{"id":19420,"date":"2014-10-09T10:02:20","date_gmt":"2014-10-09T02:02:20","guid":{"rendered":"https:\/\/case.ntu.edu.tw\/blog\/?p=19420"},"modified":"2017-03-31T23:16:50","modified_gmt":"2017-03-31T15:16:50","slug":"%e3%80%90%e6%99%82%e4%ba%8b%e7%84%a6%e9%bb%9e%e3%80%912014%e5%b9%b4%e8%ab%be%e8%b2%9d%e7%88%be%e5%8c%96%e5%ad%b8%e7%8d%8e%ef%bc%8d%e5%a6%82%e4%bd%95%e5%b0%87%e5%85%89%e5%ad%b8%e9%a1%af%e5%be%ae","status":"publish","type":"post","link":"https:\/\/case.ntu.edu.tw\/blog\/?p=19420","title":{"rendered":"\u3010\u6642\u4e8b\u7126\u9ede\u30112014\u5e74\u8afe\u8c9d\u723e\u5316\u5b78\u734e\uff0d\u5982\u4f55\u5c07\u5149\u5b78\u986f\u5fae\u93e1\u8b8a\u6210\u5948\u7c73\u986f\u5fae\u93e1"},"content":{"rendered":"<p><span style=\"color: #333399;\"><strong>\u25a0\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c(Eric Betzig)\uff0c\u53f2\u8482\u82ac\uff65\u6d77\u723e(Stefan W. Hell)\u4ee5\u53ca\u5a01\u5ec9\uff65\u83ab\u7d0d(William E. Moerner)\u7b49\u4e09\u4eba\u5f97\u5230\u4e862014\u5e74\u7684\u8afe\u8c9d\u723e\u5316\u5b78\u734e\uff0c\u9019\u662f\u56e0\u70ba\u4ed6\u5011\u8d8a\u904e\u4e86\u4e00\u500b\u79d1\u5b78\u4e0a\u8a2d\u60f3\u7684\u9650\u5236\uff0c\u4e5f\u5c31\u662f\u4e00\u500b\u5149\u5b78\u986f\u5fae\u93e1\u6c38\u9060\u7121\u6cd5\u8d85\u8d8a0.2\u5fae\u7c73\u7684\u89e3\u6790\u5ea6\u898f\u683c\u3002\u5229\u7528\u5206\u5b50\u7684\u87a2\u5149\uff0c\u79d1\u5b78\u5bb6\u73fe\u5728\u53ef\u4ee5\u76e3\u770b\u5728\u7d30\u80de\u5167\u90e8\u5206\u5b50\u4e4b\u9593\u7684\u76f8\u4e92\u4f5c\u7528\uff1b\u4ed6\u5011\u53ef\u4ee5\u89c0\u5bdf\u8207\u75be\u75c5\u76f8\u95dc\u7684\u86cb\u767d\u8cea\u4e4b\u805a\u96c6\uff0c\u4e5f\u53ef\u4ee5\u5728\u5948\u7c73\u7684\u5c3a\u5ea6\u88e1\u8ffd\u8e64\u7d30\u80de\u7684\u5206\u88c2\u3002<\/strong><\/span><\/p>\n<figure id=\"attachment_19430\" aria-describedby=\"caption-attachment-19430\" style=\"width: 400px\" class=\"wp-caption alignleft\"><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-19430\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/857030-300x170.jpg\" alt=\"Photos: M. Staley\/HHMI, B. Schuller, Max-Planck-Institut, Wikimedia Commons, CC-BY-SA-3.0\" width=\"400\" height=\"227\" srcset=\"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/857030-300x170.jpg 300w, https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/857030.jpg 635w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><figcaption id=\"caption-attachment-19430\" class=\"wp-caption-text\">Photos: M. Staley\/HHMI, B. Schuller, Max-Planck-Institut, Wikimedia Commons, CC-BY-SA-3.0<\/figcaption><\/figure>\n<p><strong>\u7de8\u8b6f\uff5c<\/strong>\u53f0\u5927\u5316\u5b78\u7cfb\u6559\u6388\u3000\u8521\u860a\u660e<\/p>\n<p>\u7d05\u8840\u7403\u7d30\u80de\u3001\u7d30\u83cc\u3001\u9175\u6bcd\u83cc\u7d30\u80de\u4ee5\u53ca\u6e38\u52d5\u7cbe\u5b50\uff1a\u7576\u79d1\u5b78\u5bb6\u5728\u5341\u4e03\u4e16\u7d00\u7b2c\u4e00\u6b21\u958b\u59cb\u5728\u986f\u5fae\u93e1\u4e0b\u7814\u7a76\u6d3b\u9ad4\u7d44\u7e54\u6642\uff0c\u4e00\u500b\u65b0\u7684\u4e16\u754c\u5728\u4ed6\u5011\u7684\u773c\u524d\u6253\u958b\u3002\u9019\u662f\u5fae\u751f\u7269\u5b78\u51fa\u4e16\u4e4b\u969b\uff0c\u5f9e\u6b64\u4e4b\u5f8c\uff0c\u5149\u5b78\u986f\u5fae\u93e1\u6210\u70ba\u751f\u547d\u79d1\u5b78\u5bb6\u5de5\u5177\u7bb1\u88e1\u9762\u6700\u91cd\u8981\u7684\u5de5\u5177\u4e4b\u4e00\u3002\u5176\u5b83\u7684\u986f\u5fae\u93e1\u8853\uff0c\u4f8b\u5982\u96fb\u5b50\u986f\u5fae\u93e1\uff0c\u5176\u6240\u9700\u7684\u6e96\u5099\u65b9\u6cd5\u6700\u7d42\u6703\u6bba\u6b7b\u7d30\u80de\u3002<\/p>\n<p><strong>\u767c\u4eae\u7684\u5206\u5b50\u8d8a\u904e\u4e86\u7269\u7406\u7684\u5c4f\u969c<br \/>\n<\/strong>\u7136\u800c\uff0c\u6709\u4e00\u6bb5\u5f88\u9577\u7684\u6642\u9593\uff0c\u5149\u5b78\u986f\u5fae\u93e1\u88ab\u4e00\u500b\u7269\u7406\u7684\u5c4f\u969c\u6240\u963b\u7919\uff0c\u9650\u5236\u4e86\u6240\u80fd\u89e3\u6790\u7684\u7d50\u69cb\u5927\u5c0f\u3002\u57281873\u5e74\uff0c\u986f\u5fae\u93e1\u5b78\u5bb6\u6069\u65af\u7279\uff65\u963f\u8c9d(Ernst Abbe)\u767c\u8868\u4e86\u4e00\u500b\u65b9\u7a0b\u5f0f\uff0c\u8b49\u660e\u4e86\u5149\u5b78\u986f\u5fae\u93e1\u7684\u89e3\u6790\u5ea6\u662f\u5982\u4f55\u53d7\u5230\u5149\u7684\u6ce2\u9577\uff0c\u4ee5\u53ca\u4e00\u4e9b\u5176\u5b83\u7684\u56e0\u7d20\u6240\u9650\u5236\u3002\u56e0\u6b64\u9019\u5c0e\u81f4\u79d1\u5b78\u5bb6\u5011\uff0c\u5728\u4e8c\u5341\u4e16\u7d00\u7684\u5927\u534a\u6642\u9593\u88e1\uff0c\u76f8\u4fe1\u5149\u5b78\u986f\u5fae\u93e1\u662f\u6c38\u9060\u7121\u6cd5\u7528\u4f86\u89c0\u5bdf\u90a3\u4e9b\u6bd4\u6240\u7528\u7684\u5149\u4e4b\u6ce2\u9577\u7684\u4e00\u534a\u9084\u5c0f\u7684\u7269\u9ad4\uff0c\u4e5f\u5c31\u662f0.2\u5fae\u7c73(200\u5948\u7c73\uff1b\u5fae\u7c73 =  <span class='MathJax_Preview'><img