","title":{"rendered":"\u3010\u690d\u7269\u5967\u5999\u3011\u8fb2\u687f\u83cc\uff08Agrobacterium tumefaciens\uff09\u7684\u4e0d\u78ba\u5b9a\u6027"},"content":{"rendered":"

\u25a0\u73fe\u5728\u4f5c\u690d\u7269\u7684\u8f49\u6b96\uff0c\u5e7e\u4e4e\u90fd\u662f\u7528\u8fb2\u687f\u83cc\u4e86\u3002\u683c\u862d\u6c0f\u9670\u6027\uff08Gram negative\uff09\u687f\u83cc\uff0c\u539f\u672c\u662f\u8fb2\u4f5c\u7269\u7684\u75c5\u83cc\uff0c\u6703\u5c0e\u81f4\u690d\u7269\u7522\u751f\u816b\u7624\uff08crown gall\uff09\uff1b\u96d6\u7136\u690d\u7269\u7684\u816b\u7624\u4e0d\u6703\u8f49\u79fb\u4e5f\u6c92\u6709\u81f4\u547d\u7684\u98a8\u96aa\uff0c\u537b\u6703\u4f7f\u690d\u7269\u751f\u7522\u7684\u990a\u5206\uff0c\u56e0\u70ba\u8981\u4f9b\u61c9\u7d66\u816b\u7624\u800c\u9020\u6210\u7522\u91cf\u6e1b\u5c11\u3002<\/strong><\/span><\/p>\n

\"Agrobacteriumgall\"
\u8fb2\u687f\u83cc\u6240\u7522\u751f\u7684\u816b\u7624\u3002\u5716\u7247\u4f86\u6e90\uff1awiki<\/figcaption><\/figure>\n

\u64b0\u6587\uff5c<\/strong>\u8449\u7da0\u8212<\/p>\n

\u8fb2\u687f\u83cc\u6240\u9020\u6210\u7684\u5371\u5bb3\uff0c\u6700\u65e9\u662f\u5728\u8461\u8404\u88e1\u9762\u767c\u73fe\uff1b\u5f8c\u4f86\u6162\u6162\u767c\u73fe\u5b83\u5176\u5be6\u53ef\u4ee5\u5371\u5bb3\u8a31\u591a\u690d\u7269\uff0c\u4e0d\u904e\u5728\u81ea\u7136\u754c\u4e2d\u4ee5\u96d9\u5b50\u8449\u690d\u7269\u5c45\u591a\u3002\u8fb2\u687f\u83cc\u81ea\u5df1\u5e36\u4e86\u4e00\u500b\u8cea\u9ad4\uff0c\u7a31\u70baTi\u8cea\u9ad4\u3002\u9019\u500bTi\u8cea\u9ad4\uff0c\u5728\u8fb2\u687f\u83cc\u611f\u67d3\u690d\u7269\u6642\uff0c\u5176\u4e2d\u6709\u4e00\u6bb5DNA\u6703\u63d2\u5165\u690d\u7269\u7684\u57fa\u56e0\u9ad4\u4e2d\u3002<\/p>\n

\u9019\u6bb5DNA\uff08\u7a31\u70baT-DNA\uff09\u88e1\u9762\u5e36\u6709\u5408\u6210\u5169\u7a2e\u690d\u7269\u8cc0\u723e\u8499\u6240\u9700\u7684\u9175\u7d20\u57fa\u56e0\uff0c\u9032\u5165\u690d\u7269\u57fa\u56e0\u9ad4\u5f8c\uff0c\u4fbf\u6703\u4f7f\u5f97\u8a72\u690d\u7269\u7d30\u80de\u958b\u59cb\u5927\u91cf\u7522\u751f\u751f\u9577\u7d20\uff08auxin\uff09\u8207\u7d30\u80de\u5206\u88c2\u7d20\uff08cytokinin\uff09\u3002\u9019\u5169\u7a2e\u8cc0\u723e\u8499\u76f8\u52a0\u7684\u7d50\u679c\uff0c\u6703\u4f7f\u5f97\u7d30\u80de\u958b\u59cb\u5206\u88c2\u589e\u751f\uff0c\u65bc\u662f\u5c31\u7522\u751f\u816b\u7624\u4e86\u3002\u7576\u7136\uff0cT-DNA\u4e2d\u9084\u6709\u5176\u4ed6\u7684\u57fa\u56e0\uff0c\u6703\u9a45\u4f7f\u690d\u7269\u7d30\u80de\u5408\u6210\u8fb2\u687f\u83cc\u6240\u9700\u8981\u7684\u990a\u5206\uff0c\u5305\u62ec\u4e86\u5c0f\u5206\u5b50\u6c28\u57fa\u9178\u8207\u78f7\u9178\u5316\u7684\u7cd6\u985e\u7b49\uff0c\u9019\u6a23\u8fb2\u687f\u83cc\u5728\u816b\u7624\u4e2d\u624d\u80fd\u751f\u9577\u58ef\u5927\u3002<\/p>\n