src='https:\/\/case.ntu.edu.tw\/blog\/wp-content\/plugins\/latex\/cache\/tex_dbed30b87205a65397b356aa3d6a40e3.gif' style='vertical-align: middle; border: none; padding-bottom:2px;' class='tex' alt=\"\" \/><\/span><script type='math\/tex'><\/script> \u7c73=  <span class='MathJax_Preview'><img src='https:\/\/case.ntu.edu.tw\/blog\/wp-content\/plugins\/latex\/cache\/tex_1f957d32ccdbb6bc5dbcb3f90cbe65a5.gif' style='vertical-align: middle; border: none; padding-bottom:2px;' class='tex' alt=\"\" \/><\/span><script type='math\/tex'><\/script> \u5948\u7c73) (\u5716\u4e00)\u3002\u7d30\u80de\u88e1\u4e00\u4e9b\u80de\u5668\u7684\u8f2a\u5ed3\uff0c\u4f8b\u5982\u7d30\u80de\u7684\u767c\u96fb\u6a5f\u7c92\u7dda\u9ad4\uff0c\u96d6\u53ef\u4ee5\u770b\u5230\uff0c\u4f46\u662f\u5e7e\u4e4e\u4e0d\u53ef\u80fd\u5206\u8fa8\u66f4\u5c0f\u7684\u7269\u9ad4\uff0c\u56e0\u6b64\u8b6c\u5982\u60f3\u8981\u8ffd\u8e64\u7d30\u80de\u88e1\u86cb\u767d\u8cea\u5206\u5b50\u4e4b\u9593\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5c31\u7121\u6cd5\u505a\u5230\uff0c\u9019\u597d\u6bd4\u80fd\u770b\u5230\u4e00\u500b\u57ce\u5e02\u7684\u5efa\u7bc9\u7269\uff0c\u4f46\u537b\u7121\u6cd5\u770b\u51fa\u5e02\u6c11\u5982\u4f55\u7684\u751f\u6d3b\uff0c\u548c\u5982\u4f55\u70ba\u5176\u751f\u5b58\u800c\u52aa\u529b\u3002\u70ba\u4e86\u77ad\u89e3\u4e00\u500b\u7d30\u80de\u5982\u4f55\u7684\u904b\u4f5c\uff0c\u4f60\u5fc5\u9808\u80fd\u8ffd\u8e64\u500b\u5225\u7684\u5206\u5b50\u5982\u4f55\u7684\u5de5\u4f5c\u3002<br \/>\n<!--more--><\/p>\n<figure id=\"attachment_19422\" aria-describedby=\"caption-attachment-19422\" style=\"width: 636px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" class=\"wp-image-19422 size-full\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image002.gif\" alt=\"image002\" width=\"636\" height=\"180\" \/><figcaption id=\"caption-attachment-19422\" class=\"wp-caption-text\">\u5716\u4e00 \u5728\u5341\u4e5d\u4e16\u7d00\u672b\u8449\uff0c\u6069\u65af\u7279\uff65\u963f\u8c9d(Ernst Abbe)\u5b9a\u7fa9\u4e86\u5149\u5b78\u986f\u5fae\u93e1\u7684\u89e3\u6790\u5ea6\u7d04\u70ba\u5149\u6ce2\u9577\u7684\u4e00\u534a\uff0c\u5dee\u4e0d\u591a\u662f0.2\u5fae\u7c73(200\u5948\u7c73)\uff0c\u9019\u610f\u5473\u8457\u79d1\u5b78\u5bb6\u80fd\u5920\u5340\u5225\u4e00\u500b\u5b8c\u6574\u7684\u7d30\u80de\u4ee5\u53ca\u4e00\u4e9b\u7d30\u80de\u5167\u7684\u80de\u5668\uff0c\u4e0d\u904e\u4ed6\u5011\u5c07\u6c38\u9060\u7121\u6cd5\u5206\u8fa8\u5c0f\u5230\u5982\u4e00\u500b\u6b63\u5e38\u5927\u5c0f\u7684\u75c5\u6bd2\uff0c\u6216\u662f\u4e00\u500b\u55ae\u4e00\u7684\u86cb\u767d\u8cea\u5206\u5b50\u3002<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>\u5118\u7ba1\u963f\u8c9d\u7684\u65b9\u7a0b\u5f0f\u4f9d\u7136\u6210\u7acb\uff0c\u4f46\u7e5e\u5c04\u6975\u9650\u7684\u969c\u7919\u4ecd\u88ab\u514b\u670d\u4e86\u3002\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c\uff0c\u53f2\u8482\u82ac\uff65\u6d77\u723e\u4ee5\u53ca\u5a01\u5ec9\uff65\u83ab\u7d0d\u7b49\u4e09\u4eba\u4e4b\u6240\u4ee5\u7372\u5f972014\u5e74\u7684\u8afe\u8c9d\u723e\u5316\u5b78\u734e\uff0c\u5c31\u662f\u56e0\u70ba\u4ed6\u5011\u5229\u7528\u87a2\u5149\u5206\u5b50\uff0c\u5c07\u5149\u5b78\u986f\u5fae\u93e1\u5e36\u9032\u4e86\u53e6\u4e00\u500b\u5883\u754c\u3002\u7406\u8ad6\u4e0a\uff0c\u4e0d\u518d\u5b58\u5728\u6709\u592a\u5c0f\u800c\u7121\u6cd5\u89c0\u5bdf\u7684\u7d50\u69cb\u3002\u5c31\u7d50\u679c\u800c\u8a00\uff0c\u5149\u5b78\u986f\u5fae\u93e1\u8b8a\u6210\u4e86\u5948\u7c73\u986f\u5fae\u93e1\u3002<\/p>\n<p>\u5982\u4f55\u898f\u907f\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u7684\u6545\u4e8b\uff0c\u8981\u5206\u6210\u5169\u689d\u8def\u7dda\u4f86\u8aaa\uff1b\u5169\u500b\u57fa\u65bc\u4e0d\u540c\u7684\u539f\u7406\u6240\u5404\u81ea\u7368\u7acb\u767c\u5c55\u51fa\u7684\u65b9\u6cd5\uff0c\u90fd\u7372\u5f97\u6210\u529f\u3002\u8b93\u6211\u5011\u56de\u6eaf\u52301993\u5e74\uff0c\u5728\u82ac\u862d\u897f\u5357\u90e8\u7684\u4e00\u500b\u5b78\u751f\u516c\u5bd3\u88e1\uff0c\u53f2\u8482\u82ac\uff65\u6d77\u723e\u5728\u7ffb\u95b1\u4e00\u672c\u91cf\u5b50\u5149\u5b78\u7684\u6559\u79d1\u66f8\u6642\uff0c\u5f97\u5230\u4e86\u4e00\u500b\u5f88\u68d2\u7684\u9ede\u5b50\u3002<\/p>\n<p><strong>\u5c0d\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u7684\u9752\u6625\u53db\u9006\u9762\u5c0d\u7684\u662f\u61f7\u7591<br \/>\n<\/strong>\u81ea\u5f9e\u6d77\u723e\u57281990\u5e74\u5f9e\u5fb7\u570b\u6d77\u5fb7\u5821\u5927\u5b78\u53d6\u5f97\u535a\u58eb\u5b78\u4f4d\u4e4b\u5f8c\uff0c\u4ed6\u5c31\u4e00\u76f4\u5728\u5c0b\u627e\u65b9\u6cd5\uff0c\u4f86\u898f\u907f\u963f\u8c9d\u5728\u8d85\u904e\u4e00\u500b\u4e16\u7d00\u4ee5\u524d\u6240\u8a02\u4e0b\u7684\u9650\u5236\u3002\u6311\u6230\u4e00\u500b\u5df2\u7d93\u5efa\u7acb\u7684\u7406\u8ad6\uff0c\u9019\u6a23\u7684\u60f3\u6cd5\u96d6\u5f88\u8a98\u4eba\uff0c\u4f46\u662f\u5728\u5fb7\u570b\u7684\u8cc7\u6df1\u79d1\u5b78\u5bb6\u5011\uff0c\u7528\u61f7\u7591\u9762\u5c0d\u4e86\u4ed6\u7684\u71b1\u60c5\uff0c\u5c0e\u81f4\u4e86\u6d77\u723e\u5f80\u5bd2\u51b7\u7684\u5317\u65b9\u5c0b\u627e\u5e87\u8b77\u6240\u3002\u4e00\u4f4d\u5728\u82ac\u862d\u7279\u723e\u5eab(Turku)\u5927\u5b78\u7814\u7a76\u87a2\u5149\u986f\u5fae\u93e1\u8853\u7684\u6559\u6388\uff0c\u7d66\u4e86\u6d77\u723e\u5728\u5176\u7814\u7a76\u5c0f\u7d44\u5de5\u4f5c\u7684\u4e00\u500b\u8077\u4f4d\u3002\u6d77\u723e\u76f8\u4fe1\u4e00\u5b9a\u6709\u4e00\u500b\u6a5f\u6703\u80fd\u5920\u514b\u670d\u963f\u8c9d\u7684\u7e5e\u5c04\u6975\u9650\uff0c\u800c\u7576\u4ed6\u8b80\u5230\u90a3\u672c\u91cf\u5b50\u5149\u5b78\u8ab2\u672c\u88e1\u9762\u201c\u53d7\u6fc0\u653e\u5c04\u201d\u7684\u5b57\u8a9e\u6642\uff0c\u5728\u4ed6\u7684\u8166\u6d77\u88e1\u6d6e\u73fe\u4e86\u4e00\u500b\u65b0\u7684\u60f3\u6cd5\uff1a\u201c\u5728\u90a3\u500b\u77ac\u9593\uff0c\u66d9\u5149\u5728\u6211\u8166\u969b\u51fa\u73fe\uff0c\u6211\u7d42\u65bc\u627e\u5230\u4e00\u500b\u5be6\u969b\u7684\u89c0\u5ff5\u4f86\u8ffd\u6c42 \uff0d\u00a0\u4e00\u689d\u771f\u6b63\u7684\u7dda\u7d22\u3002\u201d\u9019\u662f\u4ed6\u65bc2009\u5e74\u81ea\u5df1\u7684\u8aaa\u660e\uff0d \u8b93\u6211\u5011\u9032\u5165\u4ed6\u7684\u60f3\u6cd5\u4e00\u63a2\u7a76\u7adf\u3002<\/p>\n<p><strong>\u89e3\u7b54\uff1a\u7528\u4e00\u500b\u5948\u7c73\u5927\u5c0f\u7684\u624b\u96fb\u7b52\u6383\u63cf\u6a23\u54c1<br \/>\n<\/strong>\u5728\u7279\u723e\u5eab\u5927\u5b78\uff0c\u6d77\u723e\u5728\u9032\u884c\u7a31\u70ba\u87a2\u5149\u986f\u5fae\u93e1\u8853\u7684\u7814\u7a76\uff0c\u90a3\u662f\u4e00\u7a2e\u5229\u7528\u87a2\u5149\u5206\u5b50\u4f86\u8b93\u7d30\u80de\u986f\u50cf\u7684\u6280\u8853\u3002\u8209\u4f8b\u4f86\u8aaa\uff0c\u4ed6\u5011\u53ef\u4ee5\u4f7f\u7528\u53ea\u5c08\u4e00\u7684\u8207\u7d30\u80de\u7684DNA\u5076\u5408\u4e4b\u87a2\u5149\u6297\u9ad4\uff0c\u79d1\u5b78\u5bb6\u5011\u7528\u4e00\u500b\u77ed\u66ab\u7684\u8108\u885d\u5149\u4f86\u6fc0\u767c\u6297\u9ad4\uff0c\u9019\u6703\u8b93\u6297\u9ad4\u767c\u4eae\u4e26\u6301\u7e8c\u4e00\u500b\u77ed\u66ab\u7684\u6642\u9593\uff0c\u5982\u679c\u6297\u9ad4\u662f\u8207DNA\u5076\u5408\u7684\uff0c\u5b83\u5011\u5c31\u6703\u5728\u7d30\u80de\u7576\u4e2d\u653e\u5149\uff0c\u56e0\u70baDNA\u662f\u585e\u5728\u7d30\u80de\u6838\u88e1\u9762\u7684\u3002\u5229\u7528\u9019\u500b\u65b9\u6cd5\uff0c\u79d1\u5b78\u5bb6\u5011\u53ef\u4ee5\u770b\u5230\u67d0\u4e9b\u5206\u5b50\u7684\u4f4d\u7f6e\uff0c\u4f46\u662f\u4ed6\u5011\u53ea\u80fd\u5b9a\u51fa\u4e00\u7fa4\u805a\u96c6\u5728\u4e00\u8d77\u7684\u5206\u5b50\u4e4b\u4f4d\u7f6e\uff0c\u4f8b\u5982\u4e00\u4e9b\u7cfe\u7e8f\u5728\u4e00\u8d77\u7684\u591a\u80a1DNA\uff0c\u4f46\u662f\u56e0\u70ba\u89e3\u6790\u5ea6\u592a\u4f4e\uff0c\u800c\u7121\u6cd5\u5206\u8fa8\u55ae\u80a1\u7684DNA\uff0c\u9019\u5c31\u597d\u50cf\u4f60\u53ef\u4ee5\u770b\u5230\u4e00\u5377\u7d17\u7dda\uff0c\u4f46\u537b\u7121\u6cd5\u770b\u51fa\u7d17\u7dda\u662f\u5982\u4f55\u7e8f\u7e5e\u7684\u3002<\/p>\n<p>\u7576\u6d77\u723e\u8b80\u5230\u53d7\u6fc0\u653e\u5c04\u6642\uff0c\u4ed6\u9ad4\u8a8d\u5230\u61c9\u8a72\u53ef\u4ee5\u8a2d\u8a08\u4e00\u7a2e\u5948\u7c73\u7684\u624b\u96fb\u7b52\uff0c\u80fd\u5920\u5c0d\u8457\u6a23\u54c1\u4ee5\u4e00\u6b21\u4e00\u500b\u5948\u7c73\u7684\u65b9\u5f0f\u6383\u63cf\u3002\u5229\u7528\u53d7\u6fc0\u653e\u5c04\uff0c\u79d1\u5b78\u5bb6\u5011\u53ef\u4ee5\u5c07\u5206\u5b50\u7684\u87a2\u5149\u6dec\u6ec5\uff0c\u7576\u4ed6\u5011\u5c07\u4e00\u9053\u96f7\u5c04\u5149\u675f\u7167\u5728\u90a3\u4e9b\u767c\u5149\u7684\u7684\u5206\u5b50\u4e0a\u6642\uff0c\u5b83\u5011\u6703\u7acb\u523b\u7684\u5931\u53bb\u80fd\u91cf\u800c\u8b8a\u6697\u3002\u57281994\u5e74\uff0c\u6d77\u723e\u767c\u8868\u4e86\u4e00\u7bc7\u8ad6\u6587\u6982\u7565\u8aaa\u660e\u4e86\u4ed6\u7684\u60f3\u6cd5\uff0c\u4ed6\u898f\u5283\u7684\u65b9\u6cd5\u7a31\u70ba\u53d7\u6fc0\u653e\u5c04\u6d88\u53bb\u6cd5(stimulated emission depletion\uff0c\u7c21\u7a31STED)\uff0c\u5229\u7528\u4e00\u9053\u8108\u885d\u5149\u5c07\u6240\u6709\u7684\u87a2\u5149\u5206\u5b50\u6fc0\u767c(\u958b\u59cb\u767c\u5149)\uff0c\u540c\u6642\u5229\u7528\u53e6\u4e00\u9053\u8108\u885d\u5149\u5c07\u6240\u6709\u7684\u87a2\u5149\u5206\u5b50\u6dec\u6ec5\uff0c\u4f46\u662f\u53ea\u6709\u5728\u4e2d\u9593\u7684\u4e00\u500b\u5948\u7c73\u5c3a\u5ea6\u5927\u5c0f\u7684\u9ad4\u7a4d\u4e4b\u5167\u9664\u5916(\u5716\u4e8c)\uff0c\u56e0\u6b64\u53ea\u6703\u53d6\u5f97\u5728\u9019\u500b\u9ad4\u7a4d\u4e4b\u5167\u7684\u87a2\u5149\u3002\u900f\u904e\u5c0d\u6a23\u54c1\u7684\u6383\u63cf\u4ee5\u53ca\u540c\u6642\u5c0d\u5149\u7dda\u5f37\u5ea6\u7684\u6e2c\u91cf\uff0c\u5c31\u53ef\u4ee5\u53d6\u5f97\u4e00\u5f35\u6e05\u695a\u7684\u5716\u50cf\u3002\u6bcf\u4e00\u6b21\u88ab\u5bb9\u8a31\u653e\u51fa\u87a2\u5149\u7684\u9ad4\u7a4d\u6108\u5c0f\uff0c\u6700\u5f8c\u5f97\u5230\u7684\u5f71\u50cf\u89e3\u6790\u5ea6\u5c31\u6108\u9ad8\uff0c\u56e0\u6b64\u5728\u7406\u8ad6\u4e0a\uff0c\u5149\u5b78\u986f\u5fae\u93e1\u5728\u89e3\u6790\u5ea6\u65b9\u9762\u5c31\u4e0d\u518d\u6709\u9650\u5236\u4e86\u3002<\/p>\n<p><strong><img