\u539f\u672c\u7814\u7a76\u8fb2\u687f\u83cc\u662f\u70ba\u4e86\u8981\u6253\u6557\u5b83\uff0c\u4f46\u662f\u79d1\u5b78\u5bb6\u5728\u7814\u7a76\u7684\u904e\u7a0b\u4e2d\u767c\u73fe\uff0c\u9019\u96bb\u7d30\u83cc\u975e\u5e38\u7684\u6709\u610f\u601d\u3002\u539f\u4f86\uff0c\u8fb2\u687f\u83cc\u5728\u5c07T-DNA\u8f49\u5165\u690d\u7269\u57fa\u56e0\u9ad4\u6642\uff0c\u6839\u672c\u4e0d\u7ba1\u88e1\u9762\u6709\u4ec0\u9ebc\u3001\u4e5f\u4e0d\u7ba1\u6709\u591a\u9577\uff08\u7576\u7136\uff0c\u592a\u9577\u4e86\u6548\u7387\u6703\u964d\u4f4e\uff09\uff0c\u5b83\u53ea\u8a8d\u9019\u6bb5DNA\u7684\u5169\u908a\u908a\u754c\uff08\u7a31\u70ba\u5de6\u53f3\u908a\u754c\uff09\uff0c\u7136\u5f8c\u5c31\u4e00\u80a1\u8166\u5730\u5c07\u5de6\u53f3\u908a\u754c\u4e4b\u9593\u7684DNA\u7d66\u585e\u9032\u690d\u7269\u88e1\u9762\u4e86\u3002<\/p>\n

\"\u8fb2\u687f\u83cc\u7684\u8f49\u6b96\u539f\u7406\u3002\u5de6\u908a\u662f\u8fb2\u687f\u83cc\uff0c\u53f3\u908a\u662f\u690d\u7269\u7d30\u80de\uff1b
\u8fb2\u687f\u83cc\u7684\u8f49\u6b96\u539f\u7406\u3002\u5de6\u908a\u662f\u8fb2\u687f\u83cc\uff0c\u53f3\u908a\u662f\u690d\u7269\u7d30\u80de\uff1b
C\u70baTi\u8cea\u9ad4\uff0c\u8f09\u9ad4\u4e2d\u7684a\u70baT-DNA\u3002\u5716\u7247\u4f86\u6e90\uff1awiki<\/figcaption><\/figure>\n

\u9019\u6a23\u6709\u610f\u601d\u7684\u8f49\u6b96\u904e\u7a0b\uff0c\u5c31\u8b93\u79d1\u5b78\u5bb6\u5011\u60f3\u5230\uff0c\u5982\u679c\u53ef\u4ee5\u628aT-DNA\u7528\u81ea\u5df1\u60f3\u8981\u653e\u9032\u690d\u7269\u88e1\u9762\u7684\u57fa\u56e0\u7d66\u53d6\u4ee3\uff0c\u662f\u4e0d\u662f\u5c31\u53ef\u4ee5\u628a\u300c\u4efb\u4f55\u57fa\u56e0\u300d\u90fd\u7d66\u5b83\u653e\u9032\u53bb\u4e86\u5462\uff1f<\/p>\n

\u7406\u8ad6\u4e0a\u662f\u9019\u6a23\uff0c\u4e0d\u904e\u56e0\u70baTi\u8cea\u9ad4\u5be6\u5728\u662f\u592a\u5927\u4e86\uff08\u5927\u7d04200kb\u4ee5\u4e0a\uff09\uff0c\u9020\u6210\u5728\u5be6\u9a57\u5ba4\u88e1\u9762\u7684\u64cd\u4f5c\u5f88\u56f0\u96e3\u3002\u9019\u500b\u969c\u7919\uff0c\u76f4\u52301983\u5e74 Hoekema \u4ee5\u53cade Frammond\u9019\u5169\u500b\u7814\u7a76\u5718\u968a\u767c\u73fe\uff0c\u53ef\u4ee5\u628aTi\u8cea\u9ad4\u4e00\u5206\u70ba\u4e8c\u4e4b\u5f8c\uff0c\u8fb2\u687f\u83cc\u624d\u6210\u70ba\u7814\u7a76\u690d\u7269\u7684\u4eba\u7684\u5fc3\u982d\u5bf6\u3002<\/p>\n