decoding=\"async\" class=\"aligncenter size-full wp-image-19423\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image004.jpg\" alt=\"image004\" width=\"666\" height=\"413\" srcset=\"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image004.jpg 666w, https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image004-300x186.jpg 300w\" sizes=\"(max-width: 666px) 100vw, 666px\" \/><\/strong><\/p>\n<p><strong>\u5728\u5fb7\u570b\u767c\u5c55\u982d\u4e00\u500b\u5948\u7c73\u624b\u96fb\u7b52<br \/>\n<\/strong>\u6d77\u723e\u7684\u7406\u8ad6\u6587\u7ae0\u4e26\u672a\u7acb\u523b\u7684\u6fc0\u8d77\u4e00\u5834\u9a37\u52d5\uff0c\u4f46\u662f\u7684\u78ba\u6709\u8da3\u5230\u8b93\u6d77\u723e\u5728\u4f4d\u65bc\u54e5\u5ef7\u6839\u7684\u99ac\u514b\u65af\uff65\u535c\u862d\u514b\u751f\u7269\u7269\u7406\u5316\u5b78\u7814\u7a76\u6240\uff0c\u5f97\u5230\u4e00\u500b\u8077\u4f4d\u3002\u5728\u63a5\u4e0b\u4f86\u7684\u6578\u5e74\u88e1\uff0c\u4ed6\u8b93\u81ea\u5df1\u7684\u60f3\u6cd5\u958b\u82b1\u7d50\u679c\uff1b\u4ed6\u8a2d\u8a08\u4e86\u4e00\u500bSTED\u986f\u5fae\u93e1\uff0c\u65bc2000\u5e74\uff0c\u5df2\u7d93\u80fd\u5920\u5c55\u793a\u771f\u7684\u53ef\u4ee5\u5be6\u969b\u7684\u904b\u7528\u4ed6\u7684\u60f3\u6cd5\uff0c\u5176\u4e2d\u4e4b\u4e00\u662f\u7528\u4f86\u53d6\u5f97\u4e00\u5f35\u5927\u8178\u687f\u83cc\u7684\u5716\u50cf\uff0c\u4e26\u5177\u6709\u7528\u5149\u5b78\u986f\u5fae\u93e1\u5f9e\u4f86\u7121\u6cd5\u9054\u5230\u7684\u89e3\u6790\u5ea6(\u5716\u4e09)\u3002<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<figure id=\"attachment_19424\" aria-describedby=\"caption-attachment-19424\" style=\"width: 587px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-19424 size-full\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image006.gif\" alt=\"image006\" width=\"587\" height=\"188\" \/><figcaption id=\"caption-attachment-19424\" class=\"wp-caption-text\">\u5716\u4e09 \u7531\u53f2\u8482\u82ac\uff65\u6d77\u723e\u900f\u904eSTED\u986f\u5fae\u93e1\uff0c\u6700\u5148\u6240\u53d6\u5f97\u7684\u5e7e\u500b\u5716\u50cf\u4e4b\u4e00\u3002\u5728\u5de6\u908a\u662f\u4e00\u500b\u666e\u901a\u7684\u5149\u5b78\u986f\u5fae\u93e1\u53d6\u5f97\u7684\u5927\u8178\u687f\u83cc\u5716\u50cf\uff0c\u5728\u53f3\u908a\u5247\u662f\u540c\u4e00\u500b\u5927\u8178\u687f\u83cc\uff0c\u7528STED\u986f\u5fae\u93e1\u7167\u51fa\u7684\u7d50\u679c\uff0c\u5176\u89e3\u6790\u5ea6\u9ad8\u4e86\u4e09\u500d\u3002\u6b64\u5716\u53d6\u81ea\u65bc Proc. Natl. Acad. Sci. USA 97:8206\uf02d8210\u3002<\/figcaption><\/figure>\n<p>STED\u986f\u5fae\u93e1\u5f9e\u6536\u96c6\u4e00\u5927\u5806\u5f88\u5c0f\u7684\u9ad4\u7a4d\u6240\u653e\u51fa\u7684\u5149\uff0c\u7136\u5f8c\u96c6\u5408\u6210\u4e00\u5f35\u6574\u9ad4\u7684\u5716\u50cf\uff0c\u76f8\u5c0d\u7684\u6bd4\u8f03\uff0c\u53e6\u4e00\u7a2e\u539f\u7406\u4e5f\u5f97\u5230\u4e86\u6210\u529f\uff0c\u90a3\u88ab\u7a31\u70ba\u55ae\u5206\u5b50\u986f\u5fae\u93e1\u8853\uff0c\u9700\u8981\u5c07\u8a31\u591a\u5f35\u5716\u50cf\u91cd\u758a\u5728\u4e00\u8d77\u3002\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c\u8207\u5a01\u5ec9\uff65\u83ab\u7d0d(\u5927\u5bb6\u90fd\u7528W. E.\u7a31\u547c\u4ed6)\u5404\u81ea\u7368\u7acb\u7684\uff0c\u4ee5\u4e0d\u540c\u7684\u57fa\u790e\u89c0\u5ff5\u5207\u5165\uff0c\u4fc3\u6210\u9019\u9805\u6280\u8853\u7684\u767c\u5c55\u3002\u9019\u9805\u6280\u8853\u7684\u57fa\u790e\uff0c\u662f\u5728\u83ab\u7d0d\u6210\u529f\u7684\u89c0\u6e2c\u5230\u4e00\u500b\u5c0f\u7684\u87a2\u5149\u5206\u5b50\u6642\u6240\u5960\u5b9a\u3002<\/p>\n<p><strong>W. E.<\/strong><strong>\u83ab\u7d0d\uff0d<\/strong><strong>\u9996\u5148\u89c0\u6e2c\u5230\u55ae\u4e00\u7684\u87a2\u5149\u5206\u5b50<br \/>\n<\/strong>\u5728\u5927\u90e8\u5206\u7684\u5316\u5b78\u65b9\u6cd5\u4e2d\uff0c\u4f8b\u5982\u91cf\u6e2c\u5438\u6536\u548c\u87a2\u5149\uff0c\u79d1\u5b78\u5bb6\u5011\u662f\u540c\u6642\u89c0\u5bdf\u4e0a\u767e\u842c\u7684\u5206\u5b50\uff0c\u5728\u9019\u4e9b\u5be6\u9a57\u4e2d\u6240\u5f97\u5230\u7684\u7d50\u679c\uff0c\u53cd\u6620\u7684\u53ea\u662f\u4e00\u7a2e\u5178\u578b\u5e73\u5747\u5316\u7684\u5206\u5b50\u8868\u73fe\uff0c\u4f46\u79d1\u5b78\u5bb6\u5011\u4e0d\u5f97\u4e0d\u63a5\u53d7\u9019\u7a2e\u56f0\u5883\uff0c\u56e0\u70ba\u6c92\u6709\u5225\u7684\u53ef\u80fd\u6027\u3002\u4e0d\u904e\u6709\u5f88\u9577\u7684\u4e00\u6bb5\u6642\u9593\uff0c\u4ed6\u5011\u5922\u60f3\u8457\u80fd\u5920\u91cf\u6e2c\u6bcf\u4e00\u500b\u55ae\u4e00\u7684\u5206\u5b50\uff0c\u56e0\u70ba\u6709\u6108\u8c50\u5bcc\u6108\u8a73\u76e1\u7684\u8cc7\u8a0a\uff0c\u5c31\u6108\u53ef\u80fd\u53bb\u77ad\u89e3\u8b6c\u5982\u75be\u75c5\u662f\u5982\u4f55\u7684\u767c\u5c