\u5f9e\u6b64\u4ee5\u5f8c\uff0c\u8fb2\u687f\u83cc\u958b\u59cb\u88ab\u5927\u91cf\u7528\u5728\u88fd\u4f5c\u8f49\u57fa\u56e0\u690d\u7269\u4e0a\uff1b\u539f\u672c\u662f\u96d9\u5b50\u8449\u690d\u7269\u7684\u75c5\u539f\u83cc\u7684\u5b83\uff0c\u7d93\u904e\u7814\u7a76\u5718\u968a\u6089\u5fc3\u8abf\u88fd\u914d\u65b9\u4ee5\u5f8c\uff0c\u5b83\u73fe\u5728\u5df2\u7d93\u53ef\u4ee5\u8aaa\u662f\u5c11\u6709\u6575\u624b\u3002\u8ddf\u57fa\u56e0\u69cd\u76f8\u6bd4\uff0c\u53c8\u4e0d\u7528\u8cb7\u5f88\u8cb4\u7684\u5100\u5668\u3001\u4e5f\u4e0d\u9700\u8981\u8cb7\u91d1\u7c89\u6216\u93a2\u7c89\uff1b\u53ea\u8981\u628a\u8cea\u9ad4\u505a\u597d\uff0c\u585e\u5230\u8fb2\u687f\u83cc\u88e1\u9762\uff0c\u990a\u500b\u4e00\u5806\u4ee5\u5f8c\uff0c\u628a\u8981\u8f49\u7684\u7d44\u7e54\u6ce1\u5728\u88e1\u9762\u82e5\u5e72\u6642\u9593\uff0c\u5c31\u5927\u529f\u544a\u6210\u4e86\uff01\u5b83\u80fd\u5920\u6210\u70ba\u690d\u7269\u7814\u7a76\u8005\u7684\u5fc3\u982d\u5bf6\uff0c\u771f\u7684\u4e0d\u662f\u84cb\u7684\uff01<\/p>\n

\u4f46\u662f\uff0c\u5b83\u9084\u662f\u6709\u4e00\u4e9b\u9650\u5236\u3002\u904e\u53bb\u7684\u7814\u7a76\u767c\u73fe\uff0c\u7531\u65bc\u8fb2\u687f\u83cc\u53ea\u9700\u8981\u5728\u690d\u7269\u57fa\u56e0\u9ad4\u4e0a\u627e\u5230\u5f88\u5c0f\u4e00\u6bb5\u5e8f\u5217\u76f8\u4f3c\uff0c\u5c31\u53ef\u4ee5\u9032\u884c\u8f49\u6b96\uff1b\u65bc\u662f\u5e7e\u4e4e\u53ef\u4ee5\u8aaa\uff0c\u8fb2\u687f\u83cc\u5728\u690d\u7269\u57fa\u56e0\u9ad4\u4e2d\u7684\u300c\u4efb\u4f55\u5730\u65b9\u300d\u90fd\u53ef\u4ee5\u628aT-DNA\u585e\u9032\u53bb\u3002\u9019\u6a23\u96d6\u7136\u5f88\u65b9\u4fbf\uff0c\u4f46\u4e5f\u6709\u9ebb\u7169\u3002\u6709\u6642\u5b83\u6703\u7834\u58de\u57fa\u56e0\uff0c\u9020\u6210\u690d\u7269\u7522\u751f\u4e00\u4e9b\u7279\u6b8a\u7684\u6027\u72c0\u3002<\/p>\n