55\u3002<\/p>\n<p>\u57281989\u5e74\uff0c\u83ab\u7d0d\u6210\u70ba\u5168\u7403\u7b2c\u4e00\u4f4d\u79d1\u5b78\u5bb6\u80fd\u5920\u91cf\u6e2c\u55ae\u4e00\u5206\u5b50\u5c0d\u5149\u7684\u5438\u6536\uff0c\u90a3\u662f\u4e00\u9805\u5177\u6709\u95dc\u9375\u6027\u7684\u6210\u5c31\u3002\u7576\u6642\u4ed6\u6b63\u5728\u4f4d\u65bc\u7f8e\u570b\u52a0\u5dde\u8056\u8377\u897f\u7684IBM\u7814\u7a76\u4e2d\u5fc3\u5de5\u4f5c\uff0c\u90a3\u500b\u5be6\u9a57\u6253\u958b\u4e86\u4e00\u6247\u901a\u5f80\u65b0\u672a\u4f86\u7684\u5927\u9580\uff0c\u4e26\u4e14\u555f\u767c\u4e86\u8a31\u591a\u5316\u5b78\u5bb6\u5c07\u6ce8\u610f\u529b\u8f49\u79fb\u5230\u55ae\u5206\u5b50\u7684\u8eab\u4e0a\uff0c\u5176\u4e2d\u4e4b\u4e00\u5c31\u662f\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c\uff0c\u63a5\u8457\u6703\u5728\u7a0d\u5f8c\u8aaa\u660e\u4ed6\u7684\u6210\u5c31\u3002<\/p>\n<p>\u516b\u5e74\u4e4b\u5f8c\uff0c\u83ab\u7d0d\u671d\u55ae\u5206\u5b50\u986f\u5fae\u93e1\u9081\u51fa\u4e86\u7b2c\u4e8c\u6b65\uff0c\u90a3\u662f\u904b\u7528\u4e4b\u524d\u8afe\u8c9d\u723e\u734e\u57282008\u5e74\u6240\u8868\u5f70\u904e\u7684\u7da0\u8272\u87a2\u5149\u86cb\u767d\u8cea(GFP)\u3002<\/p>\n<p><strong>\u5206\u5b50\u5927\u5c0f\u7684\u71c8\u4e00\u958b\u4e00\u95dc<br \/>\n<\/strong>\u57281997\u5e74\uff0c\u83ab\u7d0d\u9032\u5165\u4e86\u5728\u52a0\u5dde\u5927\u5b78\u7684\u8056\u5730\u7259\u54e5\u5206\u6821\uff0c\u90a3\u6b63\u662f\u5f8c\u4f86\u7372\u5f97\u8afe\u8c9d\u723e\u6842\u51a0\u7684\u9322\u6c38\u5065\u6240\u5728\u7684\u5b78\u6821\uff0c\u7576\u6642\u9322\u6c38\u5065\u6b63\u5617\u8a66\u8981\u8b93GFP\u653e\u51fa\u50cf\u5f69\u8679\u822c\u7684\u5404\u7a2e\u87a2\u5149\u3002\u9019\u500b\u7da0\u8272\u87a2\u5149\u86cb\u767d\u8cea\u662f\u5f9e\u4e00\u7a2e\u87a2\u5149\u6c34\u6bcd\u8eab\u4e0a\u5206\u96e2\u51fa\u4f86\u7684\uff0c\u5b83\u7684\u597d\u8655\u5728\u65bc\u80fd\u8b93\u7d30\u80de\u88e1\u9762\u7684\u5176\u5b83\u86cb\u767d\u8cea\u986f\u50cf\u3002\u79d1\u5b78\u5bb6\u5011\u5148\u5229\u7528\u57fa\u56e0\u79d1\u6280\uff0c\u5c07\u7da0\u8272\u87a2\u5149\u86cb\u767d\u8cea\u5076\u5408\u5230\u5176\u5b83\u7684\u86cb\u767d\u8cea\u4e0a\uff0c\u90a3\u7da0\u8272\u7684\u87a2\u5149\u5c31\u6703\u66b4\u9732\u51fa\u9019\u500b\u88ab\u6a19\u8a18\u7684\u86cb\u767d\u8cea\u4f4d\u5728\u4f55\u8655\u3002<\/p>\n<p>\u83ab\u7d0d\u767c\u73fe\u6709\u4e00\u7a2eGFP\u53ef\u96a8\u610f\u9ede\u4eae\u6216\u95dc\u6389\uff0c\u7576\u4ed6\u7528488\u5948\u7c73\u6ce2\u9577\u7684\u5149\u53bb\u6fc0\u767c\u86cb\u767d\u8cea\u7684\u6642\u5019\uff0c\u86cb\u767d\u8cea\u5c31\u958b\u59cb\u767c\u51fa\u87a2\u5149\uff0c\u4f46\u4e00\u500b\u77ed\u66ab\u7684\u6642\u9593\u4e4b\u5f8c\u5c31\u6703\u7184\u6ec5\uff0c\u5728\u9019\u4e4b\u5f8c\u7121\u8ad6\u4ed6\u518d\u7528\u591a\u5f37\u7684\u5149\u53bb\u7167\u5c04\u9019\u500b\u86cb\u767d\u8cea\uff0c\u5b83\u4e5f\u4e0d\u6703\u767c\u5149\uff0c\u4e0d\u904e\u4ed6\u5f8c\u4f86\u767c\u73fe\u7576\u5149\u7684\u6ce2\u9577\u6539\u70ba405\u5948\u7c73\u6642\uff0c\u9019\u500b\u86cb\u767d\u8cea\u5c31\u6703\u6062\u5fa9\u751f\u6a5f\uff0c\u7576\u86cb\u767d\u8cea\u91cd\u65b0\u6d3b\u5316\u5f8c\uff0c\u5b83\u53c8\u6703\u653e\u51fa488\u5948\u7c73\u6ce2\u9577\u7684\u87a2\u5149\u3002<\/p>\n<p>\u83ab\u7d0d\u5c07\u9019\u4e9b\u53ef\u88ab\u6fc0\u767c\u7684\u86cb\u767d\u8cea\u5747\u52fb\u7684\u6563\u4f48\u5728\u4e00\u500b\u81a0\u8cea\u5167\uff0c\u8b93\u6bcf\u500b\u86cb\u767d\u8cea\u4e4b\u9593\u7684\u8ddd\u96e2\u5927\u65bc0.2\u5fae\u7c73\u7684\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\uff0c\u56e0\u70ba\u5b83\u5011\u7a00\u758f\u7684\u6563\u958b\u4f86\uff0c\u4e00\u500b\u666e\u901a\u7684\u5149\u5b78\u986f\u5fae\u93e1\u5c31\u53ef\u4ee5\u5340\u8fa8\u6bcf\u4e00\u500b\u767c\u4eae\u7684\u5206\u5b50 \u2015 \u5b83\u5011\u5c31\u597d\u50cf\u4e00\u5806\u5177\u6709\u958b\u95dc\u7684\u5c0f\u71c8\u6ce1\uff0c\u9019\u9805\u7d50\u679c\u767c\u8868\u57281997\u5e74\u7684\u201c\u81ea\u7136\u201d\u671f\u520a\u4e0a\u3002<\/p>\n<p>\u900f\u904e\u9019\u500b\u767c\u73fe\uff0c\u83ab\u7d0d\u5c55\u793a\u4e86\u53ef\u4ee5\u900f\u904e\u5149\u5b78\u7684\u65b9\u5f0f\uff0c\u63a7\u5236\u55ae\u4e00\u5206\u5b50\u5011\u7684\u87a2\u5149\uff0c\u9019\u89e3\u6c7a\u4e86\u4e00\u500b\u8c9d\u9f4a\u683c\u5728\u5169\u5e74\u4e4b\u524d\u6240\u60f3\u5230\u7684\u554f\u984c\u3002<\/p>\n<p><strong>\u5c0d\u5b78\u8853\u611f\u5230\u75b2\u4e4f\uff0d<\/strong><strong>\u4f46\u4ecd\u7232\u963f\u8c9d\u7684\u7e5e\u5c04\u6975\u9650\u800c\u8457\u8ff7<br \/>\n<\/strong>\u8207\u6d77\u723e\u4e00\u6a23\uff0c\u8c9d\u9f4a\u683c\u4e5f\u7232\u4e86\u8d8a\u904e\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u7684\u60f3\u6cd5\u800c\u8457\u8ff7\u3002\u57281990\u5e74\u4ee3\u521d\u671f\uff0c\u4ed6\u6b63\u5728\u7f8e\u570b\u7d10\u6fa4\u897f\u5dde\u7684\u8c9d\u723e\u5be6\u9a57\u5ba4\uff0c\u7814\u7a76\u4e00\u7a2e\u65b0\u7684\u5149\u5b78\u986f\u5fae\u93e1\u8853\uff0c\u7a31\u70ba\u8fd1\u5834\u986f\u5fae\u93e1\u8853\u3002\u5728\u6b64\u6cd5\u4e2d\uff0c\u5149\u7dda\u662f\u5f9e\u4e00\u500b\u975e\u5e38\u8584\u7684\u5c16\u7aef\u6240\u91cb\u51fa\uff0c\u9019\u500b\u5c16\u7aef\u8207\u6a23\u54c1\u4e4b\u9593\u7684\u8ddd\u96e2\u53ea\u6709\u5e7e\u500b\u5948\u7c73\uff0c\u96d6\u7136\u9019\u7a2e\u986f\u5fae\u93e1\u8853\u4e5f\u53ef\u4ee5\u514b\u670d\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\uff0c\u4f46\u662f\u6b64\u6cd5\u5177\u6709\u4e00\u4e9b\u4e3b\u8981\u7684\u5f31\u9ede\uff0c\u8209\u4f8b\u4f86\u8aaa\uff0c\u56e0\u70ba\u653e\u51fa\u7684\u5149\u7bc4\u570d\u592a\u77ed(\u53ea\u80fd\u6df1\u5165\u7d04\u4e00\u767e\u5948\u7c73)\uff0c\u4ee5\u81f3\u65bc\u7121\u6cd5\u770b\u5230\u7d30\u80de\u8868\u9762\u4e4b\u4e0b\u7684\u7d50\u69cb\u3002<\/p>\n<p>\u8c9d\u9f4a\u683c\u57281995\u5e74\u5f97\u5230\u4e00\u500b\u7d50\u8ad6\uff0c\u90a3\u5c31\u662f\u8fd1\u5834\u986f\u5fae\u93e1\u8853\u7121\u6cd5\u66f4\u9032\u4e00\u6b65\u7684\u6539\u5584\uff0c\u6b64\u5916\u4ed6\u5728\u5b78\u8853\u754c\u611f\u89ba\u4e0d\u592a\u81ea\u5728\uff0c\u56e0\u6b64\u6c7a\u5b9a\u7d50\u675f\u4ed6\u7684\u7814\u7a76\u751f\u6daf\uff1b\u5373\u4fbf\u4e0d\u77e5\u4e0b\u4e00\u6b65\u8981\u4f55\u53bb\u4f55\u5f9e\uff0c\u4ed6\u6bc5\u7136\u8fad\u8077\uff0c\u4f46\u662f\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u4ecd\u5728\u4ed6\u7684\u5fc3\u4e2d\u3002\u6b65\u884c\u5728\u4e00\u500b\u5bd2\u51b7\u7684\u51ac\u5929\u88e1\uff0c\u4ed6\u60f3\u5230\u4e86\u4e00\u500b\u65b0\u7684\u9ede\u5b50\uff1b\u662f\u5426\u53ef\u80fd\u7528\u5177\u6709\u4e0d\u540c\u6027\u8cea\u7684\u5206\u5b50\uff0c\u90a3\u4e9b\u767c\u51fa\u4e0d\u540c\u984f\u8272\u4e4b\u87a2\u5149\u7684\u5206\u5b50\uff0c\u4f86\u514b\u670d\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\uff1f<\/p>\n<p>\u8c9d\u9f4a\u683c\u5df2\u7d93\u80fd\u7528\u8fd1\u5834\u986f\u5fae\u93e1\u8853\u89c0\u6e2c\u5230\u55ae\u5206\u5b50\u7684\u87a2\u5149\uff0c\u8207\u8a31\u591a\u4eba\u4e00\u6a23\uff0c\u8c9d\u9f4a\u683c\u53d7\u5230\u83ab\u7d0d\u7684\u555f\u767c\uff0c\u4ed6\u958b\u59cb\u4ed4\u7d30\u8003\u616e\uff0c\u5982\u679c\u4f7f\u7528\u5e7e\u7a2e\u6703\u653e\u51fa\u4e0d\u540c\u87a2\u5149\u7684\u5206\u5b50\uff0c\u4f8b\u5982\u7d05\u8272\u3001\u9ec3\u8272\u548c\u7da0\u8272\uff0c\u662f\u5426\u53ef\u4ee5\u5229\u7528\u666e\u901a\u7684\u5149\u5b78\u986f\u5fae\u93e1\u5f97\u5230\u76f8\u540c\u7684\u89e3\u6790\u5ea6\u3002\u4ed6\u7684\u9ede\u5b50\u662f\u8b93\u986f\u5fae\u93e1\u6bcf\u4e00\u6b21\u7528\u4e0d\u540c\u984f\u8272\u7684\u5149\u4f86\u8a18\u9304\u5f71\u50cf\uff0c\u5982\u679c\u540c\u4e00\u7a2e\u984f\u8272\u7684\u5206\u5b50\u90fd\u662f\u5747\u52fb\u7684\u6563\u4f48\uff0c\u800c\u4e14\u76f8\u4e92\u4e4b\u9593\u7684\u8ddd\u96e2\u5927\u65bc\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u7684\u898f\u7bc4\uff0c\u5b83\u5011\u7684\u4f4d\u7f6e\u5c07\u53ef\u7cbe\u78ba\u7684\u6c7a\u5b9a\u3002\u63a5\u8457\u7576\u9019\u4e9b\u5f71\u50cf\u91cd\u758a\u8d77\u4f86\u6642\uff0c\u5b8c\u6574\u7684\u5716\u50cf\u5c07\u5177\u6709\u9060\u8d85\u904e\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u7684\u89e3\u6790\u5ea6\uff0c\u7d05\u8272\u3001\u9ec3\u8272\u548c\u7da0\u8272\u7684\u5206\u5b50\u96d6\u7136\u76f8\u4e92\u7684\u8ddd\u96e2\u53ea\u4e0d\u904e\u5e7e\u500b\u5948\u7c73\uff0c\u4f46\u4ecd\u80fd\u5340\u5225\uff0c\u5982\u6b64\u5c31\u80fd\u514b\u670d\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u3002\u4e0d\u904e\uff0c\u4ecd\u6709\u4e00\u4e9b\u5be6\u969b\u7684\u56f0\u96e3\uff0c\u4f8b\u5982\u7f3a\u4e4f\u90a3\u4e9b\u5177\u6709\u4e0d\u540c\u5149\u5b78\u6027\u8cea\u4e4b\u5206\u5b50\uff0c\u5176\u5dee\u7570\u8981\u5927\u5230\u8db3\u4ee5\u76f8\u4e92\u5340\u5225\u3002<\/p>\n<p>\u57281995\u5e74\uff0c\u8c9d\u9f4a\u683c\u5728 <em>Optical Letters<\/em>\u9019\u4efd\u671f\u520a\u4e0a\u767c\u8868\u4e86\u4e0a\u8ff0\u60f3\u6cd5\u4e4b\u7406\u8ad6\uff0c\u96a8\u5373\u96e2\u958b\u4e86\u5b78\u8853\u754c\uff0c\u4e26\u9032\u5165\u4e86\u4ed6\u7236\u89aa\u958b\u7684\u516c\u53f8\u3002<\/p>\n<p><strong>\u88ab\u7da0\u8272\u87a2\u5149\u86cb\u767d\u8cea\u5f15\u8a98\u56de\u5230\u986f\u5fae\u93e1\u8853<br \/>\n<\/strong>\u8c9d\u9f4a\u683c\u5b8c\u5168\u7684\u812b\u96e2\u5b78\u8853\u754c\uff0c\u5df2\u7d93\u6709\u8a31\u591a\u5e74\u4e86\uff0c\u4f46\u662f\u6709\u4e00\u5929\uff0c\u4e00\u500b\u5c0d\u79d1\u5b78\u7684\u6e34\u671b\u7a81\u7136\u53c8\u5fa9\u7526\u4e86\u3002\u56de\u9867\u79d1\u5b78\u6587\u737b\u6642\uff0c\u4ed6\u7b2c\u4e00\u6b21\u770b\u5230\u7da0\u8272\u87a2\u5149\u86cb\u767d\u8cea\u7684\u8ad6\u6587\uff0c\u9ad4\u8a8d\u5230\u6709\u4e00\u500b\u86cb\u767d\u8cea\uff0c\u80fd\u8b93\u5176\u5b83\u7684\u86cb\u767d\u8cea\u5728\u7d30\u80de\u5167\u986f\u50cf\uff0c\u6d3b\u5316\u4e86\u8c9d\u9f4a\u683c\u5c0d\u5982\u4f55\u514b\u670d\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u7684\u60f3\u6cd5\u3002<\/p>\n<p>\u771f\u6b63\u7684\u7a81\u7834\u767c\u751f\u57282005\u5e74\uff0c\u7576\u6642\u4ed6\u5076\u7136\u767c\u73fe\u5230\u90a3\u7a2e\u53ef\u4ee5\u96a8\u610f\u6d3b\u5316\u7684\u87a2\u5149\u86cb\u767d\u8cea\uff0c\u5f88\u985e\u4f3c\u90a3\u4e9b\u83ab\u7d0d\u57281997\u5e74\uff0c\u65bc\u55ae\u5206\u5b50\u7684\u5c64\u6b21\u6240\u89c0\u5bdf\u5230\u7684\u87a2\u5149\u86cb\u767d\u8cea\u3002\u8c9d\u9f4a\u683c\u77e5\u9053\uff0c\u9019\u500b\u5206\u5b50\u6b63\u662f\u53ef\u4ee5\u5be6\u73fe\u4ed6\u5728\u5341\u5e74\u524d\u6240\u60f3\u5230\u7684\u90a3\u500b\u4e3b\u610f\uff0c\u6240\u9700\u8981\u7684\u5de5\u5177\u3002\u9019\u7a2e\u87a2\u5149\u5206\u5b50\u4e26\u4e0d\u9700\u8981\u5177\u6709\u4e0d\u540c\u7684\u984f\u8272\uff0c\u5b83\u5011\u9084\u662f\u53ef\u4ee5\u5728\u4e0d\u540c\u7684\u6642\u9593\u767c\u51fa\u87a2\u5149\u3002<\/p>\n<p><strong>\u85c9\u8457\u5f71\u50cf\u7684\u91cd\u758a\u8d85\u8d8a\u963f\u8c9d\u7e5e\u5c04\u6975\u9650<br \/>\n<\/strong>\u53ea\u4e0d\u904e\u4e00\u5e74\u4e4b\u5f8c\uff0c\u8207\u7814\u7a76\u53ef\u6fc0\u767c\u87a2\u5149\u86cb\u767d\u8cea\u7684\u79d1\u5b78\u5bb6\u5408\u4f5c\uff0c\u8c9d\u9f4a\u683c\u5c55\u793a\u4e86\u4ed6\u7684\u60f3\u6cd5\u7684\u78ba\u53ef\u4ee5\u4ed8\u8af8\u5be6\u73fe\u3002\u5728\u4e00\u4e9b\u4f8b\u5b50\u7576\u4e2d\uff0c\u4ed6\u5011\u5c07\u6703\u767c\u5149\u7684\u86cb\u767d\u8cea\u63a5\u5728\u6eb6\u9ad4(lysosome)\u7684\u819c\u4e0a\u9762\uff0c\u6eb6\u9ad4\u662f\u7d30\u80de\u88e1\u7684\u56de\u6536\u7ad9\uff0c\u73fe\u5728\u7528\u4e00\u9053\u8108\u885d\u5149\u4f86\u6fc0\u767c\u51fa\u86cb\u767d\u8cea\u7684\u87a2\u5149\uff0c\u56e0\u70ba\u4f7f\u7528\u7684\u8108\u885d\u5f88\u5f31\uff0c\u6240\u4ee5\u53ea\u80fd\u8b93\u90e8\u5206\u7684\u5206\u5b50\u958b\u59cb\u767c\u51fa\u87a2\u5149\uff0c\u7531\u65bc\u5b83\u5011\u7684\u6578\u76ee\u5f88\u5c11\uff0c\u5e7e\u4e4e\u6240\u6709\u767c\u5149\u5206\u5b50\u4e4b\u9593\u7684\u8ddd\u96e2\u5747\u5927\u65bc0.2\u5fae\u7c73\u7684\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\uff0c\u56e0\u6b64\u6bcf\u4e00\u500b\u767c\u5149\u7684\u86cb\u767d\u8cea\u4e4b\u4f4d\u7f6e\u90fd\u53ef\u4ee5\u5728\u986f\u5fae\u93e1\u4e0b\u8a18\u9304\u3002\u4e00\u6703\u5152\u4e4b\u5f8c\uff0c\u7576\u87a2\u5149\u6d88\u5931\u6642\uff0c\u4ed6\u5011\u91cd\u65b0\u6fc0\u767c\u53e6\u4e00\u7d44\u86cb\u767d\u8cea\uff0c\u540c\u6a23\u7684\uff0c\u4f7f\u7528\u7684\u8108\u885d\u5f31\u5230\u53ea\u80fd\u8b93\u90e8\u5206\u7684\u5206\u5b50\u767c\u51fa\u87a2\u5149\uff0c\u540c\u6642\u9019\u4e00\u7d44\u5716\u50cf\u88ab\u8a18\u9304\u4e0b\u4f86\uff0c\u9019\u500b\u6b65\u9a5f\u4e00\u76f4\u4e0d\u65b7\u7684\u91cd\u8907\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-19425\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image008.jpg\" alt=\"image008\" width=\"651\" height=\"465\" srcset=\"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image008.jpg 651w, https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image008-300x214.jpg 300w\" sizes=\"(max-width: 651px) 100vw, 651px\" \/><\/p>\n<p>\u7576\u8c9d\u9f4a\u683c\u5c07\u6240\u6709\u7684\u5f71\u50cf\u91cd\u758a\u8d77\u4f86\u6642\uff0c\u5f97\u5230\u4e86\u4e00\u5f35\u6eb6\u9ad4\u819c\u7684\u8d85\u9ad8\u89e3\u6790\u5716\u50cf\uff0c\u5b83\u7684\u89e3\u6790\u5ea6\u9060\u9060\u7684\u8d85\u904e\u4e86\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\u3002\u63a5\u8457\uff0c\u8c9d\u9f4a\u683c\u5c07\u9019\u4e00\u4efd\u958b\u5275\u6027\u7684\u5de5\u4f5c\uff0c\u65bc2006\u5e74\u767c\u8868\u5728\u201c\u79d1\u5b78\u201d\u671f\u520a\u4e0a\u3002<\/p>\n<figure id=\"attachment_19426\" aria-describedby=\"caption-attachment-19426\" style=\"width: 650px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-19426 size-full\" src=\"http:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image010.gif\" alt=\"image010\" width=\"650\" height=\"206\" \/><figcaption id=\"caption-attachment-19426\" class=\"wp-caption-text\">\u5716\u4e94 \u4e2d\u9593\u7684\u5716\u662f\u6eb6\u9ad4(lysosome)\u819c\u7684\u5716\u50cf\uff0c\u9019\u662f\u8c9d\u9f4a\u683c\u7528\u55ae\u5206\u5b50\u986f\u5fae\u93e1\uff0c\u6700\u521d\u6240\u53d6\u5f97\u7684\u5e7e\u500b\u5716\u50cf\u4e4b\u4e00\u3002\u5728\u5de6\u908a\u662f\u76f8\u540c\u7684\u5716\uff0c\u4f46\u662f\u7528\u50b3\u7d71\u7684\u986f\u5fae\u93e1\u6240\u53d6\u5f97\u7684\u3002\u5728\u53f3\u908a\u5247\u662f\u5c07\u819c\u7684\u5716\u50cf\u653e\u5927\uff0c\u8acb\u6ce8\u610f\u6b64\u5716\u7684\u5c3a\u5ea6\u662f0.2\u5fae\u7c73\uff0c\u7b49\u540c\u65bc\u963f\u8c9d\u7e5e\u5c04\u6975\u9650\uff0c\u5176\u89e3\u6790\u5ea6\u6539\u9032\u4e86\u8a31\u591a\u500d\u3002\u6b64\u5716\u53d6\u81ea\u65bc Science 