\u7576\u7136\uff0c\u90a3\u6a23\u7684\u57fa\u6539\u690d\u7269\uff0c\u5c31\u4e0d\u80fd\u4f7f\u7528\u4e86\uff1b\u96d6\u7136\u5b83\u9084\u53ef\u4ee5\u6709\u5176\u4ed6\u7684\u7528\u9014\u3002\u4e0d\u904e\uff0c\u6709\u53e6\u5916\u4e00\u500b\u7591\u554f\u662f\uff1a\u8fb2\u687f\u83cc\u771f\u7684\u6709\u90a3\u9ebc\u300c\u4e56\u300d\uff0c\u5c31\u53ea\u6703\u628a\u5de6\u53f3\u908a\u754c\u4e4b\u9593\u7684DNA\u585e\u9032\u690d\u7269\u88e1\u9762\u800c\u5df2\u55ce\uff1f<\/p>\n

\u9019\u500b\u7591\u554f\uff0c\u5982\u679c\u554f\u65e9\u671f\u7814\u7a76\u690d\u7269\u7684\u79d1\u5b78\u5bb6\uff0c\u53ef\u80fd\u6703\u88ab\u55e4\u4e4b\u4ee5\u9f3b\uff1b\u4ed6\u5011\u6703\u544a\u8a34\u4f60\uff0c\u7576\u7136\uff01\u4e0d\u904e\u9019\u500b\u7576\u7136\uff0c\u57281990\u5e74\u4ee3\u5df2\u7d93\u88ab\u6253\u7834\u4e86\u3002<\/p>\n

\u57281992\u5e7412\u67082\u65e5\uff0c\u7576\u52a0\u57fa\u516c\u53f8\uff08Calgene Inc\uff09\u6b63\u5728\u70ba\u4e86\u5b83\u5011\u7684\u838e\u5f17\u756a\u8304\uff08Flavr Savr Tomato\uff09\u7533\u8acb\u4e0a\u5e02\u5fd9\u5f97\u5929\u7ffb\u5730\u8986\u6642\uff0c\u6536\u5230\u4e86\u98df\u54c1\u85e5\u7269\u7ba1\u7406\u5c40\u7684\u4f86\u51fd\uff0c\u5e0c\u671b\u4ed6\u5011\u6f84\u6e05\u4e00\u4ef6\u4e8b\uff1a\u662f\u5426\u771f\u7684\u53ea\u6709T-DNA\u7684\u5e8f\u5217\u5d4c\u5165\u756a\u8304\u4e2d\uff1f<\/p>\n

\u539f\u672c\u52a0\u57fa\u516c\u53f8\u4e5f\u8a8d\u70ba\u98df\u54c1\u85e5\u7269\u7ba1\u7406\u5c40\u672a\u514d\u591a\u6b64\u4e00\u554f\uff0c\u4f46\u7b49\u5230\u5357\u65b9\u58a8\u9ede\u8a66\u9a57\uff08Southern Blot\uff09\u51fa\u7210\uff0c\u4ed6\u5011\u5f88\u9a5a\u8a1d\u5730\u767c\u73fe\uff1a\u7d04\u670920%-30%\u7684\u8fb2\u687f\u83cc\u8f09\u9ad4\u5e8f\u5217\u4e5f\u51fa\u73fe\u5728\u690d\u7269\u4e2d\uff08\u53c31\uff09\u3002\u65bc\u662f\u4ed6\u5011\u53ea\u5f97\u5c07\u9019\u4e9b\u542b\u6709\u8fb2\u687f\u83cc\u8f09\u9ad4\u5e8f\u5217\u7684\u756a\u8304\u5254\u9664\u3002<\/p>\n

\u5f8c\u4f86\uff0cNeal Gutterson\u7b49\u4eba\u4e5f\u767c\u73fe\uff0c\u8fb2\u687f\u83cc\u7684\u8f09\u9ad4\u5e8f\u5217\u7684\u78ba\u6703\u51fa\u73fe\u5728\u57fa\u6539\u690d\u7269\u4e2d\uff1b\u800c\u4e14\u6bd4\u4f8b\u9ad8\u65bc\u52a0\u57fa\u516c\u53f8\u6240\u505a\u51fa\u4f86\u7684\u7d50\u679c\uff0850%~80%\uff09\u3002\u4e0d\u904e\uff0cGutterson\u7684\u7814\u7a76\u5718\u968a\uff0c\u5f8c\u4f86\u4fbf\u5728\u8f09\u9ad4\u4e2d\u653e\u4e86\u4e00\u500b\u81f4\u6b7b\u57fa\u56e0\uff0c\u53ea\u8981\u8f49\u6b96\u904e\u7a0b\u4e2d\u628a\u5305\u542b\u4e86\u81f4\u6b7b\u57fa\u56e0\u7684\u8f09\u9ad4\u5e8f\u5217\u653e\u9032\u53bb\u4e86\uff0c\u690d\u7269\u4fbf\u6703\u6b7b\u4ea1\uff08\u53c32\uff09\u3002<\/p>\n