313:1642-1645\u3002<\/figcaption><\/figure>\n<p><strong>\u9019\u5e7e\u4f4d\u5f97\u734e\u8005\u4ecd\u4f01\u5716\u5728\u63cf\u7e6a\u751f\u547d\u6700\u6df1\u5c64\u7684\u5967\u79d8<\/strong><\/p>\n<p>\u9019\u4e9b\u7531\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c\uff0c\u53f2\u8482\u82ac\uff65\u6d77\u723e\u4ee5\u53ca\u5a01\u5ec9\uff65\u83ab\u7d0d\u7b49\u4e09\u4eba\u6240\u958b\u767c\u7684\u65b9\u6cd5\uff0c\u767c\u5c55\u51fa\u4e86\u5e7e\u500b\u73fe\u5728\u7232\u5168\u4e16\u754c\u5404\u5730\u6240\u4f7f\u7528\u7684\u5948\u7c73\u986f\u5fae\u93e1\u6280\u8853\u3002\u9019\u4e09\u4f4d\u5f97\u734e\u8005\u4ecd\u7136\u6d3b\u8e8d\u5728\u9019\u500b\u4e0d\u50c5\u9f90\u5927\uff0c\u800c\u4e14\u4e00\u76f4\u5728\u589e\u9577\u7684\u79d1\u5b78\u793e\u7fa4\u4e2d\uff0c\u5c07\u5275\u65b0\u7684\u77db\u982d\u5c0d\u8457\u5948\u7c73\u986f\u5fae\u93e1\u8853\u7684\u9818\u57df\uff0c\u7576\u4ed6\u5011\u5c07\u529f\u80fd\u5f37\u5927\u7684\u5948\u7c73\u986f\u5fae\u93e1\u7784\u6e96\u5728\u751f\u547d\u4e2d\u6700\u5c0f\u7684\u96f6\u4ef6\u6642\uff0c\u4ed6\u5011\u4e5f\u540c\u6642\u53d6\u5f97\u4e86\u6700\u5c16\u7aef\u7684\u77e5\u8b58\u3002\u53f2\u8482\u82ac\uff65\u6d77\u723e\u7232\u4e86\u5c0d\u8166\u7a81\u89f8\u6709\u66f4\u597d\u7684\u77ad\u89e3\uff0c\u7aba\u63a2\u4e86\u6d3b\u7684\u795e\u7d93\u7d30\u80de\u5167\u90e8\uff1b\u5a01\u5ec9\uff65\u83ab\u7d0d\u7814\u7a76\u4e86\u8207\u676d\u4e01\u9813\u6c0f\u75c7(\u821e\u8e48\u75c7)\u6709\u95dc\u7684\u86cb\u767d\u8cea\uff1b\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c\u8ffd\u8e64\u4e86\u5728\u80da\u80ce\u4e2d\u7d30\u80de\u7684\u5206\u88c2\uff0c\u9019\u4e9b\u53ea\u662f\u773e\u591a\u4f8b\u5b50\u7576\u4e2d\u7684\u5e7e\u500b\u3002\u6709\u4e00\u4ef6\u4e8b\u60c5\u662f\u80af\u5b9a\u7684\uff0c2014\u5e74\u7684\u8afe\u8c9d\u723e\u5316\u5b78\u6842\u51a0\u5f97\u4e3b\u5011\uff0c\u5c0d\u767c\u5c55\u4eba\u985e\u6700\u91cd\u8981\u7684\u77e5\u8b58\uff0c\u5df2\u7d93\u5960\u5b9a\u4e86\u57fa\u77f3\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>--\u672c\u6587\u8b6f\u81ea\u8afe\u8c9d\u723e\u5316\u5b78\u734e\u59d4\u54e1\u6703\u516c\u4f48\u7d66\u5927\u773e\u7684\u65b0\u805e\u7a3f\uff0c<a href=\"http:\/\/www.ch.ntu.edu.tw\/nobel\/2014.html\" target=\"_blank\">\u51fa\u8655\u5728\u6b64<\/a><\/strong><br \/>\n\u539f\u6587\u53ef\u81ea\u4ee5\u4e0b\u5b98\u65b9\u7db2\u7ad9\u53d6\u5f97\uff1a<br \/>\n<a href=\"http:\/\/www.nobelprize.org\/nobel_prizes\/chemistry\/laureates\/2014\/popular-chemistryprize2014.pdf\" target=\"_blank\">http:\/\/www.nobelprize.org\/nobel_prizes\/chemistry\/laureates\/2014\/popular-chemistryprize2014.pdf<br \/>\n<\/a>\u82e5\u6709\u8208\u8da3\u95b1\u8b80\u9032\u968e\u7684\u8cc7\u6599\uff0c\u8acb\u7531\u4e0b\u5217\u7db2\u5740\u53d6\u5f97\uff1a<br \/>\n<a href=\"http:\/\/www.nobelprize.org\/nobel_prizes\/chemistry\/laureates\/2014\/advanced-chemistryprize2014.pdf\" target=\"_blank\">http:\/\/www.nobelprize.org\/nobel_prizes\/chemistry\/laureates\/2014\/advanced-chemistryprize2014.pdf<\/a><\/p>\n<p>*\u7279\u5225\u611f\u8b1d\u73fe\u65bc\u7f8e\u570b\u5fb7\u5dde\u8fb2\u5de5\u5927\u5b78\u653b\u8b80\u535a\u58eb\u7684\u66f9\u4e00\u5141(\u62112008\u5e74\u7684\u5c08\u984c\u751f)\u71b1\u8840\u76f8\u633a\uff0c\u5e6b\u6211\u5c07\u5716\u7247\u4e2d\u6587\u5316\uff1b\u53e6\u5916\u611f\u8b1d\u73fe\u65bc\u672c\u7cfb\u674e\u5f18\u6587\u6559\u6388\u5be6\u9a57\u5ba4\uff0c\u653b\u8b80\u78a9\u58eb\u5b78\u4f4d\u7684\u6797\u5b87\u8ed2\u5e6b\u6211\u6821\u7a3f\u3002\u591a\u5e74\u4f86\u5e6b\u6211\u5c07\u8b6f\u6587\u7f6e\u65bc\u53f0\u5927\u5316\u5b78\u7cfb\u7db2\u9801\u7684\u9ec3\u4fca\u8f1d\u5148\u751f\u696d\u5df2\u9000\u4f11\uff0c\u611f\u8b1d\u63a5\u66ff\u4ed6\u7684\u8521\u660e\u8ed2\u5e6b\u5fd9\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u25a0\u827e\u745e\u514b\uff65\u8c9d\u9f4a\u683c(Eric Betzig<\/p>\n","protected":false},"author":20,"featured_media":19422,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1027,32,3293,22],"tags":[1639,1469,1468,200,1104],"aioseo_notices":[],"jetpack_featured_media_url":"https:\/\/case.ntu.edu.tw\/blog\/wp-content\/uploads\/2014\/10\/image002.gif","_links":{"self":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/19420"}],"collection":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19420"}],"version-history":[{"count":10,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/19420\/revisions"}],"predecessor-version":[{"id":19436,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/posts\/19420\/revisions\/19436"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=\/wp\/v2\/media\/19422"}],"wp:attachment":[{"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19420"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=19420"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/case.ntu.edu.tw\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=19420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}