\u770b\u8d77\u4f86\u4f3c\u4e4e\u984d\u5916\u7684\u5e8f\u5217\u52a0\u5165\u690d\u7269\u7684\u554f\u984c\u89e3\u6c7a\u4e86\uff0c\u4f46\u7b46\u8005\u904e\u53bb\u7684\u7d93\u9a57\u767c\u73fe\uff0c\u8fb2\u687f\u83cc\u6709\u6642\u6703\u53ea\u5d4c\u5165\u77ed\u77ed\u7684\u5e8f\u5217\uff08\u53ea\u6709\u5e7e\u500b\u6216\u5341\u5e7e\u500b\u9e7c\u57fa\u5c0d\uff09\uff0c\u9019\u77ed\u77ed\u7684\u5e8f\u5217\uff0c\u5982\u679c\u63d2\u5165\u57fa\u56e0\u4e2d\uff0c\u4ecd\u8db3\u4ee5\u7834\u58de\u86cb\u767d\u8cea\u7684\u7522\u751f\uff0c\u6216\u662f\u6253\u4e82\u57fa\u56e0\u7684\u8868\u73fe\uff1b\u53ef\u662f\u9019\u6a23\u77ed\u7684\u5e8f\u5217\uff0c\u537b\u7121\u6cd5\u4ee5\u5357\u65b9\u58a8\u9ede\u8a66\u9a57\u9451\u5b9a\uff0c\u5d4c\u5165\u81f4\u6b7b\u57fa\u56e0\u4e5f\u7121\u6cd5\u9810\u9632\u9019\u6a23\u7684\u4e8b\u60c5\u767c\u751f...\u6240\u4ee5\uff0c\u7a76\u7adf\u8fb2\u687f\u83cc\u7528\u4f86\u8f49\u6b96\uff0c\u662f\u5426\u771f\u7684\u662f\u5b89\u5168\u7684\uff1f\u9664\u537b\u5b9a\u5e8f\u6574\u500b\u57fa\u56e0\u9ad4\uff08\u9700\u8981\u76f8\u7576\u9ad8\u7684\u7d93\u8cbb\uff09\u4e4b\u5916\uff0c\u901a\u5e38\u90fd\u662f\u7531\u6aa2\u9a57\u5916\u89c0\u6027\u72c0\u4ee5\u53ca\u57fa\u6539\u690d\u7269\u5404\u5927\u5c0f\u5206\u5b50\u7684\u8868\u73fe\u91cf\u662f\u5426\u7570\u65bc\u539f\u690d\u7269\u800c\u5df2\u3002<\/p>\n

\u6240\u4ee5\uff0c\u662f\u5426\u5b89\u5168\u5462\uff1f\u7b46\u8005\u4e5f\u6c92\u6709\u7b54\u6848\uff0c\u60a8\u5462\uff1f<\/p>\n

\u53c3\u8003\u6587\u737b\uff1a<\/strong>
\n1. Belinda Martineau.\u00a0\u756a\u8304\u4e00\u865f-\u5168\u7403\u7b2c\u4e00\u500b\u4e0a\u5e02\u57fa\u6539\u98df\u54c1\u300c\u838e\u5f17\u756a\u8304\u300d\u7684\u8d77\u8207\u843d<\/a>\u3002\u9060\u6d41\u51fa\u7248\u3002
\n2. Bill Hanson, Dean Engler, York Moy, Bob Newman, Ed Ralston and Neal Gutterson. 1999.\u00a0A simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences<\/a>. The Plant Journal. 19\uff086\uff09\uff1a727\u2013734.
\n--<\/strong><\/span>
\n \u4f5c\u8005\uff1a\u8449\u7da0\u8212\u3000\u6148\u6fdf\u5927\u5b78\u751f\u547d\u79d1\u5b78\u7cfb\u52a9\u7406\u6559\u6388\uff0c\u79d1\u6559\u4e2d\u5fc3\u7279\u7d04\u5beb\u624b\uff0c\u5f9e\u4e8b\u79d1\u666e\u6587\u7ae0\u5beb\u4f5c\u3002<\/strong><\/span><\/p>\n

 <\/p>